##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364226_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2262360 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.159505560565073 33.0 31.0 34.0 28.0 34.0 2 31.185777241464667 33.0 31.0 34.0 28.0 34.0 3 31.262532930214466 34.0 31.0 34.0 28.0 34.0 4 34.62364919818243 37.0 35.0 37.0 32.0 37.0 5 34.70640967838894 37.0 35.0 37.0 32.0 37.0 6 34.804848034795526 37.0 35.0 37.0 32.0 37.0 7 34.72515028554253 37.0 35.0 37.0 32.0 37.0 8 34.714675825244434 37.0 35.0 37.0 32.0 37.0 9 36.25884872434095 39.0 37.0 39.0 32.0 39.0 10-11 36.210934599268015 39.0 37.0 39.0 32.0 39.0 12-13 36.17245398610301 39.0 37.0 39.0 32.0 39.0 14-15 37.511137705758586 40.0 38.0 41.0 32.0 41.0 16-17 37.547516973425985 40.0 38.0 41.0 32.0 41.0 18-19 37.43533743524461 40.0 38.0 41.0 31.0 41.0 20-21 37.40296902349759 40.0 38.0 41.0 31.0 41.0 22-23 37.44805115012642 40.0 38.0 41.0 31.0 41.0 24-25 37.303321973514386 40.0 38.0 41.0 30.0 41.0 26-27 37.31957668098799 40.0 37.5 41.0 30.0 41.0 28-29 37.40663554871904 40.0 38.0 41.0 30.5 41.0 30-31 37.302121899255646 40.0 37.5 41.0 30.0 41.0 32-33 37.11871386516735 40.0 37.0 41.0 30.0 41.0 34-35 37.07486275393836 40.0 37.0 41.0 30.0 41.0 36-37 37.0984144875263 40.0 37.0 41.0 30.0 41.0 38-39 36.94033199844411 40.0 37.0 41.0 30.0 41.0 40-41 36.9284813203911 40.0 37.0 41.0 30.0 41.0 42-43 36.85488030198553 40.0 37.0 41.0 30.0 41.0 44-45 36.842387816262665 40.0 37.0 41.0 30.0 41.0 46-47 36.7587910412136 40.0 36.0 41.0 30.0 41.0 48-49 36.660726851606285 40.0 36.0 41.0 30.0 41.0 50-51 36.52997732456373 40.0 35.5 41.0 30.0 41.0 52-53 36.367566390848495 39.5 35.0 41.0 30.0 41.0 54-55 36.16740328683322 39.0 35.0 41.0 29.0 41.0 56-57 35.95767030888099 39.0 35.0 41.0 29.0 41.0 58-59 35.716748881698756 39.0 35.0 41.0 29.0 41.0 60-61 35.459128078643545 38.0 35.0 40.0 29.0 41.0 62-63 35.15447939320002 37.5 34.0 40.0 28.0 41.0 64-65 34.826421745433976 37.0 34.0 40.0 28.0 41.0 66-67 34.50372840750367 36.5 34.0 39.5 28.0 41.0 68-69 34.26009742039287 36.0 34.0 39.0 28.0 41.0 70-71 34.07526719885429 36.0 34.0 39.0 28.0 41.0 72-73 33.75635575240015 35.0 34.0 38.5 28.0 40.5 74-75 33.43398530737814 35.0 34.0 37.0 27.5 39.5 76-77 33.11701409147969 35.0 34.0 37.0 27.0 39.0 78-79 32.79253677575629 35.0 33.0 36.0 27.0 39.0 80-81 32.52285246379887 35.0 33.0 36.0 26.0 37.5 82-83 32.2848395038809 35.0 33.0 35.5 26.0 37.0 84-85 32.06958949946074 35.0 33.0 35.0 26.0 37.0 86-87 31.88549589808872 35.0 33.0 35.0 26.0 36.0 88-89 31.741029279159815 35.0 33.0 35.0 25.0 36.0 90-91 31.616368968687567 35.0 33.0 35.0 25.0 36.0 92-93 31.510477333404054 35.0 33.0 35.0 25.0 35.5 94-95 31.396811736416836 35.0 33.0 35.0 25.0 35.0 96-97 31.32419928746972 35.0 33.0 35.0 25.0 35.0 98-99 31.25236301914815 35.0 33.0 35.0 24.0 35.0 100-101 31.1607878498559 35.0 33.0 35.0 24.0 35.0 102-103 30.18872062801676 34.0 30.5 35.0 21.5 35.0 104-105 30.888024894358104 34.5 32.0 35.0 23.0 35.0 106-107 31.00276127583585 35.0 32.0 35.0 23.0 35.0 108-109 30.972764723563003 35.0 32.0 35.0 23.0 35.0 110-111 30.910227152177374 35.0 32.0 35.0 23.0 35.0 112-113 31.07140441839495 35.0 33.0 35.0 23.0 35.0 114-115 31.069618893544792 35.0 33.0 35.0 23.0 35.0 116-117 30.986224340953694 35.0 33.0 35.0 21.5 35.0 118-119 30.751584849449248 35.0 33.0 35.0 19.0 35.0 120-121 30.70391317031772 35.0 33.0 35.0 18.5 35.0 122-123 30.571736151629274 35.0 33.0 35.0 8.5 35.0 124-125 29.697034954649126 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.42903679753388246 1101 2 0.4346088969496087 1101 3 0.434885187232009 1101 4 0.4778699910394053 1101 5 0.367963565826372 1101 6 0.476477176697081 1101 7 0.5537304623905328 