##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364211_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2211151 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.185484392517743 33.0 31.0 34.0 28.0 34.0 2 31.16603750716256 33.0 31.0 34.0 28.0 34.0 3 31.239002673268356 34.0 31.0 34.0 27.0 34.0 4 34.63171940767501 37.0 35.0 37.0 32.0 37.0 5 34.70037731480121 37.0 35.0 37.0 32.0 37.0 6 34.76326401950839 37.0 35.0 37.0 32.0 37.0 7 34.642129370630954 37.0 35.0 37.0 32.0 37.0 8 34.624240497369925 37.0 35.0 37.0 32.0 37.0 9 36.14174834735393 39.0 37.0 39.0 32.0 39.0 10-11 36.09192791446627 39.0 37.0 39.0 31.0 39.0 12-13 36.07817874039358 39.0 37.0 39.0 31.0 39.0 14-15 37.416686603492934 40.0 38.0 41.0 31.5 41.0 16-17 37.46496010448857 40.0 38.0 41.0 31.5 41.0 18-19 37.35793304030344 40.0 38.0 41.0 31.0 41.0 20-21 37.32084172451361 40.0 38.0 41.0 31.0 41.0 22-23 37.361232000890034 40.0 38.0 41.0 31.0 41.0 24-25 37.21903185264145 40.0 38.0 41.0 30.0 41.0 26-27 37.23721469044855 40.0 37.5 41.0 30.0 41.0 28-29 37.327062240434955 40.0 38.0 41.0 30.0 41.0 30-31 37.2270819586722 40.0 37.0 41.0 30.0 41.0 32-33 37.04361619807965 40.0 37.0 41.0 30.0 41.0 34-35 37.00324129831024 40.0 37.0 41.0 30.0 41.0 36-37 37.03730726666791 40.0 37.0 41.0 30.0 41.0 38-39 36.88431522768006 40.0 37.0 41.0 30.0 41.0 40-41 36.89042901185853 40.0 37.0 41.0 30.0 41.0 42-43 36.83264100913958 40.0 37.0 41.0 30.0 41.0 44-45 36.82762959200887 40.0 37.0 41.0 30.0 41.0 46-47 36.75734786995551 40.0 36.5 41.0 30.0 41.0 48-49 36.67244095948219 40.0 36.0 41.0 30.0 41.0 50-51 36.55872688025377 40.0 36.0 41.0 30.0 41.0 52-53 36.41924929595491 40.0 35.5 41.0 30.0 41.0 54-55 36.23571117485871 39.5 35.0 41.0 29.0 41.0 56-57 36.04840601116794 39.0 35.0 41.0 29.0 41.0 58-59 35.824715272724475 39.0 35.0 41.0 29.0 41.0 60-61 35.587037701179156 38.5 35.0 40.5 29.0 41.0 62-63 35.292894515119045 38.0 34.5 40.0 28.0 41.0 64-65 34.987773110022786 37.0 34.0 40.0 28.0 41.0 66-67 34.679162345764716 37.0 34.0 40.0 28.0 41.0 68-69 34.447516926704694 36.5 34.0 39.0 28.0 41.0 70-71 34.269960531867795 36.0 34.0 39.0 28.0 41.0 72-73 33.9443859781625 35.5 34.0 39.0 28.0 40.5 74-75 33.60513528926789 35.0 34.0 37.5 28.0 40.0 76-77 33.27601574926362 35.0 34.0 37.0 27.0 39.0 78-79 32.934729468950785 35.0 33.5 37.0 27.0 39.0 80-81 32.64046191327503 35.0 33.0 36.0 26.0 38.5 82-83 32.37448505325959 35.0 33.0 36.0 26.0 37.0 84-85 32.132324070133606 35.0 33.0 35.5 26.0 37.0 86-87 31.930395074782318 35.0 33.0 35.0 26.0 36.5 88-89 31.766470268199683 35.0 33.0 35.0 25.5 36.0 90-91 31.626584073181796 35.0 33.0 35.0 25.0 36.0 92-93 31.50045768018557 35.0 33.0 35.0 25.0 36.0 94-95 31.378125691099342 35.0 33.0 35.0 25.0 35.5 96-97 31.28875391142441 35.0 33.0 35.0 24.0 35.0 98-99 31.207545074940608 35.0 33.0 35.0 24.0 35.0 100-101 31.106566670480667 35.0 32.5 35.0 23.5 35.0 102-103 30.14582608786103 34.0 30.5 35.0 20.0 35.0 104-105 30.820989611292944 35.0 32.0 35.0 23.0 35.0 106-107 30.914359534920955 35.0 32.0 35.0 23.0 35.0 108-109 30.874143602133007 35.0 32.0 35.0 20.5 35.0 110-111 30.800452343598423 35.0 32.0 35.0 20.0 35.0 112-113 30.945976552483298 35.0 33.0 35.0 22.5 35.0 114-115 30.934396836760584 35.0 33.0 35.0 20.0 35.0 116-117 30.84024451518689 35.0 33.0 35.0 19.5 35.0 118-119 30.59257554097391 35.0 33.0 35.0 15.0 35.0 120-121 30.53223796113427 35.0 33.0 35.0 8.5 35.0 122-123 30.39184004167965 35.0 33.0 35.0 2.0 35.0 124-125 29.5232428721512 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47816039427293333 1101 2 0.5137906079087067 1101 3 0.555833993163283 1101 4 0.4035178490464091 1101 5 0.36144320428083887 1101 6 0.386967148987587 1101 7 0.3179388310521105 1101 8 0.39244683085006926 1101 9 0.46561710197509854 1101 10-11 0.529693390433863 