##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364217_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1630453 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94481717657608 31.0 31.0 34.0 27.0 34.0 2 30.97079216634886 33.0 31.0 34.0 27.0 34.0 3 31.086770977145616 34.0 31.0 34.0 27.0 34.0 4 34.42225933528903 37.0 35.0 37.0 31.0 37.0 5 34.54920074359703 37.0 35.0 37.0 31.0 37.0 6 34.59242983391732 37.0 35.0 37.0 31.0 37.0 7 34.49747769484922 37.0 35.0 37.0 31.0 37.0 8 34.46840969963562 37.0 35.0 37.0 31.0 37.0 9 35.93730576717023 39.0 37.0 39.0 30.0 39.0 10-11 35.9091930279499 39.0 37.0 39.0 30.0 39.0 12-13 35.87531287317083 39.0 37.0 39.0 30.0 39.0 14-15 37.184954120112636 40.0 38.0 41.0 31.0 41.0 16-17 37.223879498519736 40.0 38.0 41.0 31.0 41.0 18-19 37.13042602270657 40.0 38.0 41.0 30.5 41.0 20-21 37.11206793449428 40.0 38.0 41.0 30.0 41.0 22-23 37.14845168796647 40.0 37.5 41.0 30.0 41.0 24-25 37.008733462418114 40.0 37.0 41.0 30.0 41.0 26-27 37.02162895833244 40.0 37.0 41.0 30.0 41.0 28-29 37.10301462231662 40.0 37.0 41.0 30.0 41.0 30-31 37.00728693191401 40.0 37.0 41.0 30.0 41.0 32-33 36.82432366955686 40.0 37.0 41.0 30.0 41.0 34-35 36.77784670885944 40.0 37.0 41.0 30.0 41.0 36-37 36.80002122109622 40.0 37.0 41.0 30.0 41.0 38-39 36.63935758957787 40.0 36.5 41.0 29.5 41.0 40-41 36.64539149549236 40.0 37.0 41.0 30.0 41.0 42-43 36.57691298062563 40.0 36.0 41.0 29.0 41.0 44-45 36.57978917515562 40.0 36.0 41.0 29.5 41.0 46-47 36.512809936870305 40.0 36.0 41.0 29.0 41.0 48-49 36.43885564318628 40.0 36.0 41.0 29.0 41.0 50-51 36.33288785386638 40.0 36.0 41.0 29.0 41.0 52-53 36.20290618619488 40.0 35.0 41.0 29.0 41.0 54-55 36.02981962681537 39.5 35.0 41.0 29.0 41.0 56-57 35.84888279514957 39.0 35.0 41.0 28.5 41.0 58-59 35.63534827437528 39.0 35.0 41.0 28.0 41.0 60-61 35.400282314178945 38.5 35.0 41.0 28.0 41.0 62-63 35.114507133906955 38.0 34.0 40.0 28.0 41.0 64-65 34.81480760254972 37.5 34.0 40.0 28.0 41.0 66-67 34.517531630779914 37.0 34.0 40.0 27.0 41.0 68-69 34.29160178183609 37.0 34.0 39.5 27.5 41.0 70-71 34.123827243103605 36.0 34.0 39.0 27.0 41.0 72-73 33.81315254104227 36.0 34.0 39.0 27.0 41.0 74-75 33.471307973918904 35.0 34.0 38.5 26.5 40.0 76-77 33.13099150972153 35.0 33.5 37.0 26.0 39.5 78-79 32.78509929449055 35.0 33.0 37.0 26.0 39.0 80-81 32.4749833328529 35.0 33.0 36.0 26.0 39.0 82-83 32.194297842378774 35.0 33.0 36.0 26.0 37.0 84-85 31.936434230241534 35.0 33.0 36.0 25.0 37.0 86-87 31.715881721214902 35.0 33.0 35.0 25.0 37.0 88-89 31.539975700004845 35.0 33.0 35.0 25.0 36.0 90-91 31.386698052627093 35.0 33.0 35.0 24.0 36.0 92-93 31.249941887315977 35.0 33.0 35.0 24.0 36.0 94-95 31.112488983123097 35.0 32.0 35.0 23.5 35.5 96-97 31.016773865913336 35.0 32.0 35.0 23.0 35.0 98-99 30.937460018780058 35.0 32.0 35.0 22.5 35.0 100-101 30.83529730694476 35.0 32.0 35.0 20.0 35.0 102-103 29.8684840961377 34.0 30.5 35.0 19.0 35.0 104-105 30.539631317186082 34.5 31.5 35.0 19.0 35.0 106-107 30.637927005562258 35.0 32.0 35.0 19.0 35.0 108-109 30.59764985559228 35.0 32.0 35.0 19.0 35.0 110-111 30.52577197870776 35.0 32.0 35.0 18.0 35.0 112-113 30.663655131426665 35.0 32.5 35.0 19.0 35.0 114-115 30.650966939862727 35.0 33.0 35.0 18.0 35.0 116-117 30.55660206089964 35.0 32.5 35.0 13.5 35.0 118-119 30.316702474711015 35.0 32.0 35.0 2.0 35.0 120-121 30.254562075693073 35.0 32.0 35.0 2.0 35.0 122-123 30.119386452722036 35.0 32.0 35.0 2.0 35.0 124-125 29.25619873740611 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.491770705230298 1101 2 0.5280093293073094 1101 3 0.5962331425608447 1101 4 0.5089314176156705 1101 5 0.4054247129796593 1101 6 0.45106148128567014 1101 7 0.4358679511058199 1101 