FastQCFastQC Report
Thu 26 May 2016
SRR1364224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007326
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32970.16424835826368014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.051.396321
GTATCAA35800.041.3870661
ATCAACG35900.038.2806473
GTACATG93300.038.1388741
TCAACGC36050.038.120424
TACATGG92600.037.2658652
CAACGCA37100.036.7235765
ACATGGG93300.034.560453
AACGCAG39150.034.4812286
TATCAAC44600.032.1496622
CATGGGG35950.029.29044
ACGCAGA47300.028.7906937
CGCAGAG47550.027.7631938
CATGGGA63450.027.284274
CGTCGCA450.00955619826.43054468-69
GAGTACA63500.025.6758731
AGTACAT65550.024.1436652
GCAGAGT59950.021.0298069
ATGGGAT28250.020.6391125
ATGGGCG2055.94332E-520.3155045
Produced by FastQC (version 0.11.5)