FastQCFastQC Report
Thu 26 May 2016
SRR1364218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602238
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85000.5305079520021371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78460.48969004604809024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48130.3003923262336806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.11945790825083413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.10778673330678713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38300.075.085711
GTATCAA69450.053.4102631
ATCAACG89400.041.3938983
GTGGTAT16600.041.2475931
TCAACGC89850.041.120374
TATCAAC91350.040.4464232
CAACGCA91800.040.2443855
AACGCAG93850.039.618876
TGGTATC16750.038.3624152
ACGCAGA109500.033.8467337
CGCAGAG110500.033.4327558
GTACATG75650.031.0883061
GCAGAGT121500.030.3079959
ACATGGG78200.028.6825713
TACATGG80250.028.6182
GAGTACT81500.027.59332812-13
CAGAGTA118650.027.42599510-11
GTACTTT86350.025.94015514-15
CATGGGG40250.024.6850534
AGAGTAC118550.024.48843810-11
Produced by FastQC (version 0.11.5)