FastQCFastQC Report
Thu 26 May 2016
SRR1364222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366724
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32990.241380117712135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17650.046.184251
GTATCAA28850.044.547771
ATCAACG31450.038.9687923
TCAACGC32100.037.994364
CAACGCA32850.037.3093835
AACGCAG34200.035.8156666
TATCAAC35950.035.416132
GTACATG68600.034.086821
TACATGG68450.033.2898252
ACATGGG69100.030.9952243
CGCAGAG41000.029.439358
ACGCAGA41550.029.3358637
CTCGCGC1700.026.24093660-61
GCGTAAA1401.05604544E-425.5000611
CATGGGG28450.024.8848514
CATGGGA46050.024.675964
GCAGAGT47450.024.3142599
TCGCGGA2000.022.30520482-83
CGGTTAC2300.021.98153928-29
TAGCCCT11700.021.3566824
Produced by FastQC (version 0.11.5)