##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364229_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1036589 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35027383080469 33.0 31.0 34.0 30.0 34.0 2 32.314304898084 34.0 31.0 34.0 30.0 34.0 3 32.457996370789196 34.0 31.0 34.0 30.0 34.0 4 36.03814530156118 37.0 35.0 37.0 35.0 37.0 5 35.995673309286516 37.0 35.0 37.0 35.0 37.0 6 36.06288992069181 37.0 35.0 37.0 35.0 37.0 7 36.003798998445866 37.0 35.0 37.0 35.0 37.0 8 36.020719880299715 37.0 35.0 37.0 35.0 37.0 9 37.742513185071424 39.0 38.0 39.0 35.0 39.0 10-11 37.71391795591117 39.0 37.5 39.0 35.0 39.0 12-13 37.67617204118508 39.0 37.0 39.0 35.0 39.0 14-15 39.04513553587777 41.0 38.0 41.0 36.0 41.0 16-17 39.07801452649025 40.5 38.0 41.0 36.0 41.0 18-19 39.16113667036791 41.0 38.5 41.0 36.0 41.0 20-21 39.190787284063404 41.0 39.0 41.0 36.0 41.0 22-23 39.137039848966175 41.0 39.0 41.0 35.0 41.0 24-25 39.092462875836034 41.0 39.0 41.0 35.0 41.0 26-27 38.9885759929924 41.0 39.0 41.0 35.0 41.0 28-29 38.8918877202054 40.5 38.0 41.0 35.0 41.0 30-31 38.728064353374386 40.0 38.0 41.0 35.0 41.0 32-33 38.512979589789204 40.0 38.0 41.0 34.0 41.0 34-35 38.396376480938926 40.0 38.0 41.0 34.0 41.0 36-37 38.27448342592869 40.0 38.0 41.0 34.0 41.0 38-39 38.17372603799578 40.0 38.0 41.0 34.0 41.0 40-41 38.05372717634472 40.0 38.0 41.0 33.5 41.0 42-43 37.899963244834744 40.0 38.0 41.0 33.0 41.0 44-45 37.693521250949026 40.0 37.0 41.0 33.0 41.0 46-47 37.39877231959822 40.0 37.0 41.0 32.5 41.0 48-49 37.08915973447529 40.0 37.0 41.0 31.5 41.0 50-51 36.922356883972334 40.0 37.0 41.0 31.0 41.0 52-53 36.813821582131396 40.0 37.0 41.0 31.0 41.0 54-55 36.65701256717947 40.0 36.0 41.0 31.0 41.0 56-57 36.50913187386708 40.0 36.0 41.0 30.5 41.0 58-59 36.336329538515265 39.5 36.0 41.0 30.0 41.0 60-61 36.148485561780035 39.0 35.0 41.0 30.0 41.0 62-63 35.92645156373452 39.0 35.0 41.0 29.5 41.0 64-65 35.669969968811166 39.0 35.0 41.0 29.0 41.0 66-67 35.382994610207135 38.0 35.0 40.0 29.0 41.0 68-69 35.055751604541435 37.5 35.0 40.0 28.5 41.0 70-71 34.71706722722313 37.0 35.0 39.5 28.0 41.0 72-73 34.3692447054715 36.5 34.0 39.0 28.0 41.0 74-75 34.21675804007181 36.0 34.0 39.0 29.0 41.0 76-77 33.8973455246004 36.0 34.0 38.5 28.5 40.0 78-79 33.55362057671845 35.0 34.0 37.0 28.0 39.0 80-81 33.22167319931043 35.0 34.0 37.0 28.0 39.0 82-83 32.900043315142256 35.0 34.0 36.5 27.0 39.0 84-85 32.62967096891825 35.0 34.0 36.0 27.0 37.0 86-87 32.408298756787886 35.0 34.0 36.0 26.0 37.0 88-89 32.21320455841226 35.0 34.0 35.0 26.0 36.5 90-91 32.05551477007763 35.0 34.0 35.0 26.0 36.0 92-93 31.92317205758502 35.0 34.0 35.0 26.0 36.0 94-95 31.812937914641193 35.0 33.0 35.0 26.0 36.0 96-97 31.718064729608358 35.0 33.0 35.0 25.0 36.0 98-99 31.636537238963562 35.0 33.0 35.0 25.0 35.0 100-101 31.56729716406406 35.0 33.0 35.0 25.0 35.0 102-103 30.666248146565323 34.0 31.5 35.0 23.5 35.0 104-105 31.341863554407773 35.0 33.0 35.0 24.5 35.0 106-107 31.42869787350628 35.0 33.0 35.0 25.0 35.0 108-109 31.423521279890103 35.0 33.0 35.0 25.0 35.0 110-111 31.371016863964407 35.0 33.0 35.0 24.0 35.0 112-113 31.457425266909063 35.0 33.5 35.0 25.0 35.0 114-115 31.43388314944496 35.0 34.0 35.0 24.5 35.0 116-117 31.3799948677827 35.0 34.0 35.0 24.0 35.0 118-119 31.293068901946672 35.0 34.0 35.0 24.0 35.0 120-121 31.184101895736884 35.0 33.0 35.0 23.0 35.0 122-123 31.054802337281217 35.0 33.0 35.0 19.5 35.0 124-125 30.388996024460997 34.5 32.