1101 8 0.5152083183311476 1101 9 0.5716306676871312 1101 10-11 0.5182053947985921 1101 12-13 0.49873869352366995 1101 14-15 0.5929259540005418 1101 16-17 0.6234688086871145 1101 18-19 0.6759144068980802 1101 20-21 0.7569340100578259 1101 22-23 0.6979251347657112 1101 24-25 0.9551207492551654 1101 26-27 0.8912074344374972 1101 28-29 0.7707240688212735 1101 30-31 0.8232748369685723 1101 32-33 1.0900370266719506 1101 34-35 0.9847147183951961 1101 36-37 0.886259679164624 1101 38-39 0.9606503330178171 1101 40-41 0.885677562081689 1101 42-43 0.8310190028953528 1101 44-45 0.7764445934234558 1101 46-47 0.7912440454414309 1101 48-49 0.7306807497259982 1101 50-51 0.6554609911802629 1101 52-53 0.6128348182527077 1101 54-55 0.6840155485521748 1101 56-57 0.6808722288906992 1101 58-59 0.7465180697418177 1101 60-61 0.5932583735089025 1101 62-63 0.5584117203197323 1101 64-65 0.5159528910797135 1101 66-67 0.39190465763428506 1101 68-69 0.450082746187924 1101 70-71 0.40993631433612876 1101 72-73 0.38781652011797974 1101 74-75 0.36715874275643756 1101 76-77 0.48877632637622526 1101 78-79 0.457325751629611 1101 80-81 0.47459325369815275 1101 82-83 0.41614605970466556 1101 84-85 0.42225668431653673 1101 86-87 0.411588482028705 1101 88-89 0.3965851342966431 1101 90-91 0.41296164608678154 1101 92-93 0.3722668451796558 1101 94-95 0.43727825333741066 1101 96-97 0.3688044365143419 1101 98-99 0.3896060416842033 1101 100-101 0.3578121890672179 1101 102-103 0.34050739593769563 1101 104-105 0.38163078529824546 1101 106-107 0.3311989485014344 1101 108-109 0.3303535890562692 1101 110-111 0.24958693378024677 1101 112-113 0.3437961759987509 1101 114-115 0.3260592694039559 1101 116-117 0.3989968435626494 1101 118-119 0.24763091699914597 1101 120-121 0.21434931865656282 1101 122-123 0.2594069758837563 1101 124-125 0.14272630220745341 1102 1 0.26159034370110135 1102 2 0.29394727786235464 1102 3 0.24052533022154776 1102 4 0.3628996570324077 1102 5 0.34790784773338146 1102 6 0.38316760794126026 1102 7 0.39584632159862565 1102 8 0.40106300498555925 1102 9 0.472018565252462 1102 10-11 0.48345075324365183 1102 12-13 0.4791254978265229 1102 14-15 0.614572981671671 1102 16-17 0.6117227469449702 1102 18-19 0.6547404402153916 1102 20-21 0.7028547577191588 1102 22-23 0.693323667707034 1102 24-25 0.8441505879491729 1102 26-27 0.8404970483770384 1102 28-29 0.6758246743374272 1102 30-31 0.8111682953818544 1102 32-33 0.8562896608648884 1102 34-35 0.8705286709707778 1102 36-37 0.8276062913290048 1102 38-39 0.7998390824854269 1102 40-41 0.7986831636637177 1102 42-43 0.685562489707614 1102 44-45 0.5369809104634484 1102 46-47 0.6031731281281694 1102 48-49 0.5554369986256731 1102 50-51 0.5144911141990889 1102 52-53 0.4360619356804847 1102 54-55 0.4106523524960366 1102 56-57 0.41622133909280024 1102 58-59 0.4878023410551009 1102 60-61 0.505112268074555 1102 62-63 0.5122166901454008 1102 64-65 0.5365591175636908 1102 66-67 0.4983994312748621 1102 68-69 0.5104927732236675 1102 70-71 0.5027174888542305 1102 72-73 0.42078474973833124 1102 74-75 0.4306644640516666 1102 76-77 0.48788309647586203 1102 78-79 0.45660618005830145 1102 80-81 0.37192049501306457 1102 82-83 0.369225618290848 1102 84-85 0.39654625278325284 1102 86-87 0.3390540114726477 1102 88-89 0.31755068226477334 1102 90-91 0.3092453099788379 1102 92-93 0.34408623400764426 1102 94-95 0.27732131753958456 1102 96-97 0.2931608691816763 1102 98-99 0.2923477084537822 1102 100-101 0.2921500027662951 1102 102-103 0.16868439800331103 1102 104-105 0.3015854962782285 1102 106-107 0.27673758082626065 1102 108-109 0.18826388390302995 1102 110-111 0.17450632003303568 1102 112-113 0.29820406763058926 1102 