FastQCFastQC Report
Thu 26 May 2016
SRR1364223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462276
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67310.4603098183926974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.056.6554531
GTATCAA37250.048.879881
ATCAACG41000.041.9333723
TCAACGC42300.040.36064
CAACGCA42700.039.9838835
AACGCAG43450.039.4130946
GTACATG87600.038.989061
TACATGG93550.035.6762922
TATCAAC50750.034.8161772
ACATGGG93150.034.2955063
ACGCAGA51650.032.9255837
CGCAGAG51800.032.485878
TAACACG953.2905705E-431.3095865
CATGGGG33250.030.9507034
CATGGGA57550.029.2521084
GCAGAGT58200.027.7896929
ACTTGAC8200.024.6548798
ATGGGTA7250.024.615815
ATGGGAG26000.024.2528885
ATGGGGA21150.023.9077915
Produced by FastQC (version 0.11.5)