##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364212_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2260044 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.130715154218237 33.0 31.0 34.0 28.0 34.0 2 31.100049822038862 33.0 31.0 34.0 27.0 34.0 3 31.2274712350733 34.0 31.0 34.0 27.0 34.0 4 34.55870505176006 37.0 35.0 37.0 32.0 37.0 5 34.70590218597514 37.0 35.0 37.0 32.0 37.0 6 34.75384240306826 37.0 35.0 37.0 32.0 37.0 7 34.67092012367901 37.0 35.0 37.0 32.0 37.0 8 34.63499958407889 37.0 35.0 37.0 31.0 37.0 9 36.11146774133601 39.0 37.0 39.0 31.0 39.0 10-11 36.09395392302096 39.0 37.0 39.0 31.0 39.0 12-13 36.05819709704767 39.0 37.0 39.0 31.0 39.0 14-15 37.38179278810501 40.0 38.0 41.0 31.0 41.0 16-17 37.42337604931585 40.0 38.0 41.0 31.0 41.0 18-19 37.3482374679431 40.0 38.0 41.0 31.0 41.0 20-21 37.33995510706872 40.0 38.0 41.0 31.0 41.0 22-23 37.39645511326328 40.0 38.0 41.0 31.0 41.0 24-25 37.254266952324826 40.0 38.0 41.0 30.0 41.0 26-27 37.27959898125877 40.0 37.5 41.0 30.0 41.0 28-29 37.37675394815322 40.0 38.0 41.0 30.0 41.0 30-31 37.274269660236705 40.0 37.5 41.0 30.0 41.0 32-33 37.09032412643294 40.0 37.0 41.0 30.0 41.0 34-35 37.04934682687593 40.0 37.0 41.0 30.0 41.0 36-37 37.06705643783926 40.0 37.0 41.0 30.0 41.0 38-39 36.903762935588865 40.0 37.0 41.0 30.0 41.0 40-41 36.90701862441617 40.0 37.0 41.0 30.0 41.0 42-43 36.837712672850614 40.0 37.0 41.0 30.0 41.0 44-45 36.82739473213796 40.0 37.0 41.0 30.0 41.0 46-47 36.76325991883344 40.0 36.5 41.0 30.0 41.0 48-49 36.67869165379081 40.0 36.0 41.0 30.0 41.0 50-51 36.57735601607756 40.0 36.0 41.0 30.0 41.0 52-53 36.44839149149308 40.0 35.5 41.0 30.0 41.0 54-55 36.280387240248416 39.5 35.0 41.0 29.5 41.0 56-57 36.10452384997814 39.0 35.0 41.0 29.0 41.0 58-59 35.89057182072561 39.0 35.0 41.0 29.0 41.0 60-61 35.66601336080183 39.0 35.0 41.0 29.0 41.0 62-63 35.38196667852485 38.0 35.0 40.0 28.5 41.0 64-65 35.086023103974966 37.5 34.0 40.0 28.0 41.0 66-67 34.78483737484757 37.0 34.0 40.0 28.0 41.0 68-69 34.54441993164735 37.0 34.0 39.5 28.0 41.0 70-71 34.373328351129445 36.0 34.0 39.0 28.0 41.0 72-73 34.057242690850266 36.0 34.0 39.0 28.0 41.0 74-75 33.707849050726445 35.0 34.0 38.5 28.0 40.0 76-77 33.3747876147544 35.0 34.0 37.0 27.5 39.5 78-79 33.02488159522558 35.0 33.5 37.0 27.0 39.0 80-81 32.72038708095948 35.0 33.0 36.0 26.5 39.0 82-83 32.42851754213635 35.0 33.0 36.0 26.0 37.0 84-85 32.1661602605967 35.0 33.0 36.0 26.0 37.0 86-87 31.9630943025888 35.0 33.0 35.0 26.0 37.0 88-89 31.780399850622377 35.0 33.0 35.0 25.5 36.0 90-91 31.623523037604578 35.0 33.0 35.0 25.0 36.0 92-93 31.49736509554681 35.0 33.0 35.0 25.0 36.0 94-95 31.355516529766675 35.0 33.0 35.0 25.0 36.0 96-97 31.256719559442203 35.0 33.0 35.0 24.0 35.0 98-99 31.17896266621358 35.0 33.0 35.0 24.0 35.0 100-101 31.082523393349863 35.0 33.0 35.0 23.5 35.0 102-103 30.09800871133482 34.0 30.5 35.0 20.0 35.0 104-105 30.777047703496038 35.0 32.0 35.0 20.5 35.0 106-107 30.875376762576302 35.0 32.0 35.0 21.0 35.0 108-109 30.851198251007503 35.0 32.0 35.0 20.0 35.0 110-111 30.77108454525664 35.0 32.0 35.0 20.0 35.0 112-113 30.905898513480267 35.0 33.0 35.0 20.0 35.0 114-115 30.892792573949887 35.0 33.0 35.0 20.0 35.0 116-117 30.800521361531015 35.0 33.0 35.0 18.5 35.0 118-119 30.561407875244907 35.0 33.0 35.0 11.5 35.0 120-121 30.50032300256101 35.0 33.0 35.0 4.5 35.0 122-123 30.37004345933088 35.0 33.0 35.0 2.0 35.0 124-125 29.523513259033898 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5749558344038483 1101 2 0.5606828536148889 1101 3 0.5384424242014134 1101 4 0.5805303871197722 1101 5 0.5417220607234157 1101 6 0.5514061200051685 1101 7 0.5963923353022835 1101 8 0.4802908382392701 1101 9 