##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2805464 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16442235580282 33.0 31.0 34.0 28.0 34.0 2 31.16393224079867 33.0 31.0 34.0 28.0 34.0 3 31.258263160746314 33.0 31.0 34.0 28.0 34.0 4 34.67225421534548 37.0 35.0 37.0 32.0 37.0 5 34.741839139621824 37.0 35.0 37.0 32.0 37.0 6 34.85262509160695 37.0 35.0 37.0 32.0 37.0 7 34.722819112988084 37.0 35.0 37.0 32.0 37.0 8 34.70426603228557 37.0 35.0 37.0 32.0 37.0 9 36.25556734999986 39.0 37.0 39.0 32.0 39.0 10-11 36.19754183265228 39.0 37.0 39.0 32.0 39.0 12-13 36.164331283523865 39.0 37.0 39.0 32.0 39.0 14-15 37.485017808105894 40.0 38.0 41.0 32.0 41.0 16-17 37.51357761140403 40.0 38.0 41.0 32.0 41.0 18-19 37.39891885976794 40.0 38.0 41.0 31.0 41.0 20-21 37.381845391707046 40.0 38.0 41.0 31.0 41.0 22-23 37.40829181910728 40.0 38.0 41.0 31.0 41.0 24-25 37.254698509765234 40.0 38.0 41.0 30.0 41.0 26-27 37.27147969106002 40.0 37.5 41.0 30.0 41.0 28-29 37.35743035733127 40.0 38.0 41.0 31.0 41.0 30-31 37.255780327247116 40.0 37.0 41.0 30.0 41.0 32-33 37.079711413156616 40.0 37.0 41.0 30.0 41.0 34-35 37.03742981553141 40.0 37.0 41.0 30.0 41.0 36-37 37.05958319194258 40.0 37.0 41.0 30.0 41.0 38-39 36.8966989061346 40.0 37.0 41.0 30.0 41.0 40-41 36.90261931716108 40.0 37.0 41.0 30.0 41.0 42-43 36.827876600804714 40.0 37.0 41.0 30.0 41.0 44-45 36.81074307137786 40.0 37.0 41.0 30.0 41.0 46-47 36.73267149391331 40.0 36.0 41.0 30.0 41.0 48-49 36.64365502462338 40.0 36.0 41.0 30.0 41.0 50-51 36.52384097603819 40.0 36.0 41.0 30.0 41.0 52-53 36.37467955389911 40.0 35.5 41.0 30.0 41.0 54-55 36.187678401861504 39.0 35.0 41.0 29.0 41.0 56-57 35.9989999871679 39.0 35.0 41.0 29.0 41.0 58-59 35.768829683788496 39.0 35.0 41.0 29.0 41.0 60-61 35.518503890978465 38.5 35.0 40.0 28.5 41.0 62-63 35.21986398684852 38.0 34.0 40.0 28.0 41.0 64-65 34.90642635228968 37.0 34.0 40.0 28.0 41.0 66-67 34.59854893878517 37.0 34.0 40.0 28.0 41.0 68-69 34.3648460646795 36.0 34.0 39.0 28.0 41.0 70-71 34.20084324732023 36.0 34.0 39.0 28.0 41.0 72-73 33.89150939024704 35.5 34.0 39.0 28.0 40.5 74-75 33.56149285822238 35.0 34.0 37.5 27.5 40.0 76-77 33.23298855376508 35.0 34.0 37.0 27.0 39.0 78-79 32.89979108625169 35.0 33.0 36.5 26.5 39.0 80-81 32.611236501341665 35.0 33.0 36.0 26.0 38.5 82-83 32.35214584824472 35.0 33.0 36.0 26.0 37.0 84-85 32.117636333954025 35.0 33.0 35.5 26.0 37.0 86-87 31.910567556739277 35.0 33.0 35.0 26.0 36.5 88-89 31.746508777157718 35.0 33.0 35.0 25.0 36.0 90-91 31.605433361468904 35.0 33.0 35.0 25.0 36.0 92-93 31.478697641459668 35.0 33.0 35.0 25.0 36.0 94-95 31.35682404051522 35.0 33.0 35.0 24.5 35.5 96-97 31.26895871770231 35.0 33.0 35.0 24.0 35.0 98-99 31.195995742593738 35.0 33.0 35.0 24.0 35.0 100-101 31.094632296119286 35.0 32.0 35.0 23.5 35.0 102-103 30.085135114904347 34.0 30.5 35.0 20.0 35.0 104-105 30.795669985428436 34.5 32.0 35.0 23.0 35.0 106-107 30.903013547848055 35.0 32.0 35.0 23.0 35.0 108-109 30.86971941183348 35.0 32.0 35.0 21.5 35.0 110-111 30.804791292991105 35.0 32.0 35.0 20.0 35.0 112-113 30.95690106877151 35.0 33.0 35.0 23.0 35.0 114-115 30.949034277395825 35.0 33.0 35.0 21.5 35.0 116-117 30.85715749694168 35.0 33.0 35.0 19.5 35.0 118-119 30.615708488863163 35.0 33.0 35.0 16.0 35.0 120-121 30.554407042827854 35.0 33.0 35.0 11.5 35.0 122-123 30.423145868205758 35.0 33.0 35.0 2.0 35.0 124-125 29.55136316131663 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36632127485418664 1101 2 0.45940930203731867 1101 3 0.45885590446745894 1101 4 0.4440196923244599 1101 5 0.39643565500432487 1101 6 0.4275313861988934 1101 7 0.4261413932833591 1101 8 0.3989161565509889 1101 9 0.4217294332791255 1101 10-11 0.47846931710808605 1101 12-13 0.445603772689374 1101 14-15 0.5329108277706638 1101 16-17 0.5171634737200748 1101 18-19 