FastQCFastQC Report
Thu 26 May 2016
SRR1364220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688519
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35600.21083564946559677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.053.084871
GTATCAA27000.045.8353461
ATCAACG30000.039.8552633
TCAACGC30650.038.8171164
CAACGCA30800.038.4383475
AACGCAG33750.035.0619286
TATCAAC34950.034.5519522
GTACATG68050.033.5740741
TACATGG68300.032.3129772
ACATGGG71500.030.3667073
ACGCAGA39050.029.5418157
CGCAGAG41050.027.8127928
CATGGGG40900.026.0347544
GAGTACA52600.024.6587871
AGTACAT49350.024.3493842
TAGACCG1350.002558961522.0323074
CATGGGA38150.021.5183164
GCAGAGT50650.021.2517269
ATGGGGA24200.020.158625
CAGAGTA46500.019.88714810-11
Produced by FastQC (version 0.11.5)