FastQCFastQC Report
Thu 26 May 2016
SRR1364226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262360
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67780.2995986491981824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57310.2533195424247246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31010.1370692551141286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37250.071.761521
GTATCAA69000.055.307051
ATCAACG88750.042.5751383
GTGGTAT17900.041.2420461
TCAACGC91950.041.028744
TATCAAC92350.040.9817162
CAACGCA93100.040.5147745
TGGTATC17550.039.67152
AACGCAG96300.039.3520936
ACGCAGA112350.033.622987
GTACATG83850.033.1577761
CGCAGAG115150.032.7537358
TACATGG90450.030.7898222
ACATGGG91300.029.9804213
GCAGAGT130150.028.7502739
GAGTACT81950.026.16928112-13
AATACCG1859.27359E-725.7271795
CAGAGTA125500.025.09907710-11
GTACTTT87550.024.56335314-15
CATGGGG54000.024.0222364
Produced by FastQC (version 0.11.5)