##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2007326 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.055997381591233 31.0 31.0 34.0 28.0 34.0 2 31.097691157290843 33.0 31.0 34.0 27.0 34.0 3 31.184391573665664 34.0 31.0 34.0 27.0 34.0 4 34.53337175924588 37.0 35.0 37.0 32.0 37.0 5 34.65998547321163 37.0 35.0 37.0 32.0 37.0 6 34.72197889132109 37.0 35.0 37.0 32.0 37.0 7 34.62935915740642 37.0 35.0 37.0 31.0 37.0 8 34.59267004960829 37.0 35.0 37.0 31.0 37.0 9 36.06917710426707 39.0 37.0 39.0 31.0 39.0 10-11 36.051671975553546 39.0 37.0 39.0 31.0 39.0 12-13 36.00278156114154 39.0 37.0 39.0 31.0 39.0 14-15 37.32870420649162 40.0 38.0 41.0 31.0 41.0 16-17 37.37155449588158 40.0 38.0 41.0 31.0 41.0 18-19 37.2891461078071 40.0 38.0 41.0 31.0 41.0 20-21 37.27383992435708 40.0 38.0 41.0 30.5 41.0 22-23 37.32342379862564 40.0 38.0 41.0 30.5 41.0 24-25 37.19629422425655 40.0 38.0 41.0 30.0 41.0 26-27 37.22587810848861 40.0 37.5 41.0 30.0 41.0 28-29 37.321648302268784 40.0 38.0 41.0 30.0 41.0 30-31 37.2274677855017 40.0 37.0 41.0 30.0 41.0 32-33 37.0469041899522 40.0 37.0 41.0 30.0 41.0 34-35 37.0037156396121 40.0 37.0 41.0 30.0 41.0 36-37 37.030660689892926 40.0 37.0 41.0 30.0 41.0 38-39 36.87768528878718 40.0 37.0 41.0 30.0 41.0 40-41 36.88480545760878 40.0 37.0 41.0 30.0 41.0 42-43 36.82095982416409 40.0 37.0 41.0 30.0 41.0 44-45 36.82392820099974 40.0 37.0 41.0 30.0 41.0 46-47 36.76084054109796 40.0 36.5 41.0 30.0 41.0 48-49 36.68830723061426 40.0 36.0 41.0 30.0 41.0 50-51 36.592036370773855 40.0 36.0 41.0 30.0 41.0 52-53 36.464208603883975 40.0 36.0 41.0 30.0 41.0 54-55 36.30573758323261 40.0 35.0 41.0 29.5 41.0 56-57 36.14076238737505 39.0 35.0 41.0 29.0 41.0 58-59 35.93377458369991 39.0 35.0 41.0 29.0 41.0 60-61 35.71664991137463 39.0 35.0 41.0 29.0 41.0 62-63 35.44659138575398 38.5 35.0 40.5 28.0 41.0 64-65 35.16428298143899 38.0 34.0 40.0 28.0 41.0 66-67 34.87070959076901 37.0 34.0 40.0 28.0 41.0 68-69 34.65108333175578 37.0 34.0 40.0 28.0 41.0 70-71 34.484880632244085 36.5 34.0 39.0 28.0 41.0 72-73 34.165744876517316 36.0 34.0 39.0 28.0 41.0 74-75 33.82143458511473 36.0 34.0 39.0 28.0 40.5 76-77 33.468796050068605 35.0 34.0 37.5 27.0 39.5 78-79 33.11081483525845 35.0 34.0 37.0 27.0 39.0 80-81 32.78979871729854 35.0 33.0 36.5 26.0 39.0 82-83 32.494101107642706 35.0 33.0 36.0 26.0 38.0 84-85 32.227902941525194 35.0 33.0 36.0 26.0 37.0 86-87 32.00576612867068 35.0 33.0 35.5 26.0 37.0 88-89 31.817141560464023 35.0 33.0 35.0 25.5 36.0 90-91 31.65829765568722 35.0 33.0 35.0 25.0 36.0 92-93 31.521176928909405 35.0 33.0 35.0 25.0 36.0 94-95 31.39036110726409 35.0 33.0 35.0 25.0 36.0 96-97 31.30001653941612 35.0 33.0 35.0 25.0 35.5 98-99 31.224973173266328 35.0 33.0 35.0 24.0 35.0 100-101 31.130305939344183 35.0 33.0 35.0 24.0 35.0 102-103 30.177651263422085 34.0 30.5 35.0 20.0 35.0 104-105 30.858919029594595 35.0 32.0 35.0 23.0 35.0 106-107 30.96634926265091 35.0 32.0 35.0 23.0 35.0 108-109 30.942860800886354 35.0 32.0 35.0 23.0 35.0 110-111 30.87915415831808 35.0 32.0 35.0 22.0 35.0 112-113 31.019223334924174 35.0 33.0 35.0 23.0 35.0 114-115 31.00705440969728 35.0 33.0 35.0 23.0 35.0 116-117 30.929679334597367 35.0 33.0 35.0 21.5 35.0 118-119 30.70665452447684 35.0 33.0 35.0 18.0 35.0 120-121 30.66510920498215 35.0 33.0 35.0 17.5 35.0 122-123 30.54328893263974 35.0 33.0 35.0 4.5 35.0 124-125 29.703117729755903 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49961935426410164 1101 2 0.48957334497386995 1101 3 0.547143873917733 1101 4 0.5323710423032466 1101 5 0.3814039088965515 1101 6 0.4204210200538441 1101 7 0.477135842778857 1101 8 0.47749997886032247 1101 9 0.537488386737067 1101 