FastQCFastQC Report
Thu 26 May 2016
SRR1364228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103295
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102240.4860944375372927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92370.4391680672468674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55190.2623978091518308No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24810.11795777577562824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.076.9402851
GTATCAA91100.054.5048981
ATCAACG119050.041.3926773
TCAACGC120750.040.9084974
CAACGCA121600.040.564925
TATCAAC121700.040.3945122
AACGCAG124600.040.0629166
TGGTATC21100.035.8223572
ACGCAGA147050.033.863217
CGCAGAG149350.033.3815468
GTACATG105750.033.273231
GTACCGT1451.09896064E-732.8262026
TACATGG104500.032.520162
ACATGGG103750.031.2630233
GCAGAGT162100.030.5724099
GTGGTAT26350.030.5028921
GAGTACT110850.027.53404612-13
CAGAGTA160800.026.89775710-11
AGAGTAC156500.026.05918310-11
AGTACTT114650.025.79114712-13
Produced by FastQC (version 0.11.5)