8 0.48021186669085125 1101 9 0.5211022920526531 1101 10-11 0.5170285110185731 1101 12-13 0.4634816558018642 1101 14-15 0.6058471350792516 1101 16-17 0.6109560545435926 1101 18-19 0.7257416613143235 1101 20-21 0.7102117249295148 1101 22-23 0.61650519334151 1101 24-25 0.8371265233736551 1101 26-27 0.8406903480984909 1101 28-29 0.6984419348978719 1101 30-31 0.7899807921438793 1101 32-33 0.940444773677477 1101 34-35 0.841880517151381 1101 36-37 0.8701565426293669 1101 38-39 0.9288104382024329 1101 40-41 0.7907904999676774 1101 42-43 0.7944549334831024 1101 44-45 0.6488372999795757 1101 46-47 0.5994663624261207 1101 48-49 0.5046617683884307 1101 50-51 0.5082685425758839 1101 52-53 0.5310937532517102 1101 54-55 0.47888728435977157 1101 56-57 0.41132168435959215 1101 58-59 0.4779104849668485 1101 60-61 0.3114906915011417 1101 62-63 0.36216967346600626 1101 64-65 0.3211231184855734 1101 66-67 0.2983017297632031 1101 68-69 0.228049167402105 1101 70-71 0.24848445398536256 1101 72-73 0.24776865958707361 1101 74-75 0.31449071008901086 1101 76-77 0.29609785562430346 1101 78-79 0.28384546071594485 1101 80-81 0.28033497018960674 1101 82-83 0.2614304010418351 1101 84-85 0.29866818214842894 1101 86-87 0.3504008007742776 1101 88-89 0.3124881456349051 1101 90-91 0.33817569119789326 1101 92-93 0.2898548616558543 1101 94-95 0.38410269041988343 1101 96-97 0.4055060413620062 1101 98-99 0.3572238549118367 1101 100-101 0.3081244784184882 1101 102-103 0.21294644267938878 1101 104-105 0.2960894451597227 1101 106-107 0.3671271789036332 1101 108-109 0.46449474188833406 1101 110-111 0.3786454386875704 1101 112-113 0.4716233806528649 1101 114-115 0.5028933990427262 1101 116-117 0.46439705406110576 1101 118-119 0.23069395999554487 1101 120-121 0.2580348812895572 1101 122-123 0.32025073830541473 1101 124-125 0.24754014591358953 1102 1 0.09837098335055572 1102 2 0.03479401479562583 1102 3 -0.0220542587902699 1102 4 0.15757519073316928 1102 5 -0.062046722166329005 1102 6 0.07705915604121572 1102 7 0.12417138201325173 1102 8 0.09531679387959002 1102 9 0.08989621886072285 1102 10-11 0.06790743779822606 1102 12-13 0.041709104339673786 1102 14-15 0.036993372643379985 1102 16-17 0.055488161240639045 1102 18-19 0.09975419590180223 1102 20-21 0.19825800468042587 1102 22-23 0.10657656286362283 1102 24-25 0.3375150998467831 1102 26-27 0.28525494713942123 1102 28-29 0.12570604758344928 1102 30-31 0.28668486110861124 1102 32-33 0.4374055854250045 1102 34-35 0.3488674364084403 1102 36-37 0.2593568510047888 1102 38-39 0.27713502286860603 1102 40-41 0.24145043190065252 1102 42-43 0.2419181869503788 1102 44-45 0.13873164837136187 1102 46-47 0.14519667313105344 1102 48-49 0.10299629606693372 1102 50-51 0.023774137650967475 1102 52-53 0.06882127068038812 1102 54-55 0.03334631701830659 1102 56-57 0.008118241094422274 1102 58-59 -0.017485633991356053 1102 60-61 0.09355788282743305 1102 62-63 0.0049223005519110075 1102 64-65 -0.022574325592081834 1102 66-67 -0.0039060983704075625 1102 68-69 0.010033796215253687 1102 70-71 -0.08982680704427537 1102 72-73 -0.10137045309849668 1102 74-75 -0.02780681111801897 1102 76-77 0.07066490845862461 1102 78-79 -0.007942800705741604 1102 80-81 0.031635462936890235 1102 82-83 0.03334533288958852 1102 84-85 -0.052320597162154314 1102 86-87 -0.05324199934844742 1102 88-89 -0.10986269852684316 1102 90-91 -0.028871053033739713 1102 92-93 0.014872066618270452 1102 94-95 -0.010605780458455172 1102 96-97 -0.049400799714565125 1102 98-99 -0.10839125534196725 1102 100-101 -0.15127926754038867 1102 102-103 -0.22402213882206468 1102 104-105 -0.14639946127937264 1102 106-107 -0.08543151327775078 1102 108-109 -0.1454873145254787 1102 