10-11 0.5269491116974763 1101 12-13 0.5389519977168504 1101 14-15 0.7065246782621415 1101 16-17 0.6257946159522945 1101 18-19 0.70046170125363 1101 20-21 0.7736407740058766 1101 22-23 0.7874059960572808 1101 24-25 0.9385557993096825 1101 26-27 0.9201630770139744 1101 28-29 0.7805398076923851 1101 30-31 0.8588005354847823 1101 32-33 0.9504866865275332 1101 34-35 0.8862986939797466 1101 36-37 0.8033520804534149 1101 38-39 0.9105885519851924 1101 40-41 0.8216990734345444 1101 42-43 0.7617170954631547 1101 44-45 0.7659294722186445 1101 46-47 0.7667661758149507 1101 48-49 0.6453438109243237 1101 50-51 0.5496572376969056 1101 52-53 0.4747312407426847 1101 54-55 0.5532074931273598 1101 56-57 0.6179577422907343 1101 58-59 0.5542061250192276 1101 60-61 0.4429032451872743 1101 62-63 0.48117902458322703 1101 64-65 0.43752811559453875 1101 66-67 0.49342556738765353 1101 68-69 0.45292493955702895 1101 70-71 0.458788431070829 1101 72-73 0.4185584780514233 1101 74-75 0.3416819137341278 1101 76-77 0.43437019759301876 1101 78-79 0.4460956118833721 1101 80-81 0.5038744407742257 1101 82-83 0.4787840184552081 1101 84-85 0.47418622872254446 1101 86-87 0.4276639560257607 1101 88-89 0.4501828933410579 1101 90-91 0.4381055998610073 1101 92-93 0.510907725304854 1101 94-95 0.5069822294807267 1101 96-97 0.45908754563138743 1101 98-99 0.4674608076934419 1101 100-101 0.32621030152347785 1101 102-103 0.23685103567423837 1101 104-105 0.42824403598884686 1101 106-107 0.3622434696336789 1101 108-109 0.3733764879143102 1101 110-111 0.3126403285422725 1101 112-113 0.4282149700208109 1101 114-115 0.47401356865720956 1101 116-117 0.42659192824019243 1101 118-119 0.2964096806797265 1101 120-121 0.325555583226798 1101 122-123 0.3501288477568032 1101 124-125 0.27343741531535315 1102 1 -0.09917832584437747 1102 2 -0.006889581960848545 1102 3 -0.08365499512287045 1102 4 -0.16464802974014248 1102 5 -0.0861255933240983 1102 6 -0.11607342340801807 1102 7 -0.08286973341105863 1102 8 -0.08970600608641632 1102 9 -0.05593834163615696 1102 10-11 -0.06495411315808042 1102 12-13 -0.006428915009628611 1102 14-15 0.056180895099785744 1102 16-17 0.01647187737980005 1102 18-19 0.19523162814904538 1102 20-21 0.15000479112256926 1102 22-23 0.11597917925109158 1102 24-25 0.3383407966566949 1102 26-27 0.4415395975697294 1102 28-29 0.27844640282660293 1102 30-31 0.3349092638187088 1102 32-33 0.4906850588696301 1102 34-35 0.4167534113872904 1102 36-37 0.2484338563839259 1102 38-39 0.3365786327539766 1102 40-41 0.29856052599517113 1102 42-43 0.33620123648426414 1102 44-45 0.19162379383952555 1102 46-47 0.14586676340289273 1102 48-49 0.1061992033701884 1102 50-51 0.114371316716678 1102 52-53 0.08513034363934935 1102 54-55 0.027453663488557822 1102 56-57 0.017247699253360338 1102 58-59 0.0010746767819895808 1102 60-61 -0.007201461903086681 1102 62-63 0.0034815734178863522 1102 64-65 -0.023784119106565527 1102 66-67 -0.073908031244585 1102 68-69 0.011861152366059002 1102 70-71 0.003459607818854238 1102 72-73 0.04716795560909759 1102 74-75 0.10595173991421802 1102 76-77 0.10254378659588781 1102 78-79 0.14105266048220955 1102 80-81 0.11192687661119294 1102 82-83 0.04313607501300254 1102 84-85 0.06121569163966001 1102 86-87 0.07457666286065034 1102 88-89 0.15582307433386688 1102 90-91 0.06367144803142466 1102 92-93 0.08920602935705091 1102 94-95 0.0647209739291057 1102 96-97 -0.008809199052809191 1102 98-99 0.030517935390648177 1102 100-101 0.0036980314491934507 1102 102-103 -0.04256987577488047 1102 104-105 0.023633792012116572 1102 106-107 0.020032080450015144 1102 108-109 -0.0021854913137637766 1102 110-111 -6.146679204057648E-4 1102 112-113 0.05442459791453658 