FastQCFastQC Report
Thu 26 May 2016
SRR1364211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211151
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.054.7960971
GTATCAA32850.044.01431
TCAACGC37350.036.7913744
CAACGCA37300.036.682875
ATCAACG37650.036.341863
AACGCAG38800.035.4060256
TATCAAC42650.033.4784972
GTACATG84050.033.2015271
TACATGG85800.031.4112112
ACATGGG87300.030.3923933
ACGCAGA49800.026.8683057
CGCAGAG51050.025.8609458
CATGGGG54400.024.4947954
CATGGGA42450.022.5617524
GTGTAGG11650.020.94021
GCAGAGT62600.020.4258779
ATGGGGA34050.020.26685
TGGTATC13750.019.9034752
CAGAGTA59500.019.34107410-11
GAGTACA67900.018.9280931
Produced by FastQC (version 0.11.5)