##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364225_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2026739 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.096069104112566 33.0 31.0 34.0 28.0 34.0 2 31.108729836451563 33.0 31.0 34.0 27.0 34.0 3 31.168404515825667 34.0 31.0 34.0 27.0 34.0 4 34.51111514605482 37.0 35.0 37.0 32.0 37.0 5 34.62254883337223 37.0 35.0 37.0 32.0 37.0 6 34.625720924105174 37.0 35.0 37.0 31.0 37.0 7 34.56755457905532 37.0 35.0 37.0 31.0 37.0 8 34.53212426464384 37.0 35.0 37.0 31.0 37.0 9 36.00205058470775 39.0 37.0 39.0 31.0 39.0 10-11 35.983345906897725 39.0 37.0 39.0 31.0 39.0 12-13 35.94672649018941 39.0 37.0 39.0 31.0 39.0 14-15 37.271275186395485 40.0 38.0 41.0 31.0 41.0 16-17 37.31653434408673 40.0 38.0 41.0 31.0 41.0 18-19 37.215015352248116 40.0 38.0 41.0 30.5 41.0 20-21 37.2030885575301 40.0 38.0 41.0 30.0 41.0 22-23 37.25738933330833 40.0 38.0 41.0 30.5 41.0 24-25 37.11550845964872 40.0 37.0 41.0 30.0 41.0 26-27 37.1547809066683 40.0 37.0 41.0 30.0 41.0 28-29 37.25040249385836 40.0 37.0 41.0 30.0 41.0 30-31 37.14279268322167 40.0 37.0 41.0 30.0 41.0 32-33 36.95932530039635 40.0 37.0 41.0 30.0 41.0 34-35 36.91066437266959 40.0 37.0 41.0 30.0 41.0 36-37 36.93362292826062 40.0 37.0 41.0 30.0 41.0 38-39 36.76889130766221 40.0 37.0 41.0 29.5 41.0 40-41 36.76222345353793 40.0 37.0 41.0 30.0 41.0 42-43 36.69096711515395 40.0 36.0 41.0 30.0 41.0 44-45 36.686369335173396 40.0 36.0 41.0 30.0 41.0 46-47 36.60542057956155 40.0 36.0 41.0 30.0 41.0 48-49 36.5108825063316 40.0 36.0 41.0 30.0 41.0 50-51 36.3924155996406 40.0 35.5 41.0 29.5 41.0 52-53 36.24535867716564 40.0 35.0 41.0 29.0 41.0 54-55 36.05651369021862 39.0 35.0 41.0 29.0 41.0 56-57 35.8689323094883 39.0 35.0 41.0 29.0 41.0 58-59 35.645608289967285 39.0 35.0 41.0 29.0 41.0 60-61 35.408595778736185 38.5 35.0 40.5 28.0 41.0 62-63 35.11895537609924 38.0 34.0 40.0 28.0 41.0 64-65 34.817594668085036 37.0 34.0 40.0 28.0 41.0 66-67 34.51597122273761 37.0 34.0 40.0 28.0 41.0 68-69 34.28238021767973 36.0 34.0 39.0 27.5 41.0 70-71 34.105142300019885 36.0 34.0 39.0 28.0 41.0 72-73 33.79550573606173 35.5 34.0 39.0 27.5 41.0 74-75 33.46602818616506 35.0 34.0 37.5 27.0 40.0 76-77 33.13946245668535 35.0 34.0 37.0 27.0 39.0 78-79 32.80192096762336 35.0 33.0 37.0 26.0 39.0 80-81 32.51116892703007 35.0 33.0 36.0 26.0 38.5 82-83 32.243915965499255 35.0 33.0 36.0 26.0 37.0 84-85 32.009865108432805 35.0 33.0 35.5 26.0 37.0 86-87 31.809032144740886 35.0 33.0 35.0 25.0 36.5 88-89 31.64340598370091 35.0 33.0 35.0 25.0 36.0 90-91 31.50604641248824 35.0 33.0 35.0 25.0 36.0 92-93 31.38916629126888 35.0 33.0 35.0 25.0 36.0 94-95 31.271619581998472 35.0 33.0 35.0 24.0 35.5 96-97 31.189325068496736 35.0 33.0 35.0 24.0 35.0 98-99 31.122520709375998 35.0 33.0 35.0 24.0 35.0 100-101 31.031362449728356 35.0 32.0 35.0 23.5 35.0 102-103 30.057108734770488 34.0 30.5 35.0 20.0 35.0 104-105 30.75728522518193 34.5 32.0 35.0 21.5 35.0 106-107 30.875257248219924 35.0 32.0 35.0 23.0 35.0 108-109 30.842770085343993 35.0 32.0 35.0 20.0 35.0 110-111 30.783348275234257 35.0 32.0 35.0 20.0 35.0 112-113 30.938229342801417 35.0 33.0 35.0 23.0 35.0 114-115 30.934934641312964 35.0 33.0 35.0 21.5 35.0 116-117 30.85824741123549 35.0 33.0 35.0 19.5 35.0 118-119 30.62474694570934 35.0 33.0 35.0 17.0 35.0 120-121 30.58785961093165 35.0 33.0 35.0 16.0 35.0 122-123 30.46720668028789 35.0 33.0 35.0 2.0 35.0 124-125 29.621296082031282 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5817133350452579 1101 2 0.6481995515589354 1101 3 0.6494384320283473 1101 4 0.6286128364266261 1101 5 0.554906732879374 1101 6 0.536425676930719 1101 7 0.4951392690025429 1101 8 0.5493048388498849 1101 9 0.5910843706074971 1101 10-11 0.6425087988080591 1101 12-13 0.5571062449430215 1101 14-15 0.7868934656931046 1101 16-17 0.7998140119159345 1101 18-19 0.7784618591234818 1101 20-21 0.8414074661162871 1101 22-23 0.696519213291559 1101 24-25 0.9118684300366837 1101 26-27 0.8782397558381092 1101 28-29 