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14642470444118771 1101 2 0.28441691428349003 1101 3 0.1980451771333378 1101 4 0.12941659681035134 1101 5 0.040386553183452634 1101 6 0.06517025259899611 1101 7 0.058105997702199375 1101 8 0.091869700547349 1101 9 0.18068505154936076 1101 10-11 0.05093089372147119 1101 12-13 0.023094313816457657 1101 14-15 0.09751791860486492 1101 16-17 0.1589693718951395 1101 18-19 0.15908251833823073 1101 20-21 0.09218697857770763 1101 22-23 0.06715159404487281 1101 24-25 0.20218034679884767 1101 26-27 0.15401506663004483 1101 28-29 0.1907045333535322 1101 30-31 0.20064976931855938 1101 32-33 0.18862446832957147 1101 34-35 0.18814922725759686 1101 36-37 0.2042303226921831 1101 38-39 0.17624601456319766 1101 40-41 0.16978120121050466 1101 42-43 0.2637205835597669 1101 44-45 0.2828841091779424 1101 46-47 0.2191170352159233 1101 48-49 0.20503393127017233 1101 50-51 0.1694514331237258 1101 52-53 0.27356391216437714 1101 54-55 0.26359119586869184 1101 56-57 0.20939424521638017 1101 58-59 0.2780257410876885 1101 60-61 0.3285320462336472 1101 62-63 0.062001871456004665 1101 64-65 0.23422271034724673 1101 66-67 0.2841796769676961 1101 68-69 0.20770609181243316 1101 70-71 0.3042272777294812 1101 72-73 0.2315888075647763 1101 74-75 0.314742463646418 1101 76-77 0.34007422237597495 1101 78-79 0.2127151329317556 1101 80-81 0.29934577050111955 1101 82-83 0.3845397740557388 1101 84-85 0.3009923047887213 1101 86-87 0.175583112370731 1101 88-89 0.16031506539856366 1101 90-91 0.19429233428790837 1101 92-93 0.32993350497560314 1101 94-95 0.276077972680703 1101 96-97 0.36935746192107644 1101 98-99 0.29225733477074556 1101 100-101 0.3327764188477964 1101 102-103 0.307194164017762 1101 104-105 0.2648366122958876 1101 106-107 0.34081221027569697 1101 108-109 0.37955131133491804 1101 110-111 0.30143689791106 1101 112-113 0.4089874041163313 1101 114-115 0.4700589740560126 1101 116-117 0.32612196795979287 1101 118-119 0.4477490169259184 1101 120-121 0.46986955566339006 1101 122-123 0.40268596316597893 1101 124-125 0.3969386279644631 1102 1 0.20397604549226145 1102 2 0.24651689464410964 1102 3 0.1964355442835597 1102 4 0.10252259106715655 1102 5 0.12774065117864808 1102 6 0.1676347292095599 1102 7 0.14029812390378282 1102 8 0.15076925839593258 1102 9 0.24211915081846058 1102 10-11 0.15548537176517385 1102 12-13 0.24442546558638867 1102 14-15 0.27714193066450576 1102 16-17 0.2641355900408655 1102 18-19 0.24880528462431784 1102 20-21 0.33284964836544617 1102 22-23 0.27014713006720825 1102 24-25 0.281658788599934 1102 26-27 0.24826882332468614 1102 28-29 0.26417106944487756 1102 30-31 0.33055762347759554 1102 32-33 0.2902701951723614 1102 34-35 0.3052510123178678 1102 36-37 0.2834606182913717 1102 38-39 0.16411936180332987 1102 40-41 0.33302159251184804 1102 42-43 0.2470211403626834 1102 44-45 0.24009614015508873 1102 46-47 0.3317216679860806 1102 48-49 0.25724283056261754 1102 50-51 0.2741837271623808 1102 52-53 0.3174360279624011 1102 54-55 0.340052073695837 1102 56-57 0.3311159286826779 1102 58-59 0.3496922219764471 1102 60-61 0.23341110481737815 1102 62-63 0.20235442484061394 1102 64-65 0.16144753346014085 1102 66-67 0.17618233624571644 1102 68-69 0.23719700727286153 1102 70-71 0.3754976535847234 1102 72-73 0.2628280258113449 1102 74-75 0.2568719826939585 1102 76-77 0.32068431701654276 1102 78-79 0.15983809667321935 1102 80-81 0.16494607199211941 1102 82-83 0.2704927085504494 1102 84-85 0.1728858328173004 1102 86-87 0.11980462332897446 1102 88-89 0.02332243812742263 1102 90-91 0.09435642766518981 1102 92-93 0.012429776147644844 1102 94-95 -0.04394437374732263 1102 96-97 -0.03495961849952067 1102 98-99 -0.0750136408817319 1102 100-101 -0.06799628453919837 1102 102-103 0.023365334471645127 1102 104-105 0.012719407669486316 1102 106-107 -0.046682680831906964 1102 108-109 -0.009159098066508164 1102 110-111 0.019553428429063047 1102 112-113 -0.03469999536454793 