FastQCFastQC Report
Thu 26 May 2016
SRR1364227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504918
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.058.108161
GTATCAA38400.044.158451
GTACATG110850.036.1226461
ATCAACG45950.035.8605043
TCAACGC46900.034.752914
CAACGCA47700.034.1714175
TACATGG114800.034.1493952
AACGCAG48600.033.5258946
ACATGGG115300.032.1941833
TATCAAC54550.030.4262872
CATGGGG66300.026.6474934
ACGCAGA61350.026.4609537
CGCAGAG62450.025.709218
CATGGGA60550.024.167654
GAGTACA80300.022.5246581
ATGGGGA42050.020.9375825
AGTACAT82400.020.8646032
GTATAGA7300.020.3759251
GCAGAGT76950.020.0158739
ATGGGAG31450.019.482625
Produced by FastQC (version 0.11.5)