0.6459022198021671 1101 20-21 0.7141277334297698 1101 22-23 0.6378570572601419 1101 24-25 0.9330256461194182 1101 26-27 0.7985555634481756 1101 28-29 0.7425966859479587 1101 30-31 0.7580112284326859 1101 32-33 0.9066621254239067 1101 34-35 0.836570695220523 1101 36-37 0.8088582321638924 1101 38-39 0.7896349738168951 1101 40-41 0.6995662859661209 1101 42-43 0.7193875253763267 1101 44-45 0.5301749210222653 1101 46-47 0.5537594485040742 1101 48-49 0.5748701894429686 1101 50-51 0.46951750305215967 1101 52-53 0.481941340584747 1101 54-55 0.5184026596440674 1101 56-57 0.5198159853765816 1101 58-59 0.5803439318896864 1101 60-61 0.423147025946335 1101 62-63 0.39821064979531684 1101 64-65 0.3801741422881477 1101 66-67 0.33671924910330375 1101 68-69 0.22969341824806122 1101 70-71 0.28757297771855406 1101 72-73 0.23616784496245202 1101 74-75 0.3242708857077261 1101 76-77 0.36463834303494025 1101 78-79 0.298565121256118 1101 80-81 0.2472845698598647 1101 82-83 0.31067533530944047 1101 84-85 0.2658000511493128 1101 86-87 0.26930813277245846 1101 88-89 0.32789816190721766 1101 90-91 0.24876189164055873 1101 92-93 0.3033769415665972 1101 94-95 0.3665622911058719 1101 96-97 0.37301146726417755 1101 98-99 0.3293702488446293 1101 100-101 0.3009134388185437 1101 102-103 0.136039300827143 1101 104-105 0.26453489369957595 1101 106-107 0.30530942153192697 1101 108-109 0.33803246871294945 1101 110-111 0.22562476825482491 1101 112-113 0.2597465193092141 1101 114-115 0.3063450744997027 1101 116-117 0.33689482636100365 1101 118-119 0.1980918264294047 1101 120-121 0.18388576944454726 1101 122-123 0.19782731724895086 1101 124-125 0.07885137743408777 1102 1 0.011726593385791517 1102 2 0.0216419708341391 1102 3 0.03360378040746781 1102 4 0.0651096534033897 1102 5 0.00298900334225749 1102 6 0.06086445606250379 1102 7 0.06096853409014358 1102 8 0.12155683456397526 1102 9 0.1335612658230403 1102 10-11 0.10841143280949694 1102 12-13 0.1850072179512381 1102 14-15 0.1932037995181446 1102 16-17 0.19849677543704303 1102 18-19 0.24373264541775086 1102 20-21 0.3065756042979899 1102 22-23 0.3295892455540539 1102 24-25 0.42817450980741967 1102 26-27 0.4506288725818166 1102 28-29 0.2923441502165218 1102 30-31 0.3961762013183616 1102 32-33 0.4836790685032497 1102 34-35 0.4081416532161981 1102 36-37 0.29047318301690694 1102 38-39 0.3562852065594626 1102 40-41 0.24505891177786054 1102 42-43 0.26013802547479514 1102 44-45 0.11261766081759106 1102 46-47 0.0964748728780549 1102 48-49 0.050949871909253375 1102 50-51 -0.007315636960264271 1102 52-53 -0.04404217543189759 1102 54-55 0.02781402875790917 1102 56-57 0.03676390597470913 1102 58-59 0.02603778270390933 1102 60-61 0.03391789884128826 1102 62-63 0.07033614569473912 1102 64-65 -0.007241489765839049 1102 66-67 -0.07520881575766936 1102 68-69 0.03996445614093602 1102 70-71 0.06973752641796693 1102 72-73 -0.023127893875823702 1102 74-75 -0.02095382432737125 1102 76-77 -0.004960675803808101 1102 78-79 0.05030043166626541 1102 80-81 0.056968136133370706 1102 82-83 0.0041555464277251986 1102 84-85 -0.00793722079293957 1102 86-87 0.014696885645101077 1102 88-89 -0.036704054795315244 1102 90-91 -0.12314782255407053 1102 92-93 -0.03708173287769512 1102 94-95 -0.045523690921541515 1102 96-97 -0.03894034280576619 1102 98-99 -0.020176912565485594 1102 100-101 -0.14676987188932955 1102 102-103 -0.22786752046990344 1102 104-105 -0.08381094456558102 1102 106-107 -0.1258230503871438 1102 108-109 -0.14340572688829312 1102 110-111 -0.12284341876389249 1102 112-113 -0.04540428088127513 1102 114-115 -0.027713096980882312 1102 116-117 -0.04609520592062211 1102 118-119 -0.2082444887225421 1102 120-121 -0.22295189566040108 1102 122-123 -0.1793629559757406 1102 124-125 -0.2257235767386483 1103 1 0.18190036828919176 1103 2 -0.06540565326989167 1103 3 