FastQCFastQC Report
Thu 26 May 2016
SRR1364217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630453
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35400.21711757407297236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.052.006031
GTATCAA29850.044.6527941
ATCAACG32800.039.9049873
CAACGCA33350.039.068495
TCAACGC34000.038.3215944
AACGCAG35100.037.1012766
TATCAAC38700.033.822322
GTACATG67600.031.9525011
ACGCAGA41950.030.9003267
TACATGG69600.030.7740672
CGCAGAG42150.030.6126338
ACATGGG70100.029.3654353
CATGGGG39400.025.972314
GCAGAGT50200.024.2829829
CGATCGT2400.022.30298624-25
TATTCCG1653.2180082E-421.634445
CAGAGTA48550.021.43387410-11
GAGTACA53600.021.203791
CATGGGA37800.020.4611424
AGTACAT52650.020.227692
Produced by FastQC (version 0.11.5)