0.5619264453304638 1101 10-11 0.6195736926207118 1101 12-13 0.5704603626756395 1101 14-15 0.6143994341467263 1101 16-17 0.6786169826999213 1101 18-19 0.7494468334249262 1101 20-21 0.7651413097521242 1101 22-23 0.7023362025273556 1101 24-25 0.9035680104557855 1101 26-27 0.9105851867551848 1101 28-29 0.7863943859529527 1101 30-31 0.8472818852682735 1101 32-33 1.018944576163527 1101 34-35 0.8645492287791896 1101 36-37 0.8237196544134378 1101 38-39 0.9146398554555049 1101 40-41 0.8186791886197682 1101 42-43 0.7534281486377665 1101 44-45 0.7409030516840858 1101 46-47 0.7473958856599552 1101 48-49 0.6448342059298895 1101 50-51 0.591738835576443 1101 52-53 0.5414251214784898 1101 54-55 0.5623195338939269 1101 56-57 0.5021373050895122 1101 58-59 0.5451374474470541 1101 60-61 0.4483809792402411 1101 62-63 0.38975279151056696 1101 64-65 0.3572053111544733 1101 66-67 0.3088731459937861 1101 68-69 0.3268062676941881 1101 70-71 0.36290690776381496 1101 72-73 0.3467270186832607 1101 74-75 0.3715399752904247 1101 76-77 0.40546649646232424 1101 78-79 0.3205335843940986 1101 80-81 0.3418142055793112 1101 82-83 0.38070397600833417 1101 84-85 0.3472563956994108 1101 86-87 0.32122776870419756 1101 88-89 0.26193376790721246 1101 90-91 0.2664182152548058 1101 92-93 0.30045984770576695 1101 94-95 0.3206392610349553 1101 96-97 0.3016360893690475 1101 98-99 0.23925912997382426 1101 100-101 0.1606727621557269 1101 102-103 0.07657715835453871 1101 104-105 0.18136198227811207 1101 106-107 0.1269098624548448 1101 108-109 0.06435983456098882 1101 110-111 -0.013143386975617233 1101 112-113 0.08045415015847013 1101 114-115 0.11420423845420657 1101 116-117 0.07719223106359863 1101 118-119 -0.07124529303692384 1101 120-121 -0.07237363592973622 1101 122-123 -0.0032571884533538764 1101 124-125 -0.17788480422771258 1102 1 0.22467628100256576 1102 2 0.26209540423684885 1102 3 0.2267097653824628 1102 4 0.3249022169880149 1102 5 0.25901679260585553 1102 6 0.2302390346283545 1102 7 0.2867627650190627 1102 8 0.3541595101364763 1102 9 0.3304176962913985 1102 10-11 0.28828469487353914 1102 12-13 0.3164991167315989 1102 14-15 0.35750654954858874 1102 16-17 0.307389618322901 1102 18-19 0.41931648962479073 1102 20-21 0.5199333948904865 1102 22-23 0.5207024127141224 1102 24-25 0.6661151034247013 1102 26-27 0.6498514234570791 1102 28-29 0.5224740381660169 1102 30-31 0.6022981090941215 1102 32-33 0.6765156948921671 1102 34-35 0.6488923307441681 1102 36-37 0.553397714955679 1102 38-39 0.5827022861871072 1102 40-41 0.550100189747134 1102 42-43 0.4501953057389585 1102 44-45 0.3064158333119593 1102 46-47 0.3527118830580349 1102 48-49 0.32177434087091683 1102 50-51 0.25897901114856836 1102 52-53 0.23543527734548775 1102 54-55 0.24730224183263516 1102 56-57 0.30490928069558976 1102 58-59 0.331492170287639 1102 60-61 0.340866515141542 1102 62-63 0.32615482192094447 1102 64-65 0.31296500777561675 1102 66-67 0.29206369821428524 1102 68-69 0.33036225181562173 1102 70-71 0.35051146607706585 1102 72-73 0.33425098244718754 1102 74-75 0.3765136604547408 1102 76-77 0.30916335413330387 1102 78-79 0.2872568103125417 1102 80-81 0.30837357181048475 1102 82-83 0.24391375646687408 1102 84-85 0.27631583346420285 1102 86-87 0.2666820453657337 1102 88-89 0.204578013074876 1102 90-91 0.15172730727374883 1102 92-93 0.22428044900787114 1102 94-95 0.2481387889130353 1102 96-97 0.21767783829938026 1102 98-99 0.26471699285436046 1102 100-101 0.10232278533262473 1102 102-103 0.026692470555886416 1102 104-105 0.18476059242749443 1102 106-107 0.24158159942475876 1102 108-109 0.26434934296285206 1102 110-111 0.2646720333845707 1102 112-113 0.27460384428069773 1102 114-115 0.3422310168277818 1102 116-117 0.30108942103210623 1102 