110-111 -0.12247545839941054 1102 112-113 -0.017770976261505922 1102 114-115 0.05346595675481325 1102 116-117 0.015834831400130867 1102 118-119 -0.0578932728925281 1102 120-121 -0.14937996036087142 1102 122-123 -0.07124063542116943 1102 124-125 -0.1561723440723739 1103 1 0.229751163673086 1103 2 0.03360469962870738 1103 3 0.16570807248098163 1103 4 0.286998623765065 1103 5 0.20245241582928486 1103 6 0.21610422706471866 1103 7 0.3289467564277757 1103 8 0.30337737564402545 1103 9 0.35524036848524787 1103 10-11 0.4051260630716058 1103 12-13 0.39708364698091003 1103 14-15 0.4825096953890693 1103 16-17 0.3575092674923539 1103 18-19 0.49980875066395214 1103 20-21 0.6980372767533609 1103 22-23 0.5454323470118112 1103 24-25 0.7070696991815595 1103 26-27 0.7203172536243727 1103 28-29 0.6086340191053736 1103 30-31 0.7216251933944378 1103 32-33 0.822050021293002 1103 34-35 0.6864866815349444 1103 36-37 0.6451497660386778 1103 38-39 0.8071942341804075 1103 40-41 0.6646202984550129 1103 42-43 0.5947456841163188 1103 44-45 0.5676379499446043 1103 46-47 0.4485223961996212 1103 48-49 0.45375129945570336 1103 50-51 0.4311661833673597 1103 52-53 0.3767691130537827 1103 54-55 0.26466446932360554 1103 56-57 0.4035746087614882 1103 58-59 0.49366624194691866 1103 60-61 0.38254563220505844 1103 62-63 0.3529411139377885 1103 64-65 0.35363147077131885 1103 66-67 0.38717983854713367 1103 68-69 0.49730799937498205 1103 70-71 0.5526219758026869 1103 72-73 0.49377965345766484 1103 74-75 0.5354339196880389 1103 76-77 0.5643237625488311 1103 78-79 0.463139082860593 1103 80-81 0.4872594521568985 1103 82-83 0.37076582501767064 1103 84-85 0.3560465304086229 1103 86-87 0.5189459971448152 1103 88-89 0.5172725839381762 1103 90-91 0.4109613254133251 1103 92-93 0.3740385744782664 1103 94-95 0.3437950148382356 1103 96-97 0.4665036216232856 1103 98-99 0.44284084421033043 1103 100-101 0.44380651564161866 1103 102-103 0.2693465928295389 1103 104-105 0.2951410897593121 1103 106-107 0.380486795859138 1103 108-109 0.3483976944984768 1103 110-111 0.3302313812837845 1103 112-113 0.30978811094088243 1103 114-115 0.3031241887855032 1103 116-117 0.3675425858453387 1103 118-119 0.1684112149217043 1103 120-121 0.1397101904049336 1103 122-123 0.27018311931656314 1103 124-125 0.06695529859072735 1104 1 0.2286354363216141 1104 2 0.26913082869095817 1104 3 0.2673925924164493 1104 4 0.3103750691947411 1104 5 0.218604363540166 1104 6 0.28420437501277007 1104 7 0.3773324588058742 1104 8 0.3392095141377638 1104 9 0.36278783991105 1104 10-11 0.3324643193071992 1104 12-13 0.41354388777575224 1104 14-15 0.4614438169087407 1104 16-17 0.41930571902264546 1104 18-19 0.5269785059705825 1104 20-21 0.5568308883986788 1104 22-23 0.5728883744678441 1104 24-25 0.7900657859799338 1104 26-27 0.7076764215919056 1104 28-29 0.5787638447485008 1104 30-31 0.6698662100765205 1104 32-33 0.7514142035088938 1104 34-35 0.7310578686916855 1104 36-37 0.6277581212706096 1104 38-39 0.7003869923921826 1104 40-41 0.5608103505385955 1104 42-43 0.4958651631825646 1104 44-45 0.42847787732445397 1104 46-47 0.4822176884500422 1104 48-49 0.28817181448457774 1104 50-51 0.3851163312338599 1104 52-53 0.4910137215949675 1104 54-55 0.4649840175518918 1104 56-57 0.3843095533747629 1104 58-59 0.34778570633253736 1104 60-61 0.439078264783312 1104 62-63 0.384591721634024 1104 64-65 0.2922607573093572 1104 66-67 0.3693265697432935 1104 68-69 0.4057498469803278 1104 70-71 0.4478692056506688 1104 72-73 0.3788205879282529 1104 74-75 0.42584877778350716 1104 76-77 0.4057917922297918 1104 78-79 0.4497992900531145 1104 80-81 0.40314274446604514 1104 82-83 0.3490360561396173 1104 84-85 0.39700220634528804 1104 86-87 