##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364221_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1402766 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.875692738489526 33.0 31.0 34.0 27.0 34.0 2 30.853803841838197 33.0 31.0 34.0 27.0 34.0 3 31.00123042617229 33.0 31.0 34.0 27.0 34.0 4 34.27217725550805 37.0 35.0 37.0 30.0 37.0 5 34.46670364123453 37.0 35.0 37.0 31.0 37.0 6 34.478541681221245 37.0 35.0 37.0 31.0 37.0 7 34.40841808255974 37.0 35.0 37.0 31.0 37.0 8 34.37084374728216 37.0 35.0 37.0 31.0 37.0 9 35.80367217340597 39.0 37.0 39.0 30.0 39.0 10-11 35.80118922186594 39.0 37.0 39.0 30.0 39.0 12-13 35.75474419824832 39.0 37.0 39.0 30.0 39.0 14-15 37.06274353669821 40.0 38.0 41.0 30.5 41.0 16-17 37.11620434199289 40.0 38.0 41.0 31.0 41.0 18-19 37.02331322544174 40.0 38.0 41.0 30.0 41.0 20-21 37.018064666523145 40.0 38.0 41.0 30.0 41.0 22-23 37.067746152957795 40.0 37.5 41.0 30.0 41.0 24-25 36.93734913734721 40.0 37.0 41.0 29.5 41.0 26-27 36.9676111340024 40.0 37.0 41.0 30.0 41.0 28-29 37.054451348264784 40.0 37.0 41.0 30.0 41.0 30-31 36.95502100849322 40.0 37.0 41.0 30.0 41.0 32-33 36.77739409138802 40.0 37.0 41.0 30.0 41.0 34-35 36.733167541842334 40.0 37.0 41.0 30.0 41.0 36-37 36.750832997092886 40.0 37.0 41.0 30.0 41.0 38-39 36.586569677337486 40.0 36.5 41.0 29.5 41.0 40-41 36.59170061150613 40.0 37.0 41.0 29.0 41.0 42-43 36.52184469826044 40.0 36.0 41.0 29.0 41.0 44-45 36.51682960664858 40.0 36.0 41.0 29.0 41.0 46-47 36.44687602921656 40.0 36.0 41.0 29.0 41.0 48-49 36.37420995376278 40.0 36.0 41.0 29.0 41.0 50-51 36.276787076390505 40.0 36.0 41.0 29.0 41.0 52-53 36.147210226081896 40.0 35.0 41.0 29.0 41.0 54-55 35.98336322665362 39.5 35.0 41.0 29.0 41.0 56-57 35.81467186972025 39.0 35.0 41.0 28.0 41.0 58-59 35.604529550901574 39.0 35.0 41.0 28.0 41.0 60-61 35.37558545046002 39.0 35.0 41.0 28.0 41.0 62-63 35.098272270642425 38.0 34.5 40.0 28.0 41.0 64-65 34.810509022887636 38.0 34.0 40.0 27.0 41.0 66-67 34.519663293806666 37.0 34.0 40.0 27.0 41.0 68-69 34.290484656742464 37.0 34.0 40.0 27.0 41.0 70-71 34.123216915722224 36.5 34.0 39.0 27.0 41.0 72-73 33.80857035314514 36.0 34.0 39.0 26.5 41.0 74-75 33.46539800651 35.5 34.0 38.5 26.0 40.5 76-77 33.12287330887689 35.0 34.0 37.0 26.0 39.5 78-79 32.77475822767304 35.0 33.0 37.0 26.0 39.0 80-81 32.461224110079655 35.0 33.0 36.5 26.0 39.0 82-83 32.17067529438267 35.0 33.0 36.0 25.5 37.5 84-85 31.898307344204238 35.0 33.0 36.0 25.0 37.0 86-87 31.67797337545963 35.0 33.0 35.0 25.0 37.0 88-89 31.491709950198395 35.0 33.0 35.0 24.5 36.0 90-91 31.33239506802988 35.0 33.0 35.0 24.0 36.0 92-93 31.188020311299248 35.0 33.0 35.0 23.5 36.0 94-95 31.04968612013693 35.0 32.0 35.0 23.0 36.0 96-97 30.9505423570289 35.0 32.0 35.0 22.0 35.0 98-99 30.87499055437614 35.0 32.0 35.0 20.0 35.0 100-101 30.76996697952474 35.0 32.0 35.0 20.0 35.0 102-103 29.79509911132719 34.0 30.5 35.0 18.0 35.0 104-105 30.467386221222924 34.5 31.5 35.0 19.0 35.0 106-107 30.565639957056273 35.0 32.0 35.0 18.5 35.0 108-109 30.531147390227595 35.0 32.0 35.0 18.0 35.0 110-111 30.45634054432457 35.0 32.0 35.0 17.5 35.0 112-113 30.59306897943064 35.0 32.5 35.0 17.5 35.0 114-115 30.576916962629547 35.0 33.0 35.0 16.0 35.0 116-117 30.485486888048328 35.0 32.5 35.0 8.5 35.0 118-119 30.248700424732277 35.0 32.0 35.0 2.0 35.0 120-121 30.18782427004932 35.0 32.0 35.0 2.0 35.0 122-123 30.06318658992305 35.0 32.0 35.0 2.0 35.0 124-125 29.212354376995165 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6421922878568438 1101 2 0.7750536677327453 1101 