FastQCFastQC Report
Thu 26 May 2016
SRR1364212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260044
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82550.36525837550065393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73120.323533524126079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43430.19216440033910845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44950.077.8960651
GTATCAA90600.054.7500721
ATCAACG112050.043.3349763
CAACGCA112400.043.1933445
TCAACGC113000.042.9706544
AACGCAG116750.041.6831556
TATCAAC118900.040.7418982
ACGCAGA135150.035.7865147
CGCAGAG135950.035.4446568
GTACATG105800.035.0647131
TACATGG105650.034.867422
ACATGGG103300.034.6205863
TGGTATC19300.034.5349922
GCAGAGT151800.031.3910399
CATGGGG48400.031.228324
CAGAGTA144400.028.09714110-11
CATATAG8050.028.0897563
GAGTACT99500.027.68295712-13
GTACTTT104650.026.20693614-15
AGAGTAC141200.026.14279610-11
Produced by FastQC (version 0.11.5)