118-119 0.17147515928448342 1102 120-121 0.2268880416958865 1102 122-123 0.17368733388325808 1102 124-125 0.16356463866842574 1103 1 0.29827362862475937 1103 2 0.06887599712962356 1103 3 0.26591274443264723 1103 4 0.36526866050120077 1103 5 0.31989125677846886 1103 6 0.2620514526519955 1103 7 0.3079072882959082 1103 8 0.29259606700811247 1103 9 0.3546727569125636 1103 10-11 0.4274884577233138 1103 12-13 0.3887198924293713 1103 14-15 0.4567458529304531 1103 16-17 0.4344641781190717 1103 18-19 0.4848296613768781 1103 20-21 0.5632450609444533 1103 22-23 0.5146632846739578 1103 24-25 0.7176078210662524 1103 26-27 0.666216441984389 1103 28-29 0.5206830561052769 1103 30-31 0.6067940122119069 1103 32-33 0.8105434173101216 1103 34-35 0.7848128813767588 1103 36-37 0.5727937916372738 1103 38-39 0.7478171252529862 1103 40-41 0.6455806005881186 1103 42-43 0.5764881210304722 1103 44-45 0.514258981249931 1103 46-47 0.4408493821523649 1103 48-49 0.3259361733102395 1103 50-51 0.4034334665617436 1103 52-53 0.2912932807708799 1103 54-55 0.1721128729843855 1103 56-57 0.19828443451626043 1103 58-59 0.22183516672288306 1103 60-61 0.21003440099890724 1103 62-63 0.23395991734471266 1103 64-65 0.22628551929469154 1103 66-67 0.1762704519285876 1103 68-69 0.24614967695559642 1103 70-71 0.20143369232024355 1103 72-73 0.268005340420693 1103 74-75 0.23347931607728611 1103 76-77 0.2469030462606483 1103 78-79 0.24416092810882617 1103 80-81 0.23629020000405632 1103 82-83 0.22018092621816976 1103 84-85 0.2526417403920149 1103 86-87 0.2791646273315749 1103 88-89 0.1628750000919048 1103 90-91 0.2835413693648263 1103 92-93 0.27238911192415216 1103 94-95 0.29525250427793637 1103 96-97 0.3188211533778329 1103 98-99 0.2660789880181227 1103 100-101 0.3353998831100924 1103 102-103 0.3797783660058158 1103 104-105 0.3407491367185784 1103 106-107 0.39174326286924455 1103 108-109 0.31984460011506854 1103 110-111 0.2992301396024146 1103 112-113 0.34273949475502263 1103 114-115 0.3348308972696685 1103 116-117 0.39557975917280785 1103 118-119 0.2830811474368744 1103 120-121 0.3024455573876459 1103 122-123 0.4462069475121204 1103 124-125 0.3483055804781898 1104 1 0.29733586892601593 1104 2 0.22336252990070804 1104 3 0.3536149827841264 1104 4 0.38230427928027666 1104 5 0.2703355597074122 1104 6 0.20253103533845973 1104 7 0.3573107541224658 1104 8 0.2880875849478315 1104 9 0.36622138660763426 1104 10-11 0.35096064962569784 1104 12-13 0.3032985046345047 1104 14-15 0.3512559315623207 1104 16-17 0.3924744405423155 1104 18-19 0.4833473822266683 1104 20-21 0.5453384204919018 1104 22-23 0.5448700478349906 1104 24-25 0.6931240101783942 1104 26-27 0.6605485176407484 1104 28-29 0.5732370137034977 1104 30-31 0.5549126234859472 1104 32-33 0.6562623824385057 1104 34-35 0.6928584026126359 1104 36-37 0.5607462452212673 1104 38-39 0.6103361743894027 1104 40-41 0.5521208985024728 1104 42-43 0.40606276751172743 1104 44-45 0.38887751060090636 1104 46-47 0.41122712413555007 1104 48-49 0.33610420175948974 1104 50-51 0.3387180985272451 1104 52-53 0.29645833742632277 1104 54-55 0.3583106782077934 1104 56-57 0.27709966224858107 1104 58-59 0.30350304041798637 1104 60-61 0.28316989908782375 1104 62-63 0.29682299576513316 1104 64-65 0.30544448117852596 1104 66-67 0.31174326418043563 1104 68-69 0.29087191300884996 1104 70-71 0.2781927986095525 1104 72-73 0.2775067978515935 1104 74-75 0.3145380743863342 1104 76-77 0.2596702399419186 1104 78-79 0.3388487145837047 1104 80-81 0.22115985188195708 1104 82-83 0.22684906410444938 1104 84-85 0.3318199334899745 1104 86-87 0.3927008601290396 1104 88-89 0.43743571203526344 1104 90-91 0.36807298029222935 1104 92-93 0.3283907406747062 1104 94-95 0.3608498424494151 1104 96-97 