0.28544916926211883 1103 4 0.2891825932546226 1103 5 0.2862086733320979 1103 6 0.3054511693650923 1103 7 0.3687757553452258 1103 8 0.37377342168282723 1103 9 0.4578240905695452 1103 10-11 0.43726165530137706 1103 12-13 0.3745338042353197 1103 14-15 0.5070112171507333 1103 16-17 0.4545021371186735 1103 18-19 0.5453658090369942 1103 20-21 0.6127944065806972 1103 22-23 0.5975006172509367 1103 24-25 0.7792766066010657 1103 26-27 0.7840630706051783 1103 28-29 0.7199259147419781 1103 30-31 0.718894903973073 1103 32-33 0.9025112236248418 1103 34-35 0.8223351413508766 1103 36-37 0.6795179087936347 1103 38-39 0.8676512104147278 1103 40-41 0.7740307667872912 1103 42-43 0.7108407786938358 1103 44-45 0.6282982128238856 1103 46-47 0.6760782862645343 1103 48-49 0.5561275855569505 1103 50-51 0.5966812209996277 1103 52-53 0.5426272460636312 1103 54-55 0.5039422639094155 1103 56-57 0.5256419347539207 1103 58-59 0.5325848936357716 1103 60-61 0.48275605722966475 1103 62-63 0.42301620291927833 1103 64-65 0.49188018464985106 1103 66-67 0.4720863719379693 1103 68-69 0.4328052166911931 1103 70-71 0.3969459817809451 1103 72-73 0.47174003265595843 1103 74-75 0.47102923987176126 1103 76-77 0.43859137750853705 1103 78-79 0.3751666534877742 1103 80-81 0.379391728811882 1103 82-83 0.3934912243507114 1103 84-85 0.34753485122323013 1103 86-87 0.31903190311278706 1103 88-89 0.3211465837993934 1103 90-91 0.3071520433399222 1103 92-93 0.35361377932058247 1103 94-95 0.4051360447492165 1103 96-97 0.43899712407152336 1103 98-99 0.3473506274608269 1103 100-101 0.3050204097268896 1103 102-103 0.253914148041666 1103 104-105 0.3248294738272506 1103 106-107 0.354094918826366 1103 108-109 0.307728097346164 1103 110-111 0.31801045878034273 1103 112-113 0.3879311846725422 1103 114-115 0.3780352628351622 1103 116-117 0.46569841475287177 1103 118-119 0.3514352752150671 1103 120-121 0.3568656779515784 1103 122-123 0.3267920396089643 1103 124-125 0.24689205970837946 1104 1 0.2973898263057748 1104 2 0.3380401729903504 1104 3 0.35600972723687363 1104 4 0.2843107699958338 1104 5 0.31146453477328606 1104 6 0.3171992688558305 1104 7 0.3447764442029495 1104 8 0.2893345332039772 1104 9 0.3958573402558727 1104 10-11 0.4298021564453691 1104 12-13 0.4694903815965006 1104 14-15 0.47786161816434003 1104 16-17 0.46081087454086855 1104 18-19 0.5719595613434336 1104 20-21 0.5975712658815127 1104 22-23 0.6364615184244542 1104 24-25 0.78041506700405 1104 26-27 0.7883934947447244 1104 28-29 0.6067867224276711 1104 30-31 0.649966692788972 1104 32-33 0.809020006560857 1104 34-35 0.8588575169259443 1104 36-37 0.700725347226971 1104 38-39 0.8243970957199238 1104 40-41 0.7455554180530584 1104 42-43 0.5844933545296911 1104 44-45 0.605648017237499 1104 46-47 0.5689289271482423 1104 48-49 0.45920576475836583 1104 50-51 0.4326397480742017 1104 52-53 0.5184609484278937 1104 54-55 0.46230465842807433 1104 56-57 0.35131203340819184 1104 58-59 0.397144547484757 1104 60-61 0.4590833384558266 1104 62-63 0.4192273316618653 1104 64-65 0.5000935829326281 1104 66-67 0.4934423453185346 1104 68-69 0.44917681238091234 1104 70-71 0.49608490962310015 1104 72-73 0.4708302176634689 1104 74-75 0.4618981846893391 1104 76-77 0.4489416186087354 1104 78-79 0.48548752587687716 1104 80-81 0.4027489261450228 1104 82-83 0.43394254917173214 1104 84-85 0.46587415055651604 1104 86-87 0.4398992180437773 1104 88-89 0.4462377651903928 1104 90-91 0.46438166668235326 1104 92-93 0.48746836883518085 1104 94-95 0.48702892737278347 1104 96-97 0.4677314095046441 1104 98-99 0.438309677329201 1104 100-101 0.43259378537576865 1104 102-103 0.39107141057026595 1104 104-105 0.3926945684221792 1104 106-107 0.4006763085968288 1104 108-109 0.3521731055878554 1104 110-111 0.4139569615972789 1104 112-113 0.4967591732079839 1104 114-115 0.44812669024678087 