1102 114-115 -0.07539291742845933 1102 116-117 -0.10957233715122427 1102 118-119 -0.09848048779018725 1102 120-121 -0.038259554672762874 1102 122-123 -0.0845650280918413 1102 124-125 -0.007290207951182737 1103 1 0.11141380850762772 1103 2 0.19414826114243766 1103 3 0.09437068219874334 1103 4 0.12487543270632528 1103 5 0.03699308216270225 1103 6 0.1279483491804072 1103 7 0.0809458945170789 1103 8 0.1156074616162357 1103 9 0.22003555022379118 1103 10-11 0.18309570745374515 1103 12-13 0.16267913316205806 1103 14-15 0.1792121692345745 1103 16-17 0.1903262418750984 1103 18-19 0.16572108261350849 1103 20-21 0.1282881382482728 1103 22-23 0.11822974622324978 1103 24-25 0.08288283507610572 1103 26-27 0.20487892019728093 1103 28-29 0.17203686737155266 1103 30-31 0.1724968758627341 1103 32-33 0.31685816509905607 1103 34-35 0.3078961180283244 1103 36-37 0.3540073985060701 1103 38-39 0.3330895814931907 1103 40-41 0.31211123269162044 1103 42-43 0.2913798527903211 1103 44-45 0.18233247460914015 1103 46-47 0.28786621958512626 1103 48-49 0.3595300457391417 1103 50-51 0.3634075419078755 1103 52-53 0.428166617287701 1103 54-55 0.42805096672336873 1103 56-57 0.43860124650172594 1103 58-59 0.48833468862452634 1103 60-61 0.4585082879142064 1103 62-63 0.43225355947655686 1103 64-65 0.41992664664849144 1103 66-67 0.4247191195320994 1103 68-69 0.4546245716375168 1103 70-71 0.3605614441235332 1103 72-73 0.38986520316493056 1103 74-75 0.3781750760068192 1103 76-77 0.27287965264468284 1103 78-79 0.3621089740030854 1103 80-81 0.4916317355733497 1103 82-83 0.45482904813091807 1103 84-85 0.41041353317785934 1103 86-87 0.41393858616954304 1103 88-89 0.40630573726380703 1103 90-91 0.4371592519454026 1103 92-93 0.29232376352831935 1103 94-95 0.39150112640543 1103 96-97 0.40120795612630644 1103 98-99 0.3338330530174538 1103 100-101 0.44387449476358753 1103 102-103 0.399984792263524 1103 104-105 0.421141192520885 1103 106-107 0.5012378488810931 1103 108-109 0.5961406159794969 1103 110-111 0.4388248771667733 1103 112-113 0.4747882507634422 1103 114-115 0.4120638258302023 1103 116-117 0.4753202811012365 1103 118-119 0.40894823106005873 1103 120-121 0.417751418358538 1103 122-123 0.3884043797070582 1103 124-125 0.4072553519263984 1104 1 0.11954227403617779 1104 2 0.22385945737570978 1104 3 0.1956654300192966 1104 4 0.09946479700623456 1104 5 0.12335856845785287 1104 6 0.14846782094679867 1104 7 0.1128181721032675 1104 8 0.20115465761102058 1104 9 0.20942542710005085 1104 10-11 0.20061744572008422 1104 12-13 0.22722296872287018 1104 14-15 0.2896029438971297 1104 16-17 0.3116051596235252 1104 18-19 0.3160156066355526 1104 20-21 0.2746934071045999 1104 22-23 0.26695314422773464 1104 24-25 0.31279411063255935 1104 26-27 0.28236872181157224 1104 28-29 0.22281644657851274 1104 30-31 0.21631666708205444 1104 32-33 0.32509723375162736 1104 34-35 0.4262848810965494 1104 36-37 0.41706133221850905 1104 38-39 0.39795636953525815 1104 40-41 0.3382122738299316 1104 42-43 0.3311224499417804 1104 44-45 0.3504382060111908 1104 46-47 0.3548171852454445 1104 48-49 0.31753131767506915 1104 50-51 0.339436907037296 1104 52-53 0.228927834647898 1104 54-55 0.31447559976725614 1104 56-57 0.3578060964487122 1104 58-59 0.2851383879533884 1104 60-61 0.3939517070715368 1104 62-63 0.4197562705492217 1104 64-65 0.3874752148132785 1104 66-67 0.3227467115551974 1104 68-69 0.2347866052142109 1104 70-71 0.2398901954920234 1104 72-73 0.19325562095958304 1104 74-75 0.22674730200701987 1104 76-77 0.24723290519740715 1104 78-79 0.253857246477331 1104 80-81 0.24322684000257766 1104 82-83 0.27816691215993217 1104 84-85 0.14911170848553468 1104 86-87 0.29764350567552356 1104 88-89 0.3318173415189207 1104 90-91 