##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364215_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1190892 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.41216415930244 31.0 31.0 34.0 26.0 34.0 2 30.39134363149639 33.0 31.0 34.0 26.0 34.0 3 30.519794406209797 33.0 31.0 34.0 26.0 34.0 4 33.63613241167125 37.0 35.0 37.0 27.0 37.0 5 33.97389267876516 37.0 35.0 37.0 30.0 37.0 6 33.90267967204415 37.0 35.0 37.0 29.0 37.0 7 33.85924500290538 37.0 35.0 37.0 29.0 37.0 8 33.73873197569553 37.0 35.0 37.0 28.0 37.0 9 35.04456239524659 39.0 37.0 39.0 26.0 39.0 10-11 35.11131362037868 39.0 37.0 39.0 27.0 39.0 12-13 35.048028284680726 39.0 37.0 39.0 27.0 39.0 14-15 36.30483536710298 40.0 37.0 41.0 27.0 41.0 16-17 36.38092958891318 40.0 37.0 41.0 27.0 41.0 18-19 36.319795581799184 40.0 37.0 41.0 27.0 41.0 20-21 36.338526079610915 40.0 37.0 41.0 27.0 41.0 22-23 36.446900726514244 40.0 37.0 41.0 27.0 41.0 24-25 36.369508737988 40.0 36.0 41.0 27.0 41.0 26-27 36.44372621530752 40.0 36.0 41.0 28.0 41.0 28-29 36.54889528185595 40.0 36.0 41.0 29.0 41.0 30-31 36.44095224420015 40.0 36.0 41.0 29.0 41.0 32-33 36.2574801073481 40.0 36.0 41.0 28.0 41.0 34-35 36.19633770316703 40.0 36.0 41.0 28.0 41.0 36-37 36.19926576045519 40.0 36.0 41.0 28.0 41.0 38-39 36.01845843283858 40.0 35.5 41.0 27.0 41.0 40-41 36.00674074559238 40.0 35.0 41.0 27.0 41.0 42-43 35.926982043711774 40.0 35.0 41.0 27.0 41.0 44-45 35.924069520997705 40.0 35.0 41.0 27.0 41.0 46-47 35.861127205489666 40.0 35.0 41.0 27.0 41.0 48-49 35.77618793307873 40.0 35.0 41.0 27.0 41.0 50-51 35.678482599597615 40.0 35.0 41.0 27.0 41.0 52-53 35.547422856144806 39.5 35.0 41.0 27.0 41.0 54-55 35.36577918064778 39.0 35.0 41.0 27.0 41.0 56-57 35.19271142975182 39.0 34.5 41.0 27.0 41.0 58-59 34.96796350970533 39.0 34.0 41.0 27.0 41.0 60-61 34.73145717663735 38.0 34.0 40.5 26.5 41.0 62-63 34.44851674207233 38.0 34.0 40.0 26.0 41.0 64-65 34.14291346318558 37.0 34.0 40.0 26.0 41.0 66-67 33.84195922048347 37.0 33.0 40.0 26.0 41.0 68-69 33.60090671530248 36.0 33.0 39.0 25.5 41.0 70-71 33.42148070521928 36.0 33.0 39.0 25.0 41.0 72-73 33.103244458775436 35.5 33.0 39.0 25.0 41.0 74-75 32.75516083742271 35.0 33.0 37.5 24.5 40.0 76-77 32.41835531685493 35.0 33.0 37.0 24.0 39.0 78-79 32.065417351027634 35.0 33.0 37.0 22.5 39.0 80-81 31.75401085908714 35.0 33.0 36.0 21.5 39.0 82-83 31.470805497055988 35.0 32.5 36.0 20.0 37.0 84-85 31.208597001239408 35.0 32.0 35.5 19.5 37.0 86-87 30.982782653674725 35.0 32.0 35.0 18.5 36.5 88-89 30.793936393896338 35.0 32.0 35.0 17.5 36.0 90-91 30.62823622964971 35.0 32.0 35.0 13.5 36.0 92-93 30.487841046879147 35.0 32.0 35.0 9.0 36.0 94-95 30.34819110381126 35.0 32.0 35.0 7.0 35.5 96-97 30.245549134598267 35.0 32.0 35.0 4.5 35.0 98-99 30.15444011715588 35.0 32.0 35.0 2.0 35.0 100-101 30.041524336379787 35.0 31.0 35.0 2.0 35.0 102-103 29.100888661608273 33.5 29.5 35.0 2.0 35.0 104-105 29.728885994699773 34.5 31.0 35.0 2.0 35.0 106-107 29.801973226791347 35.0 31.0 35.0 2.0 35.0 108-109 29.745165808486412 35.0 31.0 35.0 2.0 35.0 110-111 29.661125442105583 35.0 31.0 35.0 2.0 35.0 112-113 29.786456706401587 35.0 31.5 35.0 2.0 35.0 114-115 29.759838843488744 35.0 32.0 35.0 2.0 35.0 116-117 29.656475566214233 35.0 31.5 35.0 2.0 35.0 118-119 29.403837207740082 35.0 31.0 35.0 2.0 35.0 120-121 29.323397503719903 35.0 31.0 35.0 2.0 35.0 122-123 29.169091739637178 35.0 31.0 35.0 2.0 35.0 124-125 28.315577315155362 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7166860707189571 1101 2 0.8415900366009339 1101 3 0.8962008077492101 1101 4 0.6253034855815969 1101 