1104 116-117 0.47023195966393416 1104 118-119 0.42122794134048647 1104 120-121 0.35946811478578766 1104 122-123 0.3773331312024375 1104 124-125 0.4266131953395451 1105 1 0.4140326970463022 1105 2 0.4076218406088543 1105 3 0.3604357694984408 1105 4 0.4783665476467007 1105 5 0.38882984983135316 1105 6 0.3717538091185375 1105 7 0.43914156889360356 1105 8 0.3829738821474322 1105 9 0.49160976744033746 1105 10-11 0.47524404679374044 1105 12-13 0.5176152626168715 1105 14-15 0.5100372796910904 1105 16-17 0.5639102939202658 1105 18-19 0.6621972826455504 1105 20-21 0.7827678202314061 1105 22-23 0.6346803933530722 1105 24-25 0.8712274599223804 1105 26-27 0.8648057829609783 1105 28-29 0.8198375981177435 1105 30-31 0.7635470278356138 1105 32-33 0.9319568997647423 1105 34-35 0.9868510444340401 1105 36-37 0.8547313913922139 1105 38-39 0.9345984092550381 1105 40-41 0.8165148697949292 1105 42-43 0.801580019292679 1105 44-45 0.7114018721775324 1105 46-47 0.6235096429297045 1105 48-49 0.6417764386589937 1105 50-51 0.5442628681960358 1105 52-53 0.5666096253743973 1105 54-55 0.4843932268651585 1105 56-57 0.6078892273925831 1105 58-59 0.6601192762112049 1105 60-61 0.562479017115642 1105 62-63 0.6200923135769614 1105 64-65 0.5882622484570916 1105 66-67 0.6364332552747456 1105 68-69 0.7246229859959925 1105 70-71 0.7435625414525333 1105 72-73 0.655002357393144 1105 74-75 0.6301710763702815 1105 76-77 0.6698213539980387 1105 78-79 0.6780275030327942 1105 80-81 0.5457053320083389 1105 82-83 0.5360770437462605 1105 84-85 0.6037977180417613 1105 86-87 0.5844987326068818 1105 88-89 0.6155326918989381 1105 90-91 0.5606576042418503 1105 92-93 0.5221104781060717 1105 94-95 0.596747183607313 1105 96-97 0.5500391384214538 1105 98-99 0.5274611146031418 1105 100-101 0.5073962791888285 1105 102-103 0.4979067903532446 1105 104-105 0.5213805573808727 1105 106-107 0.5710967826116793 1105 108-109 0.44501172161679037 1105 110-111 0.511724903725586 1105 112-113 0.5236426207617626 1105 114-115 0.4945180380480352 1105 116-117 0.4915340727421764 1105 118-119 0.4120236771105219 1105 120-121 0.41299348124304913 1105 122-123 0.4877093447946734 1105 124-125 0.37064755665688764 1106 1 0.2442668982506575 1106 2 0.29507371589438947 1106 3 0.250015750076372 1106 4 0.3133067547695063 1106 5 0.2108734943845647 1106 6 0.27062762766684045 1106 7 0.1681440414191826 1106 8 0.30736080140810884 1106 9 0.3297158174372399 1106 10-11 0.3414767109917136 1106 12-13 0.31417530687897965 1106 14-15 0.43011980625868773 1106 16-17 0.44331366224852786 1106 18-19 0.5418527533092785 1106 20-21 0.513549988891171 1106 22-23 0.5346472771214081 1106 24-25 0.7187007832768444 1106 26-27 0.6973050311019975 1106 28-29 0.5849149535025475 1106 30-31 0.6371102023564319 1106 32-33 0.7878122742364297 1106 34-35 0.773066943898435 1106 36-37 0.6490153117112314 1106 38-39 0.6624195735689469 1106 40-41 0.6185460082356968 1106 42-43 0.5204277466123628 1106 44-45 0.4420672444223399 1106 46-47 0.4927791860978843 1106 48-49 0.4414591656457958 1106 50-51 0.47512438380599775 1106 52-53 0.4877031440055788 1106 54-55 0.47289954133277234 1106 56-57 0.4490544538571015 1106 58-59 0.41068454790205067 1106 60-61 0.3615013995558911 1106 62-63 0.352636501075672 1106 64-65 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2216 74-75 -2.359432288428181 2216 76-77 -2.361212664623544 2216 78-79 -2.3523392715087397 2216 80-81 -2.361137894405985 2216 82-83 -2.33951624362809 2216 84-85 -2.347608605415072 2216 86-87 -2.3037327389584377 2216 88-89 -2.239116851339766 2216 90-91 -2.3266510544229604 2216 92-93 -2.317136489985984 2216 94-95 -2.2357097883666768 2216 96-97 -2.365367167478894 2216 98-99 -2.3584021086510134 2216 100-101 -2.366072136689258 2216 102-103 -2.334498194909056 2216 104-105 -2.3645332383123296 2216 106-107 -2.399473276553689 