114-115 0.32238099644465734 1102 116-117 0.3131657357651534 1102 118-119 0.15464822606281814 1102 120-121 0.27254553705977 1102 122-123 0.27354916835749066 1102 124-125 0.23467814811220578 1103 1 0.203398475821583 1103 2 -0.10117945256417471 1103 3 0.17014669157501316 1103 4 0.2549224201917184 1103 5 0.23485388044618105 1103 6 0.247848942494322 1103 7 0.30064443733753166 1103 8 0.3300831956097312 1103 9 0.39569246275310377 1103 10-11 0.35545004506712985 1103 12-13 0.382903261883051 1103 14-15 0.5027854993710861 1103 16-17 0.3777692695864445 1103 18-19 0.4627163981820033 1103 20-21 0.6232782169056819 1103 22-23 0.5593681521429374 1103 24-25 0.7063628462769316 1103 26-27 0.6555245481662197 1103 28-29 0.5724886513720477 1103 30-31 0.6087857729546045 1103 32-33 0.7606848313567909 1103 34-35 0.7176135474455307 1103 36-37 0.6337932243531696 1103 38-39 0.7486099845832186 1103 40-41 0.662233743733033 1103 42-43 0.7133368871281291 1103 44-45 0.5934540415188607 1103 46-47 0.66983558849806 1103 48-49 0.5415036155761115 1103 50-51 0.5108339339805568 1103 52-53 0.5049391461843697 1103 54-55 0.43784316370584264 1103 56-57 0.4729522931608372 1103 58-59 0.3946416097629353 1103 60-61 0.4233653380717257 1103 62-63 0.3721891204177936 1103 64-65 0.4838598140305166 1103 66-67 0.4619989782337228 1103 68-69 0.4561021587206753 1103 70-71 0.4391274735037456 1103 72-73 0.49828057241604284 1103 74-75 0.4853126018311684 1103 76-77 0.44100262004730695 1103 78-79 0.47323613904241313 1103 80-81 0.4825972052373757 1103 82-83 0.47166023485441855 1103 84-85 0.511368263485025 1103 86-87 0.46445720649974476 1103 88-89 0.4597371512974391 1103 90-91 0.5383989131466187 1103 92-93 0.5372017151380568 1103 94-95 0.5616437500699867 1103 96-97 0.6074318849793698 1103 98-99 0.4968636526165433 1103 100-101 0.5549029750494974 1103 102-103 0.5285181004117092 1103 104-105 0.5394377411618336 1103 106-107 0.5513427184630828 1103 108-109 0.5376624733848523 1103 110-111 0.4879392237046396 1103 112-113 0.5764457036942012 1103 114-115 0.638474757497427 1103 116-117 0.6642059299781664 1103 118-119 0.5447030717766559 1103 120-121 0.5086101058134425 1103 122-123 0.5665684441870553 1103 124-125 0.5020407620668799 1104 1 0.34352360645372926 1104 2 0.3365471874926662 1104 3 0.35820326891307985 1104 4 0.44560276621152184 1104 5 0.43873900702700297 1104 6 0.45835007885970214 1104 7 0.4604537796267394 1104 8 0.4905696766214618 1104 9 0.5848443551352318 1104 10-11 0.5424458356812565 1104 12-13 0.5557301645085815 1104 14-15 0.5153119902655803 1104 16-17 0.5652484566823617 1104 18-19 0.6995407748690923 1104 20-21 0.7277848916136165 1104 22-23 0.740043533757607 1104 24-25 0.9402776365212304 1104 26-27 0.868248349227855 1104 28-29 0.731208816985216 1104 30-31 0.7943636005554637 1104 32-33 0.939745438533464 1104 34-35 0.9293341089834328 1104 36-37 0.8506028766299636 1104 38-39 0.9591920706768988 1104 40-41 0.8264508179106045 1104 42-43 0.7422083174752956 1104 44-45 0.7543991737162941 1104 46-47 0.7126881111371262 1104 48-49 0.5960934651707461 1104 50-51 0.5375390958876807 1104 52-53 0.5263578304558649 1104 54-55 0.49474756169308165 1104 56-57 0.44917398185657476 1104 58-59 0.40459612445588533 1104 60-61 0.4394862270915638 1104 62-63 0.4880579143358901 1104 64-65 0.4887981311048506 1104 66-67 0.47745980086710915 1104 68-69 0.4199627088105231 1104 70-71 0.4357588522272451 1104 72-73 0.41425878274415595 1104 74-75 0.435585874302582 1104 76-77 0.41606722637074967 1104 78-79 0.46399118132809747 1104 80-81 0.41525163506739204 1104 82-83 0.43867533063598074 1104 84-85 0.48030601122572136 1104 86-87 0.4841673143969132 1104 88-89 0.45660869366982126 1104 90-91 0.47373216514656846 