0.3422385363199538 1104 98-99 0.29027076054294554 1104 100-101 0.26150475206317125 1104 102-103 0.2890302651120109 1104 104-105 0.34127347291561705 1104 106-107 0.2986460025270645 1104 108-109 0.26451349335012964 1104 110-111 0.31811000325501126 1104 112-113 0.4368726701512138 1104 114-115 0.4988615864792507 1104 116-117 0.44230304113753505 1104 118-119 0.20474517679323156 1104 120-121 0.16859749711740335 1104 122-123 0.14588740470472317 1104 124-125 0.2016603372571204 1105 1 0.3791005041366624 1105 2 0.3912417397796375 1105 3 0.4681959988895805 1105 4 0.34570692929124647 1105 5 0.354437034148134 1105 6 0.27588937847436057 1105 7 0.1975430359232675 1105 8 0.28356756115843496 1105 9 0.380031636905116 1105 10-11 0.3433196436935617 1105 12-13 0.4042959717595451 1105 14-15 0.370981513282004 1105 16-17 0.41380998901713895 1105 18-19 0.5849279622926531 1105 20-21 0.577891180007498 1105 22-23 0.5165427917648131 1105 24-25 0.8538453955199472 1105 26-27 0.7537650933390552 1105 28-29 0.5788709871806077 1105 30-31 0.5357632467815421 1105 32-33 0.6807110716269378 1105 34-35 0.6716440228878824 1105 36-37 0.6247253989686357 1105 38-39 0.6672875406324081 1105 40-41 0.6204356624292515 1105 42-43 0.5751644001812863 1105 44-45 0.48265666216387615 1105 46-47 0.46413562688653087 1105 48-49 0.39630434676991655 1105 50-51 0.3016683260827122 1105 52-53 0.32568217690181456 1105 54-55 0.33222504401400954 1105 56-57 0.4258895196960637 1105 58-59 0.4418598297373606 1105 60-61 0.3604105431193432 1105 62-63 0.3986130073340419 1105 64-65 0.416265065266046 1105 66-67 0.38899716396117867 1105 68-69 0.38786945780137927 1105 70-71 0.4275948943061678 1105 72-73 0.39762180921279366 1105 74-75 0.40108327420414724 1105 76-77 0.3464613245730632 1105 78-79 0.4033706072470409 1105 80-81 0.3523718390373176 1105 82-83 0.4038405747305731 1105 84-85 0.44779327001712943 1105 86-87 0.35749452365686096 1105 88-89 0.42683381980506496 1105 90-91 0.3694121843666345 1105 92-93 0.33663712360548104 1105 94-95 0.3539013093894354 1105 96-97 0.29122341197682644 1105 98-99 0.3588165665779144 1105 100-101 0.3371344024857237 1105 102-103 0.3042968741690686 1105 104-105 0.2882473954029017 1105 106-107 0.37362598121144686 1105 108-109 0.3750051574908895 1105 110-111 0.3447620873528443 1105 112-113 0.3809364422538941 1105 114-115 0.4017854406947592 1105 116-117 0.43762548863800177 1105 118-119 0.3423465751067454 1105 120-121 0.33762206907572434 1105 122-123 0.38222215083332145 1105 124-125 0.4218387054986614 1106 1 0.20642162292394772 1106 2 0.2387508342925173 1106 3 0.24124614809019107 1106 4 0.27575519270178717 1106 5 0.17177536162877516 1106 6 0.23852117785149574 1106 7 0.18349068269267832 1106 8 0.25116224310728086 1106 9 0.3074813278259896 1106 10-11 0.37219228463765575 1106 12-13 0.3281675609769792 1106 14-15 0.350309886082826 1106 16-17 0.322936789948443 1106 18-19 0.34474688146748633 1106 20-21 0.4784161776804865 1106 22-23 0.44085176005264515 1106 24-25 0.5368326704752064 1106 26-27 0.49871254404198595 1106 28-29 0.3812224998419751 1106 30-31 0.4495130489971402 1106 32-33 0.6565706651812135 1106 34-35 0.6227629627759725 1106 36-37 0.5051468000041908 1106 38-39 0.5320127356569699 1106 40-41 0.42727903404804835 1106 42-43 0.4193842273839792 1106 44-45 0.3109410679713349 1106 46-47 0.30934908366887015 1106 48-49 0.3018218556315091 1106 50-51 0.3015644474379613 1106 52-53 0.23347185263177295 1106 54-55 0.21757479064917362 1106 56-57 0.24715622695632078 1106 58-59 0.2390603359583352 1106 60-61 0.28338466189778444 1106 62-63 0.16143102078346772 1106 64-65 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-1.8703494202256792 2216 68-69 -2.047838268482863 2216 70-71 -2.0575809949996327 2216 72-73 -2.0031841153610017 2216 74-75 -2.0865898263378284 2216 76-77 -2.1612732696195494 2216 78-79 -2.1203676883957243 2216 80-81 -2.0734850720264646 2216 82-83 -2.1063574275004413 2216 84-85 -2.201730245740791 2216 86-87 -2.157321136237897 2216 88-89 -2.057533609687102 2216 90-91 -2.1210172852611926 2216 92-93 -2.194335760596033 2216 94-95 -2.0916586405818336 2216 96-97 -2.208063199703304 2216 98-99 -2.1820514995411386 2216 100-101 -2.2211211834927056 2216 102-103 -2.2033792686607114 2216 104-105 -2.2182653540365287 2216 106-107 -2.2608037025033028 2216 108-109 -2.1219060753117347 2216 110-111 -2.1685183439759577 2216 112-113 -2.1874939062570746 2216 114-115 -2.1782865882858466 2216 116-117 -2.1825357244460903 2216 118-119 -2.1019943470444886 2216 120-121 -2.00470221063447 2216 122-123 -1.9809418003097896 2216 124-125 -1.9560366790127155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33108.0 3 6322.0 4 4563.0 5 4177.0 6 4898.0 7 5205.0 8 5139.0 9 5171.0 10 5675.0 11 6799.0 12 7778.0 13 7812.0 14 7819.0 15 7712.0 16 7244.0 17 6571.0 18 6003.0 19 5776.0 20 5571.0 21 5752.0 22 6561.0 23 8842.0 24 13654.0 25 20599.0 26 26114.0 27 24111.0 28 26149.0 29 32444.0 30 38563.0 31 46360.0 32 60465.0 33 81423.0 34 116296.0 35 182988.0 36 363069.0 37 723909.0 38 338436.0 39 966.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.481233081348556 19.915638080547172 12.903424330625759 23.69970450747852 2 15.41188204633521 20.960632382230717 40.13507537118944 23.492410200244635 3 18.30334119676084 26.784864503065762 29.431456041462944 25.480338258710454 4 12.036789876542226 17.42472634906469 38.43946257422009 32.099021200173 5 12.815630938726619 39.46043549223177 34.75589053066236 12.968043038379252 6 30.330291662352398 38.40425993233455 18.10360619874315 13.161842206569904 7 27.566097369202364 33.385117146312204 22.093886073546585 16.954899410938847 8 25.25972945659465 36.53632407156675 20.356462086578844 17.847484385259758 9 26.272408491056513 16.993794316434553 20.464729372462696 36.26906782004624 10-11 24.07377539721257 27.783386265510458 28.38319577604545 19.759642561231523 12-13 24.405174885574816 25.793144400511153 27.873240259257553 21.928440454656474 14-15 22.257138185573023 27.05612998620939 25.12157223168319 25.565159596534404 16-17 21.944437131205337 29.89218269803655 26.317505836886646 21.845874333871464 18-19 22.016269933367568 28.898147756871822 27.685812272075903 21.399770037684704 20-21 22.566414170244364 28.17450308296981 27.54191237055465 21.717170376231174 22-23 22.58541887198587 28.03455609800999 27.13593646556516 22.244088564438986 24-25 22.240467298135087 28.406299993738855 27.587948603753897 21.76528410437216 26-27 21.92434232726016 28.371284310269786 27.561284361161693 22.143089001308365 28-29 21.94133772293723 28.194244397391905 27.732937737982244 22.131480141688627 30-31 22.112784935930623 28.13448329877419 27.43312296948441 22.319608795810776 32-33 21.898144501780102 28.098514769560516 27.486984432646928 22.516356296012454 34-35 22.484738759965055 28.279031317294834 27.39214461026196 21.84408531247815 36-37 22.730921633168208 27.671520100402443 27.35656762428387 22.240990642145483 38-39 22.12799481284529 28.37561727472999 27.10356937764102 22.3928185347837 40-41 22.302205387801337 28.065528868278523 27.62411943785621 22.00814630606393 42-43 22.199955885219268 27.9716470720197 27.405809744056835 22.422587298704187 44-45 22.049199150988102 28.222787305882775 27.57684712520448 22.151166417924646 46-47 22.475089502568814 28.313962312239298 26.94535066732567 22.26559751786622 48-49 22.026450723370512 28.377204005706258 27.110708255308907 22.485637015614326 50-51 21.999806195033283 28.278489772584475 27.23223056242378 22.489473469958458 