FastQCFastQC Report
Thu 26 May 2016
SRR1364227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504918
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60560.24176440107021466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57130.22807133806376098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.12902617969929553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40800.075.739241
GTATCAA74350.050.851821
ATCAACG95600.039.1536523
TCAACGC97500.038.3891264
CAACGCA98550.038.094035
AACGCAG102400.036.9500776
TATCAAC105200.035.8649672
GTACATG105950.034.898311
GTGGTAT21050.032.8110121
TACATGG110950.032.397132
TGGTATC20750.031.8347742
ACATGGG111300.031.3850273
ACGCAGA121150.031.0337687
CGCAGAG123450.030.3591968
GCAGAGT138400.026.735929
GAGTACT84500.025.62653412-13
CAGAGTA135100.024.70292310-11
AGAGTAC131500.024.1803610-11
CATGGGG62600.024.1446884
GTACTTT91850.023.6084714-15
Produced by FastQC (version 0.11.5)