1102 114-115 0.08997031511006526 1102 116-117 0.07730798963232388 1102 118-119 -0.026752694531531063 1102 120-121 0.010587689307055115 1102 122-123 0.07335877592958084 1102 124-125 0.04286282210700776 1103 1 0.25761866146917 1103 2 0.01652141834415133 1103 3 0.24608288439920045 1103 4 0.2888274669158477 1103 5 0.2357764110644851 1103 6 0.22096571239633533 1103 7 0.30622392199312287 1103 8 0.23498228195575166 1103 9 0.3548617513639911 1103 10-11 0.3735522957264408 1103 12-13 0.3515791868545861 1103 14-15 0.46512770676571336 1103 16-17 0.3577843630585775 1103 18-19 0.5087292897617175 1103 20-21 0.5721936220057273 1103 22-23 0.596548532723574 1103 24-25 0.7513755100324531 1103 26-27 0.7831007278264792 1103 28-29 0.5869243607218309 1103 30-31 0.6403866824138618 1103 32-33 0.8236328120600902 1103 34-35 0.7701819038353435 1103 36-37 0.6504976003795164 1103 38-39 0.770411747890634 1103 40-41 0.7079194646987332 1103 42-43 0.6782649972842165 1103 44-45 0.6594583198226118 1103 46-47 0.6106076058945007 1103 48-49 0.5453850487179395 1103 50-51 0.5189228868504756 1103 52-53 0.4664651839240719 1103 54-55 0.49156506250110965 1103 56-57 0.49840148451446 1103 58-59 0.5522922790176423 1103 60-61 0.4797740153678731 1103 62-63 0.5259587912008783 1103 64-65 0.4433587272334947 1103 66-67 0.5497193972711543 1103 68-69 0.4430873104238273 1103 70-71 0.40976753411347033 1103 72-73 0.36093484714616864 1103 74-75 0.43028443560194773 1103 76-77 0.48115848040416864 1103 78-79 0.39960689218726486 1103 80-81 0.4626197985768883 1103 82-83 0.42125600032000676 1103 84-85 0.4506562971537136 1103 86-87 0.5309856013901637 1103 88-89 0.43286690815838114 1103 90-91 0.3864201523249058 1103 92-93 0.46586733583156814 1103 94-95 0.5135207762137526 1103 96-97 0.46391083579464265 1103 98-99 0.46619256880644144 1103 100-101 0.44109673263986693 1103 102-103 0.37642588580431635 1103 104-105 0.4297922794944853 1103 106-107 0.5361582087280503 1103 108-109 0.43344281006866936 1103 110-111 0.4957258816471253 1103 112-113 0.5162786906595045 1103 114-115 0.42276700906742093 1103 116-117 0.5124388818276451 1103 118-119 0.47962678234620526 1103 120-121 0.47558185759244154 1103 122-123 0.4955196995646709 1103 124-125 0.4219920895603515 1104 1 0.2703318892781077 1104 2 0.33016041313446465 1104 3 0.2445499196693639 1104 4 0.29569387143489934 1104 5 0.17101583700112855 1104 6 0.2897575046476817 1104 7 0.27837825641098135 1104 8 0.31646414786126087 1104 9 0.285106267222595 1104 10-11 0.3451668988123231 1104 12-13 0.32075142582142746 1104 14-15 0.360563543808361 1104 16-17 0.38223515049944723 1104 18-19 0.45367948836847205 1104 20-21 0.48136107045779397 1104 22-23 0.49913101939995386 1104 24-25 0.7330174589735492 1104 26-27 0.6297747133351734 1104 28-29 0.41849265549817005 1104 30-31 0.5498667119553673 1104 32-33 0.7075510539411667 1104 34-35 0.6505348050908637 1104 36-37 0.5254044847484636 1104 38-39 0.6099985659908 1104 40-41 0.5540517228276371 1104 42-43 0.44216527473486167 1104 44-45 0.3624216524240609 1104 46-47 0.34923232894286116 1104 48-49 0.2565844738561651 1104 50-51 0.33374833246814717 1104 52-53 0.33998042374921056 1104 54-55 0.4614115664233438 1104 56-57 0.32907994117029205 1104 58-59 0.3833404037307133 1104 60-61 0.2669645780537522 1104 62-63 0.31764844568500905 1104 64-65 0.3097261197962169 1104 66-67 0.21090105758373312 1104 68-69 0.3009084246177238 1104 70-71 0.2543101397543026 1104 72-73 0.19560300419522036 1104 74-75 0.19277989459314426 1104 76-77 0.19706219105707135 1104 78-79 0.21509742094033157 1104 80-81 0.18763786280970152 1104 82-83 0.18289874768023395 1104 84-85 0.2673077273219846 1104 86-87 0.28921390934042535 1104 88-89 