52-53 22.429888553541158 28.097255364912264 27.24369681713657 22.229159264410008 54-55 22.14786839328028 28.284249628857722 27.32111605967481 22.24676591818719 56-57 21.92187601286646 28.521412425616194 27.246182440631785 22.310529120885565 58-59 22.04883954582333 27.924864687983092 27.319188121260986 22.707107644932595 60-61 21.91838109268632 28.35492988661754 27.429119921830225 22.29756909886591 62-63 21.947913559803556 28.05363526216246 27.736016128989434 22.262435049044555 64-65 21.879410970845004 28.07459325041261 27.409877919813802 22.63611785892858 66-67 21.800560850014104 28.30750510240767 27.101555888648594 22.790378158929627 68-69 22.003974654607294 27.922634511879046 27.52873128334614 22.544659550167516 70-71 22.013568629099435 27.889605828337928 27.4919496414258 22.60487590113684 72-73 22.140502974924264 27.980062598079137 27.31098395622781 22.568450470768795 74-75 21.77041040272139 28.457131428692794 27.2316577169092 22.540800451676617 76-77 22.042876703206606 28.185161447470918 27.44574926489084 22.326212584431637 78-79 22.273901763050866 28.080206214716142 27.18656781304391 22.459324209189084 80-81 22.06917302548468 28.038637424584678 27.238034765717472 22.65415478421317 82-83 22.23915430443987 28.2314300006261 27.287761401002957 22.241654293931067 84-85 22.042557767305855 28.22366059990291 27.26793400652005 22.465847626271184 86-87 21.894120046957852 27.94644804922698 27.55390838263126 22.605523521183912 88-89 22.238579118187793 27.853168466441446 27.391067718876727 22.517184696494034 90-91 22.055134739540723 28.082629101333744 27.477923936306958 22.38431222281858 92-93 22.138365030334164 27.978648606433737 27.490505501565714 22.392480861666385 94-95 22.033885180996478 27.569839348260476 27.6529350756003 22.74334039514275 96-97 22.299859469772727 27.426248179213207 27.488570857145888 22.785321493868175 98-99 21.808847513061487 27.62695811693269 27.410760321397827 23.15343404860799 100-101 21.799160115510116 27.72397675725108 27.604382056059713 22.87248107117909 102-103 21.865950233627647 27.577000658657607 27.581713257602825 22.975335850111918 104-105 21.771173296101995 27.621236024418682 27.557224377086797 23.05036630239253 106-107 21.77361458560171 27.847710200042137 27.376728614399703 23.001946599956447 108-109 21.796375073118888 28.077475489133274 26.956400347253872 23.16974909049397 110-111 21.67804458882954 27.928184245569316 27.261991176570145 23.131779989031003 112-113 21.797718798098497 27.909386525384498 27.60655684019815 22.686337836318852 114-115 21.624539852318986 28.346978239999576 27.31215772470499 22.71632418297645 116-117 21.62060163583466 28.276051968473265 27.229063777719148 22.874282617972934 118-119 21.495899418973334 28.137139878039463 27.317623172856763 23.049337530130433 120-121 21.43145720388876 28.234327613718218 27.432711756737245 22.901503425655783 122-123 21.56975422954906 28.37861071951181 27.341196494879533 22.7104385560596 124-125 21.40987061689025 28.12692124840252 27.435194141249408 23.028013993457826 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1995.0 1 1591.5 2 1126.0 3 914.5 4 673.0 5 529.0 6 467.5 7 477.0 8 510.5 9 513.5 10 455.5 11 401.0 12 400.5 13 433.5 14 491.0 15 540.0 16 589.5 17 695.5 18 882.0 19 1093.0 20 1384.5 21 1961.0 22 2821.5 23 3896.0 24 5220.0 25 7399.0 26 11510.0 27 15939.0 28 18718.5 29 21932.5 30 27510.5 31 34637.0 32 42985.0 33 50474.0 34 57822.0 35 64590.0 36 72264.0 37 80117.0 38 82439.0 39 83748.5 40 82819.5 41 81142.5 42 80980.5 43 80080.5 44 79029.0 45 79742.0 46 78281.5 47 73216.5 48 69912.5 49 68637.5 50 68510.0 51 67044.0 52 65634.5 53 65801.0 54 66171.5 55 63641.5 56 58638.0 57 54251.5 58 50912.5 59 45808.0 60 38301.5 61 31626.5 62 26294.0 63 19859.0 64 14326.0 65 11383.0 66 8892.0 67 6971.0 68 5419.0 69 4151.0 70 3147.5 71 2180.0 72 1610.5 73 1340.5 74 1008.5 75 711.0 76 475.5 77 258.0 78 163.0 79 115.0 80 77.5 81 53.5 82 50.5 83 38.0 84 26.0 85 27.5 86 21.0 87 19.0 88 20.0 89 14.5 90 10.0 91 10.0 92 9.0 93 7.5 94 6.5 95 4.5 96 1.5 97 1.5 98 3.