FastQCFastQC Report
Thu 26 May 2016
SRR1364229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036589
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70650.6815623164050554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67820.6542612356488444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41660.4018950615914311No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16000.15435240003511516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.15232652478465428No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.14161832703221816No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.11518547852620468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.072.626081
GTATCAA54350.048.0874861
ATCAACG67700.038.0571063
TCAACGC68050.037.8613664
CAACGCA68950.037.3599555
TATCAAC70300.036.6495862
AACGCAG71500.036.5256
GTACATG48750.036.270111
TACATGG50800.034.436592
TGGTATC13600.033.689022
ACATGGG48700.033.6000753
ACGCAGA81000.032.0193337
CGCAGAG81650.031.7644338
GTGGTAT14500.031.6147541
GCAGAGT89100.028.9749589
CATGGGA31900.028.7254314
GAGTACT63350.028.64078912-13
CATGGGG16900.028.1669484
CAGAGTA88500.027.25576610-11
TAGACCG1150.001005614325.8657365
Produced by FastQC (version 0.11.5)