0.3003001902598257 1104 90-91 0.2660041759581091 1104 92-93 0.28334353296125414 1104 94-95 0.36623001192703697 1104 96-97 0.30317309651840674 1104 98-99 0.26504484130688866 1104 100-101 0.3165385578259965 1104 102-103 0.2940394110243787 1104 104-105 0.4064025910775513 1104 106-107 0.29727439867195926 1104 108-109 0.25711394122897246 1104 110-111 0.29516658904647386 1104 112-113 0.3758319751562027 1104 114-115 0.3538618412995973 1104 116-117 0.3140277358965875 1104 118-119 0.23915331980204257 1104 120-121 0.25666611077114965 1104 122-123 0.33165575018444216 1104 124-125 0.31938804556745737 1105 1 0.2880015714510762 1105 2 0.2600647011336541 1105 3 0.3188069739513004 1105 4 0.3955948590202638 1105 5 0.30380970782236716 1105 6 0.3749600851763617 1105 7 0.4006598860819821 1105 8 0.39108780195532233 1105 9 0.40410260291699274 1105 10-11 0.36609186094219126 1105 12-13 0.476707431587279 1105 14-15 0.4265660092456969 1105 16-17 0.44412343854376957 1105 18-19 0.5541814897732635 1105 20-21 0.6597198903200763 1105 22-23 0.5886102592328442 1105 24-25 0.7960011459025012 1105 26-27 0.7297914128649339 1105 28-29 0.5977058029928202 1105 30-31 0.63823469963382 1105 32-33 0.7571557659165862 1105 34-35 0.7132068426873701 1105 36-37 0.6207792791778175 1105 38-39 0.6517317627941921 1105 40-41 0.6473741111989 1105 42-43 0.5105589887159212 1105 44-45 0.3789004775895748 1105 46-47 0.44066891163233635 1105 48-49 0.45113224664100926 1105 50-51 0.3201910787509519 1105 52-53 0.38952962107164524 1105 54-55 0.36819417070372396 1105 56-57 0.44119855296546007 1105 58-59 0.3704453202189555 1105 60-61 0.3391053054423949 1105 62-63 0.3749835730894375 1105 64-65 0.31961365184831436 1105 66-67 0.3596327677904725 1105 68-69 0.2682032418396787 1105 70-71 0.28398774292044493 1105 72-73 0.37549400591312576 1105 74-75 0.3034869447848223 1105 76-77 0.29766010192332715 1105 78-79 0.31950688664000637 1105 80-81 0.26600204231836955 1105 82-83 0.3355176119429544 1105 84-85 0.28187022503003334 1105 86-87 0.2619036337699683 1105 88-89 0.3081564163353576 1105 90-91 0.2183915599147106 1105 92-93 0.24143600660730868 1105 94-95 0.2597673503266478 1105 96-97 0.22578882961930447 1105 98-99 0.22978457405799446 1105 100-101 0.2452503527151535 1105 102-103 0.2091215123442609 1105 104-105 0.16735164340744646 1105 106-107 0.27607885902609297 1105 108-109 0.250609305134855 1105 110-111 0.1597880710733257 1105 112-113 0.2238683981846279 1105 114-115 0.23987039980860203 1105 116-117 0.27337566439326366 1105 118-119 0.2309446546642846 1105 120-121 0.2770399320015535 1105 122-123 0.15051121180514215 1105 124-125 0.09793913569131973 1106 1 0.28999580916225653 1106 2 0.3387132037948639 1106 3 0.3118784223604507 1106 4 0.30599090974585863 1106 5 0.21514508520865405 1106 6 0.26377799548222924 1106 7 0.17723721415508464 1106 8 0.33811433654972944 1106 9 0.3693149574882284 1106 10-11 0.38215239330109796 1106 12-13 0.36892205779875553 1106 14-15 0.42767627056734625 1106 16-17 0.486348858058129 1106 18-19 0.5027956003806864 1106 20-21 0.4351316068003612 1106 22-23 0.46242889723198033 1106 24-25 0.6883320076834352 1106 26-27 0.5905789690820882 1106 28-29 0.5438858040281929 1106 30-31 0.5928906179290081 1106 32-33 0.6795157777550358 1106 34-35 0.5984494510656404 1106 36-37 0.5657648539203848 1106 38-39 0.593278294953187 1106 40-41 0.43368901712459973 1106 42-43 0.38913384295749864 1106 44-45 0.4227732188508284 1106 46-47 0.3490682827230245 1106 48-49 0.3095122288572938 1106 50-51 0.3100922589815269 1106 52-53 0.32782660392837215 1106 54-55 0.25619289699631054 1106 56-57 0.2542463375057338 1106 58-59 0.3001763602277663 1106 60-61 0.3066552322009599 1106 