0.814237370710508 1101 30-31 0.8190146538755059 1101 32-33 0.9211458842278475 1101 34-35 0.8758947747667492 1101 36-37 0.7831027396972203 1101 38-39 0.8732280816689197 1101 40-41 0.7694781198539289 1101 42-43 0.7456662023249621 1101 44-45 0.6483735198033926 1101 46-47 0.6762252004401645 1101 48-49 0.6210305583072326 1101 50-51 0.5738684795718285 1101 52-53 0.5223629914896151 1101 54-55 0.5366116033268042 1101 56-57 0.48677224334894476 1101 58-59 0.5297106781505079 1101 60-61 0.44168689296562746 1101 62-63 0.41711068410776164 1101 64-65 0.31178149273830513 1101 66-67 0.25222196334455305 1101 68-69 0.37203486680081 1101 70-71 0.26861898707466025 1101 72-73 0.20850268837979513 1101 74-75 0.3148695784999447 1101 76-77 0.36876033919769213 1101 78-79 0.2813687521843278 1101 80-81 0.3002354125314852 1101 82-83 0.3027840436897762 1101 84-85 0.3030467986876886 1101 86-87 0.2778558055793212 1101 88-89 0.32238132684310017 1101 90-91 0.3148601796149997 1101 92-93 0.29716993914883005 1101 94-95 0.37392948639670465 1101 96-97 0.3491421969922044 1101 98-99 0.32342512177427807 1101 100-101 0.2696803756930599 1101 102-103 0.24545276455423348 1101 104-105 0.31961498636779595 1101 106-107 0.34448628826399386 1101 108-109 0.1998867646477933 1101 110-111 0.31928440643266853 1101 112-113 0.4199890176351211 1101 114-115 0.40253562135637466 1101 116-117 0.3869637557293544 1101 118-119 0.3398322106613243 1101 120-121 0.2725391984011232 1101 122-123 0.24356658948243037 1101 124-125 0.14396856971991312 1102 1 0.017807008119472556 1102 2 0.1476277759644411 1102 3 0.10173648174268024 1102 4 0.1942116679296717 1102 5 0.04792765339780658 1102 6 0.10055973774323235 1102 7 0.14072407522248653 1102 8 0.18603377474682503 1102 9 0.22193603920367622 1102 10-11 0.22339350468352137 1102 12-13 0.24293660833845365 1102 14-15 0.252784641114836 1102 16-17 0.20881003743482296 1102 18-19 0.37498203407583475 1102 20-21 0.42629087881476124 1102 22-23 0.4076389610414992 1102 24-25 0.5535079236326723 1102 26-27 0.5173426283361735 1102 28-29 0.37742174704307274 1102 30-31 0.46298195148463606 1102 32-33 0.596590008022325 1102 34-35 0.5280308966563609 1102 36-37 0.47519828687730836 1102 38-39 0.5313989684910396 1102 40-41 0.44055563553839505 1102 42-43 0.43149949477359684 1102 44-45 0.30425840264248194 1102 46-47 0.34355181940720314 1102 48-49 0.17639582710766888 1102 50-51 0.12166635634997647 1102 52-53 0.16639826754189357 1102 54-55 0.1625639422612224 1102 56-57 0.14668237755572733 1102 58-59 0.18632422399641513 1102 60-61 0.19204503074194434 1102 62-63 0.2456385395257925 1102 64-65 0.29466569237573736 1102 66-67 0.28177562201271655 1102 68-69 0.22925401880572593 1102 70-71 0.28810292695389705 1102 72-73 0.11368423231791525 1102 74-75 0.20132293930780065 1102 76-77 0.16065562456924454 1102 78-79 0.16382127040120764 1102 80-81 0.2407383480530143 1102 82-83 0.17630227838846224 1102 84-85 0.20354979034940612 1102 86-87 0.150294989281484 1102 88-89 0.10185443654810555 1102 90-91 0.17448640313544317 1102 92-93 0.1456173700884662 1102 94-95 0.17955428151553576 1102 96-97 0.15642422600593875 1102 98-99 0.15515071000749003 1102 100-101 0.1898943698793758 1102 102-103 0.018647875866797392 1102 104-105 0.1502542619934104 1102 106-107 0.10402188199650553 1102 108-109 0.08518888550199577 1102 110-111 0.05903363554248031 1102 112-113 0.16954740184245054 1102 114-115 0.2114850139073532 1102 116-117 0.2020319404266644 1102 118-119 0.1080840565140413 1102 120-121 0.24239442018292934 1102 122-123 0.27450099162728137 1102 124-125 0.19469934408591527 1103 1 0.1300794925476758 1103 2 -0.1350011164480236 1103 3 0.04462309103562134 1103 4 0.07217020178853772 1103 5 0.11879943792663994 1103 6 -0.013377743348542026 1103 7 0.10812361219893063 1103 8 0.11832355282594165 1103 9 0.21109425036215868 1103 10-11 0.13891852576418273 1103 12-13 0.14472111571614477 1103 14-15 0.16201514516968274 1103 16-17 0.22461570486031945 1103 18-19 0.28710285933629365 1103 20-21 0.39192260844447446 1103 22-23 0.23720548974517186 