FastQCFastQC Report
Thu 26 May 2016
SRR1364219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925433
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58350.3030487168340836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52180.2710039767678231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27580.14324050745988046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.070.161021
GTATCAA65800.052.1096421
ATCAACG80350.042.137363
TGGTATC16200.041.8726842
TCAACGC81900.041.339894
CAACGCA83350.040.612285
TATCAAC85000.040.182222
AACGCAG86450.039.5688636
GTGGTAT18400.038.1755831
ACGCAGA101600.033.3749437
CGCAGAG102550.032.833738
GTACATG72350.030.8541951
TACATGG71950.030.4339242
ACATGGG73150.029.4466043
GCAGAGT116000.028.8215799
GAGTACT77050.026.63841812-13
CAGAGTA111950.025.50683610-11
CATGGGG38200.024.9228424
GTACTTT83250.024.54734414-15
AGAGTAC106600.024.44310410-11
Produced by FastQC (version 0.11.5)