1104 92-93 0.48341771817041135 1104 94-95 0.4862643989261777 1104 96-97 0.4452592710013832 1104 98-99 0.3749364812839495 1104 100-101 0.43211432212970635 1104 102-103 0.4678739017065965 1104 104-105 0.4920857947620618 1104 106-107 0.5511052403501999 1104 108-109 0.5312896964331948 1104 110-111 0.484604363452263 1104 112-113 0.48497826543467326 1104 114-115 0.5657107347793264 1104 116-117 0.4896368817781678 1104 118-119 0.4292643472681448 1104 120-121 0.4056384067592127 1104 122-123 0.37626196314463556 1104 124-125 0.37052814541870305 1105 1 0.2948897585103296 1105 2 0.2397073594028818 1105 3 0.2584145989967155 1105 4 0.3637114994581694 1105 5 0.24521188455695864 1105 6 0.2987997327671579 1105 7 0.3909400863104153 1105 8 0.32258597804938205 1105 9 0.3768598728406545 1105 10-11 0.3385422961086064 1105 12-13 0.3503674144263442 1105 14-15 0.42456895602894207 1105 16-17 0.43749580328329074 1105 18-19 0.5960827884103068 1105 20-21 0.666229376046239 1105 22-23 0.5842053855151264 1105 24-25 0.7650560630470693 1105 26-27 0.7821523081607964 1105 28-29 0.5572957249352939 1105 30-31 0.6355522050928784 1105 32-33 0.8255800583060307 1105 34-35 0.7859047283957565 1105 36-37 0.6486222500438288 1105 38-39 0.7310557799598527 1105 40-41 0.6010761607462243 1105 42-43 0.5621180398622201 1105 44-45 0.5293207756863225 1105 46-47 0.4600077286392974 1105 48-49 0.39874389090737594 1105 50-51 0.4271339508869971 1105 52-53 0.430629779755904 1105 54-55 0.40747619468510976 1105 56-57 0.41282863625729505 1105 58-59 0.3615087888958115 1105 60-61 0.37069438344282446 1105 62-63 0.39346063254675556 1105 64-65 0.4253799426243958 1105 66-67 0.4658566260347783 1105 68-69 0.4780626268977741 1105 70-71 0.5638776724636472 1105 72-73 0.5490992694358283 1105 74-75 0.45847246175140555 1105 76-77 0.49046655291937924 1105 78-79 0.44852387647757297 1105 80-81 0.3942169787211114 1105 82-83 0.47950598367275177 1105 84-85 0.5180165979760716 1105 86-87 0.49466949661720605 1105 88-89 0.45175949665883763 1105 90-91 0.4379262732720939 1105 92-93 0.45324560514800183 1105 94-95 0.46765047403360427 1105 96-97 0.4709996567372201 1105 98-99 0.5533600166080035 1105 100-101 0.5288390409778536 1105 102-103 0.4800381427865119 1105 104-105 0.41898579761508614 1105 106-107 0.5211646348283274 1105 108-109 0.45882243489365493 1105 110-111 0.5006430896557141 1105 112-113 0.48980340079008045 1105 114-115 0.46581378741282364 1105 116-117 0.5203788693327915 1105 118-119 0.5246916702405287 1105 120-121 0.4779071166729203 1105 122-123 0.5256003707373935 1105 124-125 0.580923347799569 1106 1 0.10330814502202301 1106 2 0.23692770347921055 1106 3 0.22542362482926848 1106 4 0.21444719603564977 1106 5 0.20589960792216289 1106 6 0.21200792792261325 1106 7 0.23885319791521198 1106 8 0.18358171734126927 1106 9 0.275590246216062 1106 10-11 0.32337224341319626 1106 12-13 0.3173764402588972 1106 14-15 0.4005823498922396 1106 16-17 0.386565852759027 1106 18-19 0.3482319239265763 1106 20-21 0.3398398163909562 1106 22-23 0.42439663442848286 1106 24-25 0.5461554759923288 1106 26-27 0.560429136133358 1106 28-29 0.49000980673659456 1106 30-31 0.5308267900226369 1106 32-33 0.6702412944479903 1106 34-35 0.658716688428818 1106 36-37 0.5764263218641332 1106 38-39 0.5826124945997293 1106 40-41 0.4319088769744823 1106 42-43 0.4006672526507913 1106 44-45 0.34827824373100924 1106 46-47 0.26124086557223336 1106 48-49 0.22538029259448678 1106 50-51 0.3080434027903607 1106 52-53 0.25080535956636396 1106 54-55 0.25915633034090746 1106 56-57 0.2701485757273403 1106 58-59 0.26424229609180117 1106 60-61 0.3018361888239838 1106 62-63 0.2767580128015368 1106 64-65 0.3352235735633613 1106 66-67 