1103 24-25 0.44708581407903836 1103 26-27 0.4236044962650283 1103 28-29 0.3835694682934587 1103 30-31 0.38866055563382673 1103 32-33 0.5631822576345087 1103 34-35 0.5095210400620687 1103 36-37 0.41465638338846844 1103 38-39 0.5498095904259444 1103 40-41 0.4935650025750462 1103 42-43 0.47784476272862975 1103 44-45 0.46163438077438457 1103 46-47 0.3624187419741247 1103 48-49 0.26029274178588224 1103 50-51 0.31405250111743044 1103 52-53 0.3192648797099622 1103 54-55 0.23893100250506194 1103 56-57 0.21942424371087554 1103 58-59 0.2330381800013086 1103 60-61 0.22670099222387563 1103 62-63 0.1931038896435524 1103 64-65 0.24398020438081147 1103 66-67 0.21970979599771567 1103 68-69 0.28694100372420905 1103 70-71 0.2642962639633737 1103 72-73 0.1882893856542367 1103 74-75 0.27951430807380717 1103 76-77 0.26178772217181034 1103 78-79 0.3000761793878297 1103 80-81 0.29795454944323296 1103 82-83 0.18169456461912858 1103 84-85 0.15744304317929192 1103 86-87 0.18648345574661107 1103 88-89 0.23557358708511345 1103 90-91 0.15111994867002565 1103 92-93 0.1673350987984321 1103 94-95 0.16264308634402624 1103 96-97 0.18639649036564165 1103 98-99 0.20441168893684392 1103 100-101 0.1958203776565135 1103 102-103 0.18320790693529077 1103 104-105 0.3046678620408976 1103 106-107 0.3040474863266418 1103 108-109 0.21107082236228436 1103 110-111 0.18096531775412217 1103 112-113 0.2619121619878051 1103 114-115 0.24361576620977843 1103 116-117 0.2632153825784016 1103 118-119 0.2700643440406587 1103 120-121 0.17574824413241785 1103 122-123 0.181195200828963 1103 124-125 0.16355414393228784 1104 1 0.39287798059525514 1104 2 0.4487432449068862 1104 3 0.415089061355598 1104 4 0.3139112978537 1104 5 0.31432120341921177 1104 6 0.25514288545624453 1104 7 0.19870458436083283 1104 8 0.4058220656713729 1104 9 0.4999763544816105 1104 10-11 0.40898543696923184 1104 12-13 0.3953862142410287 1104 14-15 0.4446236071150125 1104 16-17 0.34871534923328085 1104 18-19 0.6011800494670254 1104 20-21 0.615985020200668 1104 22-23 0.6570111276018906 1104 24-25 0.8036309425906936 1104 26-27 0.7772041628557318 1104 28-29 0.5538538624159344 1104 30-31 0.6962197085505792 1104 32-33 0.8309352996718857 1104 34-35 0.7501150561821106 1104 36-37 0.7106223601587587 1104 38-39 0.8149070760955013 1104 40-41 0.775458734451469 1104 42-43 0.6429578008098176 1104 44-45 0.5633760000312833 1104 46-47 0.5696177168050198 1104 48-49 0.5744829914988472 1104 50-51 0.5708260422367175 1104 52-53 0.6456768523434491 1104 54-55 0.5896599232536985 1104 56-57 0.6080336789361596 1104 58-59 0.6303917257361533 1104 60-61 0.6314494588420914 1104 62-63 0.6106361519389125 1104 64-65 0.5739056244750955 1104 66-67 0.6201261634417108 1104 68-69 0.5596752968635172 1104 70-71 0.4807338729915145 1104 72-73 0.46813460690699316 1104 74-75 0.4585060817797668 1104 76-77 0.4644931609289955 1104 78-79 0.5403564596465813 1104 80-81 0.42661635401033493 1104 82-83 0.47167698394368074 1104 84-85 0.44796334660182424 1104 86-87 0.394686707849047 1104 88-89 0.3881595876212103 1104 90-91 0.4149771832648952 1104 92-93 0.3967613008135089 1104 94-95 0.34178644231077726 1104 96-97 0.3446046518699113 1104 98-99 0.28599483843636975 1104 100-101 0.372051598298647 1104 102-103 0.41789935282221435 1104 104-105 0.4456060379795268 1104 106-107 0.5302829355046548 1104 108-109 0.48049574864959155 1104 110-111 0.3762270999601576 1104 112-113 0.4614877674926454 1104 114-115 0.4773362614214989 1104 116-117 0.4577690641997485 1104 118-119 0.4536177404150088 1104 120-121 0.4462200914005727 1104 122-123 0.502365222372994 1104 124-125 0.5595604709686874 1105 1 0.20197449327680062 1105 2 0.17336098060694738 1105 3 0.24384701128841968 1105 4 0.23508659547021438 1105 5 0.2257279189998016 1105 6 0.05706564008843884 1105 7 0.010901535336124368 1105 8 0.03931489475385064 1105 9 0.12280654476870723 1105 10-11 0.11245258766217603 1105 12-13 0.10064005818800581 1105 14-15 0.08396934788257937 1105 16-17 0.11496033924881743 1105 18-19 0.2808439153712996 