FastQCFastQC Report
Thu 26 May 2016
SRR1364225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026739
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77420.38199294531757666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73090.3606285762498279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40950.20204870977466757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.075.250991
GTATCAA76300.054.945341
ATCAACG98950.042.108713
CAACGCA101600.040.992195
TCAACGC101800.040.9298324
TATCAAC103750.040.3326642
AACGCAG105100.039.965766
TGGTATC17450.038.2043042
GTGGTAT19300.037.643091
GTACATG89250.035.296381
ACGCAGA122550.034.1268277
CGCAGAG124000.033.7277648
TACATGG94700.032.6845932
ACATGGG92850.032.5024263
GCAGAGT133700.031.1028189
CATGGGG49850.028.1797144
GAGTACT89200.027.91227512-13
CAGAGTA133900.026.96882810-11
AGAGTAC130100.025.69880710-11
GTACTTT97250.025.57121314-15
Produced by FastQC (version 0.11.5)