0.20453677242282708 1106 68-69 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-2.0840803912290156 2216 70-71 -2.138649346006577 2216 72-73 -2.1412317493062822 2216 74-75 -2.2249281628662594 2216 76-77 -2.275547377557025 2216 78-79 -2.255229195632495 2216 80-81 -2.2276157697039274 2216 82-83 -2.2261983568118424 2216 84-85 -2.176259385725203 2216 86-87 -2.1953505011647962 2216 88-89 -2.1389657538500515 2216 90-91 -2.2268064755106955 2216 92-93 -2.2223895837934755 2216 94-95 -2.0679423582461816 2216 96-97 -2.185907197958681 2216 98-99 -2.1981016101883064 2216 100-101 -2.2495066523441736 2216 102-103 -2.2832883934473927 2216 104-105 -2.246807048258894 2216 106-107 -2.2802914469658226 2216 108-109 -2.203590141886739 2216 110-111 -2.2188225510066815 2216 112-113 -2.2542458459839807 2216 114-115 -2.286696452954075 2216 116-117 -2.168236454191252 2216 118-119 -2.025063669954296 2216 120-121 -1.9535841669414182 2216 122-123 -1.9073332216011991 2216 124-125 -1.8127080920049607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31945.0 3 6305.0 4 4575.0 5 3931.0 6 4341.0 7 4988.0 8 4940.0 9 5257.0 10 5862.0 11 6777.0 12 7492.0 13 7462.0 14 7284.0 15 6923.0 16 6653.0 17 5996.0 18 5564.0 19 5411.0 20 5401.0 21 5743.0 22 6714.0 23 8785.0 24 13950.0 25 20841.0 26 24798.0 27 23197.0 28 27005.0 29 33274.0 30 38939.0 31 47567.0 32 61987.0 33 83649.0 34 121110.0 35 197537.0 36 406368.0 37 729667.0 38 273275.0 39 847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.69090883357718 17.490946339702887 12.090373840538815 24.727770986181117 2 16.60967703418051 20.03299089433627 38.37128465481176 24.986047416671457 3 19.80269386682964 23.94476065056455 27.990027235834734 28.26251824677108 4 13.325055143709728 16.237324022138854 35.14511595656427 35.292504877587156 5 14.81850479043346 37.31275830443507 32.90523228715892 14.96350461797255 6 33.749965742313236 34.79732268442349 17.03464579320027 14.418065780063 7 29.74904469015694 31.740597739965654 20.018520523967283 18.491837045910124 8 26.45414522887604 34.89298785339203 19.474531020704045 19.178335897027882 9 26.564232858117197 16.76486149953654 18.631336859772123 38.03956878257414 10-11 25.627324945204727 26.34940477203464 26.742569292235345 21.280700990525286 12-13 26.068168466967567 23.667389085566192 26.788738221647197 23.47570422581904 14-15 23.064288972819284 25.348997786217968 24.22259030179578 27.36412293916697 16-17 23.756632069179062 28.34355079757221 24.689464673830162 23.210352459418566 18-19 23.662146965579307 27.014150551399407 26.307639671052286 23.016062811969 20-21 24.363414209339275 26.557569670569453 25.842938519441272 23.236077600650002 22-23 24.08490355004107 26.314963470412494 25.763350341865305 23.836782637681132 24-25 24.073212929622265 26.484808675149562 25.896043722301147 23.545934672927025 26-27 23.699146107750018 26.867168141397308 25.798774975187833 23.634910775664846 28-29 23.682625552090144 26.796123328699935 25.97338385645645 23.547867262753467 30-31 23.651514187049443 26.47190527467903 25.893531264200753 23.983049274070773 32-33 23.635418904427606 26.54594471304943 25.990714783941694 23.82792159858127 34-35 24.117769806245796 26.671708066268835 25.45533962469351 23.755182502791854 36-37 24.238083070143485 26.407409928947583 25.497061881739636 23.8574451191693 38-39 23.743048191175657 26.40642799554235 25.925159551432163 23.925364261849825 40-41 23.84164347968117 26.40498323663279 25.860143411938118 23.89322987174792 42-43 24.059436160076807 26.334174134458486 25.625036881281282 23.981352824183425 44-45 23.774882268645843 26.605805280694355 25.68848641724505 23.930826033414753 46-47 23.872606233853606 26.743700074503547 25.431589016263693 