FastQCFastQC Report
Thu 26 May 2016
SRR1364215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190892
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121431.0196558546031043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108000.9068832438205985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68490.5751151237895628No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28230.2370492034542175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.19246077729970476No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.12948277425660765No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.11436805352626435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48700.080.5643541
GTATCAA89650.054.19091
CGTATAG853.4661716E-642.0260731
TGGTATC16450.040.8839722
ATCAACG118800.040.4744263
TCAACGC119800.040.2359244
CAACGCA120850.039.7761575
TATCAAC122350.039.2563632
AACGCAG124300.039.0071836
GTGGTAT18500.037.0094451
ACGCAGA142200.034.0118947
CGCAGAG143450.033.632588
GTACATG92250.033.4310381
TACATGG94200.032.601632
ACATGGG93500.031.568643
GCAGAGT152400.031.5403339
GAGTACT104550.030.35767712-13
ATGGGAT26000.029.0595255
CATGGGA70500.028.8684024
GTACTTT111200.028.4887214-15
Produced by FastQC (version 0.11.5)