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-0.044123294146345415 2209 96-97 -0.07444933176324753 2209 98-99 -0.11748491389020899 2209 100-101 -0.055045685066563266 2209 102-103 0.0589375618555934 2209 104-105 0.05184916050992072 2209 106-107 0.095389223145812 2209 108-109 0.05630472865505354 2209 110-111 0.044492978606132993 2209 112-113 0.07340374716002174 2209 114-115 0.05308444988326144 2209 116-117 0.11630297549434232 2209 118-119 0.10403859191855247 2209 120-121 -0.030498854831950695 2209 122-123 -0.0035808425369374675 2209 124-125 -0.06589448123636643 2210 1 -0.09548481240256379 2210 2 -0.1136210994705742 2210 3 -0.07157140386004457 2210 4 -0.08835812436342394 2210 5 -0.04231136888122222 2210 6 -0.047808146612823066 2210 7 -0.06862804611002815 2210 8 -0.021631965584120394 2210 9 0.08106477562595416 2210 10-11 0.031532445030819645 2210 12-13 0.10429921993907243 2210 14-15 0.14746217826214547 2210 16-17 0.16260017150784734 2210 18-19 0.3233262845869618 2210 20-21 0.33237341289476774 2210 22-23 0.21910738494617732 2210 24-25 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-2.307129275710391 2216 120-121 -2.183443046293739 2216 122-123 -2.19989237421996 2216 124-125 -1.969010117821611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30021.0 3 6315.0 4 4485.0 5 3935.0 6 4089.0 7 4583.0 8 4373.0 9 4674.0 10 5342.0 11 6252.0 12 6963.0 13 6975.0 14 6931.0 15 6672.0 16 6473.0 17 5783.0 18 5178.0 19 4982.0 20 4780.0 21 4778.0 22 5610.0 23 7890.0 24 12408.0 25 18938.0 26 22646.0 27 20261.0 28 22009.0 29 27509.0 30 32724.0 31 39310.0 32 52074.0 33 70609.0 34 101583.0 35 159144.0 36 308879.0 37 644861.0 38 325711.0 39 1576.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43093558567383 19.102423569020548 12.194461766689548 24.272179078616073 2 15.441047834696588 21.023332454455783 39.869274856243706 23.666344854603917 3 18.654257906572465 25.927915822714155 29.439073938214698 25.978752332498683 4 11.989340371970581 16.986178068817097 37.97893691790821 33.04554464130411 5 12.855266056435882 39.58979373561517 34.549010343621134 13.005929864327815 6 31.267776283741 37.69954649147411 17.833252542422702 13.19942468236219 7 27.625075100357993 33.46775840750376 21.525182407934185 17.381984084204067 8 25.080928558689518 37.154552872826834 20.181026898470904 17.583491670012744 9 26.05830899723311 16.738105699878343 19.73376256534878 37.469822737539765 10-11 24.016802084044297 28.021906577702538 28.268113952863892 19.69317738538927 12-13 24.344002807795768 25.599764057474122 28.131288863448013 21.924944271282094 14-15 21.71257669697387 27.05784292649073 25.49648920855424 25.733091167981165 16-17 21.99360222711446 29.969944117880036 26.212834305055495 21.823619349950008 18-19 21.90231549453796 29.045713542681234 27.58202027982027 21.469950682960537 20-21 22.79757225007299 28.262650177417857 27.294624253783766 21.64515331872538 22-23 22.43152169472034 28.349076675173617 27.08359699569404 22.135804634411997 24-25 22.179811996184824 28.292090682434868 27.511219972235594 22.01687734914471 26-27 22.180825475217635 28.550028487771435 27.2229741731633 22.046171863847636 28-29 21.959921232480017 28.57993022522977 27.34436128434402 22.11578725794619 30-31 22.228698985215793 28.309333189848125 27.44461059832586 22.01735722661023 32-33 21.93795616892458 28.379451838799323 27.41294227570768 22.269649716568413 34-35 22.318642716559957 28.42009018660156 27.233837414982936 22.02742968185555 36-37 22.338763802254995 28.287939914569392 27.184955324066806 22.188340959108803 38-39 22.067853248899226 28.285849114896962 27.424266433854967 22.222031202348845 40-41 22.33173874530068 28.21785606459925 27.415721156672507 22.03468403342756 