FastQCFastQC Report
Thu 26 May 2016
SRR1364230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918291
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66160.3448903216456732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62440.3254980605132381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.19376622212166977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38900.071.003531
GTATCAA68650.046.82361
ATCAACG81850.038.3835953
TCAACGC83400.037.6702274
CAACGCA83500.037.619235
AACGCAG87200.036.0912256
TATCAAC89400.035.6744772
GTACATG74850.032.7655141
TGGTATC19350.031.9803162
GTGGTAT19850.031.487661
ACGCAGA100200.031.2297957
CGCAGAG101100.030.8929428
TACATGG78600.030.2051322
ACATGGG80050.028.9890273
GAGTACT76850.027.52061812-13
GCAGAGT115350.026.8702249
CAGAGTA111650.025.84247610-11
GTACTTT84050.025.1631114-15
GAGTACA57600.024.8027781
AGAGTAC104850.024.59641310-11
Produced by FastQC (version 0.11.5)