FastQCFastQC Report
Thu 26 May 2016
SRR1364214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805464
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53860.19198250271612824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53110.18930914814804253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29500.10515194634470447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.068.7835541
GTATCAA71650.048.6106831
ATCAACG87050.039.3750533
TCAACGC90350.037.9368974
CAACGCA90800.037.8063285
AACGCAG92650.037.114326
TATCAAC93900.036.50462
GTACATG92150.031.4648511
ACGCAGA109850.031.3008357
CGCAGAG111600.030.7566998
TACATGG95800.029.1338582
TGGTATC19750.028.0224762
GTGGTAT22150.027.9544941
ACATGGG94200.027.8583343
GCAGAGT129000.026.100869
GAGTACT83850.025.78916212-13
CAGAGTA127650.024.37310-11
CATGGGG62100.024.051914
AGAGTAC120500.023.62234510-11
GTACTTT91400.023.43107414-15
Produced by FastQC (version 0.11.5)