1101 12-13 0.5341100060333375 1101 14-15 0.6305640990508081 1101 16-17 0.6520254346873173 1101 18-19 0.6821673953893992 1101 20-21 0.7926553277183785 1101 22-23 0.7383782255390088 1101 24-25 0.9494556498592246 1101 26-27 0.9519853627039794 1101 28-29 0.9017332793633557 1101 30-31 0.9253496239967305 1101 32-33 1.1298698533901543 1101 34-35 1.0495051729283276 1101 36-37 1.0006533674162128 1101 38-39 1.065379512990205 1101 40-41 0.9878629043005347 1101 42-43 0.9093045615499165 1101 44-45 0.8162557432734872 1101 46-47 0.728720203396243 1101 48-49 0.662110686554648 1101 50-51 0.6880770035593002 1101 52-53 0.5813641303197699 1101 54-55 0.5904516321523374 1101 56-57 0.6869939361710706 1101 58-59 0.6537232331738423 1101 60-61 0.510966951672593 1101 62-63 0.4983939337720713 1101 64-65 0.5912414608191696 1101 66-67 0.5981630060145946 1101 68-69 0.5543411073867048 1101 70-71 0.49420469114473065 1101 72-73 0.4652781354075728 1101 74-75 0.5074730811308612 1101 76-77 0.5227215258507982 1101 78-79 0.5301611025663959 1101 80-81 0.5338848856553753 1101 82-83 0.5188132851581315 1101 84-85 0.4607291612506117 1101 86-87 0.49569661867635517 1101 88-89 0.559806393148282 1101 90-91 0.5587590675807448 1101 92-93 0.5009125187592183 1101 94-95 0.5165520668605446 1101 96-97 0.5442890193980894 1101 98-99 0.5021171351594127 1101 100-101 0.38562022479474223 1101 102-103 0.30703813601590113 1101 104-105 0.4081858298436778 1101 106-107 0.4592204797103143 1101 108-109 0.43086717590037793 1101 110-111 0.43840608635892053 1101 112-113 0.4991770583831361 1101 114-115 0.531269673221118 1101 116-117 0.5303702446687026 1101 118-119 0.39728438864628046 1101 120-121 0.3853067395624521 1101 122-123 0.3867611618556026 1101 124-125 0.2937334484981662 1102 1 0.2030351558158081 1102 2 0.2827960930687894 1102 3 0.13060335039382664 1102 4 0.20393932710874907 1102 5 0.23562503338069973 1102 6 0.2683741742715 1102 7 0.30122853920882875 1102 8 0.332531905909633 1102 9 0.31251062751371705 1102 10-11 0.3204668246499125 1102 12-13 0.3734338973195577 1102 14-15 0.4429514818702813 1102 16-17 0.31574784931896716 1102 18-19 0.4166897533918359 1102 20-21 0.4817533682440569 1102 22-23 0.5226913457412863 1102 24-25 0.682355286501668 1102 26-27 0.6940215807471475 1102 28-29 0.4757277627867822 1102 30-31 0.6245238574642045 1102 32-33 0.8276152827879528 1102 34-35 0.6981097439083257 1102 36-37 0.5997685657198844 1102 38-39 0.6571090026949378 1102 40-41 0.6393599795843841 1102 42-43 0.5143488269866481 1102 44-45 0.42716271347912027 1102 46-47 0.41219086457018506 1102 48-49 0.43862933582055774 1102 50-51 0.3501844515341901 1102 52-53 0.3884095168082169 1102 54-55 0.3717009983544699 1102 56-57 0.38599010972212966 1102 58-59 0.345076550178554 1102 60-61 0.3687425627730363 1102 62-63 0.3999270982517089 1102 64-65 0.3010059345750662 1102 66-67 0.2437092910544152 1102 68-69 0.3311249078514251 1102 70-71 0.3014856515985258 1102 72-73 0.2914260899753529 1102 74-75 0.31070265653500684 1102 76-77 0.20451781735287966 1102 78-79 0.23870102914725067 1102 80-81 0.30682886528158093 1102 82-83 0.19033310359468203 1102 84-85 0.24394568688110496 1102 86-87 0.1820211597669399 1102 88-89 0.21731609746343494 1102 90-91 0.1879420340370821 1102 92-93 0.2001412836974623 1102 94-95 0.23394334095033287 1102 96-97 0.24561945803989715 1102 98-99 0.23154261504629758 1102 100-101 0.2478390322963655 1102 102-103 0.1661181279669144 1102 104-105 0.2927127810164869 1102 106-107 0.2669360515122996 1102 108-109 0.19447001897341565 1102 110-111 0.1883153662730841 1102 112-113 0.25548431642744873 1102 114-115 0.2930856040062153 1102 116-117 0.30435201236722165 1102 118-119 0.12835739500156151 1102 120-121 0.09559386043202522 1102 122-123 0.15257782357118543 1102 124-125 0.21007590891835548 