1105 20-21 0.257922389358761 1105 22-23 0.311952442255901 1105 24-25 0.4531230558596562 1105 26-27 0.43472396844987315 1105 28-29 0.2971136402296892 1105 30-31 0.3346348207373069 1105 32-33 0.5530640211210098 1105 34-35 0.5372812584221549 1105 36-37 0.3744328740958096 1105 38-39 0.4666620264288426 1105 40-41 0.3424480142919393 1105 42-43 0.32676457945017745 1105 44-45 0.25018805921655485 1105 46-47 0.08989128823156278 1105 48-49 0.03991658417255195 1105 50-51 -0.019467299277337702 1105 52-53 -0.008873889460915052 1105 54-55 0.09384863953305711 1105 56-57 0.06271551119150587 1105 58-59 0.060042099123791104 1105 60-61 0.00839167110351724 1105 62-63 -0.00852229849526509 1105 64-65 -0.03741899979382168 1105 66-67 0.04495544759978998 1105 68-69 0.04263531356583172 1105 70-71 0.08372864357612286 1105 72-73 0.05402228667703213 1105 74-75 0.02473942562306064 1105 76-77 0.027976516844532284 1105 78-79 0.10311690072405355 1105 80-81 0.03735156834251541 1105 82-83 0.027756498894810022 1105 84-85 -0.0035910623265280606 1105 86-87 0.011297913171070206 1105 88-89 0.04042263772531385 1105 90-91 0.014530296911654972 1105 92-93 0.048071708230697396 1105 94-95 0.04873892308749106 1105 96-97 0.023050995681959563 1105 98-99 0.04597503994355634 1105 100-101 0.10584033957466943 1105 102-103 -0.08204419164770016 1105 104-105 -0.03213770078262357 1105 106-107 0.05032180815518217 1105 108-109 0.03906498798410851 1105 110-111 -0.03228689106945026 1105 112-113 -0.04155905580083541 1105 114-115 -0.030550469611576148 1105 116-117 0.007039119050382681 1105 118-119 -0.0122103308368402 1105 120-121 -0.05202659157073697 1105 122-123 -0.12117971622895496 1105 124-125 -0.15087449936198283 1106 1 0.2550203760777876 1106 2 0.3006124832645405 1106 3 0.34928472758004503 1106 4 0.26185360489183296 1106 5 0.2266074911356455 1106 6 0.2691647244194044 1106 7 0.12327647945216569 1106 8 0.2547103876089878 1106 9 0.25557590545620457 1106 10-11 0.3356531241481946 1106 12-13 0.31787864544149613 1106 14-15 0.43717352717488467 1106 16-17 0.42854254248430124 1106 18-19 0.44696482342355637 1106 20-21 0.5033856052943833 1106 22-23 0.4521335890775475 1106 24-25 0.6847861625110951 1106 26-27 0.6456429491264402 1106 28-29 0.6162039321509383 1106 30-31 0.6488300089213723 1106 32-33 0.7384836889633135 1106 34-35 0.7726156514809475 1106 36-37 0.6653367868009283 1106 38-39 0.6246048343701531 1106 40-41 0.5726467128166064 1106 42-43 0.4749983151480208 1106 44-45 0.5114127044569017 1106 46-47 0.45340245674447033 1106 48-49 0.42577189853017927 1106 50-51 0.420247071593451 1106 52-53 0.5019664397163126 1106 54-55 0.4925549465553942 1106 56-57 0.46954080551369515 1106 58-59 0.3946600184190743 1106 60-61 0.42006173220331533 1106 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0.5263500250737962 2203 56-57 0.51408252138161 2203 58-59 0.5490756433766606 2203 60-61 0.378236467873883 2203 62-63 0.46745871534654526 2203 64-65 0.5392914488267024 2203 66-67 0.5059485941316382 2203 68-69 0.43660319622158283 2203 70-71 0.4733376547477235 2203 72-73 0.5229661136255359 2203 74-75 0.5551764971396338 2203 76-77 0.6012436842325926 2203 78-79 0.4806685907437398 2203 80-81 0.5346519456402774 2203 82-83 0.5406988660670748 2203 84-85 0.47423161566020156 2203 86-87 0.5934352613424352 2203 88-89 0.5835793131982356 2203 90-91 0.6141339019243119 2203 92-93 0.6071657289282371 2203 94-95 0.5741785276785301 2203 96-97 0.6506158327548377 2203 98-99 0.517750810151199 2203 100-101 0.5255282784000741 2203 102-103 0.08871234192772093 2203 104-105 0.10791502343413129 2203 106-107 0.47285767070954066 2203 108-109 0.5675734094650373 2203 110-111 0.648320582123592 2203 112-113 0.5168495047441759 2203 114-115 0.6804707277449609 2203 116-117 0.620844261588239 2203 118-119 0.7456264462310749 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118-119 0.729716708150324 2204 120-121 0.6024080820528148 2204 122-123 0.7270820842510339 2204 124-125 0.7429759003637422 2205 1 0.26400659167668294 2205 2 0.2700983235060477 2205 3 