5 0.5830762908504283 1101 6 0.7740507984344802 1101 7 0.6632400907547051 1101 8 0.5941067233685615 1101 9 0.8822660586573932 1101 10-11 0.8029972422417018 1101 12-13 0.7592791395478855 1101 14-15 0.6297970228426095 1101 16-17 0.7940131540928164 1101 18-19 0.9077303245254171 1101 20-21 0.9482048490357258 1101 22-23 0.795425482759434 1101 24-25 1.1066640428539571 1101 26-27 1.0804957707373646 1101 28-29 1.0756641901457087 1101 30-31 1.038249445528301 1101 32-33 1.2787131073798434 1101 34-35 1.1969765369962388 1101 36-37 1.095043427024521 1101 38-39 1.1360619769004572 1101 40-41 0.9753895549376281 1101 42-43 0.9413730831173766 1101 44-45 0.8459820036930594 1101 46-47 0.8296438644805804 1101 48-49 0.8197454093271048 1101 50-51 0.7567858604038307 1101 52-53 0.7963055508868351 1101 54-55 0.7667304197467502 1101 56-57 0.868763520411278 1101 58-59 0.8800387812688015 1101 60-61 0.7778566595278917 1101 62-63 0.7520408750636776 1101 64-65 0.733717531011429 1101 66-67 0.6737758461638776 1101 68-69 0.6423232179302403 1101 70-71 0.6007687707015279 1101 72-73 0.6312519170191777 1101 74-75 0.6675069339513371 1101 76-77 0.679739339635745 1101 78-79 0.6545837406054105 1101 80-81 0.6333751198730475 1101 82-83 0.644593221831876 1101 84-85 0.6108722096461037 1101 86-87 0.6748377855041134 1101 88-89 0.6457121887699238 1101 90-91 0.5960635984701135 1101 92-93 0.6704997276626727 1101 94-95 0.671997357788598 1101 96-97 0.7036431962545429 1101 98-99 0.6066013160446673 1101 100-101 0.6452866645930655 1101 102-103 0.48200021518431413 1101 104-105 0.5780599088718326 1101 106-107 0.4988178155032621 1101 108-109 0.4667245725537619 1101 110-111 0.48630829765027883 1101 112-113 0.482806620426917 1101 114-115 0.540376183333187 1101 116-117 0.5737306344436348 1101 118-119 0.421588955006694 1101 120-121 0.3461519222703906 1101 122-123 0.4594869481283048 1101 124-125 0.3314852128421535 1102 1 0.47301495467170795 1102 2 0.443303973651453 1102 3 0.45827686541976576 1102 4 0.5573280565831524 1102 5 0.40154156305791133 1102 6 0.35425893894989 1102 7 0.2092620428604448 1102 8 0.26811863865893315 1102 9 0.450148935055104 1102 10-11 0.4007588404537188 1102 12-13 0.4461174741495313 1102 14-15 0.48796770354984886 1102 16-17 0.5130717913071905 1102 18-19 0.6731183941523966 1102 20-21 0.5976704434633788 1102 22-23 0.5842129065749262 1102 24-25 0.7153139403605238 1102 26-27 0.7174122608685138 1102 28-29 0.6480324398046307 1102 30-31 0.6825855691511507 1102 32-33 0.8290373842698102 1102 34-35 0.8198658235185263 1102 36-37 0.7346031508749817 1102 38-39 0.8911170628372602 1102 40-41 0.8001480612072029 1102 42-43 0.8049796865740504 1102 44-45 0.6101339819191978 1102 46-47 0.5837463442047124 1102 48-49 0.6045054238612906 1102 50-51 0.6024567982016222 1102 52-53 0.586335579063963 1102 54-55 0.5145619465904332 1102 56-57 0.4904581928093492 1102 58-59 0.45487098683155835 1102 60-61 0.46194172565158453 1102 62-63 0.5003468196829459 1102 64-65 0.5315901923443178 1102 66-67 0.4984939035190692 1102 68-69 0.5451676678592747 1102 70-71 0.4798453699761893 1102 72-73 0.5639414955963318 1102 74-75 0.5825042259307978 1102 76-77 0.4059944557378401 1102 78-79 0.5036523872121563 1102 80-81 0.4911063471205317 1102 82-83 0.4965567081169553 1102 84-85 0.43805799356663044 1102 86-87 0.4588292706648467 1102 88-89 0.3094541932535826 1102 90-91 0.4024360164239482 1102 92-93 0.46592008786368666 1102 94-95 0.5215383654464709 1102 96-97 0.5729530966405214 1102 98-99 0.5479867256413442 1102 100-101 0.5035163047348377 1102 102-103 0.5089025139166843 1102 104-105 0.5541336904339822 1102 106-107 