1103 1 0.20404041408985663 1103 2 -0.04368086331846399 1103 3 0.17149773284403835 1103 4 0.23559009205676062 1103 5 0.276412346902319 1103 6 0.2208772078911423 1103 7 0.2494845053274588 1103 8 0.1885777314110726 1103 9 0.4654526437406119 1103 10-11 0.31987794554262905 1103 12-13 0.2658742361332287 1103 14-15 0.399927617395754 1103 16-17 0.33151969732868025 1103 18-19 0.48671580683112836 1103 20-21 0.5831833332682095 1103 22-23 0.5429422439306251 1103 24-25 0.6741615393270948 1103 26-27 0.6777233105051721 1103 28-29 0.6052752181152457 1103 30-31 0.6206485570763718 1103 32-33 0.780475212836258 1103 34-35 0.718595598913204 1103 36-37 0.5910930083666273 1103 38-39 0.7056171756231606 1103 40-41 0.6793323795939088 1103 42-43 0.6377071034148258 1103 44-45 0.5372295720767539 1103 46-47 0.527227505936871 1103 48-49 0.4201569715006812 1103 50-51 0.4439381692053175 1103 52-53 0.3353925523313066 1103 54-55 0.35092962620667123 1103 56-57 0.41445557404438205 1103 58-59 0.42755828897794856 1103 60-61 0.38989759858164774 1103 62-63 0.3578490093131208 1103 64-65 0.3337779491364401 1103 66-67 0.4101687595166723 1103 68-69 0.36130347503122806 1103 70-71 0.3078770718083774 1103 72-73 0.2630082118435908 1103 74-75 0.2720388767638937 1103 76-77 0.3378424265512905 1103 78-79 0.2695041650772083 1103 80-81 0.33894359558684783 1103 82-83 0.21855766282759248 1103 84-85 0.2409626275649117 1103 86-87 0.29768534402066393 1103 88-89 0.31606636513575737 1103 90-91 0.28196937230351793 1103 92-93 0.2477810086492802 1103 94-95 0.1992113701682534 1103 96-97 0.21377537419302683 1103 98-99 0.2760832893426155 1103 100-101 0.2562974776555187 1103 102-103 0.27691365010639046 1103 104-105 0.3165088503971525 1103 106-107 0.3590272266532786 1103 108-109 0.3583925486553028 1103 110-111 0.3128751940110739 1103 112-113 0.35037118025956104 1103 114-115 0.29395702611952146 1103 116-117 0.3568009166951853 1103 118-119 0.15594251479979349 1103 120-121 0.14911940231977283 1103 122-123 0.12990002966697745 1103 124-125 0.1590516161681137 1104 1 0.30475750165740223 1104 2 0.3981430598530231 1104 3 0.47954445439827964 1104 4 0.41523850656332684 1104 5 0.3320715294798049 1104 6 0.4011870836272493 1104 7 0.3701414882702707 1104 8 0.44787586468713414 1104 9 0.5364107352973946 1104 10-11 0.5406198293381408 1104 12-13 0.5941776842331308 1104 14-15 0.6661555757092188 1104 16-17 0.6057173209616167 1104 18-19 0.7483673730843066 1104 20-21 0.7256749628990491 1104 22-23 0.7411836881324021 1104 24-25 0.919937798066961 1104 26-27 0.9027803529680725 1104 28-29 0.701800718787986 1104 30-31 0.7086343724077793 1104 32-33 0.8751401395971286 1104 34-35 0.8411790829269847 1104 36-37 0.7575588471024162 1104 38-39 0.8697326754362322 1104 40-41 0.7803530176983529 1104 42-43 0.6449561396644867 1104 44-45 0.6010076485973883 1104 46-47 0.5785438737607151 1104 48-49 0.5031812087996101 1104 50-51 0.5688022569157667 1104 52-53 0.5624171753874165 1104 54-55 0.5910958883138235 1104 56-57 0.5861436053946747 1104 58-59 0.5962816450045025 1104 60-61 0.5674183508805086 1104 62-63 0.611576273844392 1104 64-65 0.5563344244353701 1104 66-67 0.6140077765327305 1104 68-69 0.5761141993474652 1104 70-71 0.6050776094693546 1104 72-73 0.47827361935387813 1104 74-75 0.45217872231059886 1104 76-77 0.42417158995610293 1104 78-79 0.44678605637651003 1104 80-81 0.3718989401945336 1104 82-83 0.4350174705373462 1104 84-85 0.5020649277611184 1104 86-87 0.5086768151883163 1104 88-89 0.5688617956838975 1104 90-91 0.5592000736628151 1104 92-93 0.5368879284114989 1104 94-95 0.5990634458075057 1104 96-97 0.5770288799708645 1104 98-99 0.5032050181060015 1104 100-101 0.5629758655522643 1104 102-103 0.5563872552995264 1104 104-105 0.621826996778676 1104 106-107 0.560868960153897 1104 108-109 