0.36897998674762533 1104 92-93 0.2715182789959165 1104 94-95 0.3156047010465528 1104 96-97 0.40064219522433575 1104 98-99 0.47728336067559596 1104 100-101 0.3903970202131042 1104 102-103 0.4092417552918377 1104 104-105 0.47523972953534255 1104 106-107 0.5007958684284652 1104 108-109 0.4047963473383369 1104 110-111 0.43877994464764214 1104 112-113 0.4386553766634975 1104 114-115 0.39212523648663833 1104 116-117 0.3711647575553023 1104 118-119 0.44502904392389553 1104 120-121 0.4815977197641459 1104 122-123 0.5339237434625907 1104 124-125 0.5873880929569602 1105 1 0.04497420688679199 1105 2 0.25092335156914913 1105 3 0.08128855712996597 1105 4 0.08695581084307236 1105 5 0.11345958404869094 1105 6 0.07832592809233319 1105 7 0.018765468101925364 1105 8 0.09229135681315626 1105 9 0.06470146803331289 1105 10-11 0.09940730908100193 1105 12-13 0.054964499907427466 1105 14-15 0.12781392130346347 1105 16-17 0.08380211490970169 1105 18-19 0.1757519960466638 1105 20-21 0.1458427384023011 1105 22-23 0.17004977811188837 1105 24-25 0.19959418836852905 1105 26-27 0.1078788435457696 1105 28-29 0.03236557793665895 1105 30-31 0.13738727423788077 1105 32-33 0.19223665700669557 1105 34-35 0.0931852085922813 1105 36-37 0.13562684824455573 1105 38-39 0.10347517068845491 1105 40-41 0.0789002207191345 1105 42-43 0.029511610714429537 1105 44-45 0.10894387192602295 1105 46-47 0.18974867630210923 1105 48-49 0.17748572190377132 1105 50-51 0.18444834097778084 1105 52-53 0.23773015717477364 1105 54-55 0.22609189862914292 1105 56-57 0.13878087590170907 1105 58-59 0.29917883042259774 1105 60-61 0.23292851556517746 1105 62-63 0.26951297507100236 1105 64-65 0.25618397419162164 1105 66-67 0.20394551718808884 1105 68-69 0.10735190054914057 1105 70-71 0.10775654067433038 1105 72-73 0.14169871929806277 1105 74-75 0.1577809186978527 1105 76-77 0.27438017616244537 1105 78-79 0.19640406304925762 1105 80-81 0.20292703771875154 1105 82-83 0.09641502002189384 1105 84-85 0.0786599834304269 1105 86-87 0.06794832291775776 1105 88-89 -0.031819639723160265 1105 90-91 0.012509290491877323 1105 92-93 -0.01721609866751095 1105 94-95 -0.0402063923049667 1105 96-97 0.07434565554563832 1105 98-99 0.06421157101069142 1105 100-101 0.01821381694753299 1105 102-103 0.15964258140124343 1105 104-105 0.09092448546168086 1105 106-107 0.12474851206914295 1105 108-109 0.09790600858572063 1105 110-111 0.08805071699241296 1105 112-113 0.019594870243281548 1105 114-115 0.029962274219052176 1105 116-117 0.013140577056322655 1105 118-119 0.08338178242881256 1105 120-121 0.13479337643136802 1105 122-123 0.14986789378946597 1105 124-125 0.13576473692043933 1106 1 0.011306773959255167 1106 2 0.06965337889337064 1106 3 0.015933403840833193 1106 4 -0.0071747787343028335 1106 5 -0.06025495790716917 1106 6 -0.08717902796470156 1106 7 -0.024316378907066394 1106 8 -0.021890136138964067 1106 9 0.10792375513721453 1106 10-11 0.13408862093932328 1106 12-13 0.13114832367234897 1106 14-15 0.1206471647058649 1106 16-17 0.11995188811126667 1106 18-19 0.08659286263596755 1106 20-21 4.8354438060727034E-4 1106 22-23 0.1648873726933644 1106 24-25 0.07264065432905653 1106 26-27 0.09965262658307239 1106 28-29 0.08829461380309311 1106 30-31 -0.02432087153400886 1106 32-33 0.05218400429041736 1106 34-35 0.012687163666228685 1106 36-37 0.05229536280720026 1106 38-39 0.03779293980986864 1106 40-41 0.04955686439797802 1106 42-43 0.025975969728584403 1106 44-45 0.06404184737117902 1106 46-47 0.005084734666056079 1106 48-49 -0.024282960940603004 1106 50-51 0.01531042141299821 1106 52-53 0.08397104396774324 1106 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-0.46681921410393556 2216 38-39 -0.43970085590729724 2216 40-41 -0.542256954676489 2216 42-43 -0.5948144367706405 2216 44-45 -0.6301387408416019 2216 46-47 -0.7125934675021526 2216 48-49 -0.7453418063132915 2216 50-51 -0.7136619849416661 2216 52-53 -0.6597371541894645 2216 54-55 -0.6460803718508075 2216 56-57 -0.567521854756869 2216 58-59 -0.47889732678910235 2216 60-61 -0.5049593144479729 2216 62-63 -0.5438948271689341 2216 64-65 -0.5465224290255293 2216 66-67 -0.5941609476739842 2216 68-69 -0.6286885620121225 2216 70-71 -0.5873060579558853 2216 72-73 -0.6732805662941246 2216 74-75 -0.6351103136658551 2216 76-77 -0.7262428730960622 2216 78-79 -0.527619105935031 2216 80-81 -0.499540540949468 2216 82-83 -0.4419789055118457 2216 84-85 -0.5094303705922343 2216 86-87 -0.614222139135304 2216 88-89 -0.5806060796896588 2216 90-91 -0.5959667864626219 2216 92-93 -0.4483283999963099 2216 94-95 -0.4735508468035583 2216 96-97 -0.4837840482617608 2216 98-99 -0.6342246677518126 2216 100-101 -0.6951587737691618 2216 102-103 -0.7814797586767313 2216 104-105 -0.7546003840378752 2216 106-107 -0.6705999368088058 2216 108-109 -0.6201881272931402 2216 110-111 -0.6558516173962765 2216 112-113 -0.5064675970449812 2216 114-115 -0.6553074288887366 2216 116-117 -0.5887542121002447 2216 118-119 -0.5834360264838274 2216 120-121 -0.6056285612386283 2216 122-123 -0.680320657812409 2216 124-125 -0.7314685151656839 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42.0 3 4.0 4 8.0 5 106.0 6 347.0 7 620.0 8 964.0 9 1550.0 10 2688.0 11 3880.0 12 4597.0 13 6649.0 14 10443.0 15 10225.0 16 4121.0 17 3037.0 18 2902.0 19 2681.0 20 2667.0 21 2648.0 22 2861.0 23 3114.0 24 3692.0 25 4705.0 26 5991.0 27 7728.0 28 10121.0 29 12815.0 30 16695.0 31 20738.0 32 26681.0 33 34775.0 34 47511.0 35 75007.0 36 134899.0 37 349011.0 38 218658.0 39 1408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.864436551750764 20.5081457746207 13.732482724008802 22.894934949619735 2 15.611397104223265 21.810651549765375 40.17446437391949 22.403486972091873 3 18.090153638964832 27.351999737600156 31.330733774139336 23.22711284929567 4 11.96297825860412 18.258835698654142 39.11231960971242 30.665866433029322 5 11.6361071932299 39.10924424875405 35.941452383754694 13.313196174261355 6 28.867756699184984 39.208948197085704 18.10709434428254 13.816200759446776 7 25.454375315940375 34.3691032502788 23.35035713321011 16.826164300570717 8 24.751661458881003 36.02276312019518 21.15968816956383 18.06588725135999 9 26.025165229420725 15.514442078779537 21.893633831730803 36.56675886006894 10-11 23.28584424492253 27.511964722758968 29.461097889327398 19.741093142991097 12-13 24.489021203196252 25.91750443039623 29.435099156946485 20.15837520946103 14-15 21.845688117469894 26.759255596962735 28.12035435452238 23.274701931044994 16-17 20.055489687812624 29.739800441640806 29.075988651239786 21.128721219306783 18-19 19.727265404208378 27.803184620898335 32.46415721768457 20.00539275720871 20-21 20.6975956719587 27.047798114778377 32.64389261317648 19.610713600086438 22-23 20.44315538752582 27.19578347831204 32.39007938536874 19.9709817487934 24-25 20.19218803209372 27.283233759956936 32.69555243206324 19.8290257758861 26-27 20.02002731084634 27.38942170175224 32.55986366765083 20.030687319750584 28-29 19.887071816382207 27.084772349284382 33.0027937811358 20.025362053197608 30-31 20.152876773911295 27.03647983067501 32.91399222351439 19.896651171899308 32-33 19.993604022423543 27.30928072746286 32.56372583540825 20.133389414705345 34-35 20.323725217998646 27.193419957186503 32.55007529503014 19.932779529784707 36-37 20.57503021930582 26.81564245810056 32.29061855759612 20.318708764997506 38-39 20.1621857843369 27.369381693226536 32.26100219083938 20.20743033159719 