0.4233417019207515 1104 88-89 0.40015791359156694 1104 90-91 0.43314293124511494 1104 92-93 0.3828249273710824 1104 94-95 0.4625710423595102 1104 96-97 0.4426631053873322 1104 98-99 0.28761935438808095 1104 100-101 0.3434840312887175 1104 102-103 0.34648837339337035 1104 104-105 0.36047720262394023 1104 106-107 0.4487174382342687 1104 108-109 0.32598935459964196 1104 110-111 0.4057640024437852 1104 112-113 0.47533089435438924 1104 114-115 0.47408249092251253 1104 116-117 0.4092893842461258 1104 118-119 0.2833017708768999 1104 120-121 0.47829347765742014 1104 122-123 0.5187244362989425 1104 124-125 0.3339990941516362 1105 1 0.32952484301921103 1105 2 0.2720209237931499 1105 3 0.3405125002545297 1105 4 0.3166760786206666 1105 5 0.2684018060067004 1105 6 0.095180113266494 1105 7 0.23233096860059277 1105 8 0.2465006821877651 1105 9 0.2442683513374604 1105 10-11 0.19838046440201396 1105 12-13 0.31597683606412375 1105 14-15 0.1879863800819095 1105 16-17 0.2514190408558008 1105 18-19 0.32221250834086135 1105 20-21 0.4918381186539591 1105 22-23 0.37600265264150323 1105 24-25 0.5899663386102674 1105 26-27 0.5843197868668142 1105 28-29 0.44408942700733434 1105 30-31 0.46131975731702823 1105 32-33 0.6576539489396822 1105 34-35 0.6403663331580631 1105 36-37 0.5398518145412012 1105 38-39 0.590536534743606 1105 40-41 0.5278863469942152 1105 42-43 0.5226203691168934 1105 44-45 0.4449481580149097 1105 46-47 0.33632884873291147 1105 48-49 0.3116877054918632 1105 50-51 0.21969877771942947 1105 52-53 0.25289422017651475 1105 54-55 0.3084314293613488 1105 56-57 0.3367850502854708 1105 58-59 0.32802392844237005 1105 60-61 0.29569317064950695 1105 62-63 0.3134552419103471 1105 64-65 0.350012734967315 1105 66-67 0.3217993070145795 1105 68-69 0.37970838415066055 1105 70-71 0.4312265729345981 1105 72-73 0.4573245522757219 1105 74-75 0.42980835859929556 1105 76-77 0.45210958026338943 1105 78-79 0.40677876937439095 1105 80-81 0.23799262509909624 1105 82-83 0.3585352367775769 1105 84-85 0.3588584702751909 1105 86-87 0.23088520136430546 1105 88-89 0.2681904512455944 1105 90-91 0.23556052590326004 1105 92-93 0.32555362872491145 1105 94-95 0.3139812682786456 1105 96-97 0.2922662692386666 1105 98-99 0.2680387091944212 1105 100-101 0.27528857455835976 1105 102-103 0.2720072106920597 1105 104-105 0.3362729070193957 1105 106-107 0.3842023369856058 1105 108-109 0.28620664359008785 1105 110-111 0.32279443976133493 1105 112-113 0.38355707841531483 1105 114-115 0.4261543278488986 1105 116-117 0.4130016010193138 1105 118-119 0.30210369600136033 1105 120-121 0.4132068801724067 1105 122-123 0.31525847971258614 1105 124-125 0.2500518664853999 1106 1 0.21308234083685917 1106 2 0.2192874932410156 1106 3 0.3287268610504306 1106 4 0.20555778020968773 1106 5 0.15557900772239464 1106 6 0.10539411194931603 1106 7 0.20739875382250972 1106 8 0.16242195715557273 1106 9 0.2553824007858765 1106 10-11 0.2397342853450226 1106 12-13 0.25319532247584675 1106 14-15 0.31757569565330357 1106 16-17 0.2850688426290233 1106 18-19 0.4101299649499168 1106 20-21 0.4305930489567018 1106 22-23 0.3106180945764194 1106 24-25 0.49925352440503445 1106 26-27 0.4908656783578209 1106 28-29 0.47021626615016743 1106 30-31 0.44334410894595067 1106 32-33 0.5683971178603571 1106 34-35 0.5797586078465145 1106 36-37 0.6070422609708217 1106 38-39 0.6208382174279166 1106 40-41 0.4745342801751562 1106 42-43 0.42754134951110245 1106 44-45 0.4025726042625166 1106 46-47 0.3119543102923288 1106 48-49 0.260187663160778 1106 50-51 0.2418946130385251 1106 52-53 0.29866672437870534 1106 54-55 0.22429332983351458 1106 56-57 0.13762586924447362 1106 58-59 0.10910841906310509 1106 60-61 0.04983432372282692 