23.952104675379154 48-49 24.006391767615487 26.507159796400543 25.576331010460663 23.910117425523307 50-51 23.86918652772673 26.603193490931105 25.49411102449896 24.033508956843207 52-53 24.08408344693333 26.37524510193942 25.5257204538342 24.01495099729305 54-55 23.751940561357102 26.255441528543493 25.675107592855195 24.31751031724421 56-57 23.692256009088087 26.648845589836018 25.745496015674295 23.9134023854016 58-59 23.812025609804348 26.22018888413192 25.691738568164652 24.276046937899075 60-61 23.895511444806683 26.211904155090487 25.974526620921186 23.91805777918164 62-63 23.705513081192624 26.44513343561367 25.75015466935681 24.09919881383689 64-65 23.552814496719055 26.321782429866886 25.659073378700043 24.46632969471402 66-67 23.619305597702137 26.73110470802309 25.47071500764827 24.178874686626504 68-69 23.616186818039754 26.275862160426183 25.90446555624173 24.20348546529233 70-71 23.56265861825775 26.304921450879533 25.827931223410754 24.30448870745196 72-73 23.7794856879154 26.311727440056153 25.78458319114665 24.1242036808818 74-75 23.569184527065477 26.473459419737328 25.620779392995075 24.336576660202113 76-77 23.7895204449341 26.284926232922956 25.766042723132838 24.159510599010105 78-79 23.835946689392912 26.412076441162025 25.80386502343423 23.948111846010832 80-81 23.608659694107363 26.622565472172756 25.82240528100422 23.94636955271567 82-83 23.71849345419706 26.454237947423614 25.7030425524156 24.124226045963727 84-85 23.70347644916885 26.188991060104584 25.74794207347398 24.359590417252587 86-87 23.837816727252473 25.981066815606006 25.968269643272745 24.212846813868776 88-89 23.67047174919607 25.98859580299029 25.9660968251688 24.374835622644845 90-91 23.573249691470437 26.307957587351876 26.053774315488713 24.065018405688967 92-93 23.66580066105218 25.93707074982236 26.09388001825574 24.303248570869723 94-95 23.511289096341873 26.00107851977581 26.09018900617055 24.39744337771177 96-97 23.662164079583473 26.004926781206443 25.980615551495827 24.352293587714254 98-99 23.57351777668769 25.965355415484993 25.86762336929958 24.593503438527737 100-101 23.351021726286064 25.93776960026383 26.132351501163004 24.5788571722871 102-103 23.562773170071335 25.749924023494696 26.178840405134352 24.508462401299614 104-105 23.387706590859306 25.954692857074967 26.09772686229899 24.559873689766736 106-107 23.400920758361064 26.10403455231529 26.003000512023718 24.492044177299928 108-109 23.60070581880595 26.158812767505463 25.719245187609097 24.521236226079495 110-111 23.327361598073484 26.292078411403498 26.057562930862243 24.32299705966077 112-113 23.49532027729529 26.150103028612374 26.013144160923574 24.341432533168756 114-115 23.19657326037694 26.374222074835572 25.91959321284475 24.50961145194274 116-117 23.26271709264462 26.342512722289023 26.059343115502507 24.335427069563845 118-119 23.312684076574968 26.436667898464925 25.68723030383421 24.5634177211259 120-121 23.3849407622422 26.395907635919798 25.83740739052488 24.381744211313126 122-123 23.423719949078915 26.390454352839598 25.88086774511076 24.304957952970724 124-125 23.52585504642241 26.41478936415242 25.714894505805407 24.344461083619766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1719.0 1 1309.0 2 842.0 3 710.5 4 563.0 5 452.5 6 394.5 7 378.0 8 394.0 9 410.5 10 382.0 11 349.5 12 336.5 13 346.0 14 387.0 15 435.5 16 514.5 17 621.5 18 792.0 19 987.5 20 1286.5 21 1764.0 22 2282.5 23 2990.0 24 3910.0 25 5125.0 26 7033.0 27 9474.5 28 12245.0 29 15080.5 30 18008.0 31 21997.5 32 25804.0 33 28357.0 34 31130.5 35 33837.0 36 36977.0 37 41645.5 38 45760.0 39 48161.0 40 51443.0 41 58156.5 42 65707.0 43 72193.5 44 75437.0 45 77799.0 46 79581.5 47 81444.0 48 88063.5 49 91596.5 50 91123.0 51 91433.0 52 93129.0 53 95895.0 54 95295.0 55 90230.5 56 82978.5 57 76166.0 58 71204.0 59 64597.0 60 54898.5 61 44932.0 62 36928.5 63 29312.5 64 22655.5 65 17667.5 66 13439.5 67 11190.0 68 8625.5 69 5741.0 70 4387.5 71 3688.5 72 3152.5 73 2465.0 74 1787.0 75 1399.0 76 946.0 77 511.5 78 276.5 79 175.0 80 98.5 81 56.5 82 40.0 83 30.0 84 26.5 85 24.0 86 17.5 87 12.0 88 10.0 89 8.5 90 8.5 91 7.0 92 5.0 93 6.5 94 5.0 95 5.0 96 5.5 97 2.5 98 1.