FastQCFastQC Report
Thu 26 May 2016
SRR1364218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602238
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68620.42827594901631344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.048.4796641
GTATCAA28850.042.0812071
GTACATG82100.038.8530771
TACATGG85400.035.6714672
ATCAACG33850.034.447053
ACATGGG86050.034.2222823
CAACGCA34800.033.677645
TCAACGC34850.033.629324
AACGCAG36050.032.8245746
TATCAAC38400.031.453842
CATGGGG42200.030.1686534
CATGGGA49600.027.1069974
CGCAGAG44850.025.9855168
ACGCAGA44950.025.7954227
GTGGTAT13350.025.4095761
TAAACCG1651.1114227E-525.2388025
AAACCGT1751.7596241E-523.7854426
GTATAGG5500.022.7227251
ATGGGAG22900.022.601565
GAGTACA59750.022.2111361
Produced by FastQC (version 0.11.5)