FastQCFastQC Report
Thu 26 May 2016
SRR1364230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918291
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23880.12448580533401866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.045.2996331
GTATCAA34800.038.3027271
GTACATG78800.037.3800541
TACATGG80700.035.536762
ATCAACG35800.034.0693443
CAACGCA35950.033.7608155
ACATGGG84550.033.1436463
TCAACGC37200.032.7854584
AACGCAG38250.032.0276376
CATGGGG41900.029.2498444
TATCAAC43600.028.7938842
ACGCAGA46150.026.2853437
CGCAGAG46150.026.1564948
AGTACAT55800.022.924962
GAGTACA58150.022.7176911
CATGGGA44600.022.6769374
GCAGAGT55200.021.2251249
ATGGGGA25600.021.1486725
AGAGTAC46600.019.3352510-11
CAGAGTA52800.019.20496710-11
Produced by FastQC (version 0.11.5)