1106 62-63 0.06075960809990022 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122-123 -1.905679266167219 2216 124-125 -1.7151760363367146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27785.0 3 5476.0 4 4035.0 5 3556.0 6 3761.0 7 3764.0 8 3804.0 9 4041.0 10 4503.0 11 5158.0 12 5711.0 13 5703.0 14 5785.0 15 5724.0 16 5451.0 17 5048.0 18 4842.0 19 4485.0 20 4372.0 21 4372.0 22 5091.0 23 7027.0 24 11069.0 25 15879.0 26 19674.0 27 17968.0 28 20121.0 29 24286.0 30 28684.0 31 34676.0 32 44293.0 33 58980.0 34 83891.0 35 130854.0 36 258030.0 37 514288.0 38 237134.0 39 1132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.62198437490411 18.8110566271641 12.33431322609747 24.23264577183432 2 15.859211954637473 20.7410039229836 39.25511788894023 24.144666233438695 3 18.963858378842733 25.245873473645457 29.003988464134505 26.786279683377305 4 12.572613013061906 16.95869364993947 36.904192855008375 33.56450048199026 5 13.636634180374092 38.57936949634691 34.00629435980446 13.77770196347455 6 31.945571471943918 36.95711775367319 17.456751278064516 13.640559496318378 7 28.119282626175522 32.88760689553233 21.06011418954434 17.93299628874781 8 25.22660880135766 36.56161815151985 19.95384104908268 18.25793199803981 9 25.82442418952909 17.11316140847783 19.725338114043993 37.33707628794909 10-11 24.42873453555844 27.571902120561692 27.75765482382014 20.241708520059728 12-13 24.7268788502538 25.193294700971286 27.642491591082504 22.437334857692413 14-15 22.180840019861 26.71853958445849 25.025661974673234 26.07495842100727 16-17 22.55688003900871 29.51236042578378 25.77537483018023 22.155384705027277 18-19 22.39677668852465 28.481580595360693 27.19699725154919 21.92464546456547 20-21 23.185206858141097 27.887941821607853 26.9903747540324 21.936476566218648 22-23 22.991792129019476 27.810577023052645 26.65970843517389 22.537922412753993 24-25 22.773448053893638 27.78253633395312 26.98656431119669 22.457451300956546 26-27 22.58732057416268 28.08511225616489 26.769568151147098 22.557999018525337 28-29 22.462438701248516 28.040249396596405 26.920172847911626 22.577139054243453 30-31 22.611322467518328 27.805077767401638 26.950318721372962 22.633281043707072 32-33 22.394295187783708 27.90289944940331 26.987303217037645 22.715502145775336 34-35 22.83504874799829 27.988255209044212 26.677563284063403 22.499132758894092 36-37 22.91514910424584 27.725363195179053 26.60014593135828 22.759341769216825 38-39 22.531999528841293 27.829866308716927 27.03276148081255 22.605372681629227 40-41 22.86337721921165 27.717903659609966 26.848803795469113 22.569915325709275 42-43 22.603556175159767 27.78264526905914 26.82151933399226 22.79227922178883 44-45 22.624071803621952 27.961503808464794 26.75364485935452 22.660779528558734 46-47 22.61322235988273 28.11542424651317 26.484494792752788 22.786858600851314 48-49 22.720735032093252 27.87638654199417 26.691435563556293 22.711442862356286 50-51 22.552527650731985 27.93808002443532 26.681446026102364 22.827946298730335 52-53 22.802335212899425 27.787923366205042 26.666378399066016 22.743363021829524 54-55 22.583082775481763 27.612763300420397 26.807359347187926 22.996794576909913 56-57 22.53450203660304 28.013488623420717 26.717925725476583 22.734083614499657 58-59 22.6634681381043 27.74939230206344 26.665809990965034 22.92132956886722 60-61 22.753171438482482 27.659366298115984 26.892902743780034 22.6945595196215 62-63 22.5231779162893 27.7532027680086 26.852247435163328 22.87137188053877 64-65 22.43121146874704 27.66966784806431 26.76796407782443 23.13115660536422 66-67 22.358893554094216 28.037744308652247 26.562945157650788 23.040416979602753 68-69 22.390985187032157 