42-43 22.142726013908778 28.224347859443572 27.351057530949014 22.281868595698636 44-45 22.069093668991744 28.374744715400173 27.272278913386195 22.283882702221895 46-47 22.17995116285268 28.35177492367172 27.08182476466329 22.38644914881231 48-49 22.100419677410354 28.38374875950053 27.181809395513078 22.334022167576034 50-51 22.063002776127544 28.317322961152897 27.189634794131894 22.430039468587662 52-53 22.196128406152184 28.26925098403965 27.241445792255913 22.29317481755225 54-55 22.024871475125288 28.080025946605197 27.418307801070046 22.476794777199473 56-57 22.102858737064192 28.408097077245557 27.216078574479987 22.272965611210267 58-59 22.13542788944542 28.22723419498533 27.221988753825972 22.41534916174328 60-61 22.200185648651704 28.228709572465004 27.38322595174829 22.187878827134995 62-63 22.086018103436462 28.215975715935233 27.392763856901393 22.30524232372691 64-65 21.93037999908333 28.211155660330363 27.340121000284967 22.518343340301346 66-67 21.992561925828504 28.627642932584045 26.95913293876168 22.42066220282577 68-69 21.992552050079478 28.21631588793847 27.361463223972343 22.429668838009707 70-71 21.95862734821658 28.08478987359934 27.406704037527625 22.549878740656453 72-73 22.052565725839496 28.06800917769186 27.396321898748965 22.483103197719675 74-75 21.90617294529292 28.22595731229944 27.33092396027598 22.536945782131664 76-77 21.92649595131414 28.11462857404756 27.344347034201853 22.614528440436445 78-79 22.083663593976443 28.095113072442786 27.360651596983537 22.460571736597227 80-81 21.98239399781793 28.265679255904786 27.31710233301947 22.434824413257807 82-83 22.060799416526468 28.193648248956677 27.337742597104853 22.407809737412002 84-85 22.086788378592082 28.056678104458744 27.271386570301566 22.58514694664761 86-87 22.12699282582702 27.864114188919885 27.35666600239139 22.6522269828617 88-89 22.103014501461058 27.930376447138304 27.342591132629444 22.624017918771195 90-91 22.206947469158298 27.964115096523624 27.360320907644148 22.46861652667393 92-93 22.18960196505359 27.846703501042587 27.36968828612543 22.594006247778392 94-95 21.965017142208094 27.81429125114705 27.435379205968534 22.78531240067632 96-97 22.082882891859175 27.74838214707391 27.347518295811767 22.821216665255147 98-99 21.988463460732564 27.681041995853185 27.30378241225887 23.02671213115538 100-101 21.826062737352732 27.837943951123894 27.44328197728589 22.892711334237486 102-103 21.935058788361896 27.80487744121164 27.439766839378237 22.820296931048226 104-105 21.85366334892265 27.809409544868764 27.430467756814586 22.906459349393995 106-107 21.808853777986528 27.92205446121224 27.419402663384258 22.849689097416974 108-109 21.919317093462496 27.86351334689216 27.180058810269003 23.03711074937634 110-111 21.791531731657578 28.18573865755058 27.185561055221868 22.837168555569974 112-113 21.872623159719932 28.02629300734057 27.298888015555573 22.80219581738393 114-115 21.794005446875524 28.139690180922976 27.153322319980266 22.912982052221235 116-117 21.78620194832204 28.11205555725853 27.286252913025304 22.815489581394118 118-119 21.766830420396786 28.073082226504102 27.107633496953742 23.052453856145362 120-121 21.81311841064412 28.158277213559725 27.174805405453935 22.853798970342226 122-123 22.03116708397175 28.036971984503655 27.14752668883989 22.784334242684704 124-125 22.06881593177579 27.945552372594374 27.03122844754344 22.95440324808639 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2505.0 1 2004.5 2 1358.5 3 1049.0 4 770.0 5 571.5 6 468.0 7 463.0 8 496.0 9 495.0 10 465.5 11 447.5 12 455.0 13 467.0 14 496.0 15 601.5 16 750.5 17 870.0 18 1129.0 19 1477.5 20 1911.5 