0.45643227955063637 1104 110-111 0.4488875881265848 1104 112-113 0.5276524527479829 1104 114-115 0.6032765464515961 1104 116-117 0.5549849066868262 1104 118-119 0.27837362853466274 1104 120-121 0.3377223434612411 1104 122-123 0.4217858386865174 1104 124-125 0.44863839996711263 1105 1 0.3181041297446079 1105 2 0.34337416976401514 1105 3 0.29423209170722586 1105 4 0.32161844621538904 1105 5 0.1784431898201504 1105 6 0.18636474159895755 1105 7 0.2570170033321588 1105 8 0.20231099327022406 1105 9 0.27559101240290573 1105 10-11 0.300317947575266 1105 12-13 0.2909933177570352 1105 14-15 0.21784589468143878 1105 16-17 0.3050636684607255 1105 18-19 0.49795852481993563 1105 20-21 0.5267628633364438 1105 22-23 0.507624659901893 1105 24-25 0.6811389132784811 1105 26-27 0.6841881574858348 1105 28-29 0.5882444160568028 1105 30-31 0.5939623067101607 1105 32-33 0.7893952861502029 1105 34-35 0.7977867690780727 1105 36-37 0.6483897477219784 1105 38-39 0.7482962587836539 1105 40-41 0.6141745294588006 1105 42-43 0.4940145572279846 1105 44-45 0.4270127909678081 1105 46-47 0.46747220574672355 1105 48-49 0.48212069794794843 1105 50-51 0.31516131789712887 1105 52-53 0.33513025386787376 1105 54-55 0.357686594305072 1105 56-57 0.4263912108388297 1105 58-59 0.3709027132076912 1105 60-61 0.35379631276968837 1105 62-63 0.37173439019161947 1105 64-65 0.40296181063759207 1105 66-67 0.40201468225260584 1105 68-69 0.4317787748757169 1105 70-71 0.3895327203740919 1105 72-73 0.3804132858652949 1105 74-75 0.3670218233717648 1105 76-77 0.3459089144448413 1105 78-79 0.3724371268500164 1105 80-81 0.2672444397605247 1105 82-83 0.3593615444503513 1105 84-85 0.3924825637979694 1105 86-87 0.3889903354688471 1105 88-89 0.4329419578903604 1105 90-91 0.3922668389559192 1105 92-93 0.4261613407637519 1105 94-95 0.49816300289517557 1105 96-97 0.40297668030317624 1105 98-99 0.3252236971230289 1105 100-101 0.38057406694703744 1105 102-103 0.2407495253787495 1105 104-105 0.36165612217847 1105 106-107 0.3854284475880476 1105 108-109 0.3599842799932951 1105 110-111 0.3073379959560576 1105 112-113 0.3197989675905042 1105 114-115 0.3483289380071497 1105 116-117 0.4154478004062767 1105 118-119 0.3517503137573428 1105 120-121 0.38872343637151374 1105 122-123 0.34190693086400614 1105 124-125 0.1543076067879241 1106 1 0.2006608471806004 1106 2 0.26180351745465913 1106 3 0.28532935337584675 1106 4 0.1860389679631851 1106 5 0.1364178750001912 1106 6 0.18634314286829579 1106 7 0.16000556615816208 1106 8 0.18181859759256014 1106 9 0.24045153906119054 1106 10-11 0.3373428331896804 1106 12-13 0.2924414941872868 1106 14-15 0.332460943458571 1106 16-17 0.33529972228032534 1106 18-19 0.3700657025211882 1106 20-21 0.4599681426569475 1106 22-23 0.4379541697676217 1106 24-25 0.6292915295401045 1106 26-27 0.6606258345331213 1106 28-29 0.5547878899634142 1106 30-31 0.6172643834188918 1106 32-33 0.7037518424264348 1106 34-35 0.7754839567202723 1106 36-37 0.583272113096811 1106 38-39 0.6369439567189445 1106 40-41 0.5580142761075351 1106 42-43 0.45697896455051534 1106 44-45 0.44629593463535855 1106 46-47 0.3795723407849394 1106 48-49 0.3201476169813233 1106 50-51 0.37496884920836493 1106 52-53 0.3496948133045379 1106 54-55 0.3854776995047331 1106 56-57 0.3215494492315898 1106 58-59 0.35440829795641093 1106 60-61 0.2937463957032733 1106 62-63 0.35703239267824216 1106 64-65 0.3793497829136854 1106 66-67 0.35790924352608045 1106 68-69 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2216 80-81 -2.282771006817203 2216 82-83 -2.273428540423879 2216 84-85 -2.2653008260130356 2216 86-87 -2.310761282324375 2216 88-89 -2.2061223134795647 2216 90-91 -2.3004171577710792 2216 92-93 -2.320648763122602 2216 94-95 -2.1931483125966125 2216 96-97 -2.337850851157434 2216 98-99 -2.2904286880821445 2216 100-101 -2.3137816468642143 2216 102-103 -2.2865685358917496 2216 104-105 -2.280293481776841 2216 106-107 -2.277703686983653 2216 108-109 -2.3050795658612984 2216 110-111 -2.3753254393504584 2216 112-113 -2.384857247871164 2216 114-115 -2.432207856081156 2216 116-117 -2.367767096279465 2216 118-119 -2.219078352616986 2216 120-121 -2.153701462955148 2216 122-123 -2.2379762673414056 2216 124-125 -2.0747285344930617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33849.0 3 6729.0 4 4550.0 5 3684.0 6 3812.0 7 4412.0 8 4592.0 9 4938.0 10 5592.0 11 6458.0 12 7013.0 13 7141.0 14 7172.0 15 7008.0 16 6538.0 17 6013.0 18 5422.0 19 5324.0 20 5256.0 21 5674.0 22 6542.0 23 8979.0 24 13427.0 25 20246.0 26 23994.0 27 23626.0 28 26852.0 29 33005.0 30 39227.0 31 47581.0 32 61758.0 33 82150.0 34 117884.0 35 184340.0 36 372437.0 37 712485.0 38 294468.0 39 973.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.767061941231944 18.117842021710835 12.518214548831283 24.596881488225936 2 16.094684516165813 20.149373267560588 39.16713498525362 24.588807231019974 3 19.308106865676773 24.353626627103544 28.763620749645156 27.574645757574523 4 12.922326683911214 16.40197324455573 36.113017704092044 34.56268236744101 5 14.224141831495468 37.770816026987944 33.71588274577725 14.289159395739336 6 33.03691482332254 35.787899304542655 17.271104441619258 13.90408143051555 7 28.902103749773307 32.121835409405165 20.688945601536215 18.287115239285313 8 25.801856137369178 35.9169952662663 19.66220307884898 18.618945517515538 9 26.164742765244224 16.730751488377898 19.2959081592596 37.80859758711827 10-11 25.08378304059396 26.77486626372414 27.435067578202027 20.706283117479877 12-13 25.369900979083475 24.27639474225142 27.3170326875985 23.03667159106661 14-15 22.638440680879164 25.857397109406975 24.66287345118534 26.841288758528513 16-17 23.251501126480857 28.827440047089908 25.30824735950309 22.612811466926146 18-19 23.00746571411385 27.716378845982504 26.77220820418979 22.503947235713856 20-21 23.76387620885024 27.1324533279721 26.47625991464691 22.627410548530747 22-23 23.553186869851952 26.973163439313545 26.301684013406973 23.171965677427526 24-25 23.427449025880197 27.023508495648947 26.538879539481506 23.01016293898935 26-27 23.200944994507662 27.413887194559994 26.34301102335279 23.042156787579554 28-29 23.103595814900764 27.281548919102644 26.540522400176936 23.074332865819656 30-31 23.209178126057218 26.949166800844505 26.581368758695177 23.2602863144031 32-33 23.080629893767952 27.098375788011687 26.56882108160497 23.252173236615388 34-35 23.629488918959325 27.141374443030386 26.175428946610758 23.053707691399534 36-37 23.547274597018323 26.958503511056964 26.21558290059058 23.278638991334137 38-39 23.146682711283972 27.039078218640334 26.543715738842188 23.27052333123351 40-41 23.358042067790684 26.864693842430203 26.525733635485597 23.251530454293516 42-43 23.40188144171956 26.889814160086488 26.338284586998416 23.37001981119553 44-45 23.239600641477313 27.1304789462116 26.31705459521895 23.31286581709213 46-47 23.283458696433307 27.201376864702148 26.092477060734865 23.422687378129677 48-49 23.373187034250577 27.06282023276334 26.21672055418896 23.347272178797127 50-51 23.165660048061714 27.11217482759268 26.261495611604186 23.46066951274142 52-53 23.52611958017637 26.940341067124834 26.18499954095641 23.34853981174238 54-55 23.241955840028368 26.679969670237323 26.455594554482424 23.62247993525188 56-57 23.181942040226538 27.216829613281156 26.31942662088257 23.281801725609736 58-59 23.30668375713117 26.87442375652963 26.216208698452427 23.60268378788677 60-61 23.34885860528568 26.80416264852063 26.58740892453605 23.259569821657642 62-63 23.227613501441738 27.02237801775315 