##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364215_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1190892 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35650084138612 33.0 31.0 34.0 30.0 34.0 2 32.300182552238155 34.0 31.0 34.0 30.0 34.0 3 32.44734954974926 34.0 31.0 34.0 30.0 34.0 4 36.049238721899215 37.0 35.0 37.0 35.0 37.0 5 36.01115130507216 37.0 35.0 37.0 35.0 37.0 6 36.086485592312314 37.0 35.0 37.0 35.0 37.0 7 36.036098151637596 37.0 35.0 37.0 35.0 37.0 8 36.04464384679719 37.0 35.0 37.0 35.0 37.0 9 37.74484084199071 39.0 38.0 39.0 35.0 39.0 10-11 37.73224398182203 39.0 38.0 39.0 35.0 39.0 12-13 37.68247078660366 39.0 37.5 39.0 35.0 39.0 14-15 39.01969112228481 41.0 38.0 41.0 35.0 41.0 16-17 39.058312592577664 40.5 38.0 41.0 35.5 41.0 18-19 39.16997595079991 41.0 39.0 41.0 36.0 41.0 20-21 39.21153975339493 41.0 39.0 41.0 36.0 41.0 22-23 39.15123873533452 41.0 39.0 41.0 35.0 41.0 24-25 39.093920775351584 41.0 39.0 41.0 35.0 41.0 26-27 38.97119260184803 41.0 38.5 41.0 35.0 41.0 28-29 38.859090077017896 40.5 38.0 41.0 35.0 41.0 30-31 38.62498068674573 40.0 38.0 41.0 35.0 41.0 32-33 38.33319226260652 40.0 37.5 41.0 34.0 41.0 34-35 38.18083461808459 40.0 37.0 41.0 34.0 41.0 36-37 38.01858564840472 40.0 37.0 41.0 34.0 41.0 38-39 37.873512879421476 40.0 37.0 41.0 33.0 41.0 40-41 37.69490642308455 40.0 37.0 41.0 33.0 41.0 42-43 37.47146676608794 40.0 37.0 41.0 33.0 41.0 44-45 37.15147763189273 40.0 36.0 41.0 32.5 41.0 46-47 36.68021869321483 40.0 36.0 41.0 30.5 41.0 48-49 36.215055185524804 40.0 36.0 41.0 29.5 41.0 50-51 35.97281029681953 40.0 35.5 41.0 28.0 41.0 52-53 35.819762413384254 40.0 35.0 41.0 28.0 41.0 54-55 35.626349408678536 39.5 35.0 41.0 27.5 41.0 56-57 35.439314816121026 39.0 35.0 41.0 27.0 41.0 58-59 35.22620942957044 39.0 35.0 41.0 26.5 41.0 60-61 35.000861119228276 39.0 35.0 41.0 26.0 41.0 62-63 34.750776728704196 38.0 35.0 40.5 26.0 41.0 64-65 34.46012400788652 37.5 35.0 40.0 26.0 41.0 66-67 34.165286188839964 37.0 34.0 40.0 26.0 41.0 68-69 33.82726267369333 36.5 34.0 39.5 25.0 41.0 70-71 33.4914534651337 36.0 34.0 39.0 24.5 41.0 72-73 33.16128414667325 35.5 34.0 39.0 24.0 40.5 74-75 33.01617778942171 35.0 34.0 38.5 24.5 40.0 76-77 32.72944355995338 35.0 34.0 37.0 24.0 39.0 78-79 32.4243764337992 35.0 34.0 37.0 23.5 39.0 80-81 32.129525179445324 35.0 34.0 36.0 23.0 39.0 82-83 31.856276219841934 35.0 33.5 36.0 22.0 37.0 84-85 31.62902597380787 35.0 33.0 36.0 21.0 37.0 86-87 31.43770341894983 35.0 33.0 35.0 20.0 37.0 88-89 31.267335325117642 35.0 33.0 35.0 20.0 36.0 90-91 31.129250175498704 35.0 33.0 35.0 19.5 36.0 92-93 31.00170208549558 35.0 33.0 35.0 19.0 36.0 94-95 30.90658976632642 35.0 33.0 35.0 18.0 36.0 96-97 30.82351590236562 35.0 33.0 35.0 18.0 35.0 98-99 30.74285073709455 35.0 33.0 35.0 17.0 35.0 100-101 30.66931930015484 35.0 33.0 35.0 14.0 35.0 102-103 29.771014919908772 34.0 30.5 35.0 10.0 35.0 104-105 30.430416863997742 35.0 32.5 35.0 10.0 35.0 106-107 30.52200367455655 35.0 33.0 35.0 7.0 35.0 108-109 30.502370911887894 35.0 33.0 35.0 7.0 35.0 110-111 30.43978589158379 35.0 33.0 35.0 2.0 35.0 112-113 30.524813753052335 35.0 33.0 35.0 2.0 35.0 114-115 30.49057933045146 35.0 33.0 35.0 2.0 35.0 116-117 30.4255759548305 35.0 33.0 35.0 2.0 35.0 118-119 30.330498063636334 35.0 33.0 35.0 2.0 35.0 120-121 30.211429751816283 35.0 33.0 35.0 2.0 35.0 122-123 30.070953117495122 35.0 33.0 35.0 2.0 35.0 124-125 29.40921259022649 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18020969651523444 1101 2 0.33061711467370714 1101 3 0.26552875316814095 1101 4 0.10988538089920752 1101 5 0.1632652694275336 1101 6 