3 0.6849954660255264 1101 4 0.8129709396410831 1101 5 0.6503271826280539 1101 6 0.6707123896201423 1101 7 0.6781914140991674 1101 8 0.6161100379180056 1101 9 0.7388274978991873 1101 10-11 0.7548620259814882 1101 12-13 0.7211997213892118 1101 14-15 0.8315345393429396 1101 16-17 0.7968748961827714 1101 18-19 1.0436004041504106 1101 20-21 0.9471500834919269 1101 22-23 0.9724773590268612 1101 24-25 1.15912626977574 1101 26-27 1.1667585887379701 1101 28-29 0.9836597574713437 1101 30-31 0.9880322937326582 1101 32-33 1.1146095786099721 1101 34-35 1.162727330685641 1101 36-37 1.0818913946737467 1101 38-39 1.1637697885432061 1101 40-41 1.096531059893742 1101 42-43 1.0036436015979149 1101 44-45 0.9092977762792742 1101 46-47 0.8577183546188252 1101 48-49 0.8894993601804231 1101 50-51 0.8897523580321902 1101 52-53 0.8349358600551398 1101 54-55 0.8406542367840686 1101 56-57 0.8783774676820499 1101 58-59 0.8496254396499765 1101 60-61 0.7212159126228244 1101 62-63 0.5596041415663748 1101 64-65 0.531236944207599 1101 66-67 0.6298685400617074 1101 68-69 0.5951213274069076 1101 70-71 0.6146937790646732 1101 72-73 0.6624206035194646 1101 74-75 0.6365358471156881 1101 76-77 0.5877805815554069 1101 78-79 0.533025666825786 1101 80-81 0.6061109787802366 1101 82-83 0.5734116435425278 1101 84-85 0.5224914011346193 1101 86-87 0.494999959550281 1101 88-89 0.6218939622380333 1101 90-91 0.6168814729362531 1101 92-93 0.611572003501685 1101 94-95 0.6245035895416926 1101 96-97 0.6368201870278192 1101 98-99 0.640197103900956 1101 100-101 0.4972459322686511 1101 102-103 0.45506213417019126 1101 104-105 0.57787222921446 1101 106-107 0.6085405545726061 1101 108-109 0.5576190817349413 1101 110-111 0.5365197806094564 1101 112-113 0.6156083591916612 1101 114-115 0.5440295068125209 1101 116-117 0.6285014950972219 1101 118-119 0.4178957944716579 1101 120-121 0.4068439735035838 1101 122-123 0.3512488770578237 1101 124-125 0.2956401277230114 1102 1 0.2410282833544919 1102 2 0.1612349945314584 1102 3 0.11595391991901849 1102 4 0.2140372835627815 1102 5 0.22645460178277688 1102 6 0.21704834899333747 1102 7 0.15715552433404412 1102 8 0.24756516562167263 1102 9 0.28272144448781233 1102 10-11 0.3124498793969437 1102 12-13 0.22899525301903623 1102 14-15 0.3486222759144866 1102 16-17 0.27703410285995744 1102 18-19 0.46227006773462875 1102 20-21 0.5750327522765701 1102 22-23 0.5706394466911817 1102 24-25 0.7155781961057741 1102 26-27 0.7436312926555004 1102 28-29 0.6812663507563883 1102 30-31 0.717786906575725 1102 32-33 0.798596880741421 1102 34-35 0.8687369691870259 1102 36-37 0.7164366576124053 1102 38-39 0.6946781166488734 1102 40-41 0.6133816207694167 1102 42-43 0.6053441413719298 1102 44-45 0.4959158601288465 1102 46-47 0.33568642658470793 1102 48-49 0.3179107912517267 1102 50-51 0.3112661368937566 1102 52-53 0.4037706248301589 1102 54-55 0.42982930882120485 1102 56-57 0.3939550776984291 1102 58-59 0.48902184245203983 1102 60-61 0.44560679212113286 1102 62-63 0.5468165847201902 1102 64-65 0.5101802517422627 1102 66-67 0.5058896785947411 1102 68-69 0.4391971170001838 1102 70-71 0.428798483165572 1102 72-73 0.3721075864104293 1102 74-75 0.455708936045113 1102 76-77 0.4748670126640917 1102 78-79 0.46876251341546293 1102 80-81 0.5280601195415215 1102 82-83 0.45457142272995554 1102 84-85 0.5019971289774965 1102 86-87 0.4234022972256888 1102 88-89 0.5109992518041508 1102 90-91 0.4578608989411741 1102 92-93 0.4592294699769539 1102 94-95 0.3346603148429068 1102 96-97 0.47380013820749056 1102 98-99 0.42175318075584656 1102 