40-41 20.320455031094788 27.10357099273241 32.512660520544074 20.06331345562873 42-43 20.342984267989262 27.078218202902132 32.11454547734839 20.464252051760216 44-45 20.309688796620453 27.36301465672509 31.887758793504467 20.439537753149995 46-47 20.574258457305643 27.420269750113114 31.305078483371908 20.70039330920934 48-49 20.625482364744926 27.71161341359883 30.93699351701783 20.72591070463842 50-51 20.730897907365318 27.69943314026402 30.646891532605046 20.922777419765616 52-53 20.79700958599611 27.908070528850608 30.609679386249265 20.68524049890402 54-55 20.73261437271667 27.801616648449865 30.609817391463736 20.855951587369727 56-57 20.73261437271667 28.04636167275555 30.369992349909175 20.85103160461861 58-59 20.724266686732662 27.737380490353125 30.567554152865267 20.970798670048946 60-61 20.75089548509583 27.963734903611748 30.567804597579173 20.717565013713248 62-63 20.716386031731332 27.77436912610532 30.817458985237966 20.69178585692538 64-65 20.640147638070633 27.547610480142083 30.718249952488403 21.093991929298884 66-67 20.697985036218025 27.884575033319038 30.294061324397447 21.123378606065486 68-69 20.65793104246702 27.67953791622368 30.536399613322246 21.12613142798706 70-71 20.601468721764395 27.567839016594153 30.700607199660002 21.13008506198145 72-73 20.822186999862048 27.65064070716552 30.345392436153578 21.181779856818856 74-75 20.489509342661364 27.92003388035181 30.193644732869053 21.396812044117773 76-77 20.57285524168672 27.80539263992776 30.41734859699618 21.204403521389338 78-79 20.67365598010771 27.788670945149086 30.148396292625044 21.389276782118163 80-81 20.571394117717993 27.905844899731093 29.969298298503507 21.553462684047414 82-83 20.808992319406197 27.742026976379385 30.09651510481408 21.352465599400333 84-85 20.53883325149314 27.753616966677697 29.995861362393196 21.71168841943597 86-87 20.54932089767497 27.59073268190189 30.21510936349894 21.644837056924203 88-89 20.768453070599826 27.368851106851416 30.1736271559895 21.689068666559265 90-91 20.551266863211186 27.517615688574693 30.260058114407435 21.671059333806685 92-93 20.63886389893177 27.5832044977099 30.038035652696145 21.739895950662184 94-95 20.51023789780481 27.269319880580774 29.98939235410145 22.231049867512965 96-97 20.811571105807356 27.27097325633669 29.843369844353845 22.074085793502107 98-99 20.546145782630948 27.253983790352265 29.83897927748831 22.360891149528477 100-101 20.286093479459616 27.351592203660815 29.9695245942844 22.39278972259517 102-103 20.586171924078652 27.114938679877987 29.854393056722923 22.444496339320434 104-105 20.477089128226204 26.991682368957033 29.885583126622688 22.645645376194075 106-107 20.41775655974496 27.035524603476752 29.911684281075885 22.6350345557024 108-109 20.510426572984564 27.107642261359366 29.478548313398868 22.903382852257206 110-111 20.401479312066815 27.052593741631824 29.56259936263169 22.98332758366967 112-113 20.325919379767182 27.015312866010493 29.737128490305004 22.921639263917324 114-115 20.34849198880357 27.171888724512954 29.353388376493672 23.1262309101898 116-117 20.195488917647978 27.226105449600873 29.315909377033428 23.262496255717718 118-119 20.159391108791354 26.957608583444205 29.395652899594506 23.48734740816994 120-121 20.01415218567822 27.176585898557676 29.429262706820158 23.37999920894395 122-123 20.141213560669353 27.288088011993217 29.1704209780954 23.400277449242033 124-125 20.065223884990544 27.140973059678696 29.073030386821646 23.72077266850911 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 716.0 1 544.5 2 857.5 3 1189.5 4 640.0 5 383.0 6 922.5 7 2190.0 8 3614.0 9 4034.5 10 2843.0 11 1461.0 12 1145.5 13 1191.0 14 1251.5 15 1338.5 16 1493.0 