0.49244162688791704 1102 108-109 0.4661150965385943 1102 110-111 0.44233107244580694 1102 112-113 0.4516863226132166 1102 114-115 0.30275732179873316 1102 116-117 0.30994886662040244 1102 118-119 0.22454275411955749 1102 120-121 0.28224160916836993 1102 122-123 0.370844180599871 1102 124-125 0.3062516032221687 1103 1 0.46498407325923097 1103 2 0.1701267615494224 1103 3 0.44217092978021455 1103 4 0.4020533610129604 1103 5 0.4036994380158774 1103 6 0.23834090259632745 1103 7 0.30521198912784087 1103 8 0.23927379072549826 1103 9 0.4151607694136459 1103 10-11 0.2637805906201862 1103 12-13 0.24061532154893683 1103 14-15 0.3386593047814088 1103 16-17 0.33490074362033084 1103 18-19 0.4766881509381946 1103 20-21 0.6725367712520338 1103 22-23 0.48203788992771024 1103 24-25 0.8552280341255596 1103 26-27 0.9080957527624705 1103 28-29 0.7387109869805428 1103 30-31 0.8504541319523682 1103 32-33 1.0489649276679458 1103 34-35 1.0018759503010983 1103 36-37 0.8126314610340728 1103 38-39 1.013712116800619 1103 40-41 0.8684164868749136 1103 42-43 0.8452873798960425 1103 44-45 0.7813811057415379 1103 46-47 0.6570165170061202 1103 48-49 0.7021995370096263 1103 50-51 0.7185413067296125 1103 52-53 0.6470230926619394 1103 54-55 0.7430339627827323 1103 56-57 0.7456880562692021 1103 58-59 0.6584334913642138 1103 60-61 0.5787883800553502 1103 62-63 0.6366258863838965 1103 64-65 0.6805372970470742 1103 66-67 0.6309652076719701 1103 68-69 0.6449659233627472 1103 70-71 0.6922325479926315 1103 72-73 0.7108945603325836 1103 74-75 0.7086406869631148 1103 76-77 0.722713536415867 1103 78-79 0.6562328707396574 1103 80-81 0.6470429756139673 1103 82-83 0.5392259987806653 1103 84-85 0.5118252753562231 1103 86-87 0.6092416601503956 1103 88-89 0.6124427808443151 1103 90-91 0.6317153402502349 1103 92-93 0.6563339277998637 1103 94-95 0.7174635881236036 1103 96-97 0.7379378998737103 1103 98-99 0.7503859028402644 1103 100-101 0.7034803731169958 1103 102-103 0.452716012337671 1103 104-105 0.5089870960261287 1103 106-107 0.44252931322137457 1103 108-109 0.4110777474349092 1103 110-111 0.46014260616548697 1103 112-113 0.4291611539983293 1103 114-115 0.448166315911223 1103 116-117 0.4285017784000402 1103 118-119 0.3555613001087359 1103 120-121 0.4147129133212708 1103 122-123 0.437308113826397 1103 124-125 0.4399739210422702 1104 1 0.46390237395335987 1104 2 0.5077086642043191 1104 3 0.4898822250841235 1104 4 0.4993628869518716 1104 5 0.40373175190582344 1104 6 0.5222086336847056 1104 7 0.3725117033947214 1104 8 0.4970841232060508 1104 9 0.4244887123114083 1104 10-11 0.5458488623623765 1104 12-13 0.5908316287698199 1104 14-15 0.508692382454214 1104 16-17 0.6991804474292636 1104 18-19 0.8181364594855793 1104 20-21 0.7591934848053938 1104 22-23 0.7076079363226242 1104 24-25 0.9563481510561402 1104 26-27 0.8526687818273544 1104 28-29 0.7207487074085961 1104 30-31 0.7733551489586148 1104 32-33 0.877532560639608 1104 34-35 0.7613879844506783 1104 36-37 0.5868774865888113 1104 38-39 0.7139467678660978 1104 40-41 0.7580441525260255 1104 42-43 0.571600808961179 1104 44-45 0.5649596438350457 1104 46-47 0.5188911208345672 1104 48-49 0.39997588441723764 1104 50-51 0.3526230602183702 1104 52-53 0.535083534650667 1104 54-55 0.47563750268778193 1104 56-57 0.5096951483520371 1104 58-59 0.5731083385586899 1104 60-61 0.6480788257846584 1104 62-63 0.6983591251038987 1104 64-65 0.548657784118582 1104 66-67 0.5558148501628395 1104 68-69 0.5546197143042804 1104 70-71 0.5507022796698422 1104 72-73 0.5320745526164146 1104 74-75 0.5165302047941864 1104 76-77 0.5340128224420937 1104 78-79 