FastQCFastQC Report
Thu 26 May 2016
SRR1364214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805464
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.043.4371031
GTATCAA38350.039.0976451
ATCAACG40400.035.3413663
GTACATG95800.034.966991
TCAACGC41650.034.281314
CAACGCA41500.034.264925
AACGCAG42150.033.7178766
TACATGG98950.032.707852
ACATGGG99150.031.620693
TATCAAC49800.029.3883132
CATGGGG64400.029.0071434
ACGCAGA54600.025.7031887
CGCAGAG55350.025.4623438
ATGGGGA41150.021.5435035
GAGTACA70850.020.0712051
GCAGAGT71050.019.2503729
GTACCGT2801.5767255E-619.1136826
GTATAGG11650.018.8969291
CATGGGA50050.018.661944
AGAGTAC61050.018.11768210-11
Produced by FastQC (version 0.11.5)