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06170547569794374 2 0.04994784207641578 3 0.04424583178627628 4 0.04402482363549568 5 0.04322919429268551 6 0.004154953234675295 7 2.2100815078060078E-4 8 0.0 9 9.282342332785232E-4 10-11 0.0033372230767870718 12-13 0.0023426863982743684 14-15 0.007425873866228186 16-17 0.0025194929188988493 18-19 0.005127389098109938 20-21 0.009459148853409713 22-23 0.015824183595891016 24-25 0.0036245336728018534 26-27 0.01613359500698386 28-29 0.007138563270213406 30-31 0.006895454304354744 32-33 0.002386888028430488 34-35 0.018653087925882706 36-37 0.01626619989745222 38-39 0.027780724553121517 40-41 0.005989320886154281 42-43 0.0014807546102300253 44-45 2.6520978093672097E-4 46-47 0.0022763839530401883 48-49 0.0033151222617090123 50-51 0.0017459643911667462 52-53 0.0015912586856203256 54-55 0.005657808659983381 56-57 0.0025415937339769087 58-59 0.007934192613023567 60-61 0.015536872999876236 62-63 0.012221750738167222 64-65 0.002431089658586609 66-67 0.003690836118036033 68-69 0.009856963524814794 70-71 0.002187980692727948 72-73 0.0033593238918651317 74-75 9.945366785127036E-4 76-77 1.1050407539030039E-4 78-79 0.003138315741084531 80-81 0.0027847026998355697 82-83 0.0014365529800739053 84-85 0.011183012429498401 86-87 0.0062545306670910026 88-89 0.0015470570554642054 90-91 7.072260824979226E-4 92-93 0.0025415937339769087 94-95 0.0 96-97 0.0013702505348397247 98-99 0.024907618592973708 100-101 0.01460863876659771 102-103 0.006519740448027723 104-105 0.01372460616347531 106-107 0.032974416096465634 108-109 0.0010166374935907637 110-111 0.0015912586856203256 112-113 0.016221998267296096 114-115 0.032488198164748315 116-117 0.051760108912816696 118-119 0.36258597217065364 120-121 0.12097986173730087 122-123 0.018940398521897488 124-125 0.32298131155077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2262360.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.39767972216356 #Duplication Level Percentage of deduplicated Percentage of total 1 85.62662858978494 67.98555632460399 2 9.62929177316496 15.290868483140319 3 2.573411656448147 6.129687434758572 4 0.972635630465756 3.0890004909633886 5 0.4668819241574433 1.853467074116007 6 0.24035657716773573 1.1450252719847624 7 0.1376028142958401 0.7647740924830622 8 0.0922831826651952 0.5861656464794468 9 0.06266969627561561 0.44782456258589354 >10 0.19518779140705722 2.3454347434856535 >50 0.0024991554214343938 0.13522030313212804 >100 4.5934951591847787E-4 0.04763062724314963 >500 0.0 0.0 >1k 9.185922996595247E-5 0.17934494502368348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2459 0.10869180855389945 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.4201630156120155E-5 0.0 2 0.0 0.0 0.0 4.4201630156120155E-5 0.0 3 0.0 0.0 0.0 4.4201630156120155E-5 0.0 4 0.0 0.0 0.0 4.4201630156120155E-5 0.0 5 4.4201630156120155E-5 0.0 0.0 4.4201630156120155E-5 0.0 6 1.3260489046836049E-4 0.0 0.0 4.4201630156120155E-5 0.0 7 2.6520978093672097E-4 0.0 0.0 4.4201630156120155E-5 0.0 8 2.6520978093672097E-4 0.0 0.0 4.4201630156120155E-5 0.0 9 2.6520978093672097E-4 0.0 0.0 4.4201630156120155E-5 0.0 10-11 2.6520978093672097E-4 