21 2643.5 22 3563.0 23 4771.5 24 6294.5 25 8428.5 26 11412.0 27 14957.0 28 18221.5 29 21326.5 30 25536.5 31 29963.0 32 34584.0 33 38647.5 34 43494.0 35 48542.0 36 53255.0 37 58128.0 38 60836.5 39 62599.5 40 64764.5 41 68064.5 42 71387.0 43 74274.5 44 76672.0 45 78364.0 46 77987.5 47 76930.0 48 77378.0 49 76996.0 50 74711.0 51 70637.5 52 67838.5 53 65257.5 54 60711.5 55 55684.5 56 49948.0 57 44527.5 58 40253.5 59 35638.0 60 29940.5 61 23861.0 62 19022.5 63 14950.5 64 11336.5 65 8843.5 66 6606.5 67 5291.5 68 3934.5 69 2412.0 70 1745.5 71 1381.0 72 1156.0 73 1026.0 74 793.0 75 613.0 76 423.0 77 252.0 78 160.0 79 96.5 80 64.5 81 51.0 82 30.0 83 17.5 84 17.0 85 12.0 86 9.0 87 8.0 88 9.0 89 6.5 90 2.5 91 4.0 92 4.5 93 4.5 94 4.0 95 2.5 96 1.0 97 0.5 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06152463526103881 2 0.04877135054296113 3 0.0445368614764119 4 0.04368996366310206 5 0.0434408760709521 6 0.004334124103409213 7 5.978102211598913E-4 8 0.0 9 9.963503685998189E-4 10-11 0.0037363138822493207 12-13 0.002864507309724479 14-15 0.007846259152723573 16-17 0.003238138697949411 18-19 0.005430109508869013 20-21 0.009963503685998187 22-23 0.01693795626619692 24-25 0.003960492715184279 26-27 0.01616578473053206 28-29 0.00764698907900361 30-31 0.0074975365237136375 32-33 0.0025656021991445335 34-35 0.018731386929676595 36-37 0.015792153342307127 38-39 0.027598905210214984 40-41 0.005978102211598913 42-43 0.0016190693489747056 44-45 3.7363138822493205E-4 46-47 0.002117244533274615 48-49 0.003487226290099366 50-51 0.0016688868674046967 52-53 0.0019428832187696468 54-55 0.00632682484060885 56-57 0.00291432482815447 58-59 0.0076968065974336005 60-61 0.015393613194867201 62-63 0.012379653329852748 64-65 0.002889416068939475 66-67 0.004284306584979221 68-69 0.00978914237149322 70-71 0.002889416068939475 72-73 0.0033626824940243888 74-75 0.0013201642383947599 76-77 3.985401474399275E-4 78-79 0.0036366788453893384 80-81 0.0027897810320794926 82-83 0.001843248181909665 84-85 0.011806751867907854 86-87 0.0062770073221788585 88-89 0.0022666970885645878 90-91 9.714416093848234E-4 92-93 0.0026154197175745244 94-95 0.0 96-97 0.001793430663479674 98-99 0.024161496438545607 100-101 0.014546715381557353 102-103 0.0062770073221788585 104-105 0.014422171585482379 106-107 0.033303011070448946 108-109 0.0011707116831047872 110-111 0.0016190693489747056 112-113 0.016365054804252023 114-115 0.03342755486652393 116-117 0.05158604033425562 118-119 0.35709197210617505 120-121 0.11896423401081836 122-123 0.01997682489042637 124-125 0.3185332128413621 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2007326.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.64569615514002 #Duplication Level Percentage of deduplicated Percentage of total 1 85.32346424938248 63.69029387263356 2 9.363088632214073 13.978285382277944 3 2.674022441374641 5.9881280001263155 4 1.054493822546999 3.148537019012617 5 0.5138832782368743 1.91795875232385 6 0.27878172969873927 1.2485913773217878 7 0.17952651655621368 0.9380617264652088 8 0.10751355839292952 0.6420339529885216 9 0.07664966498045096 0.5149410842271617 >10 0.3931353465870542 5.259474057854589 >50 0.02577052109352079 1.3157558943749783 >100 0.009539560352538303 1.0363299216570283 >500 0.0 0.0 >1k 1.306785834593749E-4 0.3216089587364092 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3297 0.16424835826368014 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.981751842999094E-5 0.0 5 0.0 0.0 0.0 4.981751842999094E-5 0.0 6 0.0 0.0 0.0 4.981751842999094E-5 0.0 7 0.0 0.0 0.0 1.9927007371996376E-4 0.0 8 0.0 0.0 0.0 1.9927007371996376E-4 0.0 9 0.0 0.0 0.0 1.9927007371996376E-4 0.0 10-11 