2216 108-109 -2.3557704199236156 2216 110-111 -2.364525971660413 2216 112-113 -2.378876028129934 2216 114-115 -2.4218415607667083 2216 116-117 -2.3594448477678966 2216 118-119 -2.2214039004257558 2216 120-121 -2.096421551207193 2216 122-123 -2.111155164959907 2216 124-125 -2.079280681198412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41349.0 3 8105.0 4 5315.0 5 4518.0 6 4975.0 7 5682.0 8 5949.0 9 6514.0 10 7240.0 11 8278.0 12 9116.0 13 9019.0 14 9226.0 15 8891.0 16 8455.0 17 7683.0 18 7133.0 19 6867.0 20 6838.0 21 7325.0 22 8470.0 23 11230.0 24 16686.0 25 24334.0 26 29912.0 27 29921.0 28 35120.0 29 42081.0 30 50632.0 31 61400.0 32 79011.0 33 106549.0 34 152801.0 35 239257.0 36 485655.0 37 886087.0 38 366527.0 39 1313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54047013034447 18.101630447188803 12.313564327618874 25.044335094847852 2 15.852268553091726 19.830251701655545 39.476803776273755 24.840675968978978 3 18.960603335204375 24.209973375138098 29.031476963039726 27.7979463266178 4 12.706064603372777 15.979918385454168 36.674765357289196 34.63925165388385 5 13.895227610938335 37.85203086948723 34.29410885769988 13.958632661874557 6 33.280089486734546 36.06346771077363 17.056769038388325 13.599673764103501 7 28.7662331412904 32.042357569144905 21.111273097461407 18.080136192103296 8 25.90316610728208 36.03457395995814 19.711391769774984 18.350868162984803 9 26.29330871449755 15.974642123873616 19.341707539637277 38.39034162199156 10-11 25.078046801703017 26.373937394309767 27.809631392387736 20.738384411599476 12-13 25.419953707111485 23.791771472038644 27.724492616646085 23.063782204203786 14-15 22.52937937785392 25.469179747982484 24.881886283230873 27.119554590932726 16-17 23.191465130193336 28.357885217557445 25.602595436527352 22.84805421572187 18-19 23.183253598238494 27.476686351806613 26.753028430513194 22.5870316194417 20-21 23.733893385673962 26.843620486707962 26.643849631436318 22.778636496181754 22-23 23.62117099886025 26.68720612818435 26.458329694007894 23.233293178947502 24-25 23.334082492193094 26.864706009210554 26.60750272201317 23.193708776583183 26-27 23.268152436275585 27.131055974026275 26.35681037551491 23.243981214183233 28-29 23.179425281292197 27.204423962345132 26.428811792670075 23.187338963692593 30-31 23.26749543894821 26.933893088518268 26.487911697752164 23.31069977478136 32-33 23.11252204233483 27.018279409295726 26.535174878992173 23.334023669377274 34-35 23.456471900630657 27.04672256468478 26.263815186798812 23.23299034788575 36-37 23.617465848411797 26.925102157081266 26.13836250630126 23.319069488205677 38-39 23.27799629295728 26.896133454964172 26.414025364558253 23.411844887520296 40-41 23.465835427294657 26.780003771447436 26.388422012847872 23.365738788410034 42-43 23.221665860194214 26.87677724696147 26.351955272413917 23.549601620430398 44-45 23.371290310277434 26.91761092756011 26.391368678971023 23.319730083191438 46-47 23.554098367667965 27.092591137068162 26.106850583169226 23.246459912094643 48-49 23.397956246530104 27.011409337958973 26.11974680769344 23.470887607817488 50-51 23.244542564194145 27.058487414787578 26.288387584655464 23.408582436362813 52-53 23.438786849396127 26.783256354435025 26.332753800898086 23.445202995270765 54-55 23.211160885995316 26.76406455359272 26.41524230767723 23.609532252734738 56-57 23.09293347427638 27.07733706044635 26.358755658975575 23.470973806301696 58-59 23.291938695321193 26.885738072877686 26.21039346264991 23.611929769151207 60-61 23.271415594656993 26.75858427700131 26.493006090526528 23.476994037815167 62-63 23.23461306124937 26.909828457982734 26.493339220199392 23.36221926056851 64-65 23.142380663567046 26.860123437009037 26.3409815040537 23.656514395370213 66-67 23.057353403002324 27.093055047104453 26.25314531712674 