FastQCFastQC Report
Thu 26 May 2016
SRR1364220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688519
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69410.41107029296087283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64550.3822876733989964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37120.21983762101581328No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.11459746677413758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.073.3065951
GTATCAA62150.050.400631
ATCAACG76100.040.5127643
TCAACGC78550.039.32264
CAACGCA79050.039.0588385
TATCAAC80450.038.544152
AACGCAG84150.037.114726
TGGTATC15350.034.120952
GTGGTAT15750.034.029321
ACGCAGA94700.032.727737
CGCAGAG96650.032.0674178
GTACATG64650.031.2264421
TACATGG65150.030.6039282
ACATGGG65650.029.5549143
GCAGAGT108250.028.301379
GAGTACT74950.027.14535112-13
CAGAGTA103800.027.07899910-11
GTACTTT79800.025.45826514-15
AGAGTAC100400.024.17433710-11
AGTACTT77950.022.66633612-13
Produced by FastQC (version 0.11.5)