FastQCFastQC Report
Thu 26 May 2016
SRR1364216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049908
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21220.10351684075578026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.054.1361161
GTATCAA34550.044.5961531
ATCAACG35950.041.0371633
CAACGCA36850.040.1973085
TCAACGC37950.039.032174
AACGCAG39500.037.4868056
TATCAAC42200.035.2413142
GTACATG83500.034.3404731
TACATGG85800.032.0316432
ACATGGG85850.030.835023
ACGCAGA49200.029.4910567
CGCAGAG50150.028.5766748
CATGGGG48700.027.1180924
GCAGAGT62050.022.8092679
CATGGGA47050.022.3794164
TGGTATC13250.021.999092
GAGTACA67100.021.2781771
GTGGTAT14800.020.9019781
CAGAGTA56100.020.77635610-11
AGTACAT66800.020.4821662
Produced by FastQC (version 0.11.5)