27.692604004981924 26.88486650724762 23.0315443007383 70-71 22.211805648195597 27.67296291832537 27.000097214542684 23.11513421893635 72-73 22.406266924517343 27.57662072091467 26.846401590774292 23.170710763793693 74-75 22.27986536100204 27.736081313319033 26.775876852420886 23.208176473258042 76-77 22.4372312280946 27.56309076919633 26.90704428873405 23.09263371397502 78-79 22.422475621877123 27.659390464893264 26.876610219598607 23.041523693631007 80-81 22.25198293437487 27.754089150472396 26.942484476316412 23.05144343883632 82-83 22.32352486586344 27.69199253202227 26.862854416141037 23.121628185973254 84-85 22.366494927725807 27.537984934531366 26.778148231182964 23.317371906559867 86-87 22.368468669055105 27.29461043099255 27.16411721878636 23.17280368116598 88-89 22.385209968581762 27.28574955264663 27.094848114522463 23.234192364249143 90-91 22.35127399698057 27.5417456458826 27.100944254601288 23.006036102535543 92-93 22.222866229048694 27.351000587272896 27.049636059475564 23.37649712420285 94-95 22.261849927596806 27.22188250749945 27.087226678720576 23.429040886183163 96-97 22.324472254490544 27.177682397115845 27.07697252129198 23.42087282710163 98-99 22.104181198135308 27.106748552067593 27.03923665236678 23.749833597430314 100-101 21.981458427453575 27.252682676078845 27.191801640076836 23.574057256390745 102-103 22.13682141550457 27.185548637824418 27.132032570890384 23.545597375780623 104-105 22.00916185806113 27.212393044692273 27.174208412641843 23.604236684604754 106-107 21.854197981533176 27.390410748795972 27.166906960336206 23.588484309334643 108-109 21.964530335606653 27.38863328233469 26.867576178266482 23.779260203792177 110-111 21.762382595687562 27.48031416377401 27.16597865523896 23.591324585299464 112-113 21.904737368030723 27.415012430921433 27.248837419005785 23.431412782042056 114-115 21.683725978181545 27.59648222446019 26.988488300718334 23.73130349663993 116-117 21.569641737824213 27.501743463431144 27.19207735853861 23.736537440206032 118-119 21.687657717577544 27.50800764400294 26.95960675673721 23.84472788168231 120-121 21.626011966914604 27.529644808785115 27.10405763080389 23.740285593496395 122-123 21.757804328796134 27.437117865623655 27.104050969448878 23.701026836131334 124-125 21.621764642178533 27.47445187205261 27.02096029456647 23.882823191202387 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2680.0 1 2231.5 2 1579.5 3 1107.5 4 708.0 5 507.0 6 424.5 7 396.5 8 404.5 9 428.0 10 427.5 11 401.5 12 390.0 13 394.0 14 427.5 15 481.0 16 560.0 17 664.0 18 844.0 19 1079.0 20 1331.5 21 1792.0 22 2410.0 23 3177.0 24 4175.0 25 5758.5 26 8134.0 27 10903.5 28 13264.0 29 15765.0 30 19116.0 31 22330.5 32 25542.5 33 28607.5 34 32107.0 35 35654.5 36 38862.0 37 42530.0 38 44853.5 39 46003.5 40 47756.0 41 50743.0 42 53584.5 43 55914.5 44 57981.0 45 59122.0 46 59422.5 47 59611.0 48 61847.5 49 63169.5 50 61501.5 51 59616.5 52 58135.0 53 57348.5 54 55567.0 55 51413.0 56 46700.0 57 42464.5 58 38655.0 59 34981.0 60 30697.0 61 24955.5 62 19328.0 63 14990.0 64 11343.5 65 8437.0 66 6511.0 67 5528.0 68 4377.0 69 2948.0 70 2017.5 71 1622.0 72 1356.0 73 1103.0 74 844.5 75 635.5 76 430.5 77 227.0 78 150.5 79 94.5 80 48.5 81 31.0 82 23.5 83 21.5 84 16.0 85 12.5 86 10.5 87 9.5 88 8.5 89 6.5 90 4.0 91 1.0 92 1.5 93 2.5 94 4.0 95 4.0 96 1.5 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06274329894820642 2 0.05066076728369355 3 0.046183483976539035 4 0.04612215132849582 5 0.04538615955197727 6 0.0035572935865063266 7 3.679958882592752E-4 8 0.0 9 7.359917765185504E-4 10-11 0.0036492925585711457 12-13 0.0025146385697717138 14-15 0.007513249385293535 16-17 0.0029439671060742015 