0.0 0.0 0.0 2.490875921499547E-4 0.0 12-13 0.0 0.0 0.0 4.981751842999094E-4 0.0 14-15 4.981751842999094E-5 0.0 0.0 5.230839435149049E-4 0.0 16-17 4.981751842999094E-5 0.0 0.0 6.974452580198731E-4 0.0 18-19 1.2454379607497736E-4 0.0 0.0 8.46897813309846E-4 0.0 20-21 1.4945255528997284E-4 0.0 0.0 8.96715331739837E-4 0.0 22-23 1.4945255528997284E-4 0.0 0.0 0.0010461678870298098 0.0 24-25 1.4945255528997284E-4 0.0 0.0 0.0016190693489747056 0.0 26-27 1.4945255528997284E-4 0.0 0.0 0.0022916058477795835 0.0 28-29 1.7436131450496829E-4 0.0 0.0 0.00445866789948419 0.0 30-31 2.9890511057994567E-4 0.0 0.0 0.012678558440432695 0.0 32-33 2.9890511057994567E-4 0.0 0.0 0.029467062151339644 0.0 34-35 2.9890511057994567E-4 0.0 0.0 0.053653467349100245 0.0 36-37 2.9890511057994567E-4 0.0 0.0 0.09236167916920321 0.0 38-39 2.9890511057994567E-4 0.0 0.0 0.14773385090413815 0.0 40-41 2.9890511057994567E-4 0.0 0.0 0.20452582191432783 0.0 42-43 3.487226290099366E-4 0.0 0.0 0.2617412418311724 0.0 44-45 3.487226290099366E-4 0.0 0.0 0.33671660706830875 0.0 46-47 4.7326642508491396E-4 0.0 0.0 0.4263881402422925 0.0 48-49 4.981751842999094E-4 0.0 0.0 0.5195718084655905 0.0 50-51 4.981751842999094E-4 0.0 0.0 0.6178368635687477 0.0 52-53 4.981751842999094E-4 0.0 0.0 0.7160271923942598 0.0 54-55 4.981751842999094E-4 0.0 0.0 0.8276682511958695 0.0 56-57 4.981751842999094E-4 0.0 0.0 0.9637448027873898 0.0 58-59 4.981751842999094E-4 0.0 0.0 1.1146669748710474 0.0 60-61 4.981751842999094E-4 0.0 0.0 1.2743072126799535 0.0 62-63 4.981751842999094E-4 0.0 0.0 1.4360397862629188 0.0 64-65 4.981751842999094E-4 0.0 0.0 1.606241337978983 0.0 66-67 6.476277395898823E-4 0.0 0.0 1.810368619745871 0.0 68-69 6.476277395898823E-4 0.0 0.0 2.042842069499424 0.0 70-71 6.725364988048778E-4 0.0 0.0 2.3071489135297405 0.0 72-73 7.223540172348686E-4 0.0 0.0 2.5718293889482826 0.0 74-75 7.472627764498641E-4 0.0 0.0 2.8365845906444696 0.0 76-77 7.472627764498641E-4 0.0 0.0 3.1281665260152063 0.0 78-79 7.472627764498641E-4 0.0 0.0 3.4679967279853896 0.0 80-81 7.97080294879855E-4 0.0 0.0 3.8508941746383 0.0 82-83 7.97080294879855E-4 0.0 0.0 4.247765435210823 0.0 84-85 8.219890540948505E-4 0.0 0.0 4.6386336848125325 0.0 86-87 8.46897813309846E-4 0.0 0.0 5.037647098677544 0.0 88-89 8.46897813309846E-4 0.0 0.0 5.483339527311458 0.0 90-91 8.46897813309846E-4 0.0 0.0 5.977678762692259 0.0 92-93 8.46897813309846E-4 0.0 0.0 6.495706228086519 0.0 94-95 8.46897813309846E-4 0.0 0.0 7.0176443686775345 0.0 96-97 8.46897813309846E-4 0.0 0.0 7.536917272032545 0.0 98-99 9.216240909548324E-4 0.0 0.0 8.096318186482913 0.0 100-101 0.0010212591278148143 0.0 0.0 8.723495834757284 0.0 102-103 0.0010959854054598008 0.0 0.0 9.391324578070527 0.0 104-105 0.0011458029238897917 0.0 0.0 10.047919470977808 0.0 106-107 0.0011707116831047872 0.0 0.0 10.690042374781175 0.0 108-109 0.0011956204423197827 0.0 0.0 11.348505424629582 0.0 110-111 0.0012205292015347782 0.0 0.0 12.071531978363256 0.0 112-113 0.0012454379607497736 0.0 0.0 12.82302924387967 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2165 0.0 51.39632 1 GTATCAA 3580 0.0 41.387066 1 ATCAACG 3590 0.0 38.280647 3 GTACATG 9330 0.0 38.138874 1 TCAACGC 3605 0.0 38.12042 4 TACATGG 9260 0.0 37.265865 2 CAACGCA 3710 0.0 36.723576 5 ACATGGG 9330 0.0 34.56045 3 AACGCAG 3915 0.0 34.481228 6 TATCAAC 4460 0.0 32.149662 2 CATGGGG 3595 0.0 29.2904 4 ACGCAGA 4730 0.0 28.790693 7 CGCAGAG 4755 0.0 27.763193 8 CATGGGA 6345 0.0 27.28427 4 CGTCGCA 45 0.009556198 26.430544 68-69 GAGTACA 6350 0.0 25.675873 1 AGTACAT 6555 0.0 24.143665 2 GCAGAGT 5995 0.0 21.029806 9 ATGGGAT 2825 0.0 20.639112 5 ATGGGCG 205 5.94332E-5 20.315504 5 >>END_MODULE