0.1448460087749126 1101 7 0.2315447030593134 1101 8 0.1350808859105257 1101 9 0.19744324343514563 1101 10-11 0.18375910716827804 1101 12-13 0.21005338886801184 1101 14-15 0.23616751945084502 1101 16-17 0.20027590739884005 1101 18-19 0.22634407404794388 1101 20-21 0.2099938257460039 1101 22-23 0.1593139005581179 1101 24-25 0.2279401036084181 1101 26-27 0.25372689673397986 1101 28-29 0.26714942432477784 1101 30-31 0.16072790434932926 1101 32-33 0.3086886642410107 1101 34-35 0.2181765249453349 1101 36-37 0.15750761592300933 1101 38-39 0.26081758603765337 1101 40-41 0.3230255534150217 1101 42-43 0.2800355816959268 1101 44-45 0.36360398182335985 1101 46-47 0.45409242344434375 1101 48-49 0.4585690432832408 1101 50-51 0.4010403845790407 1101 52-53 0.44551024201060585 1101 54-55 0.37639848887440763 1101 56-57 0.36819723310609476 1101 58-59 0.383847136058435 1101 60-61 0.38689348866612505 1101 62-63 0.3592289324656477 1101 64-65 0.36114111688838335 1101 66-67 0.217934911479702 1101 68-69 0.10404488744604379 1101 70-71 0.2141589301878284 1101 72-73 0.3355021124743516 1101 74-75 0.17891178208999037 1101 76-77 0.15996378961299484 1101 78-79 0.1834826510853702 1101 80-81 0.20837513276990194 1101 82-83 0.21280086520148345 1101 84-85 0.19957400071128717 1101 86-87 0.06831932398851848 1101 88-89 0.23975047675333983 1101 90-91 0.33685951124471814 1101 92-93 0.26983985051948167 1101 94-95 0.2391548384387434 1101 96-97 0.18151530952887995 1101 98-99 0.11508309742391631 1101 100-101 0.22565113258206537 1101 102-103 0.2359512510142885 1101 104-105 0.27300657167650755 1101 106-107 0.4281492474909676 1101 108-109 0.3199235476795259 1101 110-111 0.2938295002397062 1101 112-113 0.3875677826042292 1101 114-115 0.44532783513886187 1101 116-117 0.31094894325757494 1101 118-119 0.34108377430439774 1101 120-121 0.5028446714477717 1101 122-123 0.4877073248342114 1101 124-125 0.530468787567635 1102 1 0.1829855889622536 1102 2 0.21855785358624757 1102 3 0.1679111256683612 1102 4 0.16912864341266243 1102 5 0.15639512415422985 1102 6 0.14777478440684177 1102 7 0.16362000659756148 1102 8 0.1626930973692282 1102 9 0.27532385743595 1102 10-11 0.170163130121324 1102 12-13 0.24828515429173592 1102 14-15 0.28551485222200057 1102 16-17 0.2578014622002698 1102 18-19 0.2817793112225573 1102 20-21 0.25866689568149326 1102 22-23 0.24185608054892782 1102 24-25 0.21686429960054454 1102 26-27 0.24315327628191596 1102 28-29 0.2103197279658673 1102 30-31 0.2576119667914796 1102 32-33 0.3328277536605455 1102 34-35 0.32592900501225586 1102 36-37 0.37658270693306406 1102 38-39 0.42146867644440533 1102 40-41 0.37840285951732255 1102 42-43 0.24890157238581878 1102 44-45 0.20290490117919546 1102 46-47 0.28375141852877306 1102 48-49 0.3960185678370962 1102 50-51 0.3526436012961014 1102 52-53 0.48038680426422076 1102 54-55 0.4025168327998685 1102 56-57 0.35879765953362863 1102 58-59 0.40182325063169344 1102 60-61 0.4282230239149598 1102 62-63 0.41988962091988924 1102 64-65 0.3873681244228564 1102 66-67 0.3404810678044683 1102 68-69 0.30897177089146766 1102 70-71 0.31992255770131095 1102 72-73 0.29175318368501024 1102 74-75 0.21568601544594657 1102 76-77 0.19427372427772127 1102 78-79 0.23862411944952555 1102 80-81 0.1703020779170572 1102 82-83 0.20783593235353592 1102 84-85 0.21533570861527807 1102 86-87 0.2199071152721146 1102 88-89 0.1352109430813293 1102 90-91 0.10725536214590647 1102 92-93 0.05852923713569069 1102 94-95 0.07608469727888689 1102 96-97 0.08371257682764721 1102 98-99 0.006780443563783933 1102 100-101 -0.0330597810834945 1102 102-103 0.007970543089729887 1102 104-105 0.1557105473248903 1102 