18-19 0.004906611843457003 20-21 0.009629225742784367 22-23 0.015302495686781527 24-25 0.003465294614441508 26-27 0.01551715995493277 28-29 0.007237252469099079 30-31 0.006777257608774985 32-33 0.0024533059217285015 34-35 0.01738780572025075 36-37 0.01438250596613334 38-39 0.026311706010538175 40-41 0.005397273027802703 42-43 0.0013186519329290694 44-45 3.679958882592752E-4 46-47 0.0020239773854260137 48-49 0.0029439671060742015 50-51 0.0015946488491235258 52-53 0.0014413172290154944 54-55 0.00558127097193234 56-57 0.0025759712178149265 58-59 0.007359917765185503 60-61 0.014627836558306188 62-63 0.0112545409159295 64-65 0.002422639597706895 66-67 0.0037719578546575707 68-69 0.009721224714849186 70-71 0.0023613069496636824 72-73 0.0031892976982470515 74-75 0.001165320312821038 76-77 2.759969161944564E-4 78-79 0.002851968134009383 80-81 0.0025146385697717138 82-83 0.0015333162010803133 84-85 0.010334551195281311 86-87 0.006133264804321253 88-89 0.0018706457653179822 90-91 8.279907485833691E-4 92-93 0.002177309005534045 94-95 0.0 96-97 0.0016559814971667383 98-99 0.022693079775988636 100-101 0.013769179485701215 102-103 0.006225263776386073 104-105 0.013247851977333906 106-107 0.030850321965735904 108-109 0.0010733213407562194 110-111 0.0018093131172747697 112-113 0.014965166122543857 114-115 0.030819655641714294 116-117 0.04872878887033236 118-119 0.36256794890745087 120-121 0.11527471199721795 122-123 0.01839979441296376 124-125 0.32414304490837825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1630453.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.27401217076702 #Duplication Level Percentage of deduplicated Percentage of total 1 88.31307177250994 70.00931526531775 2 7.950343395890617 12.6051123825522 3 1.92266126021528 4.572512164277051 4 0.6772547698708583 2.147548114778098 5 0.33930315967226504 1.3448961404719422 6 0.1922151383904721 0.9142599132103172 7 0.1199611174818965 0.6656859361095073 8 0.0831893771090913 0.5275804554739705 9 0.060058929316738924 0.4285001064256558 >10 0.3122063765160012 4.414747976106259 >50 0.022606980128714677 1.2035075584309394 >100 0.006993237668674215 0.788928083222094 >500 0.0 0.0 >1k 1.344852294751246E-4 0.3774059036242596 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3540 0.21711757407297236 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.133264804321253E-5 2 0.0 0.0 0.0 0.0 6.133264804321253E-5 3 0.0 0.0 0.0 0.0 6.133264804321253E-5 4 0.0 0.0 0.0 0.0 6.133264804321253E-5 5 0.0 0.0 0.0 0.0 6.133264804321253E-5 6 0.0 0.0 0.0 0.0 6.133264804321253E-5 7 0.0 0.0 0.0 1.2266529608642506E-4 6.133264804321253E-5 8 0.0 0.0 0.0 1.2266529608642506E-4 6.133264804321253E-5 9 0.0 0.0 0.0 2.4533059217285013E-4 6.133264804321253E-5 10-11 3.0666324021606266E-5 0.0 0.0 2.4533059217285013E-4 6.133264804321253E-5 12-13 6.133264804321253E-5 0.0 0.0 2.4533059217285013E-4 6.133264804321253E-5 14-15 6.133264804321253E-5 0.0 0.0 3.0666324021606263E-4 6.133264804321253E-5 16-17 6.133264804321253E-5 0.0 0.0 3.0666324021606263E-4 6.133264804321253E-5 18-19 6.133264804321253E-5 0.0 0.0 3.0666324021606263E-4 6.133264804321253E-5 20-21 6.133264804321253E-5 0.0 0.0 3.9866221228088144E-4 6.133264804321253E-5 22-23 6.133264804321253E-5 0.0 0.0 8.893233966265816E-4 6.133264804321253E-5 24-25 1.2266529608642506E-4 0.0 0.0 0.0014106509049938883 6.133264804321253E-5 26-27 1.2266529608642506E-4 0.0 0.0 0.0023613069496636824 6.133264804321253E-5 28-29 1.2266529608642506E-4 0.0 0.0 0.004262619039003271 6.133264804321253E-5 30-31 1.2266529608642506E-4 0.0 0.0 0.010487882815389343 6.133264804321253E-5 32-33 1.2266529608642506E-4 0.0 0.0 0.025606380558041233 6.133264804321253E-5 34-35 