100-101 0.3947938793784722 1102 102-103 0.3183860269807326 1102 104-105 0.45944519736365663 1102 106-107 0.46139981720532575 1102 108-109 0.47881297236804343 1102 110-111 0.5259331992728953 1102 112-113 0.5518928026085632 1102 114-115 0.528332686112865 1102 116-117 0.4168767469095478 1102 118-119 0.1837055975012305 1102 120-121 0.2690059047116691 1102 122-123 0.24875409766361045 1102 124-125 -0.05376476557034593 1103 1 0.22389737924416053 1103 2 -0.06313451690528993 1103 3 0.2766205000537383 1103 4 0.31131937319308634 1103 5 0.2618625547145683 1103 6 0.24376904323786874 1103 7 0.22253832165362297 1103 8 0.22211702376610276 1103 9 0.2846337843956377 1103 10-11 0.23029844977937586 1103 12-13 0.14127655804996664 1103 14-15 0.41912555282769404 1103 16-17 0.2927145878392423 1103 18-19 0.5116735542617974 1103 20-21 0.6015794543438417 1103 22-23 0.49877014351229576 1103 24-25 0.7488025895910866 1103 26-27 0.7316137244098826 1103 28-29 0.6134598920505283 1103 30-31 0.6078554524709148 1103 32-33 0.7561377041251873 1103 34-35 0.7076349848286441 1103 36-37 0.5985390982007885 1103 38-39 0.6349428608210417 1103 40-41 0.710894368087736 1103 42-43 0.5733031852843098 1103 44-45 0.5881213011802515 1103 46-47 0.5087401115864552 1103 48-49 0.4674596708244394 1103 50-51 0.49580439531560927 1103 52-53 0.4372435958658514 1103 54-55 0.2651439573884815 1103 56-57 0.38462452016792525 1103 58-59 0.404348555445587 1103 60-61 0.32064275292603384 1103 62-63 0.32404073023334945 1103 64-65 0.32844294045778355 1103 66-67 0.3283244260796607 1103 68-69 0.4029652136395754 1103 70-71 0.3879949565366587 1103 72-73 0.36524839584576796 1103 74-75 0.38860437897567834 1103 76-77 0.3647072013941468 1103 78-79 0.30203042310710515 1103 80-81 0.3803045580880209 1103 82-83 0.3409749935289099 1103 84-85 0.350539178661041 1103 86-87 0.369029196973262 1103 88-89 0.3113879675215152 1103 90-91 0.29475939696611775 1103 92-93 0.3617531677841406 1103 94-95 0.4463606288763877 1103 96-97 0.35482072764504125 1103 98-99 0.42312610859013944 1103 100-101 0.4407521512838066 1103 102-103 0.3300721988525517 1103 104-105 0.2462155441565841 1103 106-107 0.31589913140734893 1103 108-109 0.28210918575582866 1103 110-111 0.31971166521643823 1103 112-113 0.3827561815778111 1103 114-115 0.2841347258775251 1103 116-117 0.3357673330730897 1103 118-119 0.23696827168713597 1103 120-121 0.3188510916303642 1103 122-123 0.4053094012648266 1103 124-125 0.4277556185152669 1104 1 0.15506624496027044 1104 2 0.2535438037100377 1104 3 0.2362889705489799 1104 4 0.2667145180567374 1104 5 0.2513302986114425 1104 6 0.09625475448341803 1104 7 0.28488061332662085 1104 8 0.17397418182405033 1104 9 0.27658875961832763 1104 10-11 0.1190257245794939 1104 12-13 0.17147960277073082 1104 14-15 0.2474980408950671 1104 16-17 0.36129110867953784 1104 18-19 0.4594150950151459 1104 20-21 0.5210589028587265 1104 22-23 0.5323183148557362 1104 24-25 0.7356481950390545 1104 26-27 0.6024838563528547 1104 28-29 0.46861619430328716 1104 30-31 0.4110563614789129 1104 32-33 0.5817341456172969 1104 34-35 0.6033232771704746 1104 36-37 0.4945466073972824 1104 38-39 0.6587710689504362 1104 40-41 0.5158927720034612 1104 42-43 0.37989957357361703 1104 44-45 0.39838108289672647 1104 46-47 0.31658771593996704 1104 48-49 0.274627039261496 1104 50-51 0.14270780181546883 1104 52-53 0.2780161386561133 1104 54-55 0.29479122276409697 1104 56-57 0.2051999465504366 1104 58-59 0.2623151060794555 1104 60-61 0.23212942942255665 1104 62-63 0.18832416439867217 1104 64-65 0.13021022377034086 1104 66-67 