17 1648.5 18 1804.0 19 1939.5 20 2114.0 21 2483.5 22 2989.5 23 3602.0 24 4374.5 25 5586.0 26 7593.0 27 9612.0 28 10936.0 29 12707.0 30 15735.0 31 18787.0 32 22106.0 33 26102.5 34 30253.0 35 33371.0 36 36560.5 37 39648.0 38 39980.5 39 40109.0 40 40098.0 41 39492.5 42 39060.0 43 39217.0 44 39993.5 45 40083.0 46 38344.0 47 35918.5 48 34738.0 49 33677.0 50 31707.0 51 28907.0 52 26430.5 53 24516.5 54 22588.5 55 20374.5 56 18177.0 57 16146.5 58 14310.5 59 12652.0 60 10696.5 61 8400.0 62 6344.5 63 4710.5 64 3442.5 65 2660.5 66 2089.0 67 1642.5 68 1248.5 69 847.0 70 601.0 71 481.0 72 386.0 73 332.0 74 281.0 75 208.0 76 135.5 77 68.5 78 33.5 79 25.0 80 14.0 81 4.0 82 2.0 83 1.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04524454726029314 2 0.004341161250987614 3 2.8941075006584096E-4 4 0.021705806254938072 5 0.0029905777506803563 6 0.004341161250987614 7 9.647025002194698E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0013023483752962841 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.341161250987614E-4 28-29 9.647025002194698E-5 30-31 2.4117562505486748E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.823512501097349E-5 42-43 0.0038588100008778796 44-45 0.0 46-47 0.0 48-49 0.0027976372506364625 50-51 9.647025002194698E-5 52-53 0.007428209251689918 54-55 0.0 56-57 0.0 58-59 9.164673752084963E-4 60-61 0.0 62-63 0.0010611727502414167 64-65 0.0 66-67 0.001881169875427966 68-69 0.0064635067514704474 70-71 0.010563492377403194 72-73 0.0 74-75 0.0 76-77 9.164673752084963E-4 78-79 6.270566251426554E-4 80-81 1.4470537503292048E-4 82-83 0.0014952888753401782 84-85 0.0015435240003511517 86-87 0.0 88-89 0.0 90-91 0.0012541132502853108 92-93 0.005932920376349739 94-95 0.006994093126591157 96-97 6.752917501536288E-4 98-99 0.0103705518773593 100-101 0.017316409878939485 102-103 0.029519896506715774 104-105 0.01119054900254585 106-107 0.035066935882977726 108-109 0.030725774631990114 110-111 0.02860342913150728 112-113 0.0072352687516460245 114-115 8.199971251865494E-4 116-117 8.199971251865494E-4 118-119 0.007910560501799653 120-121 0.0 122-123 2.8941075006584096E-4 124-125 0.044906901385216316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1036589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.5618307165365 #Duplication Level Percentage of deduplicated Percentage of total 1 86.35465883548946 47.11668276966239 2 7.982639328481104 8.710948314235052 3 1.9595734164399883 3.2075373907327123 4 0.8419978937924091 1.8376378617912679 5 0.46024392119889584 1.2555875458384558 6 0.316019974445988 1.0345577009259712 7 0.22468405515181936 0.8581421367328961 8 0.1854245882772255 0.8093683997012366 9 0.1461015343794211 0.7174410467612591 >10 1.2547208048914915 14.625778900740812 >50 0.1791117701548857 6.760385461649862 >100 0.09163115415602419 8.60900450274104 >500 9.578169423974649E-4 0.30154161866136653 >1k 0.0015963615706624415 1.743227601354301 >5k 6.385446282649765E-4 2.4121587484713705 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7065 0.6815623164050554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6782 0.6542612356488444 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4166 0.4018950615914311 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1600 0.15435240003511516 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1579 0.15232652478465428 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1468 0.14161832703221816 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1194 0.11518547852620468 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.647025002194698E-5 0.0 0.0 0.0 0.0 7 9.647025002194698E-5 0.0 0.0 0.0 0.0 8 9.647025002194698E-5 0.0 0.0 0.0 0.0 9 9.647025002194698E-5 0.0 0.0 0.0 0.0 10-11 