26.366710013003903 23.38329846780121 64-65 23.054845626401736 26.827452617411176 26.39743982285716 23.720261933329926 66-67 23.068173389511312 27.234033507788997 26.12384603277692 23.57394706992277 68-69 22.98842412563556 26.848036429844484 26.5544501657557 23.60908927876425 70-71 22.924887510588636 26.852033176292128 26.538591477443095 23.684487835676137 72-73 23.16783939779601 26.782931221784125 26.429755078744392 23.61947430167547 74-75 22.947043319066104 26.997690771407175 26.39189137851811 23.663374531008607 76-77 23.132087674033137 26.798152088623766 26.41823580090858 23.651524436434517 78-79 23.144989392057 26.87913909905526 26.474992526273372 23.50087898261437 80-81 23.018946260135245 27.12351702504098 26.435555036959045 23.421981677864736 82-83 23.076482993452636 27.018717671911823 26.381393312616826 23.523406022018715 84-85 23.133283504624234 26.815029574959603 26.293773032114643 23.757913888301523 86-87 23.179648865084697 26.60443093221987 26.63131919374112 23.584601008954312 88-89 23.039054845115018 26.68715714312527 26.62560430661815 23.648183705141562 90-91 23.016712024268106 26.849679971688715 26.66172199088024 23.471886013162937 92-93 23.039393931170974 26.569715010885975 26.69431351425669 23.696577543686363 94-95 22.93185766146229 26.56204845349775 26.645149969405075 23.860943915634888 96-97 23.065858357078877 26.504257012237936 26.66413099445461 23.765753636228578 98-99 22.887665845398466 26.447278811167347 26.59694368805289 24.068111655381298 100-101 22.73320430121145 26.538943260393545 26.831346211517086 23.89650622687791 102-103 22.966449722072717 26.37045965418519 26.77170162052637 23.891389003215725 104-105 22.94799494495812 26.456422879401792 26.763565523311723 23.832016652328367 106-107 22.89905426282943 26.535222545686505 26.724890237033723 23.84083295445035 108-109 22.911534007665324 26.651347175297296 26.433811000863134 24.003307816174242 110-111 22.704539487802446 26.766282871640364 26.766961261026374 23.76221637953082 112-113 22.818941912406444 26.62025189632396 26.800911257760518 23.759894933509077 114-115 22.541625395081883 26.824581042991287 26.68255174998682 23.951241811940005 116-117 22.583520563493593 26.711829650606013 26.85730018897596 23.847349596924442 118-119 22.67644511074392 26.844357117455935 26.410785881622456 24.06841189017769 120-121 22.55100072625324 26.888982865132423 26.68473546089914 23.8752809477152 122-123 22.736528228641788 26.824102234358012 26.690028540417405 23.749340996582795 124-125 22.717251986670085 26.874910676409268 26.485516534221993 23.92232080269865 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1218.0 1 1041.5 2 778.0 3 619.0 4 487.0 5 399.5 6 365.5 7 347.5 8 349.5 9 381.5 10 364.0 11 333.0 12 350.0 13 365.0 14 382.5 15 431.0 16 511.0 17 626.5 18 776.0 19 972.0 20 1244.0 21 1779.0 22 2469.0 23 3232.5 24 4372.5 25 6075.5 26 8438.0 27 11293.5 28 14044.5 29 17057.5 30 21223.5 31 25609.5 32 29836.5 33 33786.5 34 37699.0 35 41467.0 36 45810.0 37 50837.5 38 53983.5 39 56327.5 40 58586.5 41 63434.5 42 69581.5 43 74475.0 44 78029.5 45 79992.5 46 80749.5 47 80915.0 48 85108.0 49 88743.5 50 88655.0 51 87680.0 52 86426.0 53 86619.5 54 85053.0 55 78745.0 56 71158.0 57 64534.5 58 59328.5 59 54035.5 60 46473.0 61 37591.5 62 29709.5 63 23146.0 64 17855.0 65 13788.0 66 10483.0 67 8683.5 68 6548.5 69 4233.0 70 3258.0 71 2579.0 72 2042.0 73 1674.0 74 1278.0 75 1060.0 76 773.5 77 409.0 78 206.5 79 114.5 80 67.0 81 45.5 82 32.0 83 28.5 84 24.5 85 17.5 86 16.5 87 14.5 88 12.5 89 11.5 90 9.0 91 7.0 92 8.0 93 6.0 94 3.0 95 3.0 96 4.0 97 3.5 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.062365709080926625 2 0.05051667660869837 3 0.045768018556851156 4 0.04450170974302524 5 0.044275583169127754 6 0.003979827700595753 7 1.8090125911798878E-4 