0.2308274282381717 1104 68-69 0.2216006375899653 1104 70-71 0.21866846609796653 1104 72-73 0.24390703902213318 1104 74-75 0.21269784111048295 1104 76-77 0.07904326833848074 1104 78-79 0.10589256702830596 1104 80-81 0.0474658842060407 1104 82-83 0.10941356708559624 1104 84-85 0.15519461446806204 1104 86-87 0.11617999653548239 1104 88-89 0.18580558677181358 1104 90-91 0.15910363434185015 1104 92-93 0.2186243105804806 1104 94-95 0.23680996460131354 1104 96-97 0.2230402196342176 1104 98-99 0.17511361353039945 1104 100-101 0.1343115712211791 1104 102-103 0.11362322034648997 1104 104-105 0.25733303115989514 1104 106-107 0.2445085619852847 1104 108-109 0.24971437530983565 1104 110-111 0.26343164363129645 1104 112-113 0.3641960016003125 1104 114-115 0.30838570632928963 1104 116-117 0.20731960103283953 1104 118-119 0.1557294822790567 1104 120-121 0.13963837919914823 1104 122-123 0.1913524043353867 1104 124-125 0.1529013562102115 1105 1 0.00791727755319016 1105 2 0.015463665151486339 1105 3 0.051575171609652415 1105 4 0.06844993944284994 1105 5 -0.08040855281503667 1105 6 -0.11039881709322685 1105 7 -0.07537398236208048 1105 8 -0.04772445143679249 1105 9 -0.1897450172330437 1105 10-11 -0.09905405118633581 1105 12-13 -0.10126573463593047 1105 14-15 -0.15019947383337495 1105 16-17 -0.1029700380093388 1105 18-19 0.10697891826630013 1105 20-21 0.16887244058728612 1105 22-23 0.11735693107944911 1105 24-25 0.29889121339919456 1105 26-27 0.2818729652208205 1105 28-29 0.1787121741828983 1105 30-31 0.16220834619072377 1105 32-33 0.3663590603374516 1105 34-35 0.3296951048542951 1105 36-37 0.2697312547627462 1105 38-39 0.2664157949806736 1105 40-41 0.1866918206384156 1105 42-43 0.028041509397937148 1105 44-45 0.01213262358793088 1105 46-47 -0.032574963147716574 1105 48-49 0.0024310737051322917 1105 50-51 -0.06480033510722194 1105 52-53 -0.17591636964571933 1105 54-55 -0.1104261136399387 1105 56-57 -0.09780077338111681 1105 58-59 -0.2030604503597857 1105 60-61 -0.2068272776256137 1105 62-63 -0.12796749080311542 1105 64-65 -0.11773045631996837 1105 66-67 -0.08744344977677088 1105 68-69 -0.09000321724771965 1105 70-71 -0.12785336460127183 1105 72-73 -0.18902259709204827 1105 74-75 -0.15921736056458258 1105 76-77 -0.14712890555325941 1105 78-79 -0.1777100496024886 1105 80-81 -0.2780178551410444 1105 82-83 -0.3079592104555573 1105 84-85 -0.19708549073241244 1105 86-87 -0.247026059370409 1105 88-89 -0.23946629575495848 1105 90-91 -0.21805663146743015 1105 92-93 -0.1590313793287912 1105 94-95 -0.1810122989991747 1105 96-97 -0.29209495128356266 1105 98-99 -0.24864455166185095 1105 100-101 -0.3133145864174445 1105 102-103 -0.3175640683385268 1105 104-105 -0.3716164624379914 1105 106-107 -0.30571867818915877 1105 108-109 -0.3269795369338979 1105 110-111 -0.43434964323692427 1105 112-113 -0.3363421566564746 1105 114-115 -0.2997717589085731 1105 116-117 -0.42044857818111936 1105 118-119 -0.3834085504608673 1105 120-121 -0.3632249742118603 1105 122-123 -0.3509803246575025 1105 124-125 -0.36652913929869513 1106 1 0.18342597760996782 1106 2 0.21716493537090642 1106 3 0.21778778029078438 1106 4 0.2613157815615921 1106 5 0.21131976940434072 1106 6 0.3312158421962934 1106 7 0.1327592069907908 1106 8 0.10068037116666062 1106 9 0.2292512756410403 1106 10-11 0.12649940156172335 1106 12-13 0.19899676995622428 1106 14-15 0.3570473450518179 1106 16-17 0.25420174775663895 1106 18-19 0.24169398166593936 1106 20-21 0.40905835350879016 1106 22-23 0.3461509462419272 1106 24-25 0.45251094911339607 1106 26-27 0.34943427752113365 