0.22490087273953208 2205 4 0.18702235598995287 2205 5 0.2141028191341121 2205 6 0.27366581854626304 2205 7 0.38249126374146414 2205 8 0.3449779295771336 2205 9 0.36941844702503346 2205 10-11 0.4411964340017249 2205 12-13 0.43505707596843024 2205 14-15 0.4630581726618104 2205 16-17 0.34471277903931963 2205 18-19 0.3826515677173248 2205 20-21 0.5876255465150138 2205 22-23 0.6032901690127019 2205 24-25 0.781214357441371 2205 26-27 0.8415121011381927 2205 28-29 0.653381404318587 2205 30-31 0.6431467786116585 2205 32-33 0.8133323480589425 2205 34-35 0.7905976662716228 2205 36-37 0.6060472504870944 2205 38-39 0.7833513004202786 2205 40-41 0.6936458023752792 2205 42-43 0.6875988230784458 2205 44-45 0.6438202627171563 2205 46-47 0.46561802369385674 2205 48-49 0.47259110405033056 2205 50-51 0.47916492422973533 2205 52-53 0.6152753348877837 2205 54-55 0.5638939342085294 2205 56-57 0.5312056353250583 2205 58-59 0.5774283989516036 2205 60-61 0.5778154977566885 2205 62-63 0.5729487647959104 2205 64-65 0.5907351888299601 2205 66-67 0.4457636427883642 2205 68-69 0.5865078616047228 2205 70-71 0.6126063332809224 2205 72-73 0.5862087150798274 2205 74-75 0.5213826713680163 2205 76-77 0.4534907167786457 2205 78-79 0.36549524464081884 2205 80-81 0.44917199816177344 2205 82-83 0.42105025940654883 2205 84-85 0.42544161092627064 2205 86-87 0.43124997763995054 2205 88-89 0.5019214966889578 2205 90-91 0.5253944835748001 2205 92-93 0.5444635391839263 2205 94-95 0.5580889524970161 2205 96-97 0.5323103290392979 2205 98-99 0.47118580823248024 2205 100-101 0.4841615823239884 2205 102-103 0.5489042356427412 2205 104-105 0.5588767879117107 2205 106-107 0.5323123064099526 2205 108-109 0.6062424201387948 2205 110-111 0.582338914358175 2205 112-113 0.22061188758481975 2205 114-115 0.4882232797498851 2205 116-117 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0.3496333486886556 2208 46-47 0.4021963231641408 2208 48-49 0.3175772396848586 2208 50-51 0.2979145615594945 2208 52-53 0.34866089273678824 2208 54-55 0.33510485907984133 2208 56-57 0.37060208986071785 2208 58-59 0.3513832869175886 2208 60-61 0.4240279769290538 2208 62-63 0.41612579761446256 2208 64-65 0.41017224170935407 2208 66-67 0.24773791599719175 2208 68-69 0.27148015359779976 2208 70-71 0.2013862483953659 2208 72-73 0.2140950521517766 2208 74-75 0.28075552125154246 2208 76-77 0.20073307583463418 2208 78-79 0.18791220351608473 2208 80-81 0.22992280847072522 2208 82-83 0.2822879597041492 2208 84-85 0.23821589325366688 2208 86-87 0.1460324169393168 2208 88-89 0.15964834174793197 2208 90-91 0.0960010961932376 2208 92-93 0.2021412683293491 2208 94-95 0.21955399522993702 2208 96-97 0.22875450268995934 2208 98-99 0.308967447584898 2208 100-101 0.22011049348428458 2208 102-103 0.25745454940431856 2208 104-105 0.2725800241737595 2208 106-107 0.3521876721888013 2208 108-109 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124-125 -2.0275075097757416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32576.0 3 6153.0 4 4633.0 5 3903.0 6 4360.0 7 4746.0 8 4756.0 9 4933.0 10 5640.0 11 6629.0 12 7180.0 13 7233.0 14 7115.0 15 6898.0 16 6210.0 17 5595.0 18 5019.0 19 4973.0 20 4879.0 21 4965.0 22 5876.0 23 8007.0 24 13099.0 25 19417.0 26 23185.0 27 21209.0 28 23496.0 29 28899.0 30 34066.0 31 41914.0 32 54675.0 33 73360.0 34 107510.0 35 170573.0 36 339877.0 37 639453.0 38 282674.0 39 1053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.78827289194904 18.49480192410518 12.308704043178318 24.40822114076746 2 16.092838056178515 20.57050304558229 38.81709277832436 24.519566119914835 3 18.94989058789324 25.22056424363976 28.95375567607698 26.875789492390023 4 12.724791134476076 16.57063171794207 36.24508533560402 34.45949181197784 5 13.746228113432284 38.66869248999058 33.579650545476625 14.005428851100504 6 32.507141105481125 36.44803461086218 17.17506798174522 13.869756301911481 7 28.628635543235742 33.068061494252305 20.610235424028705 17.69306753848325 8 26.189904077436708 35.84201024404228 19.608888959061822 