0.5557208424653268 1104 80-81 0.5301076575767532 1104 82-83 0.5940009798656121 1104 84-85 0.650860846094595 1104 86-87 0.5954565220591093 1104 88-89 0.6604741142198627 1104 90-91 0.6537986199990868 1104 92-93 0.7302196371615572 1104 94-95 0.6830016405034449 1104 96-97 0.7152845574097988 1104 98-99 0.6656849956359636 1104 100-101 0.6856060591343507 1104 102-103 0.6458047240840656 1104 104-105 0.6887011213641365 1104 106-107 0.6100701413317147 1104 108-109 0.7620508198460954 1104 110-111 0.693499647231544 1104 112-113 0.7874490792442259 1104 114-115 0.8461241107069242 1104 116-117 0.7609913057620687 1104 118-119 0.678041028854846 1104 120-121 0.7047912761364081 1104 122-123 0.7200309866421897 1104 124-125 0.6890939387463639 1105 1 0.20629658968154274 1105 2 0.251680444871603 1105 3 0.360810754677928 1105 4 0.20329323742657124 1105 5 0.26400656597852645 1105 6 0.1479906850775521 1105 7 0.18406548852013316 1105 8 0.13374126775367756 1105 9 0.153630850712986 1105 10-11 0.29671193597476275 1105 12-13 0.16452711286552812 1105 14-15 0.26369299074410435 1105 16-17 0.17019709595802368 1105 18-19 0.31549289143655557 1105 20-21 0.406457144237514 1105 22-23 0.4874108171569702 1105 24-25 0.6408901752454739 1105 26-27 0.658650066442533 1105 28-29 0.583555902115215 1105 30-31 0.6254095732870226 1105 32-33 0.8260670805925088 1105 34-35 0.7172211521385776 1105 36-37 0.6328299655197114 1105 38-39 0.711146806158446 1105 40-41 0.7366027647475377 1105 42-43 0.6075071974676263 1105 44-45 0.5080365070801705 1105 46-47 0.5435572760502012 1105 48-49 0.5499156869856208 1105 50-51 0.5138901845760913 1105 52-53 0.5655719881118415 1105 54-55 0.558996319932902 1105 56-57 0.6046309189223749 1105 58-59 0.677494374615982 1105 60-61 0.5362201311947175 1105 62-63 0.5338597543349266 1105 64-65 0.6144639477239693 1105 66-67 0.5373871565521497 1105 68-69 0.5248008783251663 1105 70-71 0.6366406781854792 1105 72-73 0.5784922237370083 1105 74-75 0.5319204377193572 1105 76-77 0.6157704744298087 1105 78-79 0.5372855036810904 1105 80-81 0.4008743089309128 1105 82-83 0.3160731609405545 1105 84-85 0.37200264279015016 1105 86-87 0.46739172156822306 1105 88-89 0.4694719183930651 1105 90-91 0.3633262346982953 1105 92-93 0.3194775367864118 1105 94-95 0.34589868261904044 1105 96-97 0.3848598248080499 1105 98-99 0.39673992321622364 1105 100-101 0.3801627620568695 1105 102-103 0.2739212125960293 1105 104-105 0.32305158409657153 1105 106-107 0.4163668724549545 1105 108-109 0.3646121524197845 1105 110-111 0.34146932181971934 1105 112-113 0.35023406970769955 1105 114-115 0.3486687900982588 1105 116-117 0.43963108978259413 1105 118-119 0.3897054695572173 1105 120-121 0.39547470146666086 1105 122-123 0.39449717071004997 1105 124-125 0.3927106709066841 1106 1 0.3467205802379709 1106 2 0.4261291116137329 1106 3 0.2168140534602898 1106 4 0.26003294085956696 1106 5 0.3719097370139224 1106 6 0.24500269976037004 1106 7 0.22170912300211398 1106 8 0.2989892423983349 1106 9 0.33318163417379765 1106 10-11 0.2610100553454089 1106 12-13 0.2716812662589092 1106 14-15 0.2867066871591035 1106 16-17 0.2834819716877277 1106 18-19 0.48915825907291577 1106 20-21 0.48038026967208936 1106 22-23 0.5913449385327567 1106 24-25 0.8046360719806458 1106 26-27 0.6706755834943294 1106 28-29 0.6551331728579228 1106 30-31 0.7223648295086207 1106 32-33 0.6921302102314257 1106 34-35 0.6786253985123452 1106 36-37 0.6951352322521345 1106 38-39 0.7176041726305229 1106 40-41 0.5777495767331118 1106 42-43 0.6104495819698172 1106 44-45 0.5392625545244201 1106 46-47 0.5569750733044572 1106 48-49 0.5410600733959257 1106 50-51 