8 0.0 9 8.592809808104467E-4 10-11 0.0037537011266982672 12-13 0.0023743290259236024 14-15 0.008411908548986479 16-17 0.0027813568589390774 18-19 0.004974784625744692 20-21 0.009836505964540639 22-23 0.016326338635398487 24-25 0.0035275745528007807 26-27 0.015580120941536784 28-29 0.0074169516238375395 30-31 0.007009923790822065 32-33 0.0021708151094158652 34-35 0.018791118290881086 36-37 0.01580624751543427 38-39 0.029328616634503934 40-41 0.0056305516900474 42-43 0.001243696156436173 44-45 2.2612657389748598E-4 46-47 0.0018316252485696364 48-49 0.0028944201458878205 50-51 0.0016054986746721505 52-53 0.0014472100729439102 54-55 0.005494875745708909 56-57 0.002193427766805614 58-59 0.007756141484683769 60-61 0.015693184228485528 62-63 0.012482186879141226 64-65 0.0024421669980928488 66-67 0.0037537011266982672 68-69 0.009791280649761144 70-71 0.0024421669980928488 72-73 0.003301447978903295 74-75 0.0010401822399284354 76-77 2.7135188867698316E-4 78-79 0.003256222664123798 80-81 0.002487392312872346 82-83 0.0014472100729439102 84-85 0.011012364148807567 86-87 0.006738571902145082 88-89 0.0019220758781286307 90-91 8.36668323420698E-4 92-93 0.0022160404241953627 94-95 0.0 96-97 0.0015602733598926533 98-99 0.024670409212215718 100-101 0.015014804506793069 102-103 0.006376769383909104 104-105 0.01307011597127469 106-107 0.03192907223432502 108-109 0.0011080202120976814 110-111 0.0014245974155541617 112-113 0.016303725978008738 114-115 0.03127330517002231 116-117 0.04891117793402621 118-119 0.35897593606225897 120-121 0.11555067926161532 122-123 0.019288596753455553 124-125 0.3204665805275171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2211151.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.23665384257296 #Duplication Level Percentage of deduplicated Percentage of total 1 86.99207503891051 68.05968861866373 2 8.641665491595402 13.521899833785152 3 2.218762799742849 5.207657313667779 4 0.8476110775970578 2.6525701788436504 5 0.410349021663141 1.6052167181248818 6 0.2392191305700234 1.1229422586556908 7 0.1399063932933873 0.7662065643720328 8 0.09682304518578286 0.6060088856147114 9 0.06624351906183436 0.46644041431391564 >10 0.32555198184119344 4.393848883513737 >50 0.017270917305926385 0.94526929804234 >100 0.004413927774239755 0.5055292907307221 >500 0.0 0.0 >1k 1.0765545863402189E-4 0.14672174167162144 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 2.2612657389748597E-5 0.0 0.0 0.0 16-17 0.0 4.5225314779497195E-5 0.0 6.783797216924579E-5 0.0 18-19 0.0 6.783797216924579E-5 0.0 1.1306328694874298E-4 0.0 20-21 0.0 9.045062955899439E-5 0.0 1.3567594433849158E-4 0.0 22-23 4.5225314779497195E-5 9.045062955899439E-5 0.0 3.1657720345648036E-4 0.0 24-25 4.5225314779497195E-5 1.1306328694874298E-4 0.0 7.236050364719551E-4 0.0 26-27 4.5225314779497195E-5 1.3567594433849158E-4 0.0 0.001175858184266927 0.0 28-29 4.5225314779497195E-5 1.3567594433849158E-4 0.0 0.002849194831108323 0.0 30-31 4.5225314779497195E-5 1.3567594433849158E-4 0.0 0.008751098409832708 0.0 32-33 4.5225314779497195E-5 1.3567594433849158E-4 0.0 0.021368961233312424 4.5225314779497195E-5 34-35 6.783797216924579E-5 1.8090125911798878E-4 0.0 0.04296404904052233 4.5225314779497195E-5 36-37 9.045062955899439E-5 2.0351391650773738E-4 0.0 0.07794583002246341 4.5225314779497195E-5 38-39 1.8090125911798878E-4 2.2612657389748598E-4 0.0 0.12597511431828945 4.5225314779497195E-5 40-41 1.8090125911798878E-4 2.7135188867698316E-4 0.0 0.17414007455845396 4.5225314779497195E-5 42-43 1.8090125911798878E-4 3.1657720345648036E-4 0.0 0.22268944997424417 4.5225314779497195E-5 44-45 1.8090125911798878E-4 3.1657720345648036E-4 0.0 0.28824354374712535 4.5225314779497195E-5 46-47 2.2612657389748598E-4 3.1657720345648036E-4 0.0 