18.359196719459188 9 26.552376629175782 16.832016341715537 19.178424198764997 37.43718283034369 10-11 24.973941249946648 27.256379367045607 27.23207842616745 20.537600956840297 12-13 25.244845405333926 24.78762137419345 27.28290061563527 22.68463260483735 14-15 22.463922762848618 26.273179644915757 24.656349990957715 26.606547601277907 16-17 22.9763778867079 29.326043584056006 25.282635678077686 22.414942851158408 18-19 22.817840030651652 28.059139133676382 26.81878839628756 22.30423243938441 20-21 23.56913297518789 27.39047771311948 26.540215370844354 22.50017394084827 22-23 23.387737306459265 27.299646740585402 26.40355662674147 22.909059326213864 24-25 23.171885832142955 27.399644243128492 26.634150192557726 22.79431973217083 26-27 22.98496856525301 27.679651799725626 26.405236821586836 22.93014281343453 28-29 22.799084868729288 27.72972096786012 26.627428427344498 22.843765736066096 30-31 22.927996664376305 27.332993685259726 26.656074252167723 23.08293539819625 32-33 22.764867679337677 27.578414323083518 26.613193505754346 23.043524491824463 34-35 23.299110708533927 27.52253578225498 26.31548688713936 22.86286662207173 36-37 23.480779536861306 27.32398390473117 26.188738443985837 23.00649811442169 38-39 22.958718454604117 27.383865192440332 26.52462370089048 23.132792652065064 40-41 23.07763285959768 27.252337324785447 26.64568759105359 23.02434222456328 42-43 23.17675342889884 27.260001751609447 26.38126123281233 23.18198358667938 44-45 22.932368759878887 27.430271419503356 26.503805261027164 23.133554559590593 46-47 23.15697776400602 27.635483742839007 26.117615828556044 23.089922664598927 48-49 23.109259142757402 27.56224797050337 26.18563186701354 23.142861019725682 50-51 22.890583599529876 27.452659449015098 26.345168347726204 23.311588603728826 52-53 23.26947685814399 27.315227932608888 26.310000979421538 23.10529422982558 54-55 22.86923757859137 27.183337593295644 26.466405674072423 23.481019154040567 56-57 22.893398447956205 27.60247350041262 26.29449852159872 23.209629530032455 58-59 22.975127783108327 27.223889920869095 26.364880511660417 23.436101784362165 60-61 22.999886992992085 27.21297577060663 26.705677935949208 23.081459300452078 62-63 22.862162774321625 27.37714156286285 26.623551257612267 23.13714440520326 64-65 22.7320734570644 27.311112925791708 26.432656750780403 23.524156866363494 66-67 22.774688403580274 27.606603969092003 26.185670018651376 23.433037608676344 68-69 22.787283324845944 27.287746183635527 26.575323606390413 23.34964688512812 70-71 22.70608833980408 27.245100436155482 26.60950601363655 23.439305210403884 72-73 22.87264395674396 27.261035493331075 26.444106891257686 23.422213658667282 74-75 22.71731933105642 27.479994750132974 26.34695601460095 23.45572990420966 76-77 22.839560691143216 27.315344083989523 26.426448726001357 23.418646498865904 78-79 22.90871492444411 27.325942422540134 26.4850345034992 23.280308149516564 80-81 22.907651031547786 27.547389521078113 26.445616307398158 23.099343139975943 82-83 23.001998562692723 27.424419865431922 26.44364144487621 23.12994012699915 84-85 22.9719457147545 27.336132059061914 26.29239569853209 23.399526527651503 86-87 22.992798336141792 27.005153914285646 26.58694423953242 23.41510351004014 88-89 22.93256946450647 27.067714247862472 26.554515578399474 23.44520070923158 90-91 22.885802027506433 27.26947303187505 26.592987902656795 23.251737037961718 92-93 22.949899170718012 26.945595608214962 26.572179407537305 23.532325813529724 94-95 22.858246671130324 26.86663156923511 26.63268432689162 23.642437432742945 96-97 22.932370522597022 26.858028178743332 26.624619764593266 23.58498153406638 98-99 22.766634036997456 26.85596151952759 26.490117701229522 23.887286742245436 100-101 22.526941772905225 26.904515697004644 26.734341547930907 23.834200982159224 102-103 22.74322860024514 26.72933940981303 26.728105823907093 23.799326166034742 104-105 22.63155855925431 26.88889936142479 26.7366391071741 23.74290297214681 106-107 22.653293851909122 