1106 28-29 0.3037290399359591 1106 30-31 0.3139120655624126 1106 32-33 0.47775708097712766 1106 34-35 0.4608557803750202 1106 36-37 0.37339251474026014 1106 38-39 0.4013303529643508 1106 40-41 0.27377777475021503 1106 42-43 0.23669503228081368 1106 44-45 0.10576159928567108 1106 46-47 0.06589576391653651 1106 48-49 0.007194563524450359 1106 50-51 0.057239252099300586 1106 52-53 0.07762632909741995 1106 54-55 0.0826093879353067 1106 56-57 0.11700913900603638 1106 58-59 0.08958964370992106 1106 60-61 0.13839865667031148 1106 62-63 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122-123 -1.9603840861391895 2216 124-125 -1.8534676331027242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25535.0 3 5365.0 4 4183.0 5 3491.0 6 3697.0 7 3718.0 8 3478.0 9 3532.0 10 4111.0 11 4683.0 12 5226.0 13 5142.0 14 5133.0 15 5059.0 16 4750.0 17 4369.0 18 4049.0 19 3731.0 20 3522.0 21 3647.0 22 4101.0 23 5727.0 24 9511.0 25 13274.0 26 17020.0 27 15176.0 28 16100.0 29 20084.0 30 23611.0 31 28196.0 32 36355.0 33 48919.0 34 70250.0 35 109803.0 36 215721.0 37 444202.0 38 217340.0 39 955.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11890796074731 19.539892901801924 12.513811701480357 23.827387435970415 2 15.429795738167698 21.346820030055497 39.73537754453584 23.488006687240965 3 18.357784228281258 26.71314357441892 29.157353455100456 25.77171874219937 4 12.064327293526619 17.655915021445757 37.399920978046445 32.87983670698117 5 12.926443623323912 39.7536210488329 34.09494996608777 13.22498536175542 6 30.873293738294905 38.07283234274446 17.703701644185042 13.350172274775593 7 27.48378551396606 33.82728881246801 21.494299087939616 17.194626585626317 8 24.937516307067607 37.41693197582491 20.01452843881303 17.631023278294457 9 26.028211706853178 17.445220142420144 19.856838455292493 36.669729695434185 10-11 23.85901101396252 28.484383371016087 27.89852140787948 19.758084207141913 12-13 24.29169192996639 26.27971311584974 27.68572490788475 21.742870046299117 14-15 21.83007742808022 27.607614551388735 25.154269773105533 25.408038247425512 16-17 21.96070631395169 30.417389370215986 25.94675056087499 21.675153754957336 18-19 21.83772018782422 29.455380341193326 27.435624792141127 21.27127467884133 20-21 22.842500081988135 28.666723226596662 27.086562358748704 21.404214332666495 22-23 22.646560885416797 28.597905883468556 26.85422286644633 21.901310364668316 24-25 22.258643809942047 28.705276718121866 27.17128071467425 21.86479875726184 26-27 22.084805212815144 28.847340563768608 27.043831439096184 22.024022784320056 28-29 22.02609300087333 28.82075320370007 27.170691714046374 21.98246208138022 30-31 22.197911642807007 28.602323478280706 27.236831739778417 21.962933139133867 32-33 21.846350386818788 28.767883061814015 27.229340910970368 22.15642564039683 34-35 22.30483125594687 28.830818164544127 27.06154299731876 21.802807582190244 36-37 22.367833777285977 28.542720697699618 26.836438149886778 22.253007375127627 38-39 22.029599273513217 28.71180872604182 27.186564415624886 22.072027584820077 40-41 22.210760077993278 28.57270164006887 27.22578483408606 21.990753447851798 42-43 22.0933593918476 28.536496950077368 27.07756709099081 22.292576567084218 44-45 22.036810235806218 28.648266776545128 27.13542995889538 22.179493028753274 46-47 22.14696036021136 28.74643909171531 26.814637436089008 22.291963111984327 48-49 22.04766397747797 28.635421794369563 26.984944559320834 22.331969668831633 50-51 21.980674950935278 28.625531905792883 27.01928156271409 22.37451158055775 52-53 22.239953405444634 