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06380406753142859 2 0.051193693574107406 3 0.047034482514499716 4 0.04659201325283933 5 0.04588406243418269 6 0.004513186468936003 7 3.0972848316227473E-4 8 0.0 9 8.406915971547456E-4 10-11 0.003384889851702002 12-13 0.002300840160634041 14-15 0.007676841689807809 16-17 0.002809679811543492 18-19 0.0048007914890152575 20-21 0.009291854494868242 22-23 0.01575190571510997 24-25 0.003252149073203884 26-27 0.015597041473528833 28-29 0.0070352612604002406 30-31 0.006637038924905887 32-33 0.0025663217176302763 34-35 0.01761027661408362 36-37 0.014955461044121265 38-39 0.027278229981363193 40-41 0.005486618844588867 42-43 0.001349531248064197 44-45 2.2123463083019624E-4 46-47 0.00234508708680008 48-49 0.0030087909792906684 50-51 0.00176987704664157 52-53 0.0015486424158113736 54-55 0.005707853475419062 56-57 0.002632692106879335 58-59 0.007721088615973848 60-61 0.015353683379615618 62-63 0.011592694655502283 64-65 0.002389334012966119 66-67 0.0036061244825321983 68-69 0.00995555838735883 70-71 0.0022787166975510214 72-73 0.003119408294705767 74-75 8.84938523320785E-4 76-77 2.4335809391321585E-4 78-79 0.0030751613685397277 80-81 0.0026105686437963155 82-83 0.001526518952728354 84-85 0.010840496910679615 86-87 0.0064158042940756915 88-89 0.0017256301204755305 90-91 7.521977448226671E-4 92-93 0.0022565932344680013 94-95 0.0 96-97 0.0014159016373132558 98-99 0.023849093203495153 100-101 0.014535115245543892 102-103 0.006393680830992672 104-105 0.013229830923645734 106-107 0.031857786839548256 108-109 0.0011504200803170205 110-111 0.001703506657392511 112-113 0.01584039956744205 114-115 0.03163655220871806 116-117 0.050264508124620584 118-119 0.3614088929242086 120-121 0.11796230515866064 122-123 0.019269536345310093 124-125 0.32198488171026757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2260044.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.675056750660694 #Duplication Level Percentage of deduplicated Percentage of total 1 83.44103267869444 33.105277068609354 2 8.490463963245578 6.737192791624157 3 2.6840799254668077 3.194730700986142 4 1.2340711944810827 1.9584737870157034 5 0.694788872707335 1.3782893977195545 6 0.4866221923044904 1.1584057857486947 7 0.336530118225968 0.9346296077245284 8 0.24973658264087553 0.7926650471194242 9 0.20210350018134968 0.7216621055281991 >10 1.5777791461065303 12.882759545871872 >50 0.31059998120627874 8.743120617577912 >100 0.26472581133380024 20.23128116234804 >500 0.02366753942388108 6.003062351689676 >1k 0.0037984939816105437 2.1584500304368244 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2741 0.12128082462111359 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.424692616603924E-5 0.0 0.0 0.0 0.0 6 4.424692616603924E-5 0.0 0.0 0.0 0.0 7 4.424692616603924E-5 0.0 0.0 0.0 0.0 8 4.424692616603924E-5 0.0 0.0 0.0 0.0 9 4.424692616603924E-5 0.0 0.0 0.0 0.0 10-11 4.424692616603924E-5 0.0 0.0 0.0 0.0 12-13 4.424692616603924E-5 0.0 0.0 0.0 0.0 14-15 4.424692616603924E-5 0.0 0.0 0.0 0.0 16-17 4.424692616603924E-5 0.0 0.0 6.637038924905886E-5 0.0 18-19 6.637038924905886E-5 0.0 0.0 2.6548155699623546E-4 0.0 20-21 8.849385233207849E-5 0.0 0.0 4.203457985773728E-4 0.0 22-23 8.849385233207849E-5 0.0 0.0 7.521977448226671E-4 0.0 24-25 8.849385233207849E-5 0.0 0.0 0.0013274077849811773 0.0 26-27 1.1061731541509811E-4 0.0 0.0 0.0019247412882227072 