FastQCFastQC Report
Thu 26 May 2016
SRR1364228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103295
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46330.22027342812111472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.054.2023161
GTATCAA38250.048.216951
CAACGCA44800.039.9664885
ATCAACG44800.039.9655383
TCAACGC44950.039.8321724
AACGCAG46950.038.0005536
GTACATG111750.035.616241
TATCAAC50900.035.5273932
TACATGG109050.034.638222
ACATGGG108950.032.867483
ACGCAGA61150.029.0789687
TACCGTA1454.084548E-628.7100737
CGCAGAG63200.027.8534458
CATGGGA65600.027.293544
CATGGGG46900.025.873464
GCAGAGT70400.024.1611889
ATGGGAG31500.023.9828455
GAGTACA84500.023.3045521
ATGGGAT25550.022.1177145
AGTACAT83500.021.8705352
Produced by FastQC (version 0.11.5)