0.6028019098069279 1106 52-53 0.5319594980162776 1106 54-55 0.4697081454208387 1106 56-57 0.558658505798391 1106 58-59 0.42651088469829546 1106 60-61 0.47661679360314935 1106 62-63 0.404451918109757 1106 64-65 0.3783647578661018 1106 66-67 0.3768721260707508 1106 68-69 0.40452274569471314 1106 70-71 0.5034093268166444 1106 72-73 0.3532298573457737 1106 74-75 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2216 106-107 -2.4462804911024953 2216 108-109 -2.4230478122969714 2216 110-111 -2.4661599800760534 2216 112-113 -2.491617880671736 2216 114-115 -2.4990041958164433 2216 116-117 -2.378247155015927 2216 118-119 -2.2619564833424484 2216 120-121 -2.176491919462464 2216 122-123 -2.2282136186825774 2216 124-125 -2.2441099165873624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26037.0 3 5913.0 4 5172.0 5 4170.0 6 4547.0 7 4487.0 8 4003.0 9 3792.0 10 4390.0 11 5325.0 12 5550.0 13 5514.0 14 5516.0 15 5478.0 16 5083.0 17 4471.0 18 3894.0 19 3439.0 20 3262.0 21 3248.0 22 3718.0 23 5621.0 24 10540.0 25 14978.0 26 18242.0 27 13648.0 28 13976.0 29 16895.0 30 19728.0 31 23588.0 32 30892.0 33 40969.0 34 59509.0 35 94057.0 36 181863.0 37 356037.0 38 172486.0 39 854.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.330464176572455 19.780208700065288 12.34025891310942 22.549068210252837 2 15.481600408646434 22.41111477974344 39.0907616836838 23.01652312792633 3 18.479444321229106 28.773917777284918 28.578593463314313 24.168044438171663 4 11.754936009556982 18.539082307990867 36.46127893515602 33.24470274729613 5 12.541531937272179 42.07303908349568 32.271459882773875 13.113969096458266 6 29.736231974116116 38.38941199489108 17.918188329917026 13.956167701075781 7 27.335270260057605 36.12735013309374 20.123689005701618 16.413690601147042 8 25.495175045260194 37.83097039865916 19.294192924295402 17.37966163178525 9 26.810733194221104 18.951368100194394 18.81432716005324 35.42357154553126 10-11 23.733725323730074 30.403146335943664 26.32150854162022 19.541619798706044 12-13 24.149066059378537 28.37804021940299 26.30392285291065 21.168970868307824 14-15 21.90101851824637 28.8895224506917 24.503431940836336 24.706027090225593 16-17 21.674969507266823 31.91616125355049 25.0312484911083 21.377620748074392 18-19 21.689844924981717 30.341993025109105 26.936296388356816 21.031865661552363 20-21 23.358774651394317 29.12361188616364 26.448851361650078 21.06876210079196 22-23 22.969358231956967 29.440116906512582 26.013756671523776 21.576768190006675 24-25 22.17373250160913 29.331797747761172 26.754163694373684 21.740306056256006 26-27 22.11219445213127 29.544107945724797 26.553076128140408 21.790621474003522 28-29 21.82634206313116 29.567398229687193 26.70878937929882 21.897470327882825 30-31 22.070481437005064 29.21200695325031 26.933053971666343 21.784457638078283 32-33 21.807482198886273 29.438774203489643 26.64387653863804 22.10986705898604 34-35 22.17559640039137 29.244636490759525 26.760142271045655 21.81962483780345 36-37 22.40348700247542 28.999481400763834 26.43130239794408 22.16572919881667 38-39 22.004676806466893 29.27707726474017 26.66514636858745 22.05309956020548 40-41 22.196045170459914 29.127556793728704 26.8173888215918 21.859009214219583 42-43 22.072846161177516 29.072378859489984 26.668777151676554 22.185997827655946 44-45 21.89833212625038 29.28408434885747 26.669899549705576 22.14768397518657 46-47 22.12537446284132 29.236757083566882 26.502417345265762 22.135451108326038 48-49 21.934171998243286 29.302076396825488 26.52018161649428 22.24356998843694 50-51 21.845701689933872 29.312186417550123 26.577222630418813 