FastQCFastQC Report
Thu 26 May 2016
SRR1364215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190892
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71010.5962757328120435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.063.3951721
GTATCAA37800.047.6945421
GTACATG103050.040.7060551
TACATGG103200.039.3142132
TCAACGC45550.037.875164
ATCAACG45800.037.7983133
CAACGCA47100.036.8798035
ACATGGG105500.036.1451873
AACGCAG48400.035.874166
TATCAAC50600.035.3883972
CATGGGG26750.035.360494
CATGGGA76900.031.7177564
ATGGGAG29300.030.6574465
CGCAGAG56500.029.9944328
ATGGGAT27300.029.6348635
ACGCAGA58250.029.6037227
ATGGGGG13900.027.8179555
GCAGAGT61350.026.9481669
GAGTACA71050.024.5369991
GTCGGGA6550.024.522652
Produced by FastQC (version 0.11.5)