FastQCFastQC Report
Thu 26 May 2016
SRR1364226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262360
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24590.10869180855389945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.054.332811
GTATCAA32800.047.3463021
ATCAACG38300.039.2965773
CAACGCA39550.037.9066855
TCAACGC40450.037.354954
GTACATG85250.037.2007941
AACGCAG41450.036.439246
TATCAAC44800.033.8621332
TACATGG90650.033.7324182
ACATGGG93500.031.5575313
ACGCAGA51400.029.0376267
CGCAGAG52600.027.696888
GAGTACA66400.025.7177661
CATGGGG57900.024.863934
AGTACAT65850.024.3927152
CATGGGA47850.022.0051484
GCAGAGT65700.021.6337139
GTATAGG7200.021.486231
TGGTATC13350.020.9444392
ATGGGAG24400.020.237185
Produced by FastQC (version 0.11.5)