22.2648892620972 52-53 22.147184089695315 29.266649088570095 26.500911933736177 22.085254887998413 54-55 21.76073561674032 29.059212528997445 26.802901319449546 22.377150534812685 56-57 21.936752670014094 29.428411040886683 26.395194415485555 22.239641873613664 58-59 22.037635995587845 29.13331902924207 26.395961039686295 22.43308393548379 60-61 21.853908639886118 29.36049599929455 26.595883608970144 22.189711751849185 62-63 21.86255116004765 29.266801511822187 26.61611582966512 22.25453149846504 64-65 21.570088733521487 29.1891047629924 26.670733724700195 22.570072778785914 66-67 21.750317211195057 29.490606265960277 26.191819771977425 22.56725675086724 68-69 21.802168732876353 29.149659364076292 26.62985629257949 22.41831561046786 70-71 21.50980565864325 29.160314509956386 26.851033510416976 22.478846320983383 72-73 21.826798207513146 29.274234282045867 26.58613379664016 22.31283371380083 74-75 21.496360263770665 29.50042111609621 26.581914428201596 22.421304191931533 76-77 21.661497561276676 29.316531886459657 26.659190462255317 22.36278009000835 78-79 21.841699542012424 29.39521959723293 26.48686413707046 22.27621672368419 80-81 21.68804603029051 29.352203602750265 26.64909125108744 22.310659115871783 82-83 21.721164181109767 29.37491602848314 26.615402055622734 22.28851773478436 84-85 21.821435634688324 29.108656209283158 26.56147978261116 22.50842837341736 86-87 21.782818014858705 28.95474283622085 26.658084323619835 22.604354825300614 88-89 21.92415499178756 28.891463091035508 26.6731800792022 22.511201837974735 90-91 21.838767739440726 28.951464226799494 26.811878062062274 22.397889971697506 92-93 21.837516836458786 28.898207022104884 26.707578001928006 22.556698139508324 94-95 21.645758660633653 28.67326704405901 26.826499307451602 22.854474987855735 96-97 21.873416339700512 28.631780480278113 26.7070992066741 22.787703973347273 98-99 21.508730980966583 28.685937075456287 26.63876235546879 23.166569588108345 100-101 21.292579279763505 29.038900833109377 26.81604407417361 22.852475812953507 102-103 21.55976553971213 29.036882148435534 26.702482323105087 22.70086998874725 104-105 21.29866487059373 29.030036632497612 26.822764888565654 22.848533608343004 106-107 21.041888820898087 29.45598164836305 26.77950526681397 22.722624263924896 108-109 21.29164093290637 29.609394057590162 26.2292480227865 22.86971698671697 110-111 21.10378364171264 29.864305828522102 26.355881571262845 22.676028958502414 112-113 21.206834070918163 29.91209870073372 26.432285597548688 22.448781630799424 114-115 21.075211103635443 29.987089772697683 26.41225035257436 22.525448771092517 116-117 21.01350866013532 29.994904690834872 26.452425648601775 22.53916100042804 118-119 21.103234729197517 30.00187943151832 26.257175340847127 22.637710498437038 120-121 21.018756478513403 30.068751056108024 26.397068033953502 22.515424431425064 122-123 21.147359036713258 29.957624823261792 26.301920719659922 22.593095420365028 124-125 21.157331686673555 30.011638099337894 26.27097690534388 22.56005330864467 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5581.0 1 4541.0 2 3205.5 3 2411.0 4 1588.0 5 1147.5 6 925.0 7 846.0 8 868.5 9 861.5 10 755.5 11 661.0 12 675.5 13 674.0 14 678.5 15 734.5 16 821.5 17 914.5 18 1118.0 19 1363.5 20 1666.0 21 2129.0 22 2646.0 23 3245.5 24 4151.0 25 5477.0 26 7363.5 27 9290.0 28 10953.5 29 13029.0 30 15387.0 31 17837.5 32 20980.5 33 24476.5 34 27979.0 35 30308.5 36 32888.0 37 35942.0 38 36306.0 39 35548.0 40 36005.0 41 37484.5 42 38220.0 43 38685.0 44 39445.0 45 40067.0 46 39976.5 47 39530.0 48 40366.0 49 41279.5 50 40590.0 51 38710.5 52 37036.5 53 36294.5 54 35407.0 55 33638.0 56 31433.0 57 29197.0 58 27653.5 59 25394.0 60 22007.0 61 18360.5 62 14928.5 63 11361.0 64 8322.0 65 6392.0 66 4657.0 67 3602.0 68 2632.5 69 1618.0 70 1133.5 71 917.0 72 817.5 73 785.0 74 598.0 75 432.0 76 311.0 77 162.0 78 82.5 79 41.5 80 28.5 81 18.5 82 15.5 83 11.0 84 9.0 85 9.0 86 7.0 87 5.0 88 6.0 89 6.0 90 3.5 91 1.5 92 2.5 93 2.5 94 2.