FastQCFastQC Report
Thu 26 May 2016
SRR1364221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402766
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49760.3547277307833238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.049.5623471
GTATCAA29950.041.9226951
ATCAACG30550.037.977383
CAACGCA31100.037.4984055
TCAACGC30950.037.4865574
GTACATG66350.035.2464371
AACGCAG32600.035.2066356
TATCAAC37650.031.9218462
TACATGG71100.031.3807472
ACATGGG68800.030.4407833
ACGCAGA40550.028.3032157
CGCAGAG40700.028.1989048
CATGGGG31100.024.2965684
CATGGGA42250.023.9399814
GCAGAGT48650.023.5942279
GAGTACA49800.022.4642581
AGTACAT50100.021.1389732
CAGAGTA47550.020.26262710-11
CGGTTAC2253.6379788E-1219.82626728-29
GTATTGA5400.018.7334921
Produced by FastQC (version 0.11.5)