106-107 0.16143390501206767 1102 108-109 0.09969909048338721 1102 110-111 0.04866451665922611 1102 112-113 0.19756140675839973 1102 114-115 0.22281390083064423 1102 116-117 0.22048631736889845 1102 118-119 0.29607784408220184 1102 120-121 0.19546617244528264 1102 122-123 0.26053251011080647 1102 124-125 0.21146499633888283 1103 1 0.12028590476491274 1103 2 0.19670351838710332 1103 3 0.16420720219941387 1103 4 0.1269099813874348 1103 5 0.11438414028719279 1103 6 0.15450751250668304 1103 7 0.14159491380168987 1103 8 0.1732358955522031 1103 9 0.19292771299744516 1103 10-11 0.1748892916355942 1103 12-13 0.2111229609466676 1103 14-15 0.28353333887456245 1103 16-17 0.19986321183929334 1103 18-19 0.16715712064652877 1103 20-21 0.13836256476776754 1103 22-23 0.20807442611219784 1103 24-25 0.19469679892476677 1103 26-27 0.22392128988977333 1103 28-29 0.21554893212998394 1103 30-31 0.262148251132551 1103 32-33 0.39280553539408913 1103 34-35 0.23360188883774669 1103 36-37 0.22684972170787887 1103 38-39 0.32120495814828587 1103 40-41 0.2544338332312037 1103 42-43 0.347924166450845 1103 44-45 0.3514719218876792 1103 46-47 0.24224743308602825 1103 48-49 0.297158490172059 1103 50-51 0.22071654577680277 1103 52-53 0.203324032716786 1103 54-55 0.17671430506000263 1103 56-57 0.19894942941304095 1103 58-59 0.22012991545233263 1103 60-61 0.1275640578225392 1103 62-63 0.11143085657196394 1103 64-65 0.22862652220234025 1103 66-67 0.10965800300229489 1103 68-69 0.0548478760359572 1103 70-71 0.07918071813433869 1103 72-73 0.13776741128707926 1103 74-75 0.17272630500864494 1103 76-77 0.158774274789657 1103 78-79 0.1067985954742312 1103 80-81 0.1493087829547335 1103 82-83 0.13792454170102886 1103 84-85 0.12781123226092106 1103 86-87 0.2051046073111209 1103 88-89 0.17015394696458586 1103 90-91 0.08145217511029657 1103 92-93 0.1817341396588148 1103 94-95 0.19887918820368355 1103 96-97 0.140294655850834 1103 98-99 0.22959372441717818 1103 100-101 0.16238943006915108 1103 102-103 0.19006670796640535 1103 104-105 0.14882651085264698 1103 106-107 0.06066615512250095 1103 108-109 0.20948106138207123 1103 110-111 0.2290346452878218 1103 112-113 0.17167976352969916 1103 114-115 0.34190051251204423 1103 116-117 0.3508313927593676 1103 118-119 0.3372380067963938 1103 120-121 0.3320481855388948 1103 122-123 0.25773445898901315 1103 124-125 0.3698486009724107 1104 1 0.11950939219742196 1104 2 0.1744144415916793 1104 3 0.1286658400938805 1104 4 0.07419133875360018 1104 5 0.1279863295369168 1104 6 0.14356811834969108 1104 7 0.1469795829172469 1104 8 0.15470371646667047 1104 9 0.26977862976534794 1104 10-11 0.16396589122210514 1104 12-13 0.19208910055964168 1104 14-15 0.2683856317572193 1104 16-17 0.2252097048683126 1104 18-19 0.30048700498891634 1104 20-21 0.2538342920724901 1104 22-23 0.21802694101411646 1104 24-25 0.17955676227624906 1104 26-27 0.19427427507304174 1104 28-29 0.2587165354642522 1104 30-31 0.23138608070974698 1104 32-33 0.38437405814013204 1104 34-35 0.3092870575456317 1104 36-37 0.3670453284550206 1104 38-39 0.2676908715770949 1104 40-41 0.2210566857447276 1104 42-43 0.2554232084582537 1104 44-45 0.3945530284458272 1104 46-47 0.36514547982562107 1104 48-49 0.38760963777211543 1104 50-51 0.4625736087118568 1104 52-53 0.5372035469508987 1104 54-55 0.5907745355396443 1104 56-57 0.6069003762752985 1104 58-59 0.3986158497709269 1104 60-61 0.46472261587884844 1104 62-63 0.5103327718390673 1104 64-65 0.4759807000619176 1104 66-67 0.4954584311123682 1104 68-69 0.5366483015348109 1104 70-71 0.5303100396275156 1104 72-73 0.5102959699113327 1104 74-75 0.47459254639405657 1104 76-77 