FastQCFastQC Report
Thu 26 May 2016
SRR1364213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256666
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57950.2567947582850098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53930.23898086823659326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29870.13236340690204043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.072.861441
GTATCAA68350.055.3013881
ATCAACG88150.042.6582533
TCAACGC89950.041.870764
TATCAAC90550.041.4628222
CAACGCA90850.041.4513785
AACGCAG93600.040.4242026
ACGCAGA110700.034.017087
GTACATG87400.033.3041461
TGGTATC17000.033.2500722
CGCAGAG112450.033.2231758
TACATGG88700.032.2655752
ACATGGG89150.030.6337133
GTGGTAT19950.029.8371931
GCAGAGT126900.029.2525629
GAGTACT80850.026.15775912-13
CATGGGG55700.025.8504714
AGAGTAC114400.025.84468810-11
CAGAGTA120650.025.68924510-11
AGTACTT84100.024.72248512-13
Produced by FastQC (version 0.11.5)