0.3688576673415791 4.5225314779497195E-5 48-49 2.2612657389748598E-4 3.39189860846229E-4 0.0 0.4527054009427669 4.5225314779497195E-5 50-51 2.2612657389748598E-4 3.6180251823597756E-4 0.0 0.5407138635036685 4.5225314779497195E-5 52-53 2.2612657389748598E-4 3.6180251823597756E-4 0.0 0.6276143058524724 4.5225314779497195E-5 54-55 2.2612657389748598E-4 4.0702783301547476E-4 0.0 0.7275622515151612 4.5225314779497195E-5 56-57 2.2612657389748598E-4 4.0702783301547476E-4 0.0 0.8494218621885163 4.5225314779497195E-5 58-59 2.2612657389748598E-4 4.0702783301547476E-4 0.0 0.9876982621268289 4.5225314779497195E-5 60-61 2.2612657389748598E-4 4.0702783301547476E-4 0.0 1.137371441389575 4.5225314779497195E-5 62-63 2.2612657389748598E-4 4.0702783301547476E-4 0.0 1.2820472233691864 4.5225314779497195E-5 64-65 2.2612657389748598E-4 4.0702783301547476E-4 0.0 1.4406071769861035 9.045062955899439E-5 66-67 2.2612657389748598E-4 4.974784625744692E-4 0.0 1.6272746637384783 9.045062955899439E-5 68-69 2.7135188867698316E-4 4.974784625744692E-4 0.0 1.8475445593720194 9.045062955899439E-5 70-71 2.7135188867698316E-4 4.974784625744692E-4 0.0 2.0905627883396476 9.045062955899439E-5 72-73 2.7135188867698316E-4 4.974784625744692E-4 0.0 2.334168946399409 9.045062955899439E-5 74-75 2.7135188867698316E-4 4.974784625744692E-4 0.0 2.588448278747132 9.045062955899439E-5 76-77 2.7135188867698316E-4 4.974784625744692E-4 0.0 2.8705185670268563 9.045062955899439E-5 78-79 2.7135188867698316E-4 4.974784625744692E-4 0.0 3.183364682014028 9.045062955899439E-5 80-81 2.7135188867698316E-4 4.974784625744692E-4 0.0 3.5311021273535816 9.045062955899439E-5 82-83 2.7135188867698316E-4 5.427037773539664E-4 0.0 3.889806711527164 9.045062955899439E-5 84-85 2.7135188867698316E-4 5.879290921334636E-4 0.0 4.261355285098123 9.045062955899439E-5 86-87 2.7135188867698316E-4 5.879290921334636E-4 0.0 4.650993984580881 9.045062955899439E-5 88-89 2.7135188867698316E-4 5.879290921334636E-4 0.0 5.087056469684793 9.045062955899439E-5 90-91 2.7135188867698316E-4 5.879290921334636E-4 0.0 5.56223885207297 9.045062955899439E-5 92-93 2.7135188867698316E-4 6.105417495232122E-4 0.0 6.056619380585044 9.045062955899439E-5 94-95 2.7135188867698316E-4 6.331544069129607E-4 0.0 6.557512354425365 9.045062955899439E-5 96-97 2.7135188867698316E-4 6.331544069129607E-4 0.0 7.063018310373195 9.045062955899439E-5 98-99 2.7135188867698316E-4 6.331544069129607E-4 0.0 7.602872892896053 9.045062955899439E-5 100-101 2.7135188867698316E-4 6.557670643027092E-4 0.0 8.204279128833807 9.045062955899439E-5 102-103 2.7135188867698316E-4 6.783797216924579E-4 0.0 8.83707173322853 9.045062955899439E-5 104-105 3.1657720345648036E-4 6.783797216924579E-4 0.0 9.470407041400609 1.1306328694874298E-4 106-107 3.1657720345648036E-4 6.783797216924579E-4 0.0 10.108830197485382 1.3567594433849158E-4 108-109 3.1657720345648036E-4 6.783797216924579E-4 0.0 10.760391307513599 1.3567594433849158E-4 110-111 3.1657720345648036E-4 7.23605036471955E-4 0.0 11.45717773232131 1.3567594433849158E-4 112-113 3.1657720345648036E-4 7.688303512514523E-4 0.0 12.19403378602366 1.3567594433849158E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2150 0.0 54.796097 1 GTATCAA 3285 0.0 44.0143 1 TCAACGC 3735 0.0 36.791374 4 CAACGCA 3730 0.0 36.68287 5 ATCAACG 3765 0.0 36.34186 3 AACGCAG 3880 0.0 35.406025 6 TATCAAC 4265 0.0 33.478497 2 GTACATG 8405 0.0 33.201527 1 TACATGG 8580 0.0 31.411211 2 ACATGGG 8730 0.0 30.392393 3 ACGCAGA 4980 0.0 26.868305 7 CGCAGAG 5105 0.0 25.860945 8 CATGGGG 5440 0.0 24.494795 4 CATGGGA 4245 0.0 22.561752 4 GTGTAGG 1165 0.0 20.9402 1 GCAGAGT 6260 0.0 20.425877 9 ATGGGGA 3405 0.0 20.2668 5 TGGTATC 1375 0.0 19.903475 2 CAGAGTA 5950 0.0 19.341074 10-11 GAGTACA 6790 0.0 18.928093 1 >>END_MODULE