23.59644623276648 68-69 23.106602821008465 26.822109990276992 26.546087136311908 23.525200052402635 70-71 23.06794037984333 26.692636879639164 26.51481868764578 23.72460405287173 72-73 23.067784043516387 26.8277916004249 26.421001789418902 23.683422566639813 74-75 23.150730440089085 26.98030107306112 26.313968757752814 23.55499972909698 76-77 23.302105453576562 26.741301091356924 26.339619009767585 23.61697444529893 78-79 23.162093250145524 26.81881841989977 26.517629168821756 23.50145916113295 80-81 23.19640849213596 26.86132846509521 26.53048384799933 23.411779194769508 82-83 23.10477440438466 26.911265986031314 26.50900863971073 23.474950969873294 84-85 23.20847558712777 26.84890124874382 26.393291027646043 23.549332136482363 86-87 23.228532059123225 26.605879206578564 26.545029958540876 23.62055877575733 88-89 23.11015422893109 26.72642031505227 26.53306159622087 23.630363859795768 90-91 23.215578165203706 26.78271978419068 26.593997125940515 23.4077049246651 92-93 23.111071425834368 26.57964184513011 26.580426045209492 23.728860683826035 94-95 23.0413756868739 26.46547950713322 26.749639275356945 23.74350553063593 96-97 23.113820074648388 26.437138267046738 26.664359227623397 23.784682430681478 98-99 22.96262200558091 26.422160212083945 26.62139447224759 23.993823310087553 100-101 22.870650652199412 26.448968591257117 26.784791841277528 23.895588915265947 102-103 22.82115350921986 26.339454481192483 26.836999373858067 24.00239263572959 104-105 22.886861563027004 26.482751638242767 26.7311952118018 23.899191586928424 106-107 22.842204387723957 26.49986521911521 26.68987774161435 23.968052651546486 108-109 22.854708291442822 26.447065038371143 26.54407339199698 24.154153278189053 110-111 22.72351942790547 26.6221467410919 26.72111621454329 23.933217616459338 112-113 22.761618911399516 26.742783233915485 26.751785029283464 23.743812825401534 114-115 22.816818645674143 26.738422579846922 26.640082522925212 23.804676251553722 116-117 22.72274521257873 26.64522638011146 26.758561760471487 23.873466646838324 118-119 22.68248421280068 26.75285042431284 26.57633213269654 23.98833323018994 120-121 22.7313321008302 26.816346624465837 26.705164397491714 23.747156877212248 122-123 22.77433177233654 26.746094433086505 26.694221638014525 23.785352156562432 124-125 22.76021466695369 26.83201037862084 26.584220090841537 23.82355486358393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 818.0 1 684.5 2 528.5 3 446.0 4 338.5 5 272.5 6 246.5 7 243.5 8 268.0 9 288.0 10 273.0 11 259.0 12 274.5 13 328.0 14 383.0 15 425.0 16 526.5 17 647.0 18 845.5 19 1161.0 20 1561.5 21 2111.5 22 2941.0 23 4115.5 24 5689.0 25 7783.0 26 10773.0 27 14873.5 28 19045.5 29 23510.5 30 29220.0 31 34948.0 32 40308.0 33 44438.5 34 48648.5 35 52547.5 36 57206.5 37 63270.5 38 68075.5 39 71970.0 40 76216.0 41 82149.0 42 87532.5 43 91274.5 44 94179.0 45 97815.0 46 100841.0 47 102771.5 48 105819.0 49 107304.0 50 106256.5 51 104814.5 52 105838.5 53 108140.0 54 107298.5 55 101467.0 56 93573.5 57 87432.5 58 81864.5 59 73400.0 60 62389.0 61 51194.5 62 40882.5 63 31664.0 64 23770.5 65 17671.5 66 13214.5 67 10696.5 68 8066.0 69 5286.5 70 3571.5 71 2580.5 72 1942.0 73 1462.0 74 1048.5 75 718.5 76 500.0 77 306.5 78 161.0 79 89.0 80 61.0 81 54.5 82 44.5 83 31.0 84 27.5 85 27.5 86 25.5 87 19.0 88 15.0 89 13.5 90 11.0 91 9.0 92 9.0 93 7.0 94 5.5 95 5.5 96 4.0 97 3.0 98 2.