FastQCFastQC Report
Thu 26 May 2016
SRR1364216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049908
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69490.3389908230027884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64910.3166483569018707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.1754225067661573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.073.1789551
GTATCAA71750.053.022981
ATCAACG91350.041.106953
CAACGCA93600.039.9173475
TATCAAC94350.039.864912
TCAACGC94950.039.4230424
AACGCAG97100.038.9064646
GTGGTAT18100.035.853631
ACGCAGA114200.032.9236267
TGGTATC18500.032.8128552
CGCAGAG115300.032.506338
GTACATG85100.032.042111
TACATGG86850.030.8360212
ACATGGG86350.029.359033
GCAGAGT129700.028.6679579
AACGCGT450.00953027526.44529116-117
GAGTACT87850.025.53032312-13
AGAGTAC120250.025.33006110-11
CAGAGTA126600.024.99938610-11
GTACTTT92000.024.24935214-15
Produced by FastQC (version 0.11.5)