0.46910822415411957 1104 78-79 0.42249975139756657 1104 80-81 0.49677938873898597 1104 82-83 0.46608825104835816 1104 84-85 0.5333179107912436 1104 86-87 0.49333634555686245 1104 88-89 0.49430828593663634 1104 90-91 0.5148292573161797 1104 92-93 0.41110155772678425 1104 94-95 0.38277835189409615 1104 96-97 0.46276948855301114 1104 98-99 0.4585040151834363 1104 100-101 0.4749549962574626 1104 102-103 0.43842849166701825 1104 104-105 0.508298952326971 1104 106-107 0.4151573615011941 1104 108-109 0.42078338295554474 1104 110-111 0.41978859688488157 1104 112-113 0.4689040656639172 1104 114-115 0.40711483846714813 1104 116-117 0.38810399599898204 1104 118-119 0.4143085927353489 1104 120-121 0.37032268256830037 1104 122-123 0.3438382459810754 1104 124-125 0.38658833837472883 1105 1 0.17832736859311638 1105 2 0.2527801295210139 1105 3 0.2096531577481784 1105 4 0.12179916395434986 1105 5 0.15882695957899529 1105 6 0.1379997728250757 1105 7 0.1934499392554585 1105 8 0.2062358424830606 1105 9 0.17538965530222583 1105 10-11 0.286949642901547 1105 12-13 0.2524683543508175 1105 14-15 0.32827742263578585 1105 16-17 0.32387997651130007 1105 18-19 0.25082645069389287 1105 20-21 0.3024614183448264 1105 22-23 0.36501088681449545 1105 24-25 0.27647246659284974 1105 26-27 0.34573147838878526 1105 28-29 0.36065744558559487 1105 30-31 0.3932455398170447 1105 32-33 0.43641575159260526 1105 34-35 0.2573904561909046 1105 36-37 0.29560500435285064 1105 38-39 0.3006684938217603 1105 40-41 0.3231729362591125 1105 42-43 0.2776518829114707 1105 44-45 0.38360464134476757 1105 46-47 0.36508664522927603 1105 48-49 0.3158297682453153 1105 50-51 0.12817787325613494 1105 52-53 0.17527504860258603 1105 54-55 0.11403414502417064 1105 56-57 0.12987379035052982 1105 58-59 0.0633261276098267 1105 60-61 0.12889208078983216 1105 62-63 0.19336670244881304 1105 64-65 0.11925457610506385 1105 66-67 0.06494142459004593 1105 68-69 0.12288801960740159 1105 70-71 0.19332209174499582 1105 72-73 0.021325296132211236 1105 74-75 0.03336382075141131 1105 76-77 0.0974091940274775 1105 78-79 0.09256829868827765 1105 80-81 0.09503166882889502 1105 82-83 0.05785808877788767 1105 84-85 -0.002450864591562407 1105 86-87 0.022358421494040215 1105 88-89 0.0489563187670079 1105 90-91 0.05981711478621321 1105 92-93 0.009466521026627817 1105 94-95 0.061686714184912006 1105 96-97 0.13921676976310593 1105 98-99 0.1304230268685842 1105 100-101 0.15787790565151738 1105 102-103 0.1675683032766493 1105 104-105 0.0969527170430986 1105 106-107 0.043964907220996 1105 108-109 0.10307257394327962 1105 110-111 0.14911704484152466 1105 112-113 0.04072614984459122 1105 114-115 -0.037157497239732606 1105 116-117 -0.06403372748107472 1105 118-119 0.018384999264458912 1105 120-121 0.037830262924060776 1105 122-123 -0.017872697320324704 1105 124-125 0.009821056792350902 1106 1 0.09521357677215292 1106 2 0.22570553499634372 1106 3 0.09173947588663367 1106 4 -0.03207059882439012 1106 5 0.0444471791744121 1106 6 0.023658154804664377 1106 7 0.09415950087316105 1106 8 0.07056889013752965 1106 9 0.0921099541330932 1106 10-11 0.05239441255275068 1106 12-13 0.13849930469120864 1106 14-15 0.2067297439113176 1106 16-17 0.2126618526129178 1106 18-19 0.12413331830599361 1106 20-21 0.12519601189926277 1106 22-23 0.1656028888843153 1106 24-25 0.1869071296804421 1106 26-27 0.09843373093968921 1106 28-29 0.09914437173491564 1106 30-31 0.16218572513098906 1106 32-33 0.14843108122828852 1106 34-35 0.12038992256434256 1106 36-37 0.09119352976479433 1106 38-39 0.0864122560506928 1106 40-41 -0.011485947006036668 1106 42-43 -0.04242945471244042 1106 44-45 -0.12546426116328036 1106 46-47 -0.1357716850047055 1106 48-49 -0.03133667472940971 1106 50-51 0.010429615627700173 1106 52-53 0.02439553501127989 1106 54-55 -0.00462920129945843 1106 56-57 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-0.8380685214121755 2216 52-53 -0.7765605202697472 2216 54-55 -0.664388469856398 2216 56-57 -0.6435538649937556 2216 58-59 -0.7428085367792931 2216 60-61 -0.7254525189133432 2216 62-63 -0.752885852786271 2216 64-65 -0.7307531246034031 2216 66-67 -0.7106564304126124 2216 68-69 -0.7953409574584853 2216 70-71 -0.7916370244937028 2216 72-73 -0.8657578155140513 2216 74-75 -0.8414261599268187 2216 76-77 -0.8721170023825877 2216 78-79 -0.8950405613268408 2216 80-81 -0.7524242971199726 2216 82-83 -0.7056741561886462 2216 84-85 -0.6291345194878097 2216 86-87 -0.7453952118402221 2216 88-89 -0.745928310618897 2216 90-91 -0.766615917052718 2216 92-93 -0.6820045943159876 2216 94-95 -0.6398408263488164 2216 96-97 -0.7827645525185467 2216 98-99 -0.7349580881939985 2216 100-101 -0.7979830335548854 2216 102-103 -0.8573679908624108 2216 104-105 -0.9341301425799848 2216 106-107 -0.8776033770611598 2216 108-109 -0.998874305169597 2216 110-111 -0.9614336258201774 2216 112-113 -1.0291822817311136 2216 114-115 -1.0320995319069226 2216 116-117 -0.9507073014498815 2216 118-119 -1.1194016771097672 2216 120-121 -1.0306850404838528 2216 122-123 -1.110295200190592 2216 124-125 -1.1431360152582783 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37.0 3 5.0 4 40.0 5 152.0 6 524.0 7 1001.0 8 1472.0 9 2386.0 10 4278.0 11 6082.0 12 7587.0 13 11312.0 14 18603.0 15 17914.0 16 6989.0 17 4993.0 18 4394.0 19 4029.0 20 3872.0 21 3824.0 22 3800.0 23 4115.0 24 4890.0 25 5852.0 26 7496.0 27 9428.0 28 11870.0 29 15067.0 30 18923.0 31 23769.0 32 30790.0 33 40289.0 34 55901.0 35 91604.0 36 173423.0 37 386715.0 38 206201.0 39 1265.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.68504037556161 18.874356897785812 13.56227380108273 21.878328925569846 2 16.348540679258466 21.270578118754347 39.52023298074953 22.86064822123766 3 18.789875773163846 27.25164289445001 31.084755383806677 22.87372594857946 4 12.197371261262093 17.372537919669682 37.4470544066104 32.98303641245783 5 11.95618584403719 39.36321008222712 34.07504568173268 14.60555839200301 6 29.60165729108148 37.65449981903819 17.504255331655553 15.239587558224777 7 26.32195026920997 35.186398094873425 21.35340568246323 17.138245953453378 8 26.809064130080646 34.3062175243431 19.76795544852094 19.116762897055317 9 28.004134715826456 14.564628866429533 20.339627774810815 37.091608642933195 10-11 24.773447130386298 27.106572216456236 27.162748595170676 20.957232057986786 12-13 26.363221853870876 25.448823235020473 28.010852369484386 20.17710254162426 14-15 23.091262683769813 25.50470571638738 27.97449306906084 23.42953853078197 16-17 20.699316142857622 28.996164219761322 28.342284606832525 21.96223503054853 18-19 20.447097756659883 25.73364833835133 33.17721635378193 20.64203755120686 20-21 21.80529384696513 24.640437587959276 33.59108130712105 19.963187257954544 22-23 21.368772315205746 25.061760428317598 33.32989053583365 20.23957672064301 24-25 20.694949667979966 25.15152507532169 33.75700735247193 20.39651790422641 26-27 20.632830025648914 25.313578895085477 33.58223712799309 20.471353951272516 28-29 20.4316176445891 25.251953997507748 33.82922212929468 20.48720622860847 30-31 20.647598878655195 25.02121309959807 33.941379210448645 20.389808811298092 32-33 20.48922152470585 25.141490580170156 33.55892054023371 20.810367354890282 34-35 20.843493784490953 25.110547388008314 33.5200841050238 20.525874722476935 