27.057122783395986 26.59843378943937 23.691149575255523 108-109 22.74371900455369 27.08931079893561 26.263785279485113 23.903184917025584 110-111 22.542721574027908 27.254670588644537 26.537000940199047 23.66560689712851 112-113 22.62056771449798 27.201939772214445 26.657803761158515 23.51968875212906 114-115 22.44890404762416 27.35161314920751 26.439843355348376 23.759639447819954 116-117 22.524347664258187 27.26765622169271 26.5920575942153 23.6159385198338 118-119 22.43495583010218 27.370679783900375 26.266190945771335 23.92817344022611 120-121 22.495862992180438 27.42159443986149 26.475936198694928 23.606606369263144 122-123 22.759716586453347 27.358610913370846 26.386674677717643 23.494997822458167 124-125 22.69992657069297 27.374059581435628 26.24945521987833 23.676558627993074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2073.0 1 1724.5 2 1240.5 3 946.0 4 708.0 5 561.5 6 487.5 7 485.5 8 494.0 9 507.0 10 475.5 11 432.5 12 430.5 13 451.5 14 478.0 15 552.5 16 676.0 17 795.0 18 964.5 19 1189.5 20 1543.0 21 2090.0 22 2862.5 23 3776.5 24 5030.5 25 6909.0 26 9342.0 27 11832.5 28 14235.5 29 17075.5 30 20804.5 31 25683.5 32 31193.0 33 34679.0 34 37744.0 35 41279.5 36 45060.0 37 49422.5 38 51553.0 39 52761.0 40 54489.5 41 59601.0 42 64950.0 43 68889.5 44 71756.0 45 73091.5 46 73129.0 47 72023.5 48 74341.5 49 75799.0 50 75071.0 51 73684.0 52 72555.5 53 73462.5 54 72678.5 55 68095.0 56 62053.0 57 56974.5 58 53129.5 59 47785.5 60 40533.0 61 33698.5 62 28115.5 63 22427.5 64 17473.0 65 14252.5 66 11208.5 67 9452.5 68 7254.5 69 4542.5 70 3310.0 71 2612.5 72 2203.5 73 1833.0 74 1411.0 75 1194.5 76 848.5 77 472.5 78 274.5 79 156.5 80 97.5 81 63.0 82 42.0 83 30.0 84 21.5 85 18.0 86 16.5 87 18.5 88 18.0 89 13.0 90 11.0 91 8.5 92 7.0 93 6.5 94 4.5 95 3.0 96 2.0 97 3.0 98 3.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06305695997363252 2 0.05022847046412982 3 0.0449983939717941 4 0.04509707466032874 5 0.04420894846351701 6 0.004539311672593264 7 6.414244754751352E-4 8 0.0 9 0.0011841682624156341 10-11 0.003379813582311289 12-13 0.002565697901900541 14-15 0.007376381467964054 16-17 0.0028370697953707903 18-19 0.00466266253326156 20-21 0.009275984722255801 22-23 0.01601094171474472 24-25 0.0036511854757815387 26-27 0.01573956982127447 28-29 0.007129679746627465 30-31 0.006488255271152328 32-33 0.0025903680740342 34-35 0.017293790665694992 36-37 0.015048805001532018 38-39 0.029184813634118647 40-41 0.0057481501071425575 42-43 0.0015542208444205199 44-45 2.713718934702495E-4 46-47 0.0023436663526976093 48-49 0.0032811328937766534 50-51 0.002022954114960042 52-53 0.001603561188687838 54-55 0.005772820279276216 56-57 0.0027877294511034724 58-59 0.007919125254904555 60-61 0.015591548788472517 62-63 0.011841682624156342 64-65 0.002886410139638108 66-67 0.003897887197118129 68-69 0.010016089886265572 70-71 0.0026397084183015177 72-73 0.0030837715167073803 74-75 0.00103614722961368 76-77 4.934034426731809E-4 78-79 0.0029850908281727445 80-81 0.002541027729766882 82-83 0.001603561188687838 84-85 0.010928886255210957 86-87 0.006118202689147444 88-89 0.0018996032542917462 90-91 6.414244754751352E-4 92-93 0.002269655836296632 94-95 0.0 96-97 0.001677571705088815 98-99 0.022918589912169253 100-101 0.014086668288319315 102-103 0.006266223721949397 104-105 0.013543924501378816 106-107 0.0315038098146826 108-109 0.0014802103280195428 110-111 0.0019982839428263825 112-113 0.015566878616338856 114-115 0.030813044994940145 116-117 0.048353537381971734 118-119 0.35562053130669513 120-121 0.11491366179858384 122-123 0.018329937895308672 124-125 0.31718440312245433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2026739.