0.0 0.0 4.4201630156120155E-5 0.0 12-13 2.87310596014781E-4 0.0 0.0 4.4201630156120155E-5 0.0 14-15 3.978146714050814E-4 0.0 0.0 4.4201630156120155E-5 0.0 16-17 4.862179317173217E-4 0.0 0.0 1.3260489046836049E-4 0.0 18-19 7.514277126540427E-4 0.0 0.0 1.989073357025407E-4 0.0 20-21 9.282342332785233E-4 0.0 0.0 2.4310896585866086E-4 0.0 22-23 0.0010166374935907637 0.0 0.0 4.641171166392617E-4 0.0 24-25 0.001370250534839725 0.0 0.0 9.503350483565833E-4 0.0 26-27 0.001370250534839725 0.0 0.0 0.0020995774324157074 0.0 28-29 0.0016354603157764458 0.0 0.0 0.0050831874679538175 0.0 30-31 0.0018122668364009265 0.0 0.0 0.01352569882777277 0.0 32-33 0.0018122668364009265 0.0 0.0 0.03220088756873354 0.0 34-35 0.002011174172103467 0.0 0.0 0.06168337488286568 0.0 36-37 0.0022763839530401883 0.0 0.0 0.10959794197209993 0.0 38-39 0.0024752912887427286 0.0 0.0 0.17152442582082428 0.0 40-41 0.0025415937339769087 0.0 0.0 0.23292049010767518 0.0 42-43 0.0026962994395233297 0.0 0.0 0.29482487314132144 0.0 44-45 0.0029173075903039304 0.0 0.0 0.3773272158277198 0.0 46-47 0.0032488198164748313 0.0 0.0 0.4757642461853993 0.0 48-49 0.003447727152177372 0.0 0.0 0.5737371594264397 0.0 50-51 0.003447727152177372 0.0 0.0 0.6791580473487863 0.0 52-53 0.003469827967255432 0.0 0.0 0.7817500309411411 0.0 54-55 0.0035361304124896125 0.0 0.0 0.9017353559999293 0.0 56-57 0.0035803320426457327 0.0 0.0 1.0426280521225624 0.0 58-59 0.0035803320426457327 0.0 0.0 1.198836613094291 0.0 60-61 0.0035803320426457327 0.0 0.0 1.3660292791598154 0.0 62-63 0.0035803320426457327 0.0 0.0 1.538415636768684 0.0 64-65 0.003602432857723793 0.0 0.0 1.7224491239236903 0.0 66-67 0.0038455418235824536 0.0 0.0 1.9320974557541681 0.0 68-69 0.0039339450838946945 0.0 0.0 2.1750517159072826 0.0 70-71 0.004066549974363055 0.0 0.0 2.448836613094291 0.0 72-73 0.004309658940221716 0.0 0.0 2.726555455365194 0.0 74-75 0.004464364645768136 0.0 0.0 3.0129599179617745 0.0 76-77 0.0044864654608461965 0.0 0.0 3.3315431673120104 0.0 78-79 0.004508566275924256 0.0 0.0 3.685686628122845 0.0 80-81 0.005370498063968599 0.0 0.0 4.083810710939019 0.0 82-83 0.005436800509202779 0.0 0.0 4.502820064003961 0.0 84-85 0.0055915062147492 0.0 0.0 4.920348662458672 0.0 86-87 0.005790413550451741 0.0 0.0 5.3554916105305965 0.0 88-89 0.005834615180607861 0.0 0.0 5.829885606181156 0.0 90-91 0.006387135557559363 0.0 0.0 6.354161141462897 0.0 92-93 0.006630244523418024 0.0 0.0 6.894548170936544 0.0 94-95 0.006630244523418024 0.0 0.0 7.447731572340388 0.0 96-97 0.006630244523418024 0.0 0.0 8.005622447355858 0.0 98-99 0.006983857564666985 0.0 0.0 8.595625806679749 0.0 100-101 0.007249067345603706 0.0 0.0 9.247025230290493 0.0 102-103 0.007514277126540427 0.0 0.0 9.937255785993388 0.0 104-105 0.008088898318569989 0.0 0.0 10.614225852649446 0.0 106-107 0.00850881380505313 0.0 0.0 11.292963984511749 0.0 108-109 0.00866351951059955 0.0 0.0 11.998156792022488 0.0 110-111 0.008751922770911792 0.0 0.0 12.756435757350731 0.0 112-113 0.008818225216145972 0.0 0.0 13.54402040347248 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2015 0.0 54.33281 1 GTATCAA 3280 0.0 47.346302 1 ATCAACG 3830 0.0 39.296577 3 CAACGCA 3955 0.0 37.906685 5 TCAACGC 4045 0.0 37.35495 4 GTACATG 8525 0.0 37.200794 1 AACGCAG 4145 0.0 36.43924 6 TATCAAC 4480 0.0 33.862133 2 TACATGG 9065 0.0 33.732418 2 ACATGGG 9350 0.0 31.557531 3 ACGCAGA 5140 0.0 29.037626 7 CGCAGAG 5260 0.0 27.69688 8 GAGTACA 6640 0.0 25.717766 1 CATGGGG 5790 0.0 24.86393 4 AGTACAT 6585 0.0 24.392715 2 CATGGGA 4785 0.0 22.005148 4 GCAGAGT 6570 0.0 21.633713 9 GTATAGG 720 0.0 21.48623 1 TGGTATC 1335 0.0 20.944439 2 ATGGGAG 2440 0.0 20.23718 5 >>END_MODULE