36-37 21.138818633427718 24.905952848788974 33.096578027226656 20.858650490556656 38-39 20.855795487752037 25.175792599160967 33.127605190059214 20.84080672302778 40-41 21.01567564481078 25.14787235114519 33.070295207289995 20.76615679675403 42-43 21.100522986903496 25.072469122947265 32.649313264788574 21.177694625360665 44-45 21.06101980700181 25.343104160578793 32.31451718543747 21.281358846981927 46-47 21.447074965655997 25.598542940921593 31.44294360865637 21.51143848476604 48-49 21.639639405121738 25.946255055671696 30.645904176416227 21.76820136279034 50-51 21.54620234244583 26.244949164155944 30.183509503800515 22.025338989597714 52-53 21.80192474030282 26.373056994818654 30.012764420856392 21.812253844022138 54-55 21.51143848476604 26.281434420585576 30.086145511095886 22.120981583552496 56-57 21.660780322648908 26.49597948428573 29.696311672259114 22.146928520806252 58-59 21.795807319766393 26.106173140143675 29.86677974783459 22.231239792255337 60-61 21.67975769423256 26.19683396983102 30.17939494093503 21.944013395001395 62-63 21.811148819003098 26.030257469374522 30.301176567402493 21.85741714421988 64-65 21.45551401806377 25.97968581533842 30.406241707896264 22.158558458701545 66-67 21.707631763796513 26.32614014190448 29.877530867100273 22.088697227198736 68-69 21.730407892057347 26.052564729691994 30.09961643996666 22.117410938283992 70-71 21.485890065667345 26.145840375318752 30.240066444150425 22.128203114863474 72-73 21.759823728768016 26.31968306110042 29.781541903044108 22.13895130708746 74-75 21.422261632457015 26.538258716995326 29.63547492131948 22.404004729228173 76-77 21.455892046671174 26.466157521507117 29.707570420317666 22.37038001150405 78-79 21.501470333852275 26.57416410974686 29.488338934205288 22.436026622195577 80-81 21.259880207340554 26.694256653211756 29.485234165007547 22.560628974440146 82-83 21.413318576713987 26.641561940014007 29.281924764753402 22.663194718518607 84-85 21.401856445412545 26.4970047309632 29.076530089564166 23.02460873406008 86-87 21.286607013902184 26.309018785918454 29.166792622672755 23.237581577506607 88-89 21.347023911488193 26.29155288640784 29.2120108288577 23.14941237324627 90-91 21.235815135336665 26.461857681953376 29.261505338060363 23.040821844649596 92-93 21.179901690954217 26.349251135651286 29.220468133114828 23.25037904027967 94-95 21.055670206981397 26.17673301520187 29.214713545220366 23.55288323259637 96-97 21.417283295434316 26.027807914120938 29.14288881200856 23.412019978436188 98-99 21.113783985901556 26.017377838448162 29.002800290064535 23.86603788558575 100-101 20.945863380421862 26.241611555550705 29.155190578991107 23.65733448503633 102-103 21.337020681487452 26.145524260692994 28.890918750249355 23.62653630757019 104-105 21.08927657426796 26.063035800737232 28.95945119224521 23.888236432749594 106-107 20.8952835581188 26.25892950515558 29.031575242617524 23.814211694108092 108-109 21.14943001804187 26.363345439640373 28.48112323235038 24.006101309967377 110-111 20.947943330022614 26.348935705571456 28.53867135906734 24.164449605338586 112-113 20.89557816259856 26.54215111579879 28.400227236751558 24.162043484851093 114-115 20.76926855627057 26.644607432542788 28.3314208892634 24.254703121923242 116-117 20.59893172064184 26.661723572429626 28.423267698782507 24.316077008146028 118-119 20.6230745684955 26.703417731458018 28.172508585582268 24.500999114464214 120-121 20.480194677603006 26.833037756572388 28.213263671264897 24.47350389455971 122-123 20.485411498073073 27.011600181881313 27.894555862376475 24.608432457669142 124-125 20.340303700974964 27.04363470025795 