FastQCFastQC Report
Thu 26 May 2016
SRR1364224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007326
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78190.3895231766040992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74380.37054270208227263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44900.22368065775065935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41150.075.528171
GTATCAA76400.052.0584681
ATCAACG93400.042.1796463
TCAACGC94100.041.9291154
CAACGCA95950.041.1104435
AACGCAG99200.039.820586
TATCAAC99850.039.4589042
GTACATG85850.035.30091
ACGCAGA114950.034.3636637
TACATGG85950.034.3455852
CGCAGAG115350.034.141358
TGGTATC17250.033.8120962
ACATGGG85550.033.041933
GTGGTAT19150.032.3351
ACCGTAT1352.334902E-630.8464098
GCAGAGT129650.029.9627049
CATGGGA59650.028.034174
GAGTACT90650.027.85940612-13
CAGAGTA127800.026.92854510-11
AGAGTAC124300.025.19808810-11
Produced by FastQC (version 0.11.5)