FastQCFastQC Report
Thu 26 May 2016
SRR1364217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630453
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65260.4002568611300049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61780.378913099610967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34210.20981898895583007No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.10800679320409727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30700.073.133011
GTATCAA58550.052.5864831
ATCAACG72100.042.7548263
CAACGCA74100.041.588095
TCAACGC74150.041.5727964
AACGCAG75650.041.0505526
TATCAAC75800.040.356292
ACGCAGA90350.034.370557
CGCAGAG90550.034.2289358
TGGTATC13850.033.0870632
GTACATG63000.031.194921
GTGGTAT15600.030.9222371
GCAGAGT100300.030.5457219
TACATGG64250.029.548462
ACATGGG64550.027.8420583
AATACCG1107.758848E-427.0417465
GAGTACT71800.026.84672512-13
CAGAGTA99500.026.42672310-11
GTACTTT75950.025.37978714-15
AGAGTAC95750.025.13181510-11
Produced by FastQC (version 0.11.5)