9.647025002194698E-5 0.0 0.0 0.0 0.0 12-13 9.647025002194698E-5 0.0 0.0 4.823512501097349E-5 0.0 14-15 9.647025002194698E-5 0.0 0.0 9.647025002194698E-5 0.0 16-17 9.647025002194698E-5 0.0 0.0 9.647025002194698E-5 0.0 18-19 9.647025002194698E-5 0.0 0.0 9.647025002194698E-5 0.0 20-21 9.647025002194698E-5 0.0 0.0 1.4470537503292048E-4 0.0 22-23 9.647025002194698E-5 0.0 0.0 3.376458750768144E-4 0.0 24-25 9.647025002194698E-5 0.0 0.0 5.305863751207084E-4 0.0 26-27 9.647025002194698E-5 0.0 0.0 9.647025002194698E-4 0.0 28-29 9.647025002194698E-5 0.0 0.0 0.0030388128756913303 0.0 30-31 9.647025002194698E-5 0.0 0.0 0.011528194877622664 0.0 32-33 9.647025002194698E-5 0.0 0.0 0.02865166425651825 0.0 34-35 9.647025002194698E-5 0.0 0.0 0.05354098876218057 0.0 36-37 9.647025002194698E-5 0.0 0.0 0.09386555327135442 0.0 38-39 9.647025002194698E-5 0.0 0.0 0.15107241153436896 0.0 40-41 9.647025002194698E-5 0.0 0.0 0.21165572854815168 0.0 42-43 9.647025002194698E-5 0.0 0.0 0.27180492943683565 0.0 44-45 9.647025002194698E-5 0.0 0.0 0.34931877532947 0.0 46-47 1.4470537503292048E-4 0.0 0.0 0.4407725723502758 0.0 48-49 1.9294050004389397E-4 0.0 0.0 0.5414392782481774 0.0 50-51 1.9294050004389397E-4 0.0 0.0 0.6450000916467376 0.0 52-53 1.9294050004389397E-4 0.0 0.0 0.744316214044332 0.0 54-55 1.9294050004389397E-4 0.0 0.0 0.8615275678209975 0.0 56-57 1.9294050004389397E-4 0.0 0.0 1.006232942853918 0.0 58-59 1.9294050004389397E-4 0.0 0.0 1.1604406375140002 0.0 60-61 1.9294050004389397E-4 0.0 0.0 1.3271894646769355 0.0 62-63 1.9294050004389397E-4 0.0 0.0 1.4973147505906392 0.0 64-65 1.9294050004389397E-4 0.0 0.0 1.6787270557569105 0.0 66-67 1.9294050004389397E-4 0.0 0.0 1.8900451384299854 0.0 68-69 1.9294050004389397E-4 0.0 0.0 2.135417219360807 0.0 70-71 1.9294050004389397E-4 0.0 0.0 2.4114668397986088 0.0 72-73 1.9294050004389397E-4 0.0 0.0 2.6842847068606748 0.0 74-75 1.9294050004389397E-4 0.0 0.0 2.9635660806742115 0.0 76-77 1.9294050004389397E-4 0.0 0.0 3.277190863495561 0.0 78-79 1.9294050004389397E-4 0.0 0.0 3.6346613749518855 0.0 80-81 1.9294050004389397E-4 0.0 0.0 4.020735315539717 0.0 82-83 1.9294050004389397E-4 0.0 0.0 4.428370357007454 0.0 84-85 1.9294050004389397E-4 0.0 0.0 4.824621908972602 0.0 86-87 1.9294050004389397E-4 0.0 0.0 5.255506280695627 0.0 88-89 1.9294050004389397E-4 0.0 0.0 5.737037533680176 0.0 90-91 1.9294050004389397E-4 0.0 0.0 6.256819240798427 0.0 92-93 1.9294050004389397E-4 0.0 0.0 6.8038537935478764 0.0 94-95 1.9294050004389397E-4 0.0 0.0 7.3590400824241815 0.0 96-97 1.9294050004389397E-4 0.0 0.0 7.914612252300575 0.0 98-99 1.9294050004389397E-4 0.0 0.0 8.50843487631067 0.0 100-101 2.4117562505486745E-4 0.0 0.0 9.157438483333317 0.0 102-103 2.8941075006584096E-4 0.0 0.0 9.848503119365535 0.0 104-105 2.8941075006584096E-4 0.0 0.0 10.546368908024299 0.0 106-107 2.8941075006584096E-4 0.0 0.0 11.244717047933172 0.0 108-109 2.8941075006584096E-4 0.0 0.0 11.960526303096021 0.0 110-111 2.8941075006584096E-4 0.0 0.0 12.724667153519862 0.0 112-113 2.8941075006584096E-4 0.0 0.0 13.521607888951166 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3115 0.0 72.62608 1 GTATCAA 5435 0.0 48.087486 1 ATCAACG 6770 0.0 38.057106 3 TCAACGC 6805 0.0 37.861366 4 CAACGCA 6895 0.0 37.359955 5 TATCAAC 7030 0.0 36.649586 2 AACGCAG 7150 0.0 36.525 6 GTACATG 4875 0.0 36.27011 1 TACATGG 5080 0.0 34.43659 2 TGGTATC 1360 0.0 33.68902 2 ACATGGG 4870 0.0 33.600075 3 ACGCAGA 8100 0.0 32.019333 7 CGCAGAG 8165 0.0 31.764433 8 GTGGTAT 1450 0.0 31.614754 1 GCAGAGT 8910 0.0 28.974958 9 CATGGGA 3190 0.0 28.725431 4 GAGTACT 6335 0.0 28.640789 12-13 CATGGGG 1690 0.0 28.166948 4 CAGAGTA 8850 0.0 27.255766 10-11 TAGACCG 115 0.0010056143 25.865736 5 >>END_MODULE