27.79194496401684 24.824116634750244 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 513.0 1 478.0 2 837.5 3 1203.5 4 757.5 5 517.0 6 1330.0 7 3193.5 8 5288.0 9 5972.5 10 4335.0 11 2467.5 12 2014.0 13 2049.5 14 2193.0 15 2346.0 16 2573.0 17 2756.5 18 2889.5 19 3068.5 20 3337.5 21 3729.0 22 4175.5 23 4805.0 24 5587.5 25 6693.5 26 8404.5 27 10022.5 28 11429.0 29 13374.0 30 15690.0 31 17858.0 32 20589.5 33 23739.0 34 27049.5 35 29022.5 36 31262.0 37 34537.5 38 34920.0 39 34217.0 40 34636.0 41 35929.5 42 36773.0 43 37187.5 44 38058.0 45 39098.0 46 39268.0 47 39209.5 48 40280.0 49 40721.0 50 40123.5 51 38375.5 52 36408.5 53 35355.0 54 34258.0 55 32703.5 56 30399.5 57 28131.0 58 26900.0 59 24507.0 60 21211.5 61 17739.5 62 14341.5 63 11103.0 64 8199.0 65 6256.0 66 4367.5 67 3340.0 68 2459.0 69 1467.0 70 1056.5 71 869.5 72 774.0 73 749.5 74 595.5 75 444.0 76 298.5 77 148.5 78 88.0 79 38.5 80 16.5 81 13.0 82 9.0 83 5.0 84 2.5 85 2.0 86 2.0 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047695340971305546 2 0.005374122926344286 3 5.038240243447769E-4 4 0.02376369981492864 5 0.0036947095118616967 6 0.0027710321338962726 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 9.65662713327489E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.9389734753445314E-4 28-29 0.0 30-31 4.1985335362064737E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.003358826828965179 44-45 0.0 46-47 0.0 48-49 0.002225222774189431 50-51 0.0 52-53 0.006885594999378617 54-55 0.0 56-57 0.0 58-59 5.877946950689063E-4 60-61 0.0 62-63 0.0010496333840516183 64-65 0.0 66-67 0.0017213987498446543 68-69 0.006255814968947646 70-71 0.009866553810085213 72-73 0.0 74-75 0.0 76-77 5.877946950689063E-4 78-79 5.038240243447769E-4 80-81 8.397067072412947E-5 82-83 0.0011755893901378126 84-85 0.0011755893901378126 86-87 0.0 88-89 0.0 90-91 8.397067072412948E-4 92-93 0.004660372225189186 94-95 0.00709552167618894 96-97 6.717653657930358E-4 98-99 0.01028640716370586 100-101 0.016080383443670795 102-103 0.02808818935722131 104-105 0.007767287041981977 106-107 0.03245466423487604 108-109 0.02754237999751447 110-111 0.026408775942738723 112-113 0.005332137590982222 114-115 6.29780030430971E-4 116-117 4.198533536206474E-4 118-119 0.0073474336883613284 120-121 0.0 122-123 4.618386889827121E-4 124-125 0.04563805953856437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1190892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.12829400286363 #Duplication Level Percentage of deduplicated Percentage of total 1 81.34309462624441 47.28335319537092 2 10.903143910506266 12.67562309570881 3 3.374347364150071 5.884351669533099 4 1.4644725256686943 3.405091581247445 5 0.7918116167468908 2.3013329226573025 6 0.4480717442944297 1.562738765203353 7 0.29764711293927093 1.2111203223026248 8 0.21576786990332833 1.003377454248984 9 0.14823676789461443 0.7755075383591163 >10 0.8886680338524087 9.69076570926813 >50 0.07764335201011939 3.1043927489732464 >100 0.042767420176462116 4.492552035423795 >500 0.0025456797723864633 1.0146715845437246 >1k 0.0010182719089545853 1.1866197223940687 >5k 2.545679772386463E-4 1.0134877763657533 >10k+ 5.091359544772926E-4 3.39501387839969 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12143 1.0196558546031043 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10800 0.9068832438205985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6849 0.5751151237895628 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2823 0.2370492034542175 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2292 0.19246077729970476 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1542 