FastQCFastQC Report
Thu 26 May 2016
SRR1364212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260044
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27410.12128082462111359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.053.6634521
GTATCAA43850.046.8101961
CAACGCA46950.040.4174735
ATCAACG47750.039.9885863
TCAACGC48150.039.4084364
GTACATG106400.038.862811
AACGCAG49400.038.4027636
TACATGG107650.037.355652
ACATGGG106150.036.536933
TATCAAC56650.034.127712
ACGCAGA59650.031.60237
CATGGGG49900.031.4701084
CGCAGAG60200.031.0172338
CATGGGA51200.029.9739934
GCAGAGT73550.024.5017499
GAGTACA74750.024.0373571
CATATAG7700.023.948343
TATAGGG7250.023.7948882
ATGGGAT24150.022.9076165
ATGGGGG22800.022.1767625
Produced by FastQC (version 0.11.5)