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.75930552917322 #Duplication Level Percentage of deduplicated Percentage of total 1 85.35054621420964 62.95397015295708 2 9.217437205252063 13.59743534036311 3 2.66006150790189 5.886128684631861 4 1.091793054232961 3.2211958984719264 5 0.524235422083829 1.9333620333348112 6 0.29138149395638513 1.2895257982965571 7 0.18268674828419013 0.9432393377977318 8 0.11726845068664507 0.6919711586503229 9 0.0901244304685183 0.5982763862313152 >10 0.4316257928651257 5.73833530206683 >50 0.030721999271860003 1.550447477443375 >100 0.01198304009136943 1.3135753602732363 >500 0.0 0.0 >1k 1.3464069554972464E-4 0.28253706948182156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2845 0.14037327944051997 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4670172133659044E-5 0.0 16-17 0.0 0.0 0.0 4.934034426731809E-5 0.0 18-19 2.4670172133659044E-5 0.0 0.0 7.401051640097713E-5 0.0 20-21 4.934034426731809E-5 0.0 0.0 1.726912049356133E-4 0.0 22-23 4.934034426731809E-5 0.0 0.0 4.687332705395218E-4 0.0 24-25 4.934034426731809E-5 0.0 0.0 9.127963689453847E-4 0.0 26-27 4.934034426731809E-5 0.0 0.0 0.0018009225657571104 0.0 28-29 1.726912049356133E-4 0.0 0.0 0.005156065975934741 0.0 30-31 1.9736137706927235E-4 0.0 0.0 0.014234689321121268 0.0 32-33 1.9736137706927235E-4 0.0 0.0 0.030911725683474785 0.0 34-35 1.9736137706927235E-4 0.0 0.0 0.05775287296489583 0.0 36-37 2.4670172133659044E-4 0.0 0.0 0.09757053078862152 0.0 38-39 2.4670172133659044E-4 0.0 0.0 0.1565569123602003 0.0 40-41 2.4670172133659044E-4 0.0 0.0 0.21682614288272933 0.0 42-43 2.4670172133659044E-4 0.0 0.0 0.27694735237245643 0.0 44-45 2.4670172133659044E-4 0.0 0.0 0.35349889650320043 0.0 46-47 2.4670172133659044E-4 0.0 0.0 0.44458117202066966 0.0 48-49 2.4670172133659044E-4 0.0 0.0 0.5397586961123262 0.0 50-51 2.4670172133659044E-4 0.0 0.0 0.6404376685897888 0.0 52-53 2.4670172133659044E-4 0.0 0.0 0.7405985674524446 0.0 54-55 2.4670172133659044E-4 0.0 0.0 0.8513182999883063 0.0 56-57 2.4670172133659044E-4 0.0 0.0 0.9847592610592681 0.0 58-59 2.4670172133659044E-4 0.0 0.0 1.1355926934844596 0.0 60-61 2.4670172133659044E-4 0.0 0.0 1.3041886498458855 0.0 62-63 2.4670172133659044E-4 0.0 0.0 1.4726612553466432 0.0 64-65 2.4670172133659044E-4 0.0 0.0 1.6489789755859041 0.0 66-67 2.4670172133659044E-4 0.0 0.0 1.8523352044836559 0.0 68-69 2.4670172133659044E-4 0.0 0.0 2.092696691581896 0.0 70-71 3.453824098712266E-4 0.0 0.0 2.3563221510021766 0.0 72-73 3.7005258200488564E-4 0.0 0.0 2.622414627635823 0.0 74-75 4.440630984058628E-4 0.0 0.0 2.8937865211060725 0.0 76-77 4.934034426731809E-4 0.0 0.0 3.1979450733419545 0.0 78-79 4.934034426731809E-4 0.0 0.0 3.541674581680226 0.0 80-81 5.920841312078171E-4 0.0 0.0 3.916611857767576 0.0 82-83 6.414244754751352E-4 0.0 0.0 4.3156518920295115 0.0 84-85 6.414244754751352E-4 0.0 0.0 4.709042456872838 0.0 86-87 6.414244754751352E-4 0.0 0.0 5.125080239734865 0.0 88-89 6.414244754751352E-4 0.0 0.0 5.58594372536375 0.0 90-91 6.907648197424532E-4 0.0 0.0 6.086575528472093 0.0 92-93 6.907648197424532E-4 0.0 0.0 6.612074865091164 0.0 94-95 6.907648197424532E-4 0.0 0.0 7.137130138611829 0.0 96-97 6.907648197424532E-4 0.0 0.0 7.668081583272439 0.0 98-99 6.907648197424532E-4 0.0 0.0 8.235791584412201 0.0 100-101 6.907648197424532E-4 0.0 0.0 8.864510921238502 0.0 102-103 6.907648197424532E-4 0.0 0.0 9.530728919707965 0.0 104-105 8.387858525444076E-4 0.0 0.0 10.194183858898457 0.0 106-107 8.387858525444076E-4 0.0 0.0 10.856479299998668 0.0 108-109 8.387858525444076E-4 0.0 0.0 11.539473015519018 0.0 110-111 8.387858525444076E-4 0.0 0.0 12.272744541847766 0.0 112-113 8.387858525444076E-4 0.0 0.0 13.046302459270779 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2140 0.0 55.620136 1 GTATCAA 3470 0.0 46.30737 1 GTACATG 9625 0.0 38.58333 1 ATCAACG 4005 0.0 38.032665 3 CAACGCA 4095 0.0 37.050568 5 TCAACGC 4100 0.0 37.006298 4 AACGCAG 4205 0.0 36.48512 6 TACATGG 10090 0.0 36.44501 2 ACATGGG 9805 0.0 35.621304 3 TATCAAC 4650 0.0 33.526566 2 CATGGGG 5080 0.0 29.984413 4 ACGCAGA 5215 0.0 28.734098 7 CATGGGA 5660 0.0 28.69892 4 CGCAGAG 5285 0.0 28.352816 8 TAAGGTG 840 0.0 25.499506 5 ATGGGGA 3080 0.0 23.760906 5 ATGGGAG 2575 0.0 23.568478 5 GTATAGG 705 0.0 22.792421 1 GAGTACA 7310 0.0 22.71447 1 GCAGAGT 6545 0.0 22.260208 9 >>END_MODULE