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0647308252752486 2 0.05097196043150081 3 0.046979750943159496 4 0.04658765893984025 5 0.045304448747159116 6 0.004705104039830844 7 5.346709136171415E-4 8 0.0 9 8.554734617874263E-4 10-11 0.003421893847149705 12-13 0.002334729656128184 14-15 0.007930951885320931 16-17 0.0027981111145963735 18-19 0.004740748767405321 20-21 0.008911181893619023 22-23 0.016289640501535575 24-25 0.003404071483362467 26-27 0.016949067961663383 28-29 0.006986366604597314 30-31 0.006772498239150457 32-33 0.002388196747489898 34-35 0.01905210688855747 36-37 0.016753021960003764 38-39 0.028604893878517064 40-41 0.006237827325533316 42-43 0.0013010325564683774 44-45 2.495130930213327E-4 46-47 0.002156506018255804 48-49 0.0030832689351921825 50-51 0.0017287692873620907 52-53 0.0014792561943407579 54-55 0.0059526695049375074 56-57 0.002691176931872945 58-59 0.008180464978342263 60-61 0.016111416863663193 62-63 0.012457832287279395 64-65 0.002334729656128184 66-67 0.003671406940171038 68-69 0.009321096260725498 70-71 0.002512953294000565 72-73 0.0029941571162559917 74-75 0.0011406312823832349 76-77 4.0991436710647504E-4 78-79 0.003029801843830468 80-81 0.0024416638388516125 82-83 0.0016396574684259002 84-85 0.01158453646170473 86-87 0.006077426051448174 88-89 0.002013927107957899 90-91 8.732958255746642E-4 92-93 0.0020495718355323755 94-95 0.0 96-97 0.0016040127408514242 98-99 0.025860249855282406 100-101 0.01486385139855653 102-103 0.006754675875363219 104-105 0.014275713393577675 106-107 0.0325079915479222 108-109 0.0012653878288939014 110-111 0.001906992925234471 112-113 0.016681732504854812 114-115 0.032436702092773245 116-117 0.0497956844215431 118-119 0.3565542099274844 120-121 0.11789493645257967 122-123 0.018731304340387188 124-125 0.31887773288126314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2805464.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.2736923816135 #Duplication Level Percentage of deduplicated Percentage of total 1 84.62721411027822 63.702028820500246 2 9.631837595183109 14.500479604189465 3 2.865333922595089 6.470527927800742 4 1.1520619858574004 3.468798381119229 5 0.576997834933133 2.1716378765806827 6 0.3149432794882916 1.4224166122714912 7 0.20313778929832316 1.0703652023906098 8 0.13353901330644186 0.8041579686858636 9 0.08980187126054545 0.60837465893036 >10 0.3893120482083265 4.760259393543977 >50 0.013357334864585955 0.6883543769372857 >100 0.0023684814251697063 0.25287543136179846 >500 0.0 0.0 >1k 9.473330122388536E-5 0.07972374568826811 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.128945514895219E-5 0.0 0.0 0.0 0.0 6 7.128945514895219E-5 0.0 0.0 0.0 0.0 7 7.128945514895219E-5 0.0 0.0 0.0 0.0 8 7.128945514895219E-5 0.0 0.0 0.0 0.0 9 7.128945514895219E-5 0.0 0.0 0.0 0.0 10-11 7.128945514895219E-5 0.0 0.0 0.0 0.0 12-13 1.4257891029790439E-4 0.0 0.0 0.0 0.0 14-15 1.7822363787238046E-4 0.0 0.0 3.5644727574476097E-5 1.7822363787238048E-5 16-17 1.7822363787238046E-4 0.0 0.0 5.346709136171414E-5 7.128945514895219E-5 18-19 2.1386836544685657E-4 0.0 0.0 1.0693418272342828E-4 1.7822363787238046E-4 20-21 2.4951309302133264E-4 0.0 0.0 1.7822363787238046E-4 2.4951309302133264E-4 22-23 2.8515782059580877E-4 0.0 0.0 1.7822363787238046E-4 2.6733545680857073E-4 24-25 2.8515782059580877E-4 0.0 0.0 2.1386836544685657E-4 3.386249119575229E-4 26-27 2.8515782059580877E-4 0.0 0.0 6.416050963405696E-4 3.564472757447609E-4 28-29 3.386249119575229E-4 0.0 0.0 0.002013927107957899 3.564472757447609E-4 30-31 3.564472757447609E-4 0.0 0.0 0.007039833695959029 3.564472757447609E-4 32-33 3.564472757447609E-4 0.0 0.0 0.018945172705834044 4.2773673089371313E-4 34-35 3.9209200331923706E-4 0.0 0.0 0.03699922722230618 4.2773673089371313E-4 36-37 4.990261860426653E-4 0.0 0.0 0.06743982457090877 4.2773673089371313E-4 38-39 4.990261860426653E-4 0.0 1.7822363787238048E-5 0.11217395767687627 4.2773673089371313E-4 40-41 4.990261860426653E-4 0.0 3.5644727574476097E-5 0.15975966898880184 4.2773673089371313E-4 42-43 5.346709136171415E-4 0.0 3.5644727574476097E-5 0.20759489339374876 4.2773673089371313E-4 44-45 5.703156411916175E-4 0.0 3.5644727574476097E-5 0.268030528996273 4.2773673089371313E-4 46-47 