FastQCFastQC Report
Thu 26 May 2016
SRR1364225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026739
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28450.14037327944051997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.055.6201361
GTATCAA34700.046.307371
GTACATG96250.038.583331
ATCAACG40050.038.0326653
CAACGCA40950.037.0505685
TCAACGC41000.037.0062984
AACGCAG42050.036.485126
TACATGG100900.036.445012
ACATGGG98050.035.6213043
TATCAAC46500.033.5265662
CATGGGG50800.029.9844134
ACGCAGA52150.028.7340987
CATGGGA56600.028.698924
CGCAGAG52850.028.3528168
TAAGGTG8400.025.4995065
ATGGGGA30800.023.7609065
ATGGGAG25750.023.5684785
GTATAGG7050.022.7924211
GAGTACA73100.022.714471
GCAGAGT65450.022.2602089
Produced by FastQC (version 0.11.5)