0.12948277425660765 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1362 0.11436805352626435 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 8.397067072412947E-5 0.0 0.0 8.397067072412947E-5 0.0 20-21 1.6794134144825895E-4 0.0 0.0 2.9389734753445314E-4 0.0 22-23 2.099266768103237E-4 0.0 0.0 5.458093597068415E-4 0.0 24-25 5.038240243447769E-4 0.0 0.0 0.001133604054775748 0.0 26-27 5.038240243447769E-4 0.0 0.0 0.002729046798534208 0.0 28-29 7.137507011551005E-4 0.0 0.0 0.005961917621413192 0.0 30-31 7.557360365171653E-4 0.0 0.0 0.017130016827722413 0.0 32-33 7.557360365171653E-4 0.0 0.0 0.03740893380759968 0.0 34-35 8.397067072412948E-4 0.0 0.0 0.07078727542044114 0.0 36-37 0.0010916187194136833 0.0 0.0 0.12007805913550515 0.0 38-39 0.0010916187194136833 0.0 0.0 0.19430813205563563 0.0 40-41 0.001133604054775748 0.0 0.0 0.2802101282064201 0.0 42-43 0.0013015453962240068 0.0 0.0 0.35985630938825686 0.0 44-45 0.001427501402310201 0.0 0.0 0.45285382721523026 0.0 46-47 0.0016374280791205248 0.0 0.0 0.5671798954061326 0.0 48-49 0.001763384085206719 0.0 0.0 0.6954870802726024 0.0 50-51 0.001763384085206719 0.0 0.0 0.8378593524853639 0.0 52-53 0.001763384085206719 0.0 0.0 0.9708688949123849 0.0 54-55 0.001763384085206719 0.0 0.0 1.1163900672773015 0.0 56-57 0.0019313254266549779 0.0 0.0 1.284835232749905 0.0 58-59 0.0020152960973791076 0.0 0.0 1.4801510128542303 0.0 60-61 0.0020152960973791076 0.0 0.0 1.7088451345713969 0.0 62-63 0.0020152960973791076 0.0 0.0 1.9368674909227703 0.0 64-65 0.0021832374388273666 0.0 0.0 2.1598096216953344 0.0 66-67 0.002519120121723884 0.0 0.0 2.414534651336981 0.0 68-69 0.002519120121723884 0.0 0.0 2.7171229632913816 0.0 70-71 0.002561105457085949 0.0 0.0 3.061066830577416 0.0 72-73 0.0026030907924480136 0.0 0.0 3.410174894112984 0.0 74-75 0.0026030907924480136 0.0 0.0 3.753698908045398 0.0 76-77 0.0026030907924480136 0.0 0.0 4.116998014933344 0.0 78-79 0.0026030907924480136 0.0 0.0 4.5445346849252495 0.0 80-81 0.0034427974996893086 0.0 0.0 5.028583616314494 0.0 82-83 0.003526768170413438 0.0 0.0 5.546556698676286 0.0 84-85 0.003652724176499632 0.0 0.0 6.038456887778237 0.0 86-87 0.0037786801825858266 0.0 0.0 6.529475384837584 0.0 88-89 0.0037786801825858266 0.0 0.0 7.0738152578067535 0.0 90-91 0.003946621524034085 0.0 0.0 7.6873469634526055 0.0 92-93 0.003946621524034085 0.0 0.0 8.35503975171552 0.0 94-95 0.003946621524034085 0.0 0.0 9.004846787114197 0.0 96-97 0.003946621524034085 0.0 0.0 9.64974153827551 0.0 98-99 0.00407257753012028 0.0 0.0 10.328854337756908 0.0 100-101 0.004282504206930604 0.0 0.0 11.093365309364746 0.0 102-103 0.004450445548378862 0.0 0.0 11.904102135206216 0.0 104-105 0.005206181584896027 0.0 0.0 12.702369316445152 0.0 106-107 0.005458093597068416 0.0 0.0 13.486949278356056 0.0 108-109 0.005542064267792545 0.0 0.0 14.262460407828755 0.0 110-111 0.005710005609240804 0.0 0.0 15.10850690070972 0.0 112-113 0.005793976279964934 0.0 0.0 16.041547008460885 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4870 0.0 80.564354 1 GTATCAA 8965 0.0 54.1909 1 CGTATAG 85 3.4661716E-6 42.026073 1 TGGTATC 1645 0.0 40.883972 2 ATCAACG 11880 0.0 40.474426 3 TCAACGC 11980 0.0 40.235924 4 CAACGCA 12085 0.0 39.776157 5 TATCAAC 12235 0.0 39.256363 2 AACGCAG 12430 0.0 39.007183 6 GTGGTAT 1850 0.0 37.009445 1 ACGCAGA 14220 0.0 34.011894 7 CGCAGAG 14345 0.0 33.63258 8 GTACATG 9225 0.0 33.431038 1 TACATGG 9420 0.0 32.60163 2 ACATGGG 9350 0.0 31.56864 3 GCAGAGT 15240 0.0 31.540333 9 GAGTACT 10455 0.0 30.357677 12-13 ATGGGAT 2600 0.0 29.059525 5 CATGGGA 7050 0.0 28.868402 4 GTACTTT 11120 0.0 28.48872 14-15 >>END_MODULE