28.52419445099908 27.011831851827033 22.224020291729254 54-55 22.049271984734975 28.4133234617788 27.20196680149157 22.335437751994657 56-57 22.044631065659814 28.826086414098302 26.897337142592352 22.231945377649534 58-59 22.070221016257005 28.48486836054716 26.93523790919662 22.50967271399922 60-61 22.089789120268538 28.54469670664852 27.089386283441698 22.27612788964125 62-63 21.96376938945821 28.560560987338857 27.186611136401474 22.289058486801462 64-65 21.8477794772519 28.555632746771188 27.04913270381897 22.547455072157945 66-67 21.840254707323265 28.811698514587498 26.785795760771308 22.56225101731793 68-69 21.832405787430147 28.51665376230014 27.1386313956821 22.51230905458761 70-71 21.675361653468535 28.39261322968738 27.297387083988014 22.63463803285607 72-73 21.913286174871278 28.35881066572564 27.13404516590119 22.5938579935019 74-75 21.735848061253986 28.51474906959511 27.115354523384255 22.63404834576665 76-77 21.79336972108231 28.360273645947654 27.31280406540955 22.533552567560484 78-79 21.8332441019989 28.371251303716132 27.238916363268245 22.55658823101672 80-81 21.709224337788473 28.534729569038532 27.225990287469237 22.530055805703757 82-83 21.90721153400597 28.42866705780619 27.15644178995522 22.507679618232615 84-85 21.79401578845115 28.2784385510558 27.14882816468613 22.778717495806916 86-87 21.738031610101917 28.22092209616632 27.33162731890172 22.709418974830047 88-89 21.859950175959632 28.125025619565758 27.346262109811786 22.668762094662824 90-91 21.844132106031573 28.218744296924903 27.384776496486907 22.55234710055662 92-93 21.84403139304685 28.10196680618023 27.32216654511806 22.73183525565486 94-95 21.640530209600175 27.938515761003618 27.436685805045176 22.98426822435103 96-97 21.74268093682298 27.948958503387296 27.33783357013162 22.970526989658104 98-99 21.58557215970166 27.9573453886208 27.27310324419622 23.183979207481315 100-101 21.40078556186068 28.125866716860493 27.45634242454706 23.017005296731767 102-103 21.697412054739633 27.989240569725094 27.344508872858427 22.968838502676846 104-105 21.52124539552088 28.073517663411106 27.385568467129556 23.019668473938463 106-107 21.307374483178616 28.133695399487422 27.532371309870346 23.02655880746362 108-109 21.412251398328706 28.25507967942878 27.08009860659022 23.2525703156523 110-111 21.314259194702927 28.41469926294095 27.206062148499683 23.06497939385644 112-113 21.342607463105317 28.352392214265628 27.407891185952337 22.897109136676722 114-115 21.192631012599332 28.446881329837737 27.288885370950556 23.07160228661238 116-117 21.116109986998012 28.427358242284928 27.273618797041284 23.182912973675784 118-119 21.110064155260453 28.260045231354585 27.24695446743693 23.38293614594803 120-121 21.047245035783043 28.325396414737153 27.38509905238723 23.242259497092572 122-123 21.194618885238654 28.35826801830306 27.2905655803308 23.15654751612748 124-125 21.16879206606558 28.31195060672399 27.239536362316528 23.279720964893897 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3983.0 1 3254.5 2 2285.5 3 1648.0 4 1025.5 5 688.0 6 547.5 7 550.5 8 595.5 9 601.5 10 539.0 11 468.0 12 431.0 13 441.5 14 512.0 15 573.5 16 628.5 17 718.5 18 886.5 19 1132.0 20 1405.5 21 1805.0 22 2344.0 23 3148.0 24 4238.5 25 5649.0 26 7852.0 27 10429.0 28 12468.5 29 14543.0 30 17549.0 31 20884.5 32 24290.0 33 27541.0 34 31522.0 35 35314.0 36 38847.0 37 42384.5 38 43541.0 39 44304.5 40 45215.0 41 46227.0 42 47169.0 43 48922.5 44 50833.5 45 51877.0 46 51790.5 47 51363.0 48 52125.5 49 52036.0 50 50669.0 51 48323.0 52 46024.5 53 44328.5 54 42504.0 55 39557.0 56 35585.5 57 32056.0 58 29376.5 59 26075.5 60 22222.5 61 18034.5 62 14294.0 63 11049.0 64 8282.5 65 6454.0 66 5007.0 67 4048.5 68 3032.0 69 1993.5 70 1468.5 71 1172.0 72 961.0 73 876.5 74 682.0 75 486.0 76 363.0 77 238.5 78 146.5 79 82.5 80 42.5 81 29.0 82 24.5 83 18.5 84 16.5 85 15.5 86 14.5 87 12.0 88 9.5 89 9.5 90 9.5 91 8.0 92 5.5 93 4.0 94 2.5 95 3.0 96 2.5 97 3.0 98 3.