FastQCFastQC Report
Thu 26 May 2016
SRR1364223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462276
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110460.7553977498092015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102170.698705305975069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64600.44177706534197375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.16980378533190724No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.16351222341062835No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.10935008165353188No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.10134885616668808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44250.076.136851
GTATCAA83250.052.481181
ATCAACG107600.040.257563
TCAACGC108200.039.9793244
TGGTATC19300.039.771832
CAACGCA110000.039.262975
AACGCAG113700.038.664146
TATCAAC113200.038.1621972
GTACATG88850.037.114541
GTGGTAT22700.035.1374861
TACATGG93350.034.9946142
ACATGGG91750.034.7605823
ACGCAGA131150.033.519737
CGCAGAG132700.033.173038
CATGGGA58500.031.0221794
GCAGAGT142000.030.7071849
CATGGGG30950.028.837594
GAGTACT97200.028.36760912-13
CAGAGTA139550.027.5802910-11
ATGGGAT25350.026.7526055
Produced by FastQC (version 0.11.5)