FastQCFastQC Report
Thu 26 May 2016
SRR1364213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256666
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.053.0733641
GTATCAA35950.046.1751141
ATCAACG40050.040.253353
TCAACGC40450.039.7073444
CAACGCA40650.039.367395
AACGCAG42950.037.3820046
TATCAAC45550.035.9176942
GTACATG94100.035.1550561
TACATGG94500.033.9958652
ACATGGG95550.032.5616383
ACGCAGA52800.030.2956567
CGCAGAG54700.029.0259158
CATGGGG55750.026.9961174
CATGGGA48050.026.1224984
GCAGAGT65750.023.155519
GAGTACA70750.021.9490991
GTATAGG8150.021.1710821
AGTACAT69650.021.0979352
ATGGGAG26750.020.237765
AGAGTAC55150.020.00361610-11
Produced by FastQC (version 0.11.5)