FastQCFastQC Report
Thu 26 May 2016
SRR1364229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036589
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60140.5801720836319891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.044.0093231
GTATCAA22800.040.1846051
GTACATG49600.035.984331
TCAACGC23600.035.540054
ATCAACG23900.035.3462453
CAACGCA24350.034.6930275
AACGCAG25450.033.412776
TACATGG52150.032.627632
ACATGGG52850.031.1808283
CGTTCGG400.00535566929.75853734-35
TGGACCG800.00536583329.7456075
TATCAAC29150.029.593972
ACGCAGA29900.028.238367
CGCAGAG30250.027.7150738
CATGGGG18550.027.578174
CATGGGA33950.025.0557374
GGACCGC1200.001292151624.7772456
CGGTATA1501.6875754E-423.7976362
TACCTGG5750.023.7976342
CCGGTCC1300.002053786322.8812373
Produced by FastQC (version 0.11.5)