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06406962176251078 2 0.052145786519684406 3 0.047359458288409026 4 0.04685563426406425 5 0.0455960742032023 6 0.0029389734753445316 7 1.6794134144825895E-4 8 0.0 9 5.877946950689063E-4 10-11 0.0039046361886720207 12-13 0.0020152960973791076 14-15 0.007851257712706106 16-17 0.002896988139982467 18-19 0.004114562865482344 20-21 0.009446700456464566 22-23 0.016542222132653505 24-25 0.002729046798534208 26-27 0.015324647407153629 28-29 0.0071794923469130694 30-31 0.006885594999378617 32-33 0.0021412521034653016 34-35 0.0173819288398948 36-37 0.015492588748601887 38-39 0.0282141453633075 40-41 0.005835961615326999 42-43 0.0010496333840516183 44-45 3.7786801825858264E-4 46-47 0.0016374280791205248 48-49 0.002603090792448014 50-51 0.001427501402310201 52-53 0.0013435307315860716 54-55 0.004576401554465056 56-57 0.0023091934449135606 58-59 0.007599345700533718 60-61 0.0138131753341193 62-63 0.01204979124891258 64-65 0.001931325426654978 66-67 0.0037786801825858266 68-69 0.00877493509067153 70-71 0.0019733107620170426 72-73 0.002729046798534208 74-75 7.557360365171653E-4 76-77 2.099266768103237E-4 78-79 0.002519120121723884 80-81 0.0020152960973791076 82-83 0.0010076480486895538 84-85 0.01028640716370586 86-87 0.006297800304309711 88-89 0.0013435307315860716 90-91 6.29780030430971E-4 92-93 0.0020152960973791076 94-95 4.1985335362064737E-5 96-97 0.0016374280791205248 98-99 0.02313391978449767 100-101 0.014442955364550269 102-103 0.006045888292137322 104-105 0.012259717925722904 106-107 0.0311951041740141 108-109 9.65662713327489E-4 110-111 0.001301545396224007 112-113 0.015240676736429499 114-115 0.030481353472858998 116-117 0.048828945026081295 118-119 0.3663640363693769 120-121 0.11613143761147107 122-123 0.01834759155322229 124-125 0.3226573022574675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1190892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.23791168114576 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80962356655726 55.513744438316934 2 9.577164838124936 12.687427974069887 3 2.9075058174693793 5.777613406498629 4 1.2664764465555791 3.3555502005279916 5 0.6638430505901478 2.1985788677566296 6 0.40061392060462503 1.592149769474802 7 0.2404871311581754 1.115055574787514 8 0.17772492874568416 0.9417702507035641 9 0.12926604691797597 0.7706081699211363 >10 0.7432885953669811 9.147861566878932 >50 0.05711438485510017 2.615092089243023 >100 0.026415321318704228 3.0066392948816705 >500 0.0 0.0 >1k 2.37975867729842E-4 0.15857602655986902 >5k 2.37975867729842E-4 1.1193323703793536 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7101 0.5962757328120435 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.6794134144825895E-4 0.0 0.0 0.0 0.0 3 1.6794134144825895E-4 0.0 0.0 0.0 0.0 4 1.6794134144825895E-4 0.0 0.0 0.0 0.0 5 2.5191201217238845E-4 0.0 0.0 0.0 0.0 6 3.358826828965179E-4 0.0 0.0 0.0 0.0 7 3.358826828965179E-4 0.0 0.0 8.397067072412947E-5 0.0 8 4.198533536206474E-4 0.0 0.0 8.397067072412947E-5 0.0 9 4.198533536206474E-4 0.0 0.0 8.397067072412947E-5 0.0 10-11 4.198533536206474E-4 0.0 0.0 8.397067072412947E-5 0.0 12-13 4.198533536206474E-4 0.0 0.0 