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06237676134152097 2 0.04940239498248461 3 0.04533899452937981 4 0.04448354180241038 5 0.04398452771167821 6 0.004633702271084414 7 5.703018179796203E-4 8 0.0 9 0.001069315908711788 10-11 0.003314879317006543 12-13 0.002815865226274375 14-15 0.007806006133596053 16-17 0.0033505231806302688 18-19 0.005097072498192856 20-21 0.008839678178684114 22-23 0.015505080676320927 24-25 0.004063400453104794 26-27 0.01586151931255819 28-29 0.00648718317951818 30-31 0.006023812952409739 32-33 0.0029940845443930065 34-35 0.01728727385750724 36-37 0.015255573630954842 38-39 0.02855073476260474 40-41 0.005774305907043655 42-43 0.0018178370448100395 44-45 2.8515090898981015E-4 46-47 0.0024237827264133862 48-49 0.0036000302259963524 50-51 0.0019247686356812185 52-53 0.0019247686356812185 54-55 0.005881237497914834 56-57 0.003101016135264185 58-59 0.007770362269972326 60-61 0.015184285903707389 62-63 0.0119406943139483 64-65 0.0031723038625116376 66-67 0.004134688180352247 68-69 0.009980281814643353 70-71 0.0029940845443930065 72-73 0.0034574547715014482 74-75 0.0011762474995829668 76-77 3.2079477261353636E-4 78-79 0.003207947726135364 80-81 0.0029940845443930065 82-83 0.0019247686356812185 84-85 0.011299104768721226 86-87 0.0058099497706673815 88-89 0.00210298795379985 90-91 9.980281814643355E-4 92-93 0.0024950704536608383 94-95 0.0 96-97 0.0017821931811863134 98-99 0.02413089567326268 100-101 0.014471408631232864 102-103 0.006380251588647002 104-105 0.013651599767887159 106-107 0.031651750897868924 108-109 0.0014257545449490507 110-111 0.0020673440901761236 112-113 0.016146670221548 114-115 0.03061807885278086 116-117 0.04797664043753555 118-119 0.36092976305385216 120-121 0.11502274791376467 122-123 0.019140754765941004 124-125 0.32214923943123797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1402766.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.32702138953229 #Duplication Level Percentage of deduplicated Percentage of total 1 87.27875203444233 63.12612165246795 2 8.250138285008518 11.934158564128206 3 2.0938336126451342 4.543222454637188 4 0.7598677743017893 2.1983589106056725 5 0.4145643035192773 1.4992100623987659 6 0.2266564991561378 0.9836033677525492 7 0.15049604852102236 0.7619451644294051 8 0.11044042657886582 0.6390261676350959 9 0.08107298359929892 0.5277390677009723 >10 0.5563285348792395 7.9986183726796805 >50 0.05782074032153529 2.846203634010529 >100 0.019650855951540085 2.1629541580246503 >500 1.8895053761828175E-4 0.09880683983362028 >1k 1.8895053761828175E-4 0.680031583695843 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4976 0.3547277307833238 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.128772724745254E-5 7.128772724745254E-5 20-21 0.0 0.0 0.0 2.8515090898981015E-4 7.128772724745254E-5 22-23 0.0 0.0 0.0 7.841649997219779E-4 7.128772724745254E-5 24-25 0.0 0.0 0.0 0.0013544668177015982 7.128772724745254E-5 26-27 0.0 0.0 0.0 0.0021029879537998496 7.128772724745254E-5 28-29 0.0 0.0 0.0 0.003992112725857342 7.128772724745254E-5 30-31 0.0 0.0 0.0 0.01251099613192792 7.128772724745254E-5 