FastQCFastQC Report
Thu 26 May 2016
SRR1364219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925433
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19470.10112011168396927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.047.9102821
GTATCAA30950.044.982761
ATCAACG33700.040.421973
TCAACGC34100.039.9457364
CAACGCA34750.039.1995665
AACGCAG35700.038.311126
TATCAAC39000.036.1498832
TACATGG75300.033.6540872
GTACATG76750.033.5660631
ACATGGG78150.031.4364133
ACGCAGA45650.029.828917
CGCAGAG47150.028.3754988
CATGGGG42650.025.8013274
GCAGAGT58950.021.7905929
GTGGTAT13500.021.5951
TGGTATC12850.021.2950122
GTATAGG5950.020.998741
CATGGGA43300.020.3312074
GAGTACA57250.019.433771
ATGGGGA24900.019.3502375
Produced by FastQC (version 0.11.5)