8.397067072412947E-5 0.0 14-15 4.198533536206474E-4 0.0 0.0 8.397067072412947E-5 0.0 16-17 5.038240243447769E-4 0.0 0.0 8.397067072412947E-5 0.0 18-19 5.038240243447769E-4 0.0 0.0 1.6794134144825895E-4 0.0 20-21 5.038240243447769E-4 0.0 0.0 3.7786801825858264E-4 0.0 22-23 5.038240243447769E-4 0.0 0.0 5.458093597068415E-4 0.0 24-25 6.717653657930358E-4 0.0 0.0 0.0012175747254998773 0.0 26-27 6.717653657930358E-4 0.0 0.0 0.002813017469258337 0.0 28-29 9.65662713327489E-4 0.0 0.0 0.005919932286051128 0.0 30-31 0.0010916187194136833 0.0 0.0 0.017130016827722413 0.0 32-33 0.001133604054775748 0.0 0.0 0.037115036460065226 0.0 34-35 0.0013015453962240068 0.0 0.0 0.0695277153595792 0.0 36-37 0.0015114720730343306 0.0 0.0 0.11818871904421224 0.0 38-39 0.0016794134144825896 0.0 0.0 0.19031952519623946 0.0 40-41 0.0017213987498446543 0.0 0.0 0.2751718879629723 0.0 42-43 0.001763384085206719 0.0 0.0 0.35439821579118846 0.0 44-45 0.001763384085206719 0.0 0.0 0.4467239682523688 0.0 46-47 0.0020152960973791076 0.0 0.0 0.5604622417482021 0.0 48-49 0.002351178780275625 0.0 0.0 0.6883915585964134 0.0 50-51 0.002351178780275625 0.0 0.0 0.8309737574859852 0.0 52-53 0.002351178780275625 0.0 0.0 0.9634794758886616 0.0 54-55 0.002351178780275625 0.0 0.0 1.1074471908451815 0.0 56-57 0.002351178780275625 0.0 0.0 1.2753045616227165 0.0 58-59 0.002351178780275625 0.0 0.0 1.4693607816661796 0.0 60-61 0.0024351494509997546 0.0 0.0 1.696585416645674 0.0 62-63 0.002519120121723884 0.0 0.0 1.9243558609848752 0.0 64-65 0.002519120121723884 0.0 0.0 2.145240710324698 0.0 66-67 0.002687061463172143 0.0 0.0 2.400217651978517 0.0 68-69 0.0027710321338962726 0.0 0.0 2.7016303745427797 0.0 70-71 0.0027710321338962726 0.0 0.0 3.046120051188521 0.0 72-73 0.002855002804620402 0.0 0.0 3.3933807599681582 0.0 74-75 0.0029389734753445316 0.0 0.0 3.735099404480003 0.0 76-77 0.0029389734753445316 0.0 0.0 4.096383215270571 0.0 78-79 0.002980958810706596 0.0 0.0 4.522072530506545 0.0 80-81 0.0034427974996893086 0.0 0.0 5.002804620402186 0.0 82-83 0.0034427974996893086 0.0 0.0 5.516369242550962 0.0 84-85 0.003526768170413438 0.0 0.0 6.00679994491524 0.0 86-87 0.0036107388411375676 0.0 0.0 6.494459615145622 0.0 88-89 0.0036107388411375676 0.0 0.0 7.037581913389292 0.0 90-91 0.003946621524034085 0.0 0.0 7.64960214696211 0.0 92-93 0.00398860685939615 0.0 0.0 8.314188020408231 0.0 94-95 0.004030592194758215 0.0 0.0 8.957277402148978 0.0 96-97 0.004030592194758215 0.0 0.0 9.596336191694965 0.0 98-99 0.004198533536206474 0.0 0.0 10.267723689469742 0.0 100-101 0.004240518871568538 0.0 0.0 11.025978846108632 0.0 102-103 0.004282504206930603 0.0 0.0 11.83117360768231 0.0 104-105 0.004450445548378862 0.0 0.0 12.621883428556075 0.0 106-107 0.004450445548378862 0.0 0.0 13.397394558028772 0.0 108-109 0.004450445548378862 0.0 0.0 14.161023837594005 0.0 110-111 0.004450445548378862 0.0 0.0 14.995650319256491 0.0 112-113 0.004492430883740927 0.0 0.0 15.916304753075845 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2215 0.0 63.395172 1 GTATCAA 3780 0.0 47.694542 1 GTACATG 10305 0.0 40.706055 1 TACATGG 10320 0.0 39.314213 2 TCAACGC 4555 0.0 37.87516 4 ATCAACG 4580 0.0 37.798313 3 CAACGCA 4710 0.0 36.879803 5 ACATGGG 10550 0.0 36.145187 3 AACGCAG 4840 0.0 35.87416 6 TATCAAC 5060 0.0 35.388397 2 CATGGGG 2675 0.0 35.36049 4 CATGGGA 7690 0.0 31.717756 4 ATGGGAG 2930 0.0 30.657446 5 CGCAGAG 5650 0.0 29.994432 8 ATGGGAT 2730 0.0 29.634863 5 ACGCAGA 5825 0.0 29.603722 7 ATGGGGG 1390 0.0 27.817955 5 GCAGAGT 6135 0.0 26.948166 9 GAGTACA 7105 0.0 24.536999 1 GTCGGGA 655 0.0 24.52265 2 >>END_MODULE