1.839979441296376E-4 0.0 0.0 0.04961811226695894 6.133264804321253E-5 36-37 2.4533059217285013E-4 0.0 0.0 0.08917767025483102 6.133264804321253E-5 38-39 3.0666324021606263E-4 0.0 0.0 0.14440771981774392 6.133264804321253E-5 40-41 3.0666324021606263E-4 0.0 0.0 0.201723079414126 6.133264804321253E-5 42-43 3.0666324021606263E-4 0.0 0.0 0.25848644517811925 6.133264804321253E-5 44-45 3.0666324021606263E-4 0.0 0.0 0.3308283035450884 6.133264804321253E-5 46-47 3.679958882592752E-4 0.0 0.0 0.41543669152070006 6.133264804321253E-5 48-49 3.679958882592752E-4 0.0 0.0 0.5072516656413892 1.2266529608642506E-4 50-51 3.679958882592752E-4 0.0 0.0 0.6049239076502052 1.2266529608642506E-4 52-53 3.679958882592752E-4 0.0 0.0 0.6971988766312185 1.2266529608642506E-4 54-55 3.679958882592752E-4 0.0 0.0 0.8026603649415224 1.2266529608642506E-4 56-57 3.679958882592752E-4 0.0 0.0 0.9344948919104077 1.2266529608642506E-4 58-59 3.679958882592752E-4 0.0 0.0 1.0804972605772751 1.2266529608642506E-4 60-61 3.679958882592752E-4 0.0 0.0 1.2365581835232295 1.2266529608642506E-4 62-63 4.293285363024877E-4 0.0 0.0 1.3947350828266747 1.2266529608642506E-4 64-65 4.293285363024877E-4 0.0 0.0 1.5571439348451013 1.2266529608642506E-4 66-67 4.906611843457003E-4 0.0 0.0 1.749483119108616 1.2266529608642506E-4 68-69 4.906611843457003E-4 0.0 0.0 1.9751565975836163 1.2266529608642506E-4 70-71 5.519938323889128E-4 0.0 0.0 2.2340417049740164 1.2266529608642506E-4 72-73 6.133264804321253E-4 0.0 0.0 2.4812429429121847 1.2266529608642506E-4 74-75 6.133264804321253E-4 0.0 0.0 2.7370920842244457 1.2266529608642506E-4 76-77 6.133264804321253E-4 0.0 0.0 3.0307834693793687 1.2266529608642506E-4 78-79 6.133264804321253E-4 0.0 0.0 3.3602931209915283 1.2266529608642506E-4 80-81 6.133264804321253E-4 0.0 0.0 3.7228304035749575 1.2266529608642506E-4 82-83 6.746591284753379E-4 0.0 0.0 4.099382196236261 1.2266529608642506E-4 84-85 7.359917765185504E-4 0.0 0.0 4.473971344160181 1.2266529608642506E-4 86-87 7.973244245617629E-4 0.0 0.0 4.87505619603877 1.2266529608642506E-4 88-89 8.279907485833691E-4 0.0 0.0 5.327292476385397 1.2266529608642506E-4 90-91 0.001165320312821038 0.0 0.0 5.8055951321503905 1.2266529608642506E-4 92-93 0.001165320312821038 0.0 0.0 6.319317392160338 1.2266529608642506E-4 94-95 0.001165320312821038 0.0 0.0 6.835584957064079 1.2266529608642506E-4 96-97 0.001165320312821038 0.0 0.0 7.35890577649279 1.5333162010803132E-4 98-99 0.0011959866368426442 0.0 0.0 7.913444913775496 1.839979441296376E-4 100-101 0.0012266529608642505 0.0 0.0 8.534867303749326 1.839979441296376E-4 102-103 0.0012266529608642505 0.0 0.0 9.17183752000211 1.839979441296376E-4 104-105 0.0013493182569506758 0.0 0.0 9.816075655048014 1.839979441296376E-4 106-107 0.001379984580972282 0.0 0.0 10.462521765423475 1.839979441296376E-4 108-109 0.0014106509049938883 0.0 0.0 11.126386347843207 1.839979441296376E-4 110-111 0.0014106509049938883 0.0 0.0 11.82751051394919 1.839979441296376E-4 112-113 0.0014413172290154946 0.0 0.0 12.582699409305267 1.839979441296376E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1865 0.0 52.00603 1 GTATCAA 2985 0.0 44.652794 1 ATCAACG 3280 0.0 39.904987 3 CAACGCA 3335 0.0 39.06849 5 TCAACGC 3400 0.0 38.321594 4 AACGCAG 3510 0.0 37.101276 6 TATCAAC 3870 0.0 33.82232 2 GTACATG 6760 0.0 31.952501 1 ACGCAGA 4195 0.0 30.900326 7 TACATGG 6960 0.0 30.774067 2 CGCAGAG 4215 0.0 30.612633 8 ACATGGG 7010 0.0 29.365435 3 CATGGGG 3940 0.0 25.97231 4 GCAGAGT 5020 0.0 24.282982 9 CGATCGT 240 0.0 22.302986 24-25 TATTCCG 165 3.2180082E-4 21.63444 5 CAGAGTA 4855 0.0 21.433874 10-11 GAGTACA 5360 0.0 21.20379 1 CATGGGA 3780 0.0 20.461142 4 AGTACAT 5265 0.0 20.22769 2 >>END_MODULE