32-33 0.0 0.0 0.0 0.026768541581418427 7.128772724745254E-5 34-35 0.0 0.0 0.0 0.05410738498081647 7.128772724745254E-5 36-37 0.0 0.0 0.0 0.0955255545115864 7.128772724745254E-5 38-39 0.0 0.0 0.0 0.14902699381079954 1.4257545449490507E-4 40-41 0.0 0.0 0.0 0.20562944924527682 2.138631817423576E-4 42-43 0.0 0.0 0.0 0.25995069740783566 2.138631817423576E-4 44-45 0.0 0.0 0.0 0.33148793170065427 2.495070453660839E-4 46-47 0.0 0.0 0.0 0.42016986439648524 2.8515090898981015E-4 48-49 0.0 0.0 0.0 0.5159092820898139 2.8515090898981015E-4 50-51 0.0 0.0 0.0 0.6193834181894913 2.8515090898981015E-4 52-53 0.0 0.0 0.0 0.7213605120169722 2.8515090898981015E-4 54-55 0.0 0.0 0.0 0.8317495576596524 3.207947726135364E-4 56-57 7.128772724745254E-5 0.0 0.0 0.9664833621573377 3.564386362372627E-4 58-59 7.128772724745254E-5 0.0 0.0 1.117114329831205 3.564386362372627E-4 60-61 7.128772724745254E-5 0.0 0.0 1.2821810622726812 3.564386362372627E-4 62-63 7.128772724745254E-5 0.0 0.0 1.4437546960790324 3.564386362372627E-4 64-65 7.128772724745254E-5 0.0 0.0 1.6135620623824645 3.564386362372627E-4 66-67 1.4257545449490507E-4 0.0 0.0 1.8086765718587419 3.564386362372627E-4 68-69 1.4257545449490507E-4 0.0 0.0 2.03526461291477 3.564386362372627E-4 70-71 1.4257545449490507E-4 0.0 0.0 2.297282654412782 3.564386362372627E-4 72-73 1.4257545449490507E-4 0.0 0.0 2.5631502331821556 4.277263634847152E-4 74-75 1.4257545449490507E-4 0.0 0.0 2.8221741901357746 4.277263634847152E-4 76-77 1.4257545449490507E-4 0.0 0.0 3.1122439523056586 4.277263634847152E-4 78-79 1.4257545449490507E-4 0.0 0.0 3.4474744896868046 4.277263634847152E-4 80-81 1.4257545449490507E-4 0.0 0.0 3.8088676229677647 4.277263634847152E-4 82-83 1.4257545449490507E-4 0.0 0.0 4.200664971919764 4.277263634847152E-4 84-85 1.4257545449490507E-4 0.0 0.0 4.584513739283672 4.277263634847152E-4 86-87 1.4257545449490507E-4 0.0 0.0 4.990568633685162 4.277263634847152E-4 88-89 1.4257545449490507E-4 0.0 0.0 5.428703005348005 4.277263634847152E-4 90-91 3.564386362372627E-4 0.0 0.0 5.925721039717244 4.277263634847152E-4 92-93 3.564386362372627E-4 0.0 0.0 6.4431630079428786 4.277263634847152E-4 94-95 3.564386362372627E-4 0.0 0.0 6.958715851396455 4.990140907321678E-4 96-97 3.564386362372627E-4 0.0 0.0 7.4801855762115705 4.990140907321678E-4 98-99 4.277263634847152E-4 0.0 0.0 8.03316447647006 4.990140907321678E-4 100-101 4.277263634847152E-4 0.0 0.0 8.655185540567707 4.990140907321678E-4 102-103 4.277263634847152E-4 0.0 0.0 9.311852440107616 5.34657954355894E-4 104-105 5.703018179796203E-4 0.0 0.0 9.962566814422363 5.703018179796203E-4 106-107 5.703018179796203E-4 0.0 0.0 10.5988454239695 5.703018179796203E-4 108-109 5.703018179796203E-4 0.0 0.0 11.256118269191013 5.703018179796203E-4 110-111 6.059456816033466E-4 0.0 0.0 11.969850994392507 6.059456816033466E-4 112-113 6.415895452270728E-4 0.0 0.0 12.728673207078018 6.415895452270728E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1885 0.0 49.562347 1 GTATCAA 2995 0.0 41.922695 1 ATCAACG 3055 0.0 37.97738 3 CAACGCA 3110 0.0 37.498405 5 TCAACGC 3095 0.0 37.486557 4 GTACATG 6635 0.0 35.246437 1 AACGCAG 3260 0.0 35.206635 6 TATCAAC 3765 0.0 31.921846 2 TACATGG 7110 0.0 31.380747 2 ACATGGG 6880 0.0 30.440783 3 ACGCAGA 4055 0.0 28.303215 7 CGCAGAG 4070 0.0 28.198904 8 CATGGGG 3110 0.0 24.296568 4 CATGGGA 4225 0.0 23.939981 4 GCAGAGT 4865 0.0 23.594227 9 GAGTACA 4980 0.0 22.464258 1 AGTACAT 5010 0.0 21.138973 2 CAGAGTA 4755 0.0 20.262627 10-11 CGGTTAC 225 3.6379788E-12 19.826267 28-29 GTATTGA 540 0.0 18.733492 1 >>END_MODULE