FastQCFastQC Report
Thu 26 May 2016
SRR1364221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402766
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78600.5603215361649769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71390.5089230848195636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43660.31124221716237777No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17400.12404064541056742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.10821476996163294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34300.072.033631
GTATCAA61700.052.0097661
ATCAACG79150.040.5982483
TCAACGC79900.040.291644
CAACGCA80950.039.8340075
AACGCAG84800.038.4450846
TATCAAC84500.038.0291822
TGGTATC15000.036.4986272
GTGGTAT16550.034.5346071
ACGCAGA96050.033.5073857
CGCAGAG97150.033.0667538
GTACATG68300.031.9037481
TACATGG70150.030.5389982
GCAGAGT106800.029.7447669
ACATGGG70000.029.3282073
GAGTACT74150.027.15739612-13
CAGAGTA106650.026.10511610-11
GTACTTT78950.025.4309314-15
AGAGTAC103550.025.27802710-11
GAGTACA51650.024.8980161
Produced by FastQC (version 0.11.5)