6.416050963405697E-4 0.0 3.5644727574476097E-5 0.3411556876153107 4.2773673089371313E-4 48-49 6.772498239150458E-4 0.0 3.5644727574476097E-5 0.4181839439037535 4.2773673089371313E-4 50-51 6.772498239150458E-4 0.0 3.5644727574476097E-5 0.5003272185991336 4.2773673089371313E-4 52-53 6.772498239150458E-4 0.0 3.5644727574476097E-5 0.5820784012911946 4.633814584681892E-4 54-55 6.772498239150458E-4 0.0 3.5644727574476097E-5 0.6723308515097681 4.990261860426653E-4 56-57 6.772498239150458E-4 0.0 3.5644727574476097E-5 0.7799957511484732 4.990261860426653E-4 58-59 6.772498239150458E-4 0.0 3.5644727574476097E-5 0.9030948178269262 4.990261860426653E-4 60-61 6.772498239150458E-4 0.0 3.5644727574476097E-5 1.0354615136747434 4.990261860426653E-4 62-63 6.772498239150458E-4 0.0 3.5644727574476097E-5 1.170875833730178 4.990261860426653E-4 64-65 6.772498239150458E-4 0.0 3.5644727574476097E-5 1.3160211644134447 4.990261860426653E-4 66-67 7.485392790639979E-4 0.0 3.5644727574476097E-5 1.4836404958324185 4.990261860426653E-4 68-69 7.485392790639979E-4 0.0 3.5644727574476097E-5 1.6798112540385475 4.990261860426653E-4 70-71 7.841840066384741E-4 0.0 3.5644727574476097E-5 1.893608330030255 4.990261860426653E-4 72-73 8.376510980001883E-4 0.0 3.5644727574476097E-5 2.115122489541837 4.990261860426653E-4 74-75 8.732958255746642E-4 0.0 3.5644727574476097E-5 2.3417873121879307 4.990261860426653E-4 76-77 8.911181893619023E-4 0.0 3.5644727574476097E-5 2.596522357798924 4.990261860426653E-4 78-79 9.089405531491404E-4 0.0 3.5644727574476097E-5 2.882464362401371 4.990261860426653E-4 80-81 0.0010336970996598068 0.0 3.5644727574476097E-5 3.1970290832461226 4.990261860426653E-4 82-83 0.0010336970996598068 0.0 3.5644727574476097E-5 3.5280794905940693 4.990261860426653E-4 84-85 0.001087164191021521 0.0 3.5644727574476097E-5 3.8621418774220593 4.990261860426653E-4 86-87 0.001140631282383235 0.0 3.5644727574476097E-5 4.2160227327814574 5.168485498299033E-4 88-89 0.001158453646170473 0.0 3.5644727574476097E-5 4.609005854290057 5.346709136171414E-4 90-91 0.0012832101926811394 0.0 3.5644727574476097E-5 5.038685222836579 5.346709136171414E-4 92-93 0.0012832101926811394 0.0 3.5644727574476097E-5 5.483620534784977 5.346709136171414E-4 94-95 0.0012832101926811394 0.0 3.5644727574476097E-5 5.940568119925973 5.524932774043795E-4 96-97 0.0012832101926811394 0.0 3.5644727574476097E-5 6.406729867144971 5.703156411916175E-4 98-99 0.0012832101926811394 0.0 3.5644727574476097E-5 6.891409050338909 5.703156411916175E-4 100-101 0.0013010325564683774 0.0 3.5644727574476097E-5 7.439910118254948 5.703156411916175E-4 102-103 0.0013901443754045677 0.0 3.5644727574476097E-5 8.007463293059544 5.703156411916175E-4 104-105 0.0016040127408514242 0.0 3.5644727574476097E-5 8.58636931359661 5.703156411916175E-4 106-107 0.0016574798322131384 0.0 3.5644727574476097E-5 9.155651257688568 5.703156411916175E-4 108-109 0.0016931245597876145 0.0 3.5644727574476097E-5 9.757227324963 6.059603687660936E-4 110-111 0.0017109469235748525 0.0 3.5644727574476097E-5 10.395250126182336 6.059603687660936E-4 112-113 0.0017109469235748525 0.0 3.5644727574476097E-5 11.072286081731935 6.416050963405697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2630 0.0 43.437103 1 GTATCAA 3835 0.0 39.097645 1 ATCAACG 4040 0.0 35.341366 3 GTACATG 9580 0.0 34.96699 1 TCAACGC 4165 0.0 34.28131 4 CAACGCA 4150 0.0 34.26492 5 AACGCAG 4215 0.0 33.717876 6 TACATGG 9895 0.0 32.70785 2 ACATGGG 9915 0.0 31.62069 3 TATCAAC 4980 0.0 29.388313 2 CATGGGG 6440 0.0 29.007143 4 ACGCAGA 5460 0.0 25.703188 7 CGCAGAG 5535 0.0 25.462343 8 ATGGGGA 4115 0.0 21.543503 5 GAGTACA 7085 0.0 20.071205 1 GCAGAGT 7105 0.0 19.250372 9 GTACCGT 280 1.5767255E-6 19.113682 6 GTATAGG 1165 0.0 18.896929 1 CATGGGA 5005 0.0 18.66194 4 AGAGTAC 6105 0.0 18.117682 10-11 >>END_MODULE