0.0 28-29 2.2123463083019622E-4 0.0 0.0 0.00411496413344165 0.0 30-31 2.2123463083019622E-4 0.0 0.0 0.012610373957321185 0.0 32-33 2.2123463083019622E-4 0.0 0.0 0.028826872397174567 0.0 34-35 2.4335809391321585E-4 0.0 0.0 0.05398124992256788 0.0 36-37 2.6548155699623546E-4 0.0 0.0 0.09110442097587482 0.0 38-39 3.5397540932831395E-4 0.0 0.0 0.14413436198587284 0.0 40-41 3.7609887241133355E-4 0.0 0.0 0.20402257655160697 0.0 42-43 3.982223354943532E-4 0.0 0.0 0.26508333466074113 0.0 44-45 3.982223354943532E-4 0.0 0.0 0.34059071416308706 0.0 46-47 4.645927247434121E-4 0.0 0.0 0.4322482217160374 0.0 48-49 4.867161878264317E-4 2.212346308301962E-5 0.0 0.5299896816168181 0.0 50-51 4.867161878264317E-4 4.424692616603924E-5 0.0 0.6374875887372105 0.0 52-53 4.867161878264317E-4 4.424692616603924E-5 0.0 0.7385475681004441 0.0 54-55 4.867161878264317E-4 4.424692616603924E-5 0.0 0.8490985131262931 0.0 56-57 4.867161878264317E-4 4.424692616603924E-5 0.0 0.9818835385505769 0.0 58-59 5.752100401585102E-4 4.424692616603924E-5 0.0 1.1291373088311554 0.0 60-61 5.752100401585102E-4 4.424692616603924E-5 0.0 1.2983818014162556 0.0 62-63 5.752100401585102E-4 4.424692616603924E-5 0.0 1.4666307381626198 0.0 64-65 6.194569663245495E-4 4.424692616603924E-5 0.0 1.63704777429112 0.0 66-67 6.194569663245495E-4 4.424692616603924E-5 0.0 1.8373757325078626 0.0 68-69 6.194569663245495E-4 4.424692616603924E-5 0.0 2.068720785966999 0.0 70-71 6.415804294075691E-4 4.424692616603924E-5 0.0 2.3278086621322416 0.0 72-73 6.637038924905887E-4 4.424692616603924E-5 0.0 2.590082316981439 0.0 74-75 6.637038924905887E-4 4.424692616603924E-5 0.0 2.8572452571719844 0.0 76-77 6.637038924905887E-4 4.424692616603924E-5 0.0 3.1536775390213645 0.0 78-79 6.637038924905887E-4 4.424692616603924E-5 0.0 3.4974097849422403 0.0 80-81 7.521977448226672E-4 4.424692616603924E-5 0.0 3.876274090238951 0.0 82-83 7.521977448226672E-4 4.424692616603924E-5 0.0 4.276399043558444 0.0 84-85 7.964446709887064E-4 4.424692616603924E-5 0.0 4.662785326303382 0.0 86-87 8.849385233207849E-4 4.424692616603924E-5 0.0 5.07664895019743 0.0 88-89 8.849385233207849E-4 4.424692616603924E-5 0.0 5.520622607347468 0.0 90-91 0.0010398027649019222 4.424692616603924E-5 0.0 6.0174934647290055 0.0 92-93 0.0010619262279849418 4.424692616603924E-5 0.0 6.538257662240204 0.0 94-95 0.0010619262279849418 4.424692616603924E-5 0.0 7.063380181978758 0.0 96-97 0.0010619262279849418 4.424692616603924E-5 0.0 7.6037015208553465 0.0 98-99 0.0010840496910679614 4.424692616603924E-5 0.0 8.167031261338275 0.0 100-101 0.0011504200803170205 4.424692616603924E-5 0.0 8.789497018642116 0.0 102-103 0.0011946670064830597 4.424692616603924E-5 0.0 9.453444269226617 0.0 104-105 0.001460148563479295 4.424692616603924E-5 0.0 10.12378520064211 0.0 106-107 0.001460148563479295 4.424692616603924E-5 0.0 10.79027664948116 0.0 108-109 0.0015486424158113736 4.424692616603924E-5 0.0 11.465772347795 0.0 110-111 0.0015486424158113736 4.424692616603924E-5 0.0 12.195758135682315 0.0 112-113 0.0015486424158113736 4.424692616603924E-5 0.0 12.97366334460745 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2295 0.0 53.663452 1 GTATCAA 4385 0.0 46.810196 1 CAACGCA 4695 0.0 40.417473 5 ATCAACG 4775 0.0 39.988586 3 TCAACGC 4815 0.0 39.408436 4 GTACATG 10640 0.0 38.86281 1 AACGCAG 4940 0.0 38.402763 6 TACATGG 10765 0.0 37.35565 2 ACATGGG 10615 0.0 36.53693 3 TATCAAC 5665 0.0 34.12771 2 ACGCAGA 5965 0.0 31.6023 7 CATGGGG 4990 0.0 31.470108 4 CGCAGAG 6020 0.0 31.017233 8 CATGGGA 5120 0.0 29.973993 4 GCAGAGT 7355 0.0 24.501749 9 GAGTACA 7475 0.0 24.037357 1 CATATAG 770 0.0 23.94834 3 TATAGGG 725 0.0 23.794888 2 ATGGGAT 2415 0.0 22.907616 5 ATGGGGG 2280 0.0 22.176762 5 >>END_MODULE