FastQCFastQC Report
Thu 26 May 2016
SRR1364222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366724
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67640.49490606735522313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61830.45239565559688716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35230.25776967405269824No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.1299457681287517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.075.7621461
GTATCAA59700.051.861331
ATCAACG72400.042.5809143
TCAACGC73950.041.7688944
CAACGCA75200.041.1356745
TATCAAC76150.040.4073452
AACGCAG78350.039.8614776
TGGTATC11650.039.3372962
TATAGCG801.1989508E-437.180327
GTGGTAT12850.036.6048431
ACGCAGA89250.034.9265867
CGCAGAG90500.034.444188
GTACATG67300.033.8842851
TACATGG67900.032.607152
ACATGGG66350.032.0221943
GCAGAGT99950.031.009029
GTACCGT1203.7013953E-